Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein Wnt-11b

Gene

wnt11b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand for the frizzled7 transmembrane receptor. Primarily acts via non-canonical Wnt pathways mediated by either Ca2+ and PKC, or by JNK and dvl2/dsh. Depending on the cellular context, can also signal via the canonical Wnt pathway mediated by beta-catenin and dvl2/dsh. May also inhibit canonical Wnt signaling. Maternally initiates dorsal/ventral axis formation by a canonical route, which signals via lrp6. In a complex with wnt5a, activates the canonical and non-canonical processes involved in axis formation. In the non-canonical pathway, acts through fzd7/fz7 to induce phosphorylation of dvl2/dsh. Signals through a non-canonical Wnt pathway to regulate convergent extension movements during gastrulation. Interactions with the secreted Wnt antagonist sfrp5 to coordinate foregut development, acting via a non-canonical Wnt pathway whereby sfrp5 restricts wnt11b activity to prevent inappropriate foregut formation. Mediates cardiogenesis via non-canonical Wnt signaling involving JNK-activation and PKC. Acts redundantly with wnt11/wnt11r during pronephros induction.21 Publications

Miscellaneous

Xenopus and other lower vertebrates contain duplicated wnt11 genes resulting from an ancient gene duplication event, but the second copy has since been lost in mammals. This gene was originally called wnt-11 (PubMed:8306880) but was renamed to wnt11b (PubMed:17436276) after the discovery of wnt11r (now called wnt11).2 Publications

GO - Molecular functioni

  • frizzled binding Source: UniProtKB
  • growth factor binding Source: UniProtKB
  • GTPase activator activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • protein kinase activator activity Source: UniProtKB
  • transcription regulatory region DNA binding Source: UniProtKB
  • Wnt-protein binding Source: UniProtKB

GO - Biological processi

  • canonical Wnt signaling pathway Source: UniProtKB
  • cell migration involved in gastrulation Source: UniProtKB
  • convergent extension involved in gastrulation Source: UniProtKB
  • dorsal/ventral axis specification Source: UniProtKB
  • endocytosis Source: UniProtKB
  • heart development Source: UniProtKB
  • heart formation Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of canonical Wnt signaling pathway Source: UniProtKB
  • negative regulation of cell death Source: UniProtKB
  • negative regulation of cell growth Source: UniProtKB
  • negative regulation of cell migration Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • neural crest cell migration Source: UniProtKB
  • neuroendocrine cell differentiation Source: UniProtKB
  • non-canonical Wnt signaling pathway Source: UniProtKB
  • palate development Source: UniProtKB
  • positive regulation of cell migration Source: UniProtKB
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of GTPase activity Source: UniProtKB
  • positive regulation of JNK cascade Source: UniProtKB
  • positive regulation of protein kinase C signaling Source: UniProtKB
  • positive regulation of stress fiber assembly Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • pronephros development Source: UniProtKB
  • protein localization to cell surface Source: UniProtKB
  • protein phosphorylation Source: UniProtKB
  • Wnt signaling pathway Source: UniProtKB
  • Wnt signaling pathway, planar cell polarity pathway Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein
Biological processGastrulation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-11b
Alternative name(s):
Protein Wnt-11
Short name:
XWnt-11
Gene namesi
Name:wnt11b
Synonyms:wnt-11, wnt11
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6079153. wnt11b.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi88N → Q: Loss of glycosylation. Prevents secretion. No effect on wnt5a-binding. 1 Publication1
Mutagenesisi274Y → F: Loss of sulfation. No effect on secretion or homodimerization but severely reduces interaction with wnt5a and canonical Wnt signaling activity; when associated with F-281. 1 Publication1
Mutagenesisi281Y → F: Loss of sulfation. No effect on secretion or homodimerization but severely reduces interaction with wnt5a and canonical Wnt signaling activity; when associated with F-274. 1 Publication1
Mutagenesisi299N → Q: Loss of glycosylation. Prevents secretion. No effect on wnt5a-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000004147023 – 353Protein Wnt-11bAdd BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi31N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi78 ↔ 89By similarity
Glycosylationi88N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi128 ↔ 136By similarity
Disulfide bondi138 ↔ 155By similarity
Disulfide bondi208 ↔ 222By similarity
Disulfide bondi210 ↔ 217By similarity
Lipidationi214O-palmitoleoyl serine; by PORCNBy similarity1
Modified residuei274Sulfotyrosine1 Publication1
Modified residuei281Sulfotyrosine1 Publication1
Disulfide bondi298 ↔ 313By similarity
Glycosylationi299N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi328 ↔ 343By similarity
Disulfide bondi330 ↔ 340By similarity
Disulfide bondi335 ↔ 336By similarity

Post-translational modificationi

Glycosylation is required for protein secretion.1 Publication
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Sulfation

PTM databases

iPTMnetiP49893.

