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Protein

Transcription initiation factor TFIID subunit 12

Gene

Taf12

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors.

GO - Molecular functioni

  • protein homodimerization activity Source: FlyBase
  • RNA polymerase II transcription factor activity, TBP-class protein binding, involved in preinitiation complex assembly Source: FlyBase
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • histone H3 acetylation Source: FlyBase
  • neurogenesis Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • transcription initiation from RNA polymerase II promoter Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-674695. RNA Polymerase II Pre-transcription Events.
R-DME-73776. RNA Polymerase II Promoter Escape.
R-DME-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-DME-75953. RNA Polymerase II Transcription Initiation.
R-DME-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 12
Alternative name(s):
TAFII30 alpha
Transcription initiation factor TFIID 28-alpha kDa/22 kDa subunits
p28-alpha/p22
Gene namesi
Name:Taf12
Synonyms:TAF30-ALPHA
ORF Names:CG17358
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0011290. Taf12.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: FlyBase
  • SAGA complex Source: FlyBase
  • transcription factor TFIID complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196Transcription initiation factor TFIID subunit 12PRO_0000033584Add
BLAST

Proteomic databases

PaxDbiP49905.
PRIDEiP49905.

Expressioni

Gene expression databases

BgeeiFBgn0011290.
GenevisibleiP49905. DM.

Interactioni

Subunit structurei

Belongs to the TFIID complex which is composed of TATA binding protein (Tbp) and a number of TBP-associated factors (TAFs). Interacts with Tbp, Taf2 and Taf4.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: FlyBase
  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi66532. 38 interactions.
DIPiDIP-779N.
IntActiP49905. 6 interactions.
MINTiMINT-319520.
STRINGi7227.FBpp0081916.

Structurei

3D structure databases

ProteinModelPortaliP49905.
SMRiP49905. Positions 91-162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TAF12 family.Curated
Contains 1 histone-fold domain.Curated

Phylogenomic databases

eggNOGiKOG1142. Eukaryota.
COG5624. LUCA.
GeneTreeiENSGT00390000002144.
InParanoidiP49905.
KOiK03126.
OMAiLHDPPMA.
OrthoDBiEOG091G0Q1X.
PhylomeDBiP49905.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR003228. TFIID_sub.
[Graphical view]
PfamiPF03847. TFIID_20kDa. 1 hit.
[Graphical view]
ProDomiPD012998. PD012998. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 28 kDa (identifier: P49905-1) [UniParc]FASTAAdd to basket
Also known as: A, B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDLFTTFDS NGVAEHHLHH NHNSTSSASG LLHDPPMASP SQHSPMTNNS
60 70 80 90 100
NSSSQNGGPV SGLGTGTGPI SGGSKSSNHT SSAAGSENTP MLTKPRLTEL
110 120 130 140 150
VREVDTTTQL DEDVEELLLQ IIDDFVEDTV KSTSAFAKHR KSNKIEVRDV
160 170 180 190
QLHFERKYNM WIPGFGTDEL RPYKRAAVTE AHKQRLALIR KTIKKY
Length:196
Mass (Da):21,523
Last modified:October 1, 1996 - v1
Checksum:i9D6AE98FB9AEC1EC
GO
Isoform 22 kDa (identifier: P49905-2) [UniParc]FASTAAdd to basket
Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Show »
Length:160
Mass (Da):17,630
Checksum:iE3517F6FC3CA3FAE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151E → R in AAB29540 (PubMed:8276241).Curated
Sequence conflicti127 – 1271E → R in AAB29540 (PubMed:8276241).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3636Missing in isoform 22 kDa. 1 PublicationVSP_018890Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06450 mRNA. Translation: AAC46479.1.
U06456 mRNA. Translation: AAB19244.1.
S67741 mRNA. Translation: AAB29540.1.
AE014297 Genomic DNA. Translation: AAF54677.1.
AE014297 Genomic DNA. Translation: AAF54678.1.
AE014297 Genomic DNA. Translation: AAF54679.2.
AY061435 mRNA. Translation: AAL28983.1.
PIRiA49453.
S42222.
RefSeqiNP_001262479.1. NM_001275550.1. [P49905-2]
NP_524320.1. NM_079596.3. [P49905-1]
NP_731616.1. NM_169420.2. [P49905-1]
NP_731617.2. NM_169421.3. [P49905-2]
UniGeneiDm.2806.

Genome annotation databases

EnsemblMetazoaiFBtr0082440; FBpp0081916; FBgn0011290. [P49905-1]
FBtr0082442; FBpp0081918; FBgn0011290. [P49905-1]
GeneIDi41406.
KEGGidme:Dmel_CG17358.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06450 mRNA. Translation: AAC46479.1.
U06456 mRNA. Translation: AAB19244.1.
S67741 mRNA. Translation: AAB29540.1.
AE014297 Genomic DNA. Translation: AAF54677.1.
AE014297 Genomic DNA. Translation: AAF54678.1.
AE014297 Genomic DNA. Translation: AAF54679.2.
AY061435 mRNA. Translation: AAL28983.1.
PIRiA49453.
S42222.
RefSeqiNP_001262479.1. NM_001275550.1. [P49905-2]
NP_524320.1. NM_079596.3. [P49905-1]
NP_731616.1. NM_169420.2. [P49905-1]
NP_731617.2. NM_169421.3. [P49905-2]
UniGeneiDm.2806.

3D structure databases

ProteinModelPortaliP49905.
SMRiP49905. Positions 91-162.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66532. 38 interactions.
DIPiDIP-779N.
IntActiP49905. 6 interactions.
MINTiMINT-319520.
STRINGi7227.FBpp0081916.

Proteomic databases

PaxDbiP49905.
PRIDEiP49905.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0082440; FBpp0081916; FBgn0011290. [P49905-1]
FBtr0082442; FBpp0081918; FBgn0011290. [P49905-1]
GeneIDi41406.
KEGGidme:Dmel_CG17358.

Organism-specific databases

CTDi6883.
FlyBaseiFBgn0011290. Taf12.

Phylogenomic databases

eggNOGiKOG1142. Eukaryota.
COG5624. LUCA.
GeneTreeiENSGT00390000002144.
InParanoidiP49905.
KOiK03126.
OMAiLHDPPMA.
OrthoDBiEOG091G0Q1X.
PhylomeDBiP49905.

Enzyme and pathway databases

ReactomeiR-DME-674695. RNA Polymerase II Pre-transcription Events.
R-DME-73776. RNA Polymerase II Promoter Escape.
R-DME-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-DME-75953. RNA Polymerase II Transcription Initiation.
R-DME-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

GenomeRNAii41406.
PROiP49905.

Gene expression databases

BgeeiFBgn0011290.
GenevisibleiP49905. DM.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR003228. TFIID_sub.
[Graphical view]
PfamiPF03847. TFIID_20kDa. 1 hit.
[Graphical view]
ProDomiPD012998. PD012998. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTAF12_DROME
AccessioniPrimary (citable) accession number: P49905
Secondary accession number(s): A4V2Q1, Q26339, Q9VGK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.