P49864 (GRAK_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Granzyme K
EC=3.4.21.-

Alternative name(s):
NK-tryptase-2
Short name=NK-Tryp-2

Gene names

Name:

Gzmk

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cytoplasmic granule.
Tissue specificity	Speen, lungs and liver non-parenchymal cells.
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type peptidase activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Propeptide	24 – 25	2	Activation peptide		PRO_0000027419
	Chain	26 – 258	233	Granzyme K		PRO_0000027420
Regions
	Domain	26 – 253	228	Peptidase S1
Sites
	Active site	66	1	Charge relay system By similarity
	Active site	110	1	Charge relay system By similarity
	Active site	208	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	51 ↔ 67		By similarity
	Disulfide bond	143 ↔ 214		By similarity
	Disulfide bond	175 ↔ 193		By similarity
	Disulfide bond	204 ↔ 228		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49864 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 988AD71DB08AFBB4	FASTA	258	28,465
	10 20 30 40 50 60 MSFSSSALVF LVAGIYMSSE SFHTEIIGGR EVQPHSRPFM ASIQYRGKHI CGGVLIHPQW 70 80 90 100 110 120 VLTAAHCYSR GHSPTVVLGA HSLSKNEPMK QTFEIKEFIP FSGFKSGTND IMLIKLRTAA 130 140 150 160 170 180 ELNKHVQLLH LRSKNYIRDG TKCQVTGWGS TKPDVLTTSD TLQEVTVTII SRKRCNSQSY 190 200 210 220 230 240 YNHKPVITKD MICAGDRRGE KDSCKGDSGG PLICKGVFHA LVSGGYKCGI SNKPGVYTLL 250 TKKYQTWIKS KLAPSSAH

References

[1]

"Purification and cloning of a novel serine protease, RNK-Tryp-2, from the granules of a rat NK cell leukemia." Sayers T.J., Wiltrout T.A., Smyth M.J., Ottaway K.S., Pilaro A.M., Sowder R., Henderson L.E., Sprenger H., Lloyd A.R. J. Immunol. 152:2289-2297(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-58. Strain: Fischer 344. Tissue: Lymphocyte.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19694 mRNA. Translation: AAA42057.1.
IPI	IPI00198281.
PIR	I56220.
RefSeq	NP_058815.1. NM_017119.2.
UniGene	Rn.11190.
3D structure databases
ProteinModelPortal	P49864.
SMR	P49864. Positions 25-254.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49864. 1 interaction.
STRING	10116.ENSRNOP00000014319.
Protein family/group databases
MEROPS	S01.146.
Proteomic databases
PaxDb	P49864.
PRIDE	P49864.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	29165.
KEGG	rno:29165.
Organism-specific databases
CTD	3003.
RGD	68401. Gzmk.
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P49864.
KO	K08663.
OrthoDB	EOG4V9TR7.
Gene expression databases
ArrayExpress	P49864.
Genevestigator	P49864.
GermOnline	ENSRNOG00000010661. Rattus norvegicus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL4557.
NextBio	608211.

Entry information

Entry name

GRAK_RAT

Accession

Primary (citable) accession number: P49864

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program