Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P49872 (F261_BOVIN)

Last modified June 16, 2009. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Alternative name(s):
    6PF-2-K/Fru-2,6-P2ASE liver isozyme
Including the following 2 domains:
    1- Recommended name:
            6-phosphofructo-2-kinase
              EC=2.7.1.105
    2- Recommended name:
            Fructose-2,6-bisphosphatase
              EC=3.1.3.46
Gene names
Name: PFKFB1
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Synthesis and degradation of fructose 2,6-bisphosphate.

Catalytic activity

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.

ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Enzyme regulation

Phosphorylation results in inhibition of the kinase activity By similarity.

Subunit structure

Homodimer By similarity.

Tissue specificity

Liver By similarity.

Sequence similarities

In the C-terminal section; belongs to the phosphoglycerate mutase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4714716-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
PRO_0000179959

Regions

Nucleotide binding49 – 568ATP Potential
Region1 – 2502506-phosphofructo-2-kinase
Region251 – 471221Fructose-2,6-bisphosphatase

Sites

Active site1311 Potential
Active site1611 Potential
Active site2591Tele-phosphohistidine intermediate
Active site3281 Potential
Active site3931Proton donor By similarity
Binding site1051Fructose-6-phosphate By similarity
Binding site1961Fructose-6-phosphate By similarity

Amino acid modifications

Modified residue331Phosphoserine; by PKA By similarity

Sequences

Sequence LengthMass (Da)Tools
P49872-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 79C330B873B2D9EB

FASTA47154,657
        10         20         30         40         50         60 
MSQEMGELTQ TRLQKIWIPH NNGNSRLQRR RGSSIPQFTN SPTMVIMVGL PARGKTYIST 

        70         80         90        100        110        120 
KLTRYLNWIG TPTKVFNLGQ YRREAVSYKN YEFFLPDNME ALLIRKQCAL AALKDVHSYL 

       130        140        150        160        170        180 
SHEEGRVAVF DATNTTRERR SLILQFAKEH GYKVFFIESI CNDPDVIAEN IRQVKLGSPD 

       190        200        210        220        230        240 
YIDCDREKVL EDFLKRIECY EVNYQPLDDE LDSHLSYIKI FDVGTRYMVN RVQDHIQSRT 

       250        260        270        280        290        300 
VYYLMNIHVT PRSIYLCRHG ESELNLRGRI GGDSGLSARG KQYAYALANF IQSQGISSLK 

       310        320        330        340        350        360 
VGTSHMKRTI QTAEALGLPY EQWKALNEID AGVCEEMTYE EIQEHYPEEF ALRDQDKYRY 

       370        380        390        400        410        420 
RYPKGESYED LVQRLEPVIM ELERQENVLV ICHQAVMRCL LAYFLDKSSD ELPYLKCPLH 

       430        440        450        460        470 
TVLKLTPVAY GCKVESIYLN VEAVNTHREK PENVDITREP EEALDTVPAH Y 

« Hide

References

[1]"Isolation of bovine liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase cDNA: bovine liver and heart forms of the enzyme are separate gene products."
Lange A.J., El-Maghrabi M.R., Pilkis S.J.
Arch. Biochem. Biophys. 290:258-263(1991) [PubMed: 1654864] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.

Cross-references

Sequence databases

M64323 mRNA. Translation: AAA30696.1.
S55569 mRNA. Translation: AAB19845.1.
IPIIPI00708146.
PIRA44872.
RefSeqNP_776997.3.
UniGeneBt.562

3D structure databases

HSSPHSSP built from PDB template 1C80 based on UniProtKB P07953.
SMRP49872. Positions 42-470.
ModBaseSearch...

Genome annotation databases

EnsemblENSBTAG00000000172. Bos taurus. [Contig view]
GeneID282304.
KEGGbta:282304.

Phylogenomic databases

HOVERGENP49872.

Enzyme and pathway databases

BRENDA2.7.1.105. 251.
3.1.3.46. 251.

Family and domain databases

InterProIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR016260. Bifunct_6PFK/fruc_bisP_Ptase.
IPR001345. PG/BPGM_mutase.
IPR013078. PG_mutase.
[Graphical view]
PANTHERPTHR10606. 6Pfruct_kin. 1 hit.
PfamPF01591. 6PF2K. 1 hit.
PF00300. PGAM. 1 hit.
[Graphical view]
PIRSFPIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTSPR00991. 6PFRUCTKNASE.
PROSITEPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF261_BOVIN
AccessionPrimary (citable) accession number: P49872
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents