Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Granzyme K

Gene

Gzmk

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Charge relay systemBy similarity
Active sitei110 – 1101Charge relay systemBy similarity
Active sitei208 – 2081Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
  2. serine-type peptidase activity Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.146.

Names & Taxonomyi

Protein namesi
Recommended name:
Granzyme K (EC:3.4.21.-)
Alternative name(s):
NK-tryptase-2
Short name:
NK-Tryp-2
Gene namesi
Name:Gzmk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68401. Gzmk.

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Propeptidei24 – 252Activation peptide1 PublicationPRO_0000027419
Chaini26 – 258233Granzyme KPRO_0000027420Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi51 ↔ 67PROSITE-ProRule annotation
Disulfide bondi143 ↔ 214PROSITE-ProRule annotation
Disulfide bondi175 ↔ 193PROSITE-ProRule annotation
Disulfide bondi204 ↔ 228PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP49864.
PRIDEiP49864.

Expressioni

Tissue specificityi

Speen, lungs and liver non-parenchymal cells.

Gene expression databases

GenevestigatoriP49864.

Interactioni

Protein-protein interaction databases

IntActiP49864. 1 interaction.
STRINGi10116.ENSRNOP00000014319.

Structurei

3D structure databases

ProteinModelPortaliP49864.
SMRiP49864. Positions 25-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 253228Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP49864.
KOiK08663.
PhylomeDBiP49864.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49864-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFSSSALVF LVAGIYMSSE SFHTEIIGGR EVQPHSRPFM ASIQYRGKHI
60 70 80 90 100
CGGVLIHPQW VLTAAHCYSR GHSPTVVLGA HSLSKNEPMK QTFEIKEFIP
110 120 130 140 150
FSGFKSGTND IMLIKLRTAA ELNKHVQLLH LRSKNYIRDG TKCQVTGWGS
160 170 180 190 200
TKPDVLTTSD TLQEVTVTII SRKRCNSQSY YNHKPVITKD MICAGDRRGE
210 220 230 240 250
KDSCKGDSGG PLICKGVFHA LVSGGYKCGI SNKPGVYTLL TKKYQTWIKS

KLAPSSAH
Length:258
Mass (Da):28,465
Last modified:October 1, 1996 - v1
Checksum:i988AD71DB08AFBB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19694 mRNA. Translation: AAA42057.1.
PIRiI56220.
RefSeqiNP_058815.1. NM_017119.2.
UniGeneiRn.11190.

Genome annotation databases

GeneIDi29165.
KEGGirno:29165.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19694 mRNA. Translation: AAA42057.1.
PIRiI56220.
RefSeqiNP_058815.1. NM_017119.2.
UniGeneiRn.11190.

3D structure databases

ProteinModelPortaliP49864.
SMRiP49864. Positions 25-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP49864. 1 interaction.
STRINGi10116.ENSRNOP00000014319.

Chemistry

ChEMBLiCHEMBL4557.

Protein family/group databases

MEROPSiS01.146.

Proteomic databases

PaxDbiP49864.
PRIDEiP49864.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29165.
KEGGirno:29165.

Organism-specific databases

CTDi3003.
RGDi68401. Gzmk.

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP49864.
KOiK08663.
PhylomeDBiP49864.

Miscellaneous databases

NextBioi608211.
PROiP49864.

Gene expression databases

GenevestigatoriP49864.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Purification and cloning of a novel serine protease, RNK-Tryp-2, from the granules of a rat NK cell leukemia."
    Sayers T.J., Wiltrout T.A., Smyth M.J., Ottaway K.S., Pilaro A.M., Sowder R., Henderson L.E., Sprenger H., Lloyd A.R.
    J. Immunol. 152:2289-2297(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-58.
    Strain: Fischer 344.
    Tissue: Lymphocyte.

Entry informationi

Entry nameiGRAK_RAT
AccessioniPrimary (citable) accession number: P49864
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.