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Protein

Probable nuclear hormone receptor HR38

Gene

Hr38

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to NGFI-B response elements. Plays an important role in late stages of epidermal metamorphosis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi741 – 81676Nuclear receptorPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri744 – 76421NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri780 – 80425NR C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cuticle development Source: FlyBase
  • epidermis development Source: FlyBase
  • intracellular receptor signaling pathway Source: GOC
  • phagocytosis Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • regulation of adult chitin-containing cuticle pigmentation Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-198693. AKT phosphorylates targets in the nucleus.
R-DME-383280. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable nuclear hormone receptor HR38
Short name:
dHR38
Alternative name(s):
Nuclear receptor subfamily 4 group A member 4
Gene namesi
Name:Hr38
Synonyms:NR4A4
ORF Names:CG1864
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0014859. Hr38.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10731073Probable nuclear hormone receptor HR38PRO_0000053725Add
BLAST

Proteomic databases

PaxDbiP49869.
PRIDEiP49869.

Expressioni

Tissue specificityi

Ubiquitously expressed in preblastoderm embryos, specifically in central nervous system and intestinal tract. Highly expressed in third instar larval imaginal disks and brain complexes, but not in ovaries.1 Publication

Developmental stagei

Low levels in 0-8 hours embryos and adults. Higher in late embryogenesis and during larval and pupal stages. Short isoform is enriched in pupae and adults, long isoform in larvae.

Gene expression databases

BgeeiP49869.
ExpressionAtlasiP49869. differential.
GenevisibleiP49869. DM.

Interactioni

Subunit structurei

Forms a heterodimer with USP.

Protein-protein interaction databases

DIPiDIP-929N.
IntActiP49869. 2 interactions.
STRINGi7227.FBpp0080927.

Structurei

Secondary structure

1
1073
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi844 – 85411Combined sources
Helixi859 – 8613Combined sources
Turni865 – 8673Combined sources
Helixi874 – 89623Combined sources
Helixi902 – 9043Combined sources
Helixi907 – 92923Combined sources
Beta strandi935 – 9384Combined sources
Beta strandi942 – 9465Combined sources
Helixi947 – 9548Combined sources
Helixi957 – 97115Combined sources
Helixi975 – 98612Combined sources
Helixi996 – 101621Combined sources
Helixi1018 – 10214Combined sources
Helixi1026 – 10316Combined sources
Helixi1033 – 105422Combined sources
Helixi1061 – 10655Combined sources
Turni1066 – 10683Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PDUX-ray2.30A/B842-1073[»]
DisProtiDP00594.
ProteinModelPortaliP49869.
SMRiP49869. Positions 744-830, 842-1070.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49869.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi188 – 1925Poly-Ala
Compositional biasi206 – 21813Poly-AlaAdd
BLAST
Compositional biasi221 – 2288Poly-Ala
Compositional biasi268 – 2725Poly-Thr
Compositional biasi294 – 31219Poly-GlnAdd
BLAST
Compositional biasi441 – 46222Poly-GlnAdd
BLAST
Compositional biasi505 – 5084Poly-Ser
Compositional biasi619 – 6268Poly-Gln
Compositional biasi661 – 6655Poly-Ala

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri744 – 76421NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri780 – 80425NR C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4217. Eukaryota.
ENOG410YWNC. LUCA.
HOGENOMiHOG000046504.
InParanoidiP49869.
KOiK08558.
OrthoDBiEOG75XGKJ.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P49869-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMRDRLASLI VVKQEGGSNT SISHHQATAI KCEASLYTES SLFQEINNNS
60 70 80 90 100
CYRQNLNAPT HQQSHTSHLQ HAQQHQTHQQ HPLLPPPLPT LPLIYPCRNL
110 120 130 140 150
FPDGCDINHL ACCSSSNSNS NCNSDSNSTS SSPGNSHFFA NGNTCAAALT
160 170 180 190 200
PAPPATEPRK IKPLGAGKLK VGKTDSNSDS NSNCDSRAAA AASTSATSAT
210 220 230 240 250
SATTLAATAA ATAAAAEAGG AASAAAAAKI SQVRLTNQAT TSMLLLQPNS
260 270 280 290 300
SFSSLSPFDN FSTQTASTTT TTSASAAGHH QHHNHLLHQQ HHNQQQQQQQ
310 320 330 340 350
QQQQQQQQQQ QQEHLQQQHQ QQLVSPQQHL LKSETLLSHE EDQLISNLTD
360 370 380 390 400
SSVVSHSELF SDLFFPSDSN NSLLSPTTSG YPDNPAEDLT SSIENLTKLT
410 420 430 440 450
CLRDKRLSSI PEQQLSSEQE QQLCLLSLRS SSDPAIALHA QQQQQQQQQQ
460 470 480 490 500
QQQQQQHQQQ QQHLQLQLIS PIGGPLSCGS SLPSFQETYS LKYNSSSGSS
510 520 530 540 550
PQQASSSSTA APTPTDQVLT LKMDEDCFPP LSGGWSASPP APSQLQQLHT
560 570 580 590 600
LQSQAQMSHP NSSNNSSNNA GNSHNNSGGY NYHGHFNAIN ASANLSPSSS
610 620 630 640 650
ASSLYEYNGV SAADNFYGQQ QQQQQQSYQQ HNYNSHNGER YSLPTFPTIS
660 670 680 690 700
ELAAATAAVE AAAAATVGGP PPVRRASLPV QRTVSPAGST AQSPKLAKIT
710 720 730 740 750
LNQRHSHAHA HALQLNSAPN SAASSPASAD LQAGRLLQAP SQLCAVCGDT
760 770 780 790 800
AACQHYGVRT CEGCKGFFKR TVQKGSKYVC LADKNCPVDK RRRNRCQFCR
810 820 830 840 850
FQKCLVVGMV KEVVRTDSLK GRRGRLPSKP KSPQESPPSP PISLITALVR
860 870 880 890 900
SHVDTTPDPS CLDYSHYEEQ SMSEADKVQQ FYQLLTSSVD VIKQFAEKIP
910 920 930 940 950
GYFDLLPEDQ ELLFQSASLE LFVLRLAYRA RIDDTKLIFC NGTVLHRTQC
960 970 980 990 1000
LRSFGEWLND IMEFSRSLHN LEIDISAFAC LCALTLITER HGLREPKKVE
1010 1020 1030 1040 1050
QLQMKIIGSL RDHVTYNAEA QKKQHYFSRL LGKLPELRSL SVQGLQRIFY
1060 1070
LKLEDLVPAP ALIENMFVTT LPF
Length:1,073
Mass (Da):116,992
Last modified:December 1, 2000 - v3
Checksum:i126A30DAFA1C096A
GO
Isoform Short (identifier: P49869-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-522: Missing.

