P49894 (IOD1_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type I iodothyronine deiodinase
EC=1.97.1.10

Alternative name(s):
5DI
DIOI
Type 1 DI
Type-I 5'-deiodinase

Gene names

Name:	DIO1
Synonyms:	ITDI1, TXDI1

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine). Plays a role in providing a source of plasma T3 by deiodination of T4 in peripheral tissues such as liver and kidney.
Catalytic activity	3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the iodothyronine deiodinase family.

Ontologies

Keywords
Biological process	Thyroid hormones biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Selenocysteine
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	thyroxine 5'-deiodinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Type I iodothyronine deiodinase		PRO_0000154310
Regions
	Transmembrane	13 – 33	21	Helical; Potential
Sites
	Active site	121	1
Amino acid modifications
	Non-standard residue	121	1	Selenocysteine

Sequences

	Sequence		Length	Mass (Da)
	P49894 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: E347D761A60618DC	FASTA	244	28,424
	10 20 30 40 50 60 MGLPRPVLWL RRLWVLLQVA VQVAVGKVFL KLFPARVKQH IVAMNGKNPH FSYDNWAPTL 70 80 90 100 110 120 YSMQYFWFVL KVQWQRLEDR TEPGGLAPNC PVVRLSGQRC NIWDFMQGNR PLVLNFGSCT 130 140 150 160 170 180 UPSFLFKFDQ FKRLIEDFCS TADFLIIYIE EAHASDGWAF KNNVNIRTHQ TLQDRLQAAR 190 200 210 220 230 240 LLLDRAPPCP VVVDTMRNQS SQFYAALPER LFVLQEGRIL YKGKPGPWNY HPEEVRAVLE KLHS

References

[1]

"Identification of critical amino acids for 3,5,3'-triiodothyronine deiodination by human type 1 deiodinase based on comparative functional-structural analyses of the human, dog, and rat enzymes." Toyoda N., Harney J.W., Berry M.J., Larsen P.R. J. Biol. Chem. 269:20329-20334(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Mongrel. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U11762 mRNA. Translation: AAA53207.2.
RefSeq	NP_001007127.1. NM_001007126.2.
UniGene	Cfa.3500.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	403635.
KEGG	cfa:403635.
Organism-specific databases
CTD	1733.
Phylogenomic databases
eggNOG	NOG149534.
HOGENOM	HOG000169547.
HOVERGEN	HBG000099.
InParanoid	P49894.
KO	K01562.
OrthoDB	EOG40K80M.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR000643. Iodothyronine_deiodinase. IPR008261. Iodothyronine_deiodinase_AS. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR11781. PTHR11781. 1 hit.
Pfam	PF00837. T4_deiodinase. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS01205. T4_DEIODINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20817140.

Entry information

Entry name

IOD1_CANFA

Accession

Primary (citable) accession number: P49894

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	February 26, 2008
Last modified:	April 3, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program