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Protein

Estrogen receptor

Gene

ESR1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi186 – 25166Nuclear receptorPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri186 – 20621NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri222 – 24625NR C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Lipid-binding, Metal-binding, Steroid-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Estrogen receptor
Short name:
ER
Alternative name(s):
ER-alpha
Estradiol receptor
Nuclear receptor subfamily 3 group A member 1
Gene namesi
Name:ESR1
Synonyms:ESR, NR3A1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation
  • Cytoplasm By similarity
  • Golgi apparatus By similarity
  • Cell membrane By similarity

  • Note: Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2095170.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 596596Estrogen receptorPRO_0000053616Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi10 – 101O-linked (GlcNAc)By similarity
Modified residuei104 – 1041Phosphoserine; by CDK2By similarity
Modified residuei106 – 1061Phosphoserine; by CDK2By similarity
Modified residuei119 – 1191PhosphoserineBy similarity
Modified residuei168 – 1681Phosphoserine; by CK2By similarity
Modified residuei261 – 2611Asymmetric dimethylarginine; by PRMT1By similarity
Lipidationi448 – 4481S-palmitoyl cysteineBy similarity
Modified residuei538 – 5381Phosphotyrosine; by Tyr-kinasesBy similarity
Glycosylationi572 – 5721O-linked (GlcNAc)By similarity

Post-translational modificationi

Glycosylated; contains N-acetylglucosamine, probably O-linked.By similarity
Ubiquitinated. Deubiquitinated by OTUB1 (By similarity).By similarity
Dimethylated by PRMT1 at Arg-261. The methylation may favor cytoplasmic localization (By similarity).By similarity
Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Dephosphorylation at Ser-119 by PPP5C inhibits its transactivation activity (By similarity). Phosphorylated by LMTK3 (in vitro) (By similarity).By similarity
Palmitoylated at Cys-448 by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation, but not for signaling mediated by the nuclear hormone receptor (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Methylation, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP49884.
PRIDEiP49884.

PTM databases

UniCarbKBiP49884.

Interactioni

Subunit structurei

Binds DNA as a homodimer (By similarity). Can form a heterodimer with ESR2 (By similarity). Interacts with coactivator NCOA5. Interacts with NCOA7; the interaction is ligand-inducible. Interacts with AKAP13, CUEDC2, HEXIM1, KDM5A, MAP1S, PELP1, SMARD1, and UBE1C. Interacts with MUC1; the interaction is stimulated by 7 beta-estradiol (E2) and enhances ERS1-mediated transcription. Interacts with DNTTIP2, and UIMC1. Interacts with KMT2D/MLL2. Interacts with ATAD2; the interaction is enhanced by estradiol. Interacts with KIF18A and LDB1. Interacts with RLIM (via its C-terminus). Interacts with MACROD1. Interacts with SH2D4A and PLCG. Interacts with SH2D4A; the interaction blocks binding to PLCG and inhibits estrogen-induced cell proliferation. Interacts with DYNLL1. Interacts with CCDC62; the interaction requires estradiol and appears to enhance the transcription of target genes. Interacts with NR2C1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with DYX1C1. Interacts with PRMT2. Interacts with PI3KR1 or PIK3R2, SRC and PTK2/FAK1. Interacts with RBFOX2. Interacts with EP300; the interaction is estrogen-dependent and enhanced by CITED1. Interacts with CITED1; the interaction is estrogen-dependent (By similarity). Interacts with FAM120B, FOXL2, PHB2 and SLC30A9. Interacts with coactivators NCOA3 and NCOA6. Interacts with STK3/MST2 only in the presence of SAV1 and vice-versa. Binds to CSNK1D. Interacts with NCOA2; NCOA2 can interact with ESR1 AF-1 and AF-2 domains simultaneously and mediate their transcriptional synergy. Interacts with DDX5. Interacts with NCOA1; the interaction seems to require a self-association of N-terminal and C-terminal regions. Interacts with ZNF366, DDX17, NFKB1, RELA, SP1 and SP3. Interacts with NRIP1. Interacts with GPER1; the interaction occurs in an estrogen-dependent manner. Interacts with CLOCK and the interaction is stimulated by estrogen (By similarity). Interacts with TRIP4 (ufmylated); estrogen dependent (By similarity). Interacts with LMTK3; the interaction phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. Interacts with CCAR2 (via N-terminus) in a ligand-independent manner. Interacts with ZFHX3 (By similarity). Interacts with SFR1 in a ligand-dependent and -independent manner (By similarity).By similarity

Protein-protein interaction databases

BioGridi160595. 1 interaction.
STRINGi9913.ENSBTAP00000009422.

Chemistry

BindingDBiP49884.

Structurei

3D structure databases

ProteinModelPortaliP49884.
SMRiP49884. Positions 181-254, 302-551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 185185Modulating (transactivation AF-1); mediates interaction with MACROD1By similarityAdd
BLAST
Regioni36 – 175140Interaction with DDX5; self-associationBy similarityAdd
BLAST
Regioni36 – 4813Required for interaction with NCOA1By similarityAdd
BLAST
Regioni186 – 311126Mediates interaction with DNTTIP2By similarityAdd
BLAST
Regioni252 – 31160HingeAdd
BLAST
Regioni263 – 596334Interaction with AKAP13By similarityAdd
BLAST
Regioni265 – 595331Self-associationBy similarityAdd
BLAST
Regioni312 – 595284Transactivation AF-2By similarityAdd
BLAST
Regioni312 – 552241Steroid-bindingAdd
BLAST

Domaini

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. The modulating domain, also known as A/B or AF-1 domain has a ligand-independent transactivation function. The C-terminus contains a ligand-dependent transactivation domain, also known as E/F or AF-2 domain which overlaps with the ligand binding domain. AF-1 and AF-2 activate transcription independently and synergistically and act in a promoter- and cell-specific manner (By similarity).By similarity

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri186 – 20621NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri222 – 24625NR C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
HOGENOMiHOG000233468.
HOVERGENiHBG108344.
InParanoidiP49884.
KOiK08550.

Family and domain databases

Gene3Di1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProiIPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR024178. Oest_rcpt/oest-rel_rcp.
IPR001292. Oestr_rcpt.
IPR024736. Oestrogen-typ_rcpt_final_C_dom.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF12743. ESR1_C. 1 hit.
PF00104. Hormone_recep. 1 hit.
PF02159. Oest_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PIRSFiPIRSF500101. ER-a. 1 hit.
PIRSF002527. ER-like_NR. 1 hit.
PRINTSiPR00543. OESTROGENR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49884-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMTLHTKAS GMALLHQIQA NELEPLNRPQ LKIPLERPLG EVYMDSSKPA
60 70 80 90 100
VYNYPEGAAY DFNAAAPASA PVYGQSGLPY GPGSEAAAFG ANGLGAFPPL
110 120 130 140 150
NSVSPSPLVL LHPPPQPLSP FLHPHGQQVP YYLENESSGY AVREAGPPAY
160 170 180 190 200
YRPNSDNRRQ GGRERLASTS DKGSMAMESA KETRYCAVCN DYASGYHYGV
210 220 230 240 250
WSCEGCKAFF KRSIQGHNDY MCPATNQCTI DKNRRKSCQA CRLRKCYEVG
260 270 280 290 300
MMKGGIRKDR RGGRMLKHKR QRDDGEGRNE AVPSGDMRAA NLWPSPIMIK
310 320 330 340 350
HTKKNSPVLS LTADQMISAL LEAEPPIIYS EYDPTRPFSE ASMMGLLTNL
360 370 380 390 400
ADRELVHMIN WAKRVPGFVD LALHDQVHLL ECAWLEILMI GLVWRSMEHP
410 420 430 440 450
GKLLFAPNLL LDRNQGKCVE GMVEIFDMLL ATSSRFRMMN LQGEEFVCLK
460 470 480 490 500
SIILLNSGVY TFLSSTLRSL EEKDHIHRVL DKITDTLIHL MAKAGLTLQQ
510 520 530 540 550
QHRRLAQLLL ILSHFRHMSN KGMEHLYSMK CKNVVPLYDL LLEMLDAHRL
560 570 580 590
HAPANFGSAP PEDVNQSQLA PTGCTSSHSL QTYYITGEAE NFPSTV
Length:596
Mass (Da):66,489
Last modified:November 9, 2004 - v3
Checksum:i3E2CE85BE7844FF2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti372 – 3721A → T in CAA47317 (PubMed:7555698).Curated
Sequence conflicti386 – 3861E → A in AAB09548 (Ref. 3) Curated
Sequence conflicti401 – 4011G → V in CAA47317 (PubMed:7555698).Curated
Sequence conflicti468 – 4681R → K in CAA47317 (PubMed:7555698).Curated
Sequence conflicti492 – 4921A → D in AAB09548 (Ref. 3) Curated
Sequence conflicti503 – 5031R → Q in CAA47317 (PubMed:7555698).Curated
Sequence conflicti506 – 5061A → G in AAB09548 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY538775 mRNA. Translation: AAS46251.1.
X66841 mRNA. Translation: CAA47317.1.
U64962 mRNA. Translation: AAB09548.1.
PIRiS26595.
RefSeqiNP_001001443.1. NM_001001443.1.
UniGeneiBt.32558.

