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Protein

Estrogen sulfotransferase

Gene

SULT1E1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + estrone = adenosine 3',5'-bisphosphate + estrone 3-sulfate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei107Proton acceptorBy similarity1
Binding sitei129PAPS1
Binding sitei137PAPS1
Binding sitei192PAPS1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 52PAPS6
Nucleotide bindingi226 – 231PAPS6
Nucleotide bindingi256 – 258PAPS3

GO - Molecular functioni

  • aryl sulfotransferase activity Source: Reactome
  • estrone sulfotransferase activity Source: Reactome
  • flavonol 3-sulfotransferase activity Source: BHF-UCL
  • steroid binding Source: UniProtKB-KW
  • steroid sulfotransferase activity Source: UniProtKB
  • sulfotransferase activity Source: MGI

GO - Biological processi

  • 3'-phosphoadenosine 5'-phosphosulfate metabolic process Source: Reactome
  • estrogen metabolic process Source: UniProtKB
  • positive regulation of fat cell differentiation Source: CACAO
  • steroid metabolic process Source: ProtInc
  • sulfation Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Lipid-binding, Steroid-binding

Enzyme and pathway databases

BioCyciZFISH:HS03210-MONOMER.
BRENDAi2.8.2.4. 2681.
ReactomeiR-HSA-156584. Cytosolic sulfonation of small molecules.

Names & Taxonomyi

Protein namesi
Recommended name:
Estrogen sulfotransferase (EC:2.8.2.4)
Alternative name(s):
EST-1
Sulfotransferase 1E1
Short name:
ST1E1
Sulfotransferase, estrogen-preferring
Gene namesi
Name:SULT1E1
Synonyms:STE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:11377. SULT1E1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi137S → A: Decreased gradually the catalytic activity. 1 Publication1
Mutagenesisi137S → C: Decreased gradually the catalytic activity. 1 Publication1
Mutagenesisi269V → E: Does not prevent the formation of homodimer. 1

Organism-specific databases

DisGeNETi6783.
OpenTargetsiENSG00000109193.
PharmGKBiPA340.

Chemistry databases

ChEMBLiCHEMBL2346.
DrugBankiDB00316. Acetaminophen.
DB01176. Cyclizine.

Polymorphism and mutation databases

BioMutaiSULT1E1.
DMDMi1711604.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000851531 – 294Estrogen sulfotransferaseAdd BLAST294

Proteomic databases

PaxDbiP49888.
PeptideAtlasiP49888.
PRIDEiP49888.

PTM databases

iPTMnetiP49888.
PhosphoSitePlusiP49888.

Expressioni

Tissue specificityi

Liver, intestine and at lower level in the kidney.

Gene expression databases

BgeeiENSG00000109193.
CleanExiHS_SULT1E1.
ExpressionAtlasiP49888. baseline and differential.
GenevisibleiP49888. HS.

Organism-specific databases

HPAiCAB047344.
HPA028213.
HPA028728.

Interactioni

Subunit structurei

Homodimer.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PDE9AO760833EBI-712512,EBI-742764

Protein-protein interaction databases

BioGridi112660. 14 interactors.
IntActiP49888. 12 interactors.
MINTiMINT-1368473.
STRINGi9606.ENSP00000226444.

Chemistry databases

BindingDBiP49888.

Structurei

Secondary structure

1294
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 10Combined sources7
Beta strandi11 – 14Combined sources4
Beta strandi17 – 20Combined sources4
Helixi21 – 25Combined sources5
Helixi27 – 31Combined sources5
Beta strandi40 – 45Combined sources6
Helixi50 – 61Combined sources12
Turni62 – 64Combined sources3
Helixi66 – 69Combined sources4
Beta strandi70 – 72Combined sources3
Helixi74 – 77Combined sources4
Turni86 – 88Combined sources3
Helixi91 – 96Combined sources6
Beta strandi103 – 106Combined sources4
Helixi110 – 112Combined sources3
Helixi115 – 119Combined sources5
Beta strandi123 – 128Combined sources6
Helixi131 – 144Combined sources14
Helixi154 – 162Combined sources9
Helixi171 – 181Combined sources11
Beta strandi187 – 191Combined sources5
Helixi192 – 197Combined sources6
Helixi199 – 209Combined sources11
Helixi216 – 225Combined sources10
Helixi228 – 233Combined sources6
Turni235 – 237Combined sources3
Turni244 – 246Combined sources3
Turni249 – 251Combined sources3
Helixi262 – 265Combined sources4
Helixi269 – 283Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G3MX-ray1.70A/B1-294[»]
1HY3X-ray1.80A/B1-294[»]
4JVLX-ray1.94A/B1-294[»]
4JVMX-ray1.99A/B1-294[»]
4JVNX-ray2.05A/B1-294[»]
ProteinModelPortaliP49888.
SMRiP49888.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49888.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni105 – 107Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Phylogenomic databases