Expressioni

Tissue specificityi

Transcripts are expressed ubiquitously in early oocytes but become vegetally localized during mid-oogenesis then enriched on the dorsal side by the 8 to 16 cell stage. The protein becomes asymmetrically concentrated on the dorsal side by the 64-cell stage. During gastrulation, expressed in the lateral and ventral marginal zone, and during tadpole stages in the somites and first branchial arch. Weakly expressed in the pronephros from at least stage 12.5, with kidney expression increasing until stage 35. Expressed in the prospective posterior gut between stages 13 and 20, and in the deep foregut endoderm. Prior to neural crest cell migration, expressed in a domain flanking the neural crest on the lateral or epidermal side (the opposite side to wnt11/wnt11-r).14 Publications

Developmental stagei

Expressed both maternally and zygotically in oocytes and embryos through to swimming tadpole stages.1 Publication

Inductioni

By t/xbra. By gata4 and gata6. Repressed by zbtb33/kaiso.5 Publications

Interactioni

Subunit structurei

Homodimer. Secreted homodimers form a complex with wnt5a homodimers; tyrosine sulfation of both wnt11 and wnt5a by tpst1 is required for this interaction. Interacts with the transmembrane receptor fzd7/fz7. Interacts with lrp6 and ryk. Interacts with tdgf1/frl1. Interacts weakly with frzb1 and strongly with frzb2/crescent. Interaction with frzb2/crescent antagonizes wnt11 function in the neuroectoderm, but enhances it in mesodermal tissue.7 Publications

GO - Molecular functioni

  • frizzled binding Source: UniProtKB
  • growth factor binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • Wnt-protein binding Source: UniProtKB

Protein-protein interaction databases

IntActiP49893. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliP49893.
SMRiP49893.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG001595.
KOiK01384.

Family and domain databases

InterProiView protein in InterPro
IPR005817. Wnt.
IPR018161. Wnt_CS.
PANTHERiPTHR12027. PTHR12027. 1 hit.
PfamiView protein in Pfam
PF00110. wnt. 1 hit.
PRINTSiPR01349. WNTPROTEIN.
SMARTiView protein in SMART
SM00097. WNT1. 1 hit.
PROSITEiView protein in PROSITE
PS00246. WNT1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49893-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPTRHWVTP LLLLCCSGIC GAIQWLGLTV NGSRVAWNES EHCRLLDGLV
60 70 80 90 100
PDQSQLCKRN LELMQSVVNA AKQTKLTCQM TLSDMRWNCS SVENAPSFTP
110 120 130 140 150
DLSKGTRESA FVYALASATL SHTIARACAS GELPTCSCGA TPAEVPGTGF
160 170 180 190 200
RWGGCGDNLH YGLNMGSAFV DAPMKSSKSA GTQATKIMNL HNNAVGRQVL
210 220 230 240 250
MDSLETKCKC HGVSGSCSVK TCWKGLQDLP HIANELKSKY LGATKVIHRQ
260 270 280 290 300
TGTRRQLVPR ELDIRPVRES ELVYLVSSPD YCTKNPKLGS YGTQDRLCNK
310 320 330 340 350
TSVGSDSCNL MCCGRGYNAY TETIVERCQC KYHWCCYVMC KKCERTVERY

VCK
Length:353
Mass (Da):38,785
Last modified:March 2, 2010 - v2
Checksum:iB59609AB88915554
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti257L → H in AAA19697 (PubMed:8306880).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23542 mRNA. Translation: AAA19697.1.
BC084745 mRNA. Translation: AAH84745.1.
PIRiI51572.
RefSeqiNP_001084327.1. NM_001090858.1.
UniGeneiXl.24008.

Genome annotation databases

GeneIDi399441.
KEGGixla:399441.

Similar proteinsi

Entry informationi

Entry nameiWN11B_XENLA
AccessioniPrimary (citable) accession number: P49893
Secondary accession number(s): Q5U5D9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 2, 2010
Last modified: October 25, 2017
This is version 86 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

PubMed:11159911 show a lack of role in heart induction but PubMed:12167861 show a role in heart formation; the discrepancy may be due to duplication of Xenopus wnt11 genes.Curated

Documents

  1. SIMILARITY comments
    Index of protein domains and families