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Protein

Probable nuclear hormone receptor HR38

Gene

Hr38

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to NGFI-B response elements. Plays an important role in late stages of epidermal metamorphosis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi741 – 816Nuclear receptorPROSITE-ProRule annotationAdd BLAST76
Zinc fingeri744 – 764NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri780 – 804NR C4-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • cuticle development Source: FlyBase
  • phagocytosis Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • regulation of adult chitin-containing cuticle pigmentation Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-198693. AKT phosphorylates targets in the nucleus.
R-DME-383280. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable nuclear hormone receptor HR38
Short name:
dHR38
Alternative name(s):
Nuclear receptor subfamily 4 group A member 4
Gene namesi
Name:Hr38
Synonyms:NR4A4
ORF Names:CG1864
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0014859. Hr38.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000537251 – 1073Probable nuclear hormone receptor HR38Add BLAST1073

Proteomic databases

PaxDbiP49869.
PRIDEiP49869.

Expressioni

Tissue specificityi

Ubiquitously expressed in preblastoderm embryos, specifically in central nervous system and intestinal tract. Highly expressed in third instar larval imaginal disks and brain complexes, but not in ovaries.1 Publication

Developmental stagei

Low levels in 0-8 hours embryos and adults. Higher in late embryogenesis and during larval and pupal stages. Short isoform is enriched in pupae and adults, long isoform in larvae.

Gene expression databases

BgeeiFBgn0014859.
ExpressionAtlasiP49869. baseline.
GenevisibleiP49869. DM.

Interactioni

Subunit structurei

Forms a heterodimer with USP.

Protein-protein interaction databases

DIPiDIP-929N.
IntActiP49869. 2 interactors.
STRINGi7227.FBpp0080927.

Structurei

Secondary structure

11073
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi844 – 854Combined sources11
Helixi859 – 861Combined sources3
Turni865 – 867Combined sources3
Helixi874 – 896Combined sources23
Helixi902 – 904Combined sources3
Helixi907 – 929Combined sources23
Beta strandi935 – 938Combined sources4
Beta strandi942 – 946Combined sources5
Helixi947 – 954Combined sources8
Helixi957 – 971Combined sources15
Helixi975 – 986Combined sources12
Helixi996 – 1016Combined sources21
Helixi1018 – 1021Combined sources4
Helixi1026 – 1031Combined sources6
Helixi1033 – 1054Combined sources22
Helixi1061 – 1065Combined sources5
Turni1066 – 1068Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PDUX-ray2.30A/B842-1073[»]
DisProtiDP00594.
ProteinModelPortaliP49869.
SMRiP49869.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49869.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi188 – 192Poly-Ala5
Compositional biasi206 – 218Poly-AlaAdd BLAST13
Compositional biasi221 – 228Poly-Ala8
Compositional biasi268 – 272Poly-Thr5
Compositional biasi294 – 312Poly-GlnAdd BLAST19
Compositional biasi441 – 462Poly-GlnAdd BLAST22
Compositional biasi505 – 508Poly-Ser4
Compositional biasi619 – 626Poly-Gln8
Compositional biasi661 – 665Poly-Ala5