eggNOGiKOG1584. Eukaryota.
ENOG4111H56. LUCA.
GeneTreeiENSGT00760000118932.
HOGENOMiHOG000037209.
HOVERGENiHBG001195.
InParanoidiP49888.
KOiK01016.
OMAiQMKESTL.
OrthoDBiEOG091G0D5F.
PhylomeDBiP49888.
TreeFamiTF321745.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P49888-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSELDYYEK FEEVHGILMY KDFVKYWDNV EAFQARPDDL VIATYPKSGT
60 70 80 90 100
TWVSEIVYMI YKEGDVEKCK EDVIFNRIPF LECRKENLMN GVKQLDEMNS
110 120 130 140 150
PRIVKTHLPP ELLPASFWEK DCKIIYLCRN AKDVAVSFYY FFLMVAGHPN
160 170 180 190 200
PGSFPEFVEK FMQGQVPYGS WYKHVKSWWE KGKSPRVLFL FYEDLKEDIR
210 220 230 240 250
KEVIKLIHFL ERKPSEELVD RIIHHTSFQE MKNNPSTNYT TLPDEIMNQK
260 270 280 290
LSPFMRKGIT GDWKNHFTVA LNEKFDKHYE QQMKESTLKF RTEI
Length:294
Mass (Da):35,126
Last modified:October 1, 1996 - v1
Checksum:i9EC8923D20757D57
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti154F → L in AAH27956 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01890722D → Y.1 PublicationCorresponds to variant rs11569705dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08098 mRNA. Translation: AAA82125.1.
U20521
, U20515, U20516, U20517, U20518, U20519, U20520 Genomic DNA. Translation: AAC50286.1.
S77383 mRNA. Translation: AAB34601.1.
Y11195 mRNA. Translation: CAA72079.1.
AY436634 Genomic DNA. Translation: AAQ97179.1.
BC027956 mRNA. Translation: AAH27956.1.
U55764 mRNA. Translation: AAB51658.1.
CCDSiCCDS3531.1.
PIRiJC2229.
RefSeqiNP_005411.1. NM_005420.2.
UniGeneiHs.479898.

Genome annotation databases

EnsembliENST00000226444; ENSP00000226444; ENSG00000109193.
GeneIDi6783.
KEGGihsa:6783.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08098 mRNA. Translation: AAA82125.1.
U20521
, U20515, U20516, U20517, U20518, U20519, U20520 Genomic DNA. Translation: AAC50286.1.
S77383 mRNA. Translation: AAB34601.1.
Y11195 mRNA. Translation: CAA72079.1.
AY436634 Genomic DNA. Translation: AAQ97179.1.
BC027956 mRNA. Translation: AAH27956.1.
U55764 mRNA. Translation: AAB51658.1.
CCDSiCCDS3531.1.
PIRiJC2229.
RefSeqiNP_005411.1. NM_005420.2.
UniGeneiHs.479898.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G3MX-ray1.70A/B1-294[»]
1HY3X-ray1.80A/B1-294[»]
4JVLX-ray1.94A/B1-294[»]
4JVMX-ray1.99A/B1-294[»]
4JVNX-ray2.05A/B1-294[»]
ProteinModelPortaliP49888.
SMRiP49888.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112660. 14 interactors.
IntActiP49888. 12 interactors.
MINTiMINT-1368473.
STRINGi9606.ENSP00000226444.

Chemistry databases

BindingDBiP49888.
ChEMBLiCHEMBL2346.
DrugBankiDB00316. Acetaminophen.
DB01176. Cyclizine.

PTM databases

iPTMnetiP49888.
PhosphoSitePlusiP49888.

Polymorphism and mutation databases

BioMutaiSULT1E1.
DMDMi1711604.

Proteomic databases

PaxDbiP49888.
PeptideAtlasiP49888.
PRIDEiP49888.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000226444; ENSP00000226444; ENSG00000109193.
GeneIDi6783.
KEGGihsa:6783.

Organism-specific databases

CTDi6783.
DisGeNETi6783.
GeneCardsiSULT1E1.
HGNCiHGNC:11377. SULT1E1.
HPAiCAB047344.
HPA028213.
HPA028728.
MIMi600043. gene.
neXtProtiNX_P49888.
OpenTargetsiENSG00000109193.
PharmGKBiPA340.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1584. Eukaryota.
ENOG4111H56. LUCA.
GeneTreeiENSGT00760000118932.
HOGENOMiHOG000037209.
HOVERGENiHBG001195.
InParanoidiP49888.
KOiK01016.
OMAiQMKESTL.
OrthoDBiEOG091G0D5F.
PhylomeDBiP49888.
TreeFamiTF321745.

Enzyme and pathway databases

BioCyciZFISH:HS03210-MONOMER.
BRENDAi2.8.2.4. 2681.
ReactomeiR-HSA-156584. Cytosolic sulfonation of small molecules.

Miscellaneous databases

EvolutionaryTraceiP49888.
GeneWikiiSULT1E1.
GenomeRNAii6783.
PROiP49888.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000109193.
CleanExiHS_SULT1E1.
ExpressionAtlasiP49888. baseline and differential.
GenevisibleiP49888. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiST1E1_HUMAN
AccessioniPrimary (citable) accession number: P49888
Secondary accession number(s): Q8N6X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.