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P49863 (GRAK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Granzyme K

EC=3.4.21.-
Alternative name(s):
Fragmentin-3
Granzyme-3
NK-tryptase-2
Short name=NK-Tryp-2
Gene names
Name:GZMK
Synonyms:TRYP2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Secreted. Cytoplasmic granule.

Tissue specificity

Expressed in lung, spleen, thymus and peripheral blood leukocytes.

Sequence similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Contains 1 peptidase S1 domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 262Activation peptide
PRO_0000027415
Chain27 – 264238Granzyme K
PRO_0000027416

Regions

Domain27 – 259233Peptidase S1

Sites

Active site671Charge relay system
Active site1161Charge relay system
Active site2141Charge relay system

Amino acid modifications

Disulfide bond52 ↔ 68
Disulfide bond149 ↔ 220
Disulfide bond181 ↔ 199
Disulfide bond210 ↔ 234

Experimental info

Sequence conflict341S → Q AA sequence Ref.7
Sequence conflict431S → A AA sequence Ref.7

Secondary structure

....................................... 264
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P49863 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 2A93FDBAF9286CC5

FASTA26428,882
        10         20         30         40         50         60 
MTKFSSFSLF FLIVGAYMTH VCFNMEIIGG KEVSPHSRPF MASIQYGGHH VCGGVLIDPQ 

        70         80         90        100        110        120 
WVLTAAHCQY RFTKGQSPTV VLGAHSLSKN EASKQTLEIK KFIPFSRVTS DPQSNDIMLV 

       130        140        150        160        170        180 
KLQTAAKLNK HVKMLHIRSK TSLRSGTKCK VTGWGATDPD SLRPSDTLRE VTVTVLSRKL 

       190        200        210        220        230        240 
CNSQSYYNGD PFITKDMVCA GDAKGQKDSC KGDSGGPLIC KGVFHAIVSG GHECGVATKP 

       250        260 
GIYTLLTKKY QTWIKSNLVP PHTN 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of cDNA for human granzyme 3."
Przetak M.M., Yoast S., Schmidt B.F.
FEBS Lett. 364:268-271(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Ascites.
[2]"Cloning and expression of a second human natural killer cell granule tryptase, HNK-Tryp-2/granzyme 3."
Sayers T.J., Lloyd A.R., McVicar D.W., O'Connor M.D., Kelly J.M., Carter C.R.D., Wiltrout T.A., Wiltrout R.H., Smyth M.J.
J. Leukoc. Biol. 59:763-768(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lymphocyte.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Thymus.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas.
[6]"Characterization of three serine esterases isolated from human IL-2 activated killer cells."
Hameed A., Lowrey D.M., Lichtenheld M., Podack E.R.
J. Immunol. 141:3142-3147(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 27-42, CHARACTERIZATION.
[7]"Purification of three cytotoxic lymphocyte granule serine proteases that induce apoptosis through distinct substrate and target cell interactions."
Shi L., Kam C.-M., Powers J.C., Aebersold R., Greenberg A.H.
J. Exp. Med. 176:1521-1529(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 27-48.
[8]"The 2.2-A crystal structure of human pro-granzyme K reveals a rigid zymogen with unusual features."
Hink-Schauer C., Estebanez-Perpina E., Wilharm E., Fuentes-Prior P., Klinkert W., Bode W., Jenne D.E.
J. Biol. Chem. 277:50923-50933(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 25-264.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U35237 mRNA. Translation: AAA79063.1.
U26174 mRNA. Translation: AAA74578.1.
AK312074 mRNA. Translation: BAG35010.1.
CH471123 Genomic DNA. Translation: EAW54899.1.
BC035802 mRNA. Translation: AAH35802.1.
CCDSCCDS3964.1.
PIRS65663.
RefSeqNP_002095.1. NM_002104.2.
UniGeneHs.277937.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1MZAX-ray2.23A25-264[»]
1MZDX-ray2.90A25-264[»]
ProteinModelPortalP49863.
SMRP49863. Positions 25-264.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109258. 12 interactions.
IntActP49863. 2 interactions.
STRING9606.ENSP00000231009.

Chemistry

BindingDBP49863.
ChEMBLCHEMBL4930.

Protein family/group databases

MEROPSS01.146.

PTM databases

PhosphoSiteP49863.

Polymorphism databases

DMDM1708035.

Proteomic databases

PaxDbP49863.
PRIDEP49863.

Protocols and materials databases

DNASU3003.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000231009; ENSP00000231009; ENSG00000113088.
GeneID3003.
KEGGhsa:3003.
UCSCuc003jpl.1. human.

Organism-specific databases

CTD3003.
GeneCardsGC05P054320.
HGNCHGNC:4711. GZMK.
MIM600784. gene.
neXtProtNX_P49863.
PharmGKBPA29089.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP49863.
KOK08663.
OMAHECGVAT.
OrthoDBEOG7W9RVH.
PhylomeDBP49863.
TreeFamTF333630.

Gene expression databases

BgeeP49863.
CleanExHS_GZMK.
GenevestigatorP49863.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP49863.
GeneWikiGZMK.
GenomeRNAi3003.
NextBio11908.
PROP49863.
SOURCESearch...

Entry information

Entry nameGRAK_HUMAN
AccessionPrimary (citable) accession number: P49863
Secondary accession number(s): B2R563
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM