Reviewed, UniProtKB/Swiss-Prot P49872 (F261_BOVIN)

Names and origin

Protein names

Recommended name:
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Alternative name(s):
    6PF-2-K/Fru-2,6-P2ASE liver isozyme
Including the following 2 domains:
    1- Recommended name:
            6-phosphofructo-2-kinase
              EC=2.7.1.105
    2- Recommended name:
            Fructose-2,6-bisphosphatase
              EC=3.1.3.46

Gene names

Name:

PFKFB1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Synthesis and degradation of fructose 2,6-bisphosphate.
Catalytic activity	Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.
Enzyme regulation	Phosphorylation results in inhibition of the kinase activity By similarity.
Subunit structure	Homodimer By similarity.
Tissue specificity	Liver By similarity.
Sequence similarities	In the C-terminal section; belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
PTM	Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	fructose 2,6-bisphosphate metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	6-phosphofructo-2-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose-2,6-bisphosphate 2-phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1		PRO_0000179959
Regions
	Nucleotide binding	49 – 56	8	ATP Potential
	Region	1 – 250	250	6-phosphofructo-2-kinase
	Region	251 – 471	221	Fructose-2,6-bisphosphatase
Sites
	Active site	131	1	Potential
	Active site	161	1	Potential
	Active site	259	1	Tele-phosphohistidine intermediate
	Active site	328	1	Potential
	Active site	393	1	Proton donor By similarity
	Binding site	105	1	Fructose-6-phosphate By similarity
	Binding site	196	1	Fructose-6-phosphate By similarity
Amino acid modifications
	Modified residue	33	1	Phosphoserine; by PKA By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49872-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 79C330B873B2D9EB	FASTA	471	54,657
	10 20 30 40 50 60 MSQEMGELTQ TRLQKIWIPH NNGNSRLQRR RGSSIPQFTN SPTMVIMVGL PARGKTYIST 70 80 90 100 110 120 KLTRYLNWIG TPTKVFNLGQ YRREAVSYKN YEFFLPDNME ALLIRKQCAL AALKDVHSYL 130 140 150 160 170 180 SHEEGRVAVF DATNTTRERR SLILQFAKEH GYKVFFIESI CNDPDVIAEN IRQVKLGSPD 190 200 210 220 230 240 YIDCDREKVL EDFLKRIECY EVNYQPLDDE LDSHLSYIKI FDVGTRYMVN RVQDHIQSRT 250 260 270 280 290 300 VYYLMNIHVT PRSIYLCRHG ESELNLRGRI GGDSGLSARG KQYAYALANF IQSQGISSLK 310 320 330 340 350 360 VGTSHMKRTI QTAEALGLPY EQWKALNEID AGVCEEMTYE EIQEHYPEEF ALRDQDKYRY 370 380 390 400 410 420 RYPKGESYED LVQRLEPVIM ELERQENVLV ICHQAVMRCL LAYFLDKSSD ELPYLKCPLH 430 440 450 460 470 TVLKLTPVAY GCKVESIYLN VEAVNTHREK PENVDITREP EEALDTVPAH Y

References

[1]

"Isolation of bovine liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase cDNA: bovine liver and heart forms of the enzyme are separate gene products." Lange A.J., El-Maghrabi M.R., Pilkis S.J. Arch. Biochem. Biophys. 290:258-263(1991) [PubMed: 1654864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M64323 mRNA. Translation: AAA30696.1. S55569 mRNA. Translation: AAB19845.1.
IPI	IPI00708146.
PIR	A44872.
RefSeq	NP_776997.3.
UniGene	Bt.562
3D structure databases
HSSP	HSSP built from PDB template 1C80 based on UniProtKB P07953.
SMR	P49872. Positions 42-470.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000000172. Bos taurus. [Contig view]
GeneID	282304.
KEGG	bta:282304.
Phylogenomic databases
HOVERGEN	P49872.
Enzyme and pathway databases
BRENDA	2.7.1.105. 251. 3.1.3.46. 251.
Family and domain databases
InterPro	IPR003094. 6Pfruct_kin. IPR013079. 6Phosfructo_kin. IPR016260. Bifunct_6PFK/fruc_bisP_Ptase. IPR001345. PG/BPGM_mutase. IPR013078. PG_mutase. [Graphical view]
PANTHER	PTHR10606. 6Pfruct_kin. 1 hit.
Pfam	PF01591. 6PF2K. 1 hit. PF00300. PGAM. 1 hit. [Graphical view]
PIRSF	PIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTS	PR00991. 6PFRUCTKNASE.
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F261_BOVIN

Accession

Primary (citable) accession number: P49872

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)