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri744 – 764NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri780 – 804NR C4-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4217. Eukaryota.
ENOG410YWNC. LUCA.
HOGENOMiHOG000046504.
InParanoidiP49869.
KOiK08558.
OrthoDBiEOG091G0GUM.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P49869-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMRDRLASLI VVKQEGGSNT SISHHQATAI KCEASLYTES SLFQEINNNS
60 70 80 90 100
CYRQNLNAPT HQQSHTSHLQ HAQQHQTHQQ HPLLPPPLPT LPLIYPCRNL
110 120 130 140 150
FPDGCDINHL ACCSSSNSNS NCNSDSNSTS SSPGNSHFFA NGNTCAAALT
160 170 180 190 200
PAPPATEPRK IKPLGAGKLK VGKTDSNSDS NSNCDSRAAA AASTSATSAT
210 220 230 240 250
SATTLAATAA ATAAAAEAGG AASAAAAAKI SQVRLTNQAT TSMLLLQPNS
260 270 280 290 300
SFSSLSPFDN FSTQTASTTT TTSASAAGHH QHHNHLLHQQ HHNQQQQQQQ
310 320 330 340 350
QQQQQQQQQQ QQEHLQQQHQ QQLVSPQQHL LKSETLLSHE EDQLISNLTD
360 370 380 390 400
SSVVSHSELF SDLFFPSDSN NSLLSPTTSG YPDNPAEDLT SSIENLTKLT
410 420 430 440 450
CLRDKRLSSI PEQQLSSEQE QQLCLLSLRS SSDPAIALHA QQQQQQQQQQ
460 470 480 490 500
QQQQQQHQQQ QQHLQLQLIS PIGGPLSCGS SLPSFQETYS LKYNSSSGSS
510 520 530 540 550
PQQASSSSTA APTPTDQVLT LKMDEDCFPP LSGGWSASPP APSQLQQLHT
560 570 580 590 600
LQSQAQMSHP NSSNNSSNNA GNSHNNSGGY NYHGHFNAIN ASANLSPSSS
610 620 630 640 650
ASSLYEYNGV SAADNFYGQQ QQQQQQSYQQ HNYNSHNGER YSLPTFPTIS
660 670 680 690 700
ELAAATAAVE AAAAATVGGP PPVRRASLPV QRTVSPAGST AQSPKLAKIT
710 720 730 740 750
LNQRHSHAHA HALQLNSAPN SAASSPASAD LQAGRLLQAP SQLCAVCGDT
760 770 780 790 800
AACQHYGVRT CEGCKGFFKR TVQKGSKYVC LADKNCPVDK RRRNRCQFCR
810 820 830 840 850
FQKCLVVGMV KEVVRTDSLK GRRGRLPSKP KSPQESPPSP PISLITALVR
860 870 880 890 900
SHVDTTPDPS CLDYSHYEEQ SMSEADKVQQ FYQLLTSSVD VIKQFAEKIP
910 920 930 940 950
GYFDLLPEDQ ELLFQSASLE LFVLRLAYRA RIDDTKLIFC NGTVLHRTQC
960 970 980 990 1000
LRSFGEWLND IMEFSRSLHN LEIDISAFAC LCALTLITER HGLREPKKVE
1010 1020 1030 1040 1050
QLQMKIIGSL RDHVTYNAEA QKKQHYFSRL LGKLPELRSL SVQGLQRIFY
1060 1070
LKLEDLVPAP ALIENMFVTT LPF
Length:1,073
Mass (Da):116,992
Last modified:December 1, 2000 - v3
Checksum:i126A30DAFA1C096A
GO
Isoform Short (identifier: P49869-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-522: Missing.

Show »
Length:551
Mass (Da):60,797
Checksum:i739ACB447F8F45DA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti667V → VSSPSV in AAF53914 (PubMed:10731132).Curated1
Sequence conflicti685S → L (PubMed:7644522).Curated1
Sequence conflicti685S → L (PubMed:9649534).Curated1
Sequence conflicti689 – 692STAQ → LHGER in CAA75690 (PubMed:9704500).Curated4
Sequence conflicti697A → D in CAA75690 (PubMed:9704500).Curated1
Sequence conflicti702N → S in CAA75690 (PubMed:9704500).Curated1
Sequence conflicti1041S → R in CAA75690 (PubMed:9704500).Curated1
Sequence conflicti1064E → D in CAA75690 (PubMed:9704500).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0037141 – 522Missing in isoform Short. 1 PublicationAdd BLAST522

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89246 mRNA. Translation: CAA61534.1.
Y15606 Genomic DNA. Translation: CAA75690.1.
AJ002073 mRNA. Translation: CAA05172.1.
AE014134 Genomic DNA. Translation: AAF53914.1.
U36762 mRNA. Translation: AAC46926.1.
RefSeqiNP_477119.1. NM_057771.4.
UniGeneiDm.2701.

Genome annotation databases

GeneIDi35332.
KEGGidme:Dmel_CG1864.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89246 mRNA. Translation: CAA61534.1.
Y15606 Genomic DNA. Translation: CAA75690.1.
AJ002073 mRNA. Translation: CAA05172.1.
AE014134 Genomic DNA. Translation: AAF53914.1.
U36762 mRNA. Translation: AAC46926.1.
RefSeqiNP_477119.1. NM_057771.4.
UniGeneiDm.2701.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PDUX-ray2.30A/B842-1073[»]
DisProtiDP00594.
ProteinModelPortaliP49869.
SMRiP49869.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-929N.
IntActiP49869. 2 interactors.
STRINGi7227.FBpp0080927.

Proteomic databases

PaxDbiP49869.
PRIDEiP49869.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi35332.
KEGGidme:Dmel_CG1864.

Organism-specific databases

CTDi35332.
FlyBaseiFBgn0014859. Hr38.

Phylogenomic databases

eggNOGiKOG4217. Eukaryota.
ENOG410YWNC. LUCA.
HOGENOMiHOG000046504.
InParanoidiP49869.
KOiK08558.
OrthoDBiEOG091G0GUM.

Enzyme and pathway databases

ReactomeiR-DME-198693. AKT phosphorylates targets in the nucleus.
R-DME-383280. Nuclear Receptor transcription pathway.

Miscellaneous databases

ChiTaRSiHr38. fly.
EvolutionaryTraceiP49869.
GenomeRNAii35332.
PROiP49869.

Gene expression databases

BgeeiFBgn0014859.
ExpressionAtlasiP49869. baseline.
GenevisibleiP49869. DM.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHR38_DROME
AccessioniPrimary (citable) accession number: P49869
Secondary accession number(s): O18383, Q9VIK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.