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P49864 (GRAK_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Granzyme K

EC=3.4.21.-
Alternative name(s):
NK-tryptase-2
Short name=NK-Tryp-2
Gene names
Name:Gzmk
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length258 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Cytoplasmic granule.

Tissue specificity

Speen, lungs and liver non-parenchymal cells.

Sequence similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Propeptide24 – 252Activation peptide
PRO_0000027419
Chain26 – 258233Granzyme K
PRO_0000027420

Regions

Domain26 – 253228Peptidase S1

Sites

Active site661Charge relay system By similarity
Active site1101Charge relay system By similarity
Active site2081Charge relay system By similarity

Amino acid modifications

Disulfide bond51 ↔ 67 By similarity
Disulfide bond143 ↔ 214 By similarity
Disulfide bond175 ↔ 193 By similarity
Disulfide bond204 ↔ 228 By similarity

Sequences

Sequence LengthMass (Da)Tools
P49864 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 988AD71DB08AFBB4

FASTA25828,465
        10         20         30         40         50         60 
MSFSSSALVF LVAGIYMSSE SFHTEIIGGR EVQPHSRPFM ASIQYRGKHI CGGVLIHPQW 

        70         80         90        100        110        120 
VLTAAHCYSR GHSPTVVLGA HSLSKNEPMK QTFEIKEFIP FSGFKSGTND IMLIKLRTAA 

       130        140        150        160        170        180 
ELNKHVQLLH LRSKNYIRDG TKCQVTGWGS TKPDVLTTSD TLQEVTVTII SRKRCNSQSY 

       190        200        210        220        230        240 
YNHKPVITKD MICAGDRRGE KDSCKGDSGG PLICKGVFHA LVSGGYKCGI SNKPGVYTLL 

       250 
TKKYQTWIKS KLAPSSAH 

« Hide

References

[1]"Purification and cloning of a novel serine protease, RNK-Tryp-2, from the granules of a rat NK cell leukemia."
Sayers T.J., Wiltrout T.A., Smyth M.J., Ottaway K.S., Pilaro A.M., Sowder R., Henderson L.E., Sprenger H., Lloyd A.R.
J. Immunol. 152:2289-2297(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-58.
Strain: Fischer 344.
Tissue: Lymphocyte.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L19694 mRNA. Translation: AAA42057.1.
PIRI56220.
RefSeqNP_058815.1. NM_017119.2.
UniGeneRn.11190.

3D structure databases

ProteinModelPortalP49864.
SMRP49864. Positions 25-254.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP49864. 1 interaction.
STRING10116.ENSRNOP00000014319.

Chemistry

ChEMBLCHEMBL4557.

Protein family/group databases

MEROPSS01.146.

Proteomic databases

PaxDbP49864.
PRIDEP49864.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID29165.
KEGGrno:29165.

Organism-specific databases

CTD3003.
RGD68401. Gzmk.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP49864.
KOK08663.
PhylomeDBP49864.

Gene expression databases

GenevestigatorP49864.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio608211.
PROP49864.

Entry information

Entry nameGRAK_RAT
AccessionPrimary (citable) accession number: P49864
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries