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Protein

Estrogen sulfotransferase, testis isoform

Gene

Sult1e1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + estrone = adenosine 3',5'-bisphosphate + estrone 3-sulfate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei108Proton acceptor1 Publication1
Binding sitei130PAPS1
Binding sitei138PAPS1
Binding sitei193PAPS1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 53PAPS6
Nucleotide bindingi227 – 232PAPS6
Nucleotide bindingi257 – 259PAPS3

GO - Molecular functioni

GO - Biological processi

  • estrogen metabolic process Source: UniProtKB
  • female pregnancy Source: MGI
  • positive regulation of fat cell differentiation Source: MGI
  • sulfation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Lipid-binding, Steroid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Estrogen sulfotransferase, testis isoform (EC:2.8.2.4)
Alternative name(s):
Sulfotransferase 1E1
Short name:
ST1E1
Sulfotransferase, estrogen-preferring
Gene namesi
Name:Sult1e1
Synonyms:Ste
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:98431. Sult1e1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi48K → M: Loss of enzyme activity. 1 Publication1
Mutagenesisi48K → R: Reduced enzyme activity. 1 Publication1
Mutagenesisi106K → A: Strongly reduced enzyme activity. Strongly reduced affinity for estradiol. 1 Publication1
Mutagenesisi106K → R or S: Strongly reduced enzyme activity. Strongly reduced affinity for estradiol. 1 Publication1
Mutagenesisi108H → K, L, S or R: Loss of enzyme activity. 1 Publication1
Mutagenesisi240Y → F: Slightly decreased enzyme activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3052.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000851541 – 295Estrogen sulfotransferase, testis isoformAdd BLAST295

Proteomic databases

PaxDbiP49891.
PRIDEiP49891.

PTM databases

PhosphoSitePlusiP49891.

Expressioni

Tissue specificityi

Testis and at very low level in the placenta.

Gene expression databases

CleanExiMM_SULT1E1.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP49891. 1 interactor.
STRINGi10090.ENSMUSP00000031201.

Chemistry databases

BindingDBiP49891.

Structurei

Secondary structure

1295
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 11Combined sources4
Beta strandi12 – 15Combined sources4
Beta strandi18 – 21Combined sources4
Helixi22 – 24Combined sources3
Turni25 – 27Combined sources3
Helixi28 – 32Combined sources5
Beta strandi41 – 45Combined sources5
Helixi51 – 62Combined sources12
Turni63 – 65Combined sources3
Helixi75 – 78Combined sources4
Turni87 – 89Combined sources3
Helixi92 – 97Combined sources6
Beta strandi104 – 107Combined sources4
Helixi111 – 113Combined sources3
Helixi116 – 120Combined sources5
Beta strandi124 – 129Combined sources6
Helixi132 – 145Combined sources14
Helixi155 – 164Combined sources10
Helixi172 – 182Combined sources11
Beta strandi188 – 192Combined sources5
Helixi193 – 198Combined sources6
Helixi200 – 210Combined sources11
Helixi217 – 226Combined sources10
Helixi229 – 234Combined sources6
Turni236 – 242Combined sources7
Turni245 – 247Combined sources3
Turni250 – 252Combined sources3
Helixi263 – 266Combined sources4
Helixi270 – 284Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AQUX-ray1.60A/B1-295[»]
1AQYX-ray1.75A/B1-295[»]
1BO6X-ray2.10A/B1-295[»]
ProteinModelPortaliP49891.
SMRiP49891.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49891.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni106 – 108Substrate binding3

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Phylogenomic databases

eggNOGiKOG1584. Eukaryota.
ENOG4111H56. LUCA.
HOGENOMiHOG000037209.
HOVERGENiHBG001195.
InParanoidiP49891.
PhylomeDBiP49891.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P49891-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METSMPEYYE VFGEFRGVLM DKRFTKYWED VEMFLARPDD LVIATYPKSG
60 70 80 90 100
TTWISEVVYM IYKEGDVEKC KEDAIFNRIP YLECRNEDLI NGIKQLKEKE
110 120 130 140 150
SPRIVKTHLP PKLLPASFWE KNCKMIYLCR NAKDVAVSYY YFLLMITSYP
160 170 180 190 200
NPKSFSEFVE KFMQGQVPYG SWYDHVKAWW EKSKNSRVLF MFYEDMKEDI
210 220 230 240 250
RREVVKLIEF LERKPSAELV DRIIQHTSFQ EMKNNPSTNY TMMPEEMMNQ
260 270 280 290
KVSPFMRKGI IGDWKNHFPE ALRERFDEHY KQQMKDCTVK FRMEL
Length:295
Mass (Da):35,590
Last modified:July 15, 1999 - v2
Checksum:i8E85AB47952BFB1C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113L → V in AAB34320 (PubMed:7750469).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78182 mRNA. Translation: AAB34320.1.
CCDSiCCDS19392.1.
PIRiI53296.
UniGeneiMm.89655.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78182 mRNA. Translation: AAB34320.1.
CCDSiCCDS19392.1.
PIRiI53296.
UniGeneiMm.89655.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AQUX-ray1.60A/B1-295[»]
1AQYX-ray1.75A/B1-295[»]
1BO6X-ray2.10A/B1-295[»]
ProteinModelPortaliP49891.
SMRiP49891.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP49891. 1 interactor.
STRINGi10090.ENSMUSP00000031201.

Chemistry databases

BindingDBiP49891.
ChEMBLiCHEMBL3052.

PTM databases

PhosphoSitePlusiP49891.

Proteomic databases

PaxDbiP49891.
PRIDEiP49891.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:98431. Sult1e1.

Phylogenomic databases

eggNOGiKOG1584. Eukaryota.
ENOG4111H56. LUCA.
HOGENOMiHOG000037209.
HOVERGENiHBG001195.
InParanoidiP49891.
PhylomeDBiP49891.

Miscellaneous databases

EvolutionaryTraceiP49891.
PROiP49891.
SOURCEiSearch...

Gene expression databases

CleanExiMM_SULT1E1.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiST1E1_MOUSE
AccessioniPrimary (citable) accession number: P49891
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 15, 1999
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Abnormal high expression in liver in obese and diabetogenic C57BL/KSJ-DB/DB strain mice. Female > male. Normal level in liver.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.