Show »
Length:551
Mass (Da):60,797
Checksum:i739ACB447F8F45DA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti667 – 6671V → VSSPSV in AAF53914 (PubMed:10731132).Curated
Sequence conflicti685 – 6851S → L (PubMed:7644522).Curated
Sequence conflicti685 – 6851S → L (PubMed:9649534).Curated
Sequence conflicti689 – 6924STAQ → LHGER in CAA75690 (PubMed:9704500).Curated
Sequence conflicti697 – 6971A → D in CAA75690 (PubMed:9704500).Curated
Sequence conflicti702 – 7021N → S in CAA75690 (PubMed:9704500).Curated
Sequence conflicti1041 – 10411S → R in CAA75690 (PubMed:9704500).Curated
Sequence conflicti1064 – 10641E → D in CAA75690 (PubMed:9704500).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 522522Missing in isoform Short. 1 PublicationVSP_003714Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89246 mRNA. Translation: CAA61534.1.
Y15606 Genomic DNA. Translation: CAA75690.1.
AJ002073 mRNA. Translation: CAA05172.1.
AE014134 Genomic DNA. Translation: AAF53914.1.
U36762 mRNA. Translation: AAC46926.1.
RefSeqiNP_477119.1. NM_057771.4.
UniGeneiDm.2701.

Genome annotation databases

GeneIDi35332.
KEGGidme:Dmel_CG1864.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89246 mRNA. Translation: CAA61534.1.
Y15606 Genomic DNA. Translation: CAA75690.1.
AJ002073 mRNA. Translation: CAA05172.1.
AE014134 Genomic DNA. Translation: AAF53914.1.
U36762 mRNA. Translation: AAC46926.1.
RefSeqiNP_477119.1. NM_057771.4.
UniGeneiDm.2701.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PDUX-ray2.30A/B842-1073[»]
DisProtiDP00594.
ProteinModelPortaliP49869.
SMRiP49869. Positions 744-830, 842-1070.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-929N.
IntActiP49869. 2 interactions.
STRINGi7227.FBpp0080927.

Proteomic databases

PaxDbiP49869.
PRIDEiP49869.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi35332.
KEGGidme:Dmel_CG1864.

Organism-specific databases

CTDi35332.
FlyBaseiFBgn0014859. Hr38.

Phylogenomic databases

eggNOGiKOG4217. Eukaryota.
ENOG410YWNC. LUCA.
HOGENOMiHOG000046504.
InParanoidiP49869.
KOiK08558.
OrthoDBiEOG75XGKJ.

Enzyme and pathway databases

ReactomeiR-DME-198693. AKT phosphorylates targets in the nucleus.
R-DME-383280. Nuclear Receptor transcription pathway.

Miscellaneous databases

ChiTaRSiHr38. fly.
EvolutionaryTraceiP49869.
GenomeRNAii35332.
PROiP49869.

Gene expression databases

BgeeiP49869.
ExpressionAtlasiP49869. differential.
GenevisibleiP49869. DM.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Drosophila hormone receptor 38: a second partner for Drosophila USP suggests an unexpected role for nuclear receptors of the nerve growth factor-induced protein B type."
    Sutherland J.D., Kozlova T., Tzertzinis G., Kafatos F.C.
    Proc. Natl. Acad. Sci. U.S.A. 92:7966-7970(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    Tissue: Larva.
  2. "Genomic organization of DHR38 gene in Drosophila: presence of Alu-like repeat in a translated exon and expression during embryonic development."
    Komonyi O., Mink M., Csiha J., Maroy P.
    Arch. Insect Biochem. Physiol. 38:185-192(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT), TISSUE SPECIFICITY.
  3. "Drosophila hormone receptor 38 functions in metamorphosis: a role in adult cuticle formation."
    Kozlova T., Pokholkova G.V., Tzertzinis G., Sutherland J.D., Zhimulev I.F., Kafatos F.C.
    Genetics 149:1465-1475(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), CHARACTERIZATION.
    Tissue: Larva.
  4. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM LONG).
    Strain: Berkeley.
  5. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  6. "Isolation, regulation, and DNA-binding properties of three Drosophila nuclear hormone receptor superfamily members."
    Fisk G.J., Thummel C.S.
    Proc. Natl. Acad. Sci. U.S.A. 92:10604-10608(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 528-1073.
    Strain: Canton-S.

Entry informationi

Entry nameiHR38_DROME
AccessioniPrimary (citable) accession number: P49869
Secondary accession number(s): O18383, Q9VIK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 1, 2000
Last modified: June 8, 2016
This is version 150 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.