Reviewed, UniProtKB/Swiss-Prot P49903 (SPS1_HUMAN)

Names and origin

Protein names

Recommended name:
    Selenide, water dikinase 1
    EC=2.7.9.3
Alternative name(s):
    Selenophosphate synthetase 1
    Selenium donor protein 1

Gene names

Name:	SEPHS1
Synonyms:	SELD, SPS, SPS1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Synthesizes selenophosphate from selenide and ATP.
Catalytic activity	ATP + selenide + H(2)O = AMP + selenophosphate + phosphate.
Sequence similarities	Belongs to the selenophosphate synthetase 1 family. Class II subfamily.
Caution	The conserved active site Cys (or selenocysteine) residue in position 29 is replaced by a Thr. However, as function in selenoprotein synthesis is proven, it is possible Cys-31 is the active site.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding Selenium
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	protein modification process Ref.1 Traceable author statement. Source: ProtInc
Molecular function	ATP binding Ref.1 Traceable author statement. Source: ProtInc GTP binding Ref.1 Traceable author statement. Source: ProtInc selenide, water dikinase activity Inferred from electronic annotation. Source: InterPro selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Selenide, water dikinase 1		PRO_0000127648
Regions
	Nucleotide binding	268 – 274	7	ATP Potential
Sites
	Active site	31	1	Potential
	Site	32	1	Important for catalytic activity By similarity
Experimental info
	Mutagenesis	268	1	G → C: No change in ATP-binding
	Mutagenesis	270	1	G → R: No change in ATP-binding
	Mutagenesis	273	1	G → A, D or V: Loss of ATP-binding
	Mutagenesis	274	1	H → N: Reduced ATP-binding
	Mutagenesis	274	1	H → Y: Increased ATP-binding
	Sequence conflict	260	1	A → T in AAA87567. Ref.1
	Sequence conflict	377 – 392	16	PQVAT…PGATS → HKWPLKT in AAA87567. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49903-1 [UniParc]. Last modified December 13, 2002. Version 2. Checksum: E9636E38146D926D	FASTA	392	42,911
	10 20 30 40 50 60 MSTRESFNPE SYELDKSFRL TRFTELKGTG CKVPQDVLQK LLESLQENHF QEDEQFLGAV 70 80 90 100 110 120 MPRLGIGMDT CVIPLRHGGL SLVQTTDYIY PIVDDPYMMG RIACANVLSD LYAMGVTECD 130 140 150 160 170 180 NMLMLLGVSN KMTDRERDKV MPLIIQGFKD AAEEAGTSVT GGQTVLNPWI VLGGVATTVC 190 200 210 220 230 240 QPNEFIMPDN AVPGDVLVLT KPLGTQVAVA VHQWLDIPEK WNKIKLVVTQ EDVELAYQEA 250 260 270 280 290 300 MMNMARLNRT AAGLMHTFNA HAATDITGFG ILGHAQNLAK QQRNEVSFVI HNLPVLAKMA 310 320 330 340 350 360 AVSKACGNMF GLMHGTCPET SGGLLICLPR EQAARFCAEI KSPKYGEGHQ AWIIGIVEKG 370 380 390 NRTARIIDKP RIIEVAPQVA TQNVNPTPGA TS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and functional characterization of human selenophosphate synthetase, an essential component of selenoprotein synthesis." Low S.C., Harney J.W., Berry M.J. J. Biol. Chem. 270:21659-21664(1995) [PubMed: 7665581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ATP-BINDING, MUTAGENESIS OF GLY-268; GLY-270; GLY-273 AND HIS-274. Tissue: Liver.
[2]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta and PNS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U34044 mRNA. Translation: AAA87567.1. AL138764, AL355870 Genomic DNA. Translation: CAI12907.1. AL355870, AL138764 Genomic DNA. Translation: CAI14198.1. CH471072 Genomic DNA. Translation: EAW86290.1. BC000941 mRNA. Translation: AAH00941.1. BC063816 mRNA. Translation: AAH63816.1.
RefSeq	NP_036379.2.
UniGene	Hs.124027
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P49903.
Proteomic databases
PeptideAtlas	P49903.
Genome annotation databases
Ensembl	ENSG00000086475. Homo sapiens. [Contig view]
GeneID	22929.
KEGG	hsa:22929.
Organism-specific databases
H-InvDB	HIX0008655.
HGNC	HGNC:19685. SEPHS1.
MIM	600902. gene.
PharmGKB	PA134905215.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P49903.
HOVERGEN	P49903.
Gene expression databases
ArrayExpress	P49903.
CleanEx	HS_SEPHS1.
GermOnline	ENSG00000086475. Homo sapiens.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004536. SelD. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
PIRSF	PIRSF036407. Selenphspht_syn. 1 hit.
TIGRFAMs	TIGR00476. selD. 1 hit.
ProtoNet	Search...
Other Resources
LinkHub	P49903.
NextBio	43651.
SOURCE	Search...

Entry information

Entry name

SPS1_HUMAN

Accession

Primary (citable) accession number: P49903
Secondary accession number(s): Q5T5U8, Q9BVT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 13, 2002
Last modified:	November 25, 2008
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)