Genome annotation databases

GeneIDi407238.
KEGGibta:407238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY538775 mRNA. Translation: AAS46251.1.
X66841 mRNA. Translation: CAA47317.1.
U64962 mRNA. Translation: AAB09548.1.
PIRiS26595.
RefSeqiNP_001001443.1. NM_001001443.1.
UniGeneiBt.32558.

3D structure databases

ProteinModelPortaliP49884.
SMRiP49884. Positions 181-254, 302-551.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi160595. 1 interaction.
STRINGi9913.ENSBTAP00000009422.

Chemistry

BindingDBiP49884.
ChEMBLiCHEMBL2095170.

PTM databases

UniCarbKBiP49884.

Proteomic databases

PaxDbiP49884.
PRIDEiP49884.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi407238.
KEGGibta:407238.

Organism-specific databases

CTDi2099.

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
HOGENOMiHOG000233468.
HOVERGENiHBG108344.
InParanoidiP49884.
KOiK08550.

Miscellaneous databases

NextBioi20818487.

Family and domain databases

Gene3Di1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProiIPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR024178. Oest_rcpt/oest-rel_rcp.
IPR001292. Oestr_rcpt.
IPR024736. Oestrogen-typ_rcpt_final_C_dom.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF12743. ESR1_C. 1 hit.
PF00104. Hormone_recep. 1 hit.
PF02159. Oest_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PIRSFiPIRSF500101. ER-a. 1 hit.
PIRSF002527. ER-like_NR. 1 hit.
PRINTSiPR00543. OESTROGENR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Nishimura N., Tetsuka M.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Uterus.
  2. "Expression of estrogen and androgen receptor in the bovine gastrointestinal tract."
    Sauerwein H., Pfaffl M., Hagen-Mann K., Malucelli A., Meyer H.H.
    Dtsch. Tierarztl. Wochenschr. 102:164-168(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 371-503.
    Strain: Simmental.
    Tissue: Uterus.
  3. "Bovine estrogen receptor hormone-binding domain, exons V to VII."
    Malayer J.R.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 386-544.
  4. "A subpopulation of estrogen receptors are modified by O-linked N-acetylglucosamine."
    Jiang M.S., Hart G.W.
    J. Biol. Chem. 272:2421-2428(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION.

Entry informationi

Entry nameiESR1_BOVIN
AccessioniPrimary (citable) accession number: P49884
Secondary accession number(s): Q6QIS5, Q95131
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 9, 2004
Last modified: May 11, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.