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Reviewed, UniProtKB/Swiss-Prot P49903 (SPS1_HUMAN)

Last modified June 16, 2009. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Selenide, water dikinase 1
    EC=2.7.9.3
Alternative name(s):
    Selenophosphate synthetase 1
    Selenium donor protein 1
Gene names
Name: SEPHS1
Synonyms: SELD, SPS, SPS1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length392 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Synthesizes selenophosphate from selenide and ATP. Ref.1

Catalytic activity

ATP + selenide + H2O = AMP + selenophosphate + phosphate.

Sequence similarities

Belongs to the selenophosphate synthetase 1 family. Class II subfamily.

Caution

The conserved active site Cys (or selenocysteine) residue in position 29 is replaced by a Thr. However, as function in selenoprotein synthesis is proven, it is possible Cys-31 is the active site.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 392392Selenide, water dikinase 1
PRO_0000127648

Regions

Nucleotide binding268 – 2747ATP Potential

Sites

Active site311 Potential
Site321Important for catalytic activity By similarity

Experimental info

Mutagenesis2681G → C: No change in ATP-binding. Ref.1
Mutagenesis2701G → R: No change in ATP-binding. Ref.1
Mutagenesis2731G → A, D or V: Loss of ATP-binding. Ref.1
Mutagenesis2741H → N: Reduced ATP-binding. Ref.1
Mutagenesis2741H → Y: Increased ATP-binding. Ref.1
Sequence conflict2601A → T in AAA87567. Ref.1
Sequence conflict377 – 39216PQVAT…PGATS → HKWPLKT in AAA87567. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P49903-1 [UniParc].

Last modified December 13, 2002. Version 2.
Checksum: E9636E38146D926D

FASTA39242,911
        10         20         30         40         50         60 
MSTRESFNPE SYELDKSFRL TRFTELKGTG CKVPQDVLQK LLESLQENHF QEDEQFLGAV 

        70         80         90        100        110        120 
MPRLGIGMDT CVIPLRHGGL SLVQTTDYIY PIVDDPYMMG RIACANVLSD LYAMGVTECD 

       130        140        150        160        170        180 
NMLMLLGVSN KMTDRERDKV MPLIIQGFKD AAEEAGTSVT GGQTVLNPWI VLGGVATTVC 

       190        200        210        220        230        240 
QPNEFIMPDN AVPGDVLVLT KPLGTQVAVA VHQWLDIPEK WNKIKLVVTQ EDVELAYQEA 

       250        260        270        280        290        300 
MMNMARLNRT AAGLMHTFNA HAATDITGFG ILGHAQNLAK QQRNEVSFVI HNLPVLAKMA 

       310        320        330        340        350        360 
AVSKACGNMF GLMHGTCPET SGGLLICLPR EQAARFCAEI KSPKYGEGHQ AWIIGIVEKG 

       370        380        390 
NRTARIIDKP RIIEVAPQVA TQNVNPTPGA TS

« Hide

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References

« Hide 'large scale' references
[1]"Cloning and functional characterization of human selenophosphate synthetase, an essential component of selenoprotein synthesis."
Low S.C., Harney J.W., Berry M.J.
J. Biol. Chem. 270:21659-21664(1995) [PubMed: 7665581] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ATP-BINDING, MUTAGENESIS OF GLY-268; GLY-270; GLY-273 AND HIS-274.
Tissue: Liver.
[2]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed: 15164054] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta and PNS.
[5]Lubec G., Chen W.-Q., Sun Y.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 5-16 AND 64-76, MASS SPECTROMETRY.
Tissue: Fetal brain cortex.
[6]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U34044 mRNA. Translation: AAA87567.1.
AL138764, AL355870 Genomic DNA. Translation: CAI12907.1.
AL355870, AL138764 Genomic DNA. Translation: CAI14198.1.
CH471072 Genomic DNA. Translation: EAW86290.1.
BC000941 mRNA. Translation: AAH00941.1.
BC063816 mRNA. Translation: AAH63816.1.
IPIIPI00029056.
RefSeqNP_036379.2.
UniGeneHs.124027

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActP49903. 11 interactions.

Proteomic databases

PeptideAtlasP49903.
PRIDEP49903.

Genome annotation databases

EnsemblENSG00000086475. Homo sapiens. [Contig view]
GeneID22929.
KEGGhsa:22929.

Organism-specific databases

GeneCardsGC10M013400.
H-InvDBHIX0008655.
HGNCHGNC:19685. SEPHS1.
MIM600902. gene.
PharmGKBPA134905215.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP49903.
HOVERGENP49903.
OMAP49903. MMAIAIL.

Enzyme and pathway databases

BRENDA2.7.9.3. 247.

Gene expression databases

ArrayExpressP49903.
BgeeP49903.
CleanExHS_SEPHS1.
GermOnlineENSG00000086475. Homo sapiens.

Family and domain databases

InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR004536. SelD.
[Graphical view]
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
PIRSFPIRSF036407. Selenphspht_syn. 1 hit.
TIGRFAMsTIGR00476. selD. 1 hit.
ProtoNetSearch...

Other Resources

NextBio43651.
SOURCESearch...

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Entry information

Entry nameSPS1_HUMAN
AccessionPrimary (citable) accession number: P49903
Secondary accession number(s): Q5T5U8, Q9BVT4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 13, 2002
Last modified: June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents