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Protein

Granzyme K

Gene

GZMK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Charge relay system
Active sitei116 – 1161Charge relay system
Active sitei214 – 2141Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
  2. serine-type peptidase activity Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.146.

Names & Taxonomyi

Protein namesi
Recommended name:
Granzyme K (EC:3.4.21.-)
Alternative name(s):
Fragmentin-3
Granzyme-3
NK-tryptase-2
Short name:
NK-Tryp-2
Gene namesi
Name:GZMK
Synonyms:TRYP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:4711. GZMK.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29089.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Propeptidei25 – 262Activation peptide2 PublicationsPRO_0000027415
Chaini27 – 264238Granzyme KPRO_0000027416Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi52 ↔ 68
Disulfide bondi149 ↔ 220
Disulfide bondi181 ↔ 199
Disulfide bondi210 ↔ 234

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP49863.
PRIDEiP49863.

PTM databases

PhosphoSiteiP49863.

Expressioni

Tissue specificityi

Expressed in lung, spleen, thymus and peripheral blood leukocytes.

Gene expression databases

BgeeiP49863.
CleanExiHS_GZMK.
GenevestigatoriP49863.

Interactioni

Protein-protein interaction databases

BioGridi109258. 12 interactions.
IntActiP49863. 2 interactions.
STRINGi9606.ENSP00000231009.

Structurei

Secondary structure

1
264
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi41 – 466Combined sources
Beta strandi49 – 5810Combined sources
Beta strandi61 – 644Combined sources
Helixi66 – 683Combined sources
Beta strandi78 – 836Combined sources
Beta strandi85 – 895Combined sources
Beta strandi95 – 10410Combined sources
Beta strandi111 – 1155Combined sources
Beta strandi118 – 1247Combined sources
Beta strandi130 – 1323Combined sources
Beta strandi148 – 1536Combined sources
Beta strandi169 – 1757Combined sources
Helixi178 – 1814Combined sources
Turni184 – 1918Combined sources
Beta strandi197 – 2015Combined sources
Beta strandi217 – 2204Combined sources
Beta strandi223 – 2286Combined sources
Beta strandi241 – 2455Combined sources
Helixi248 – 25710Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MZAX-ray2.23A25-264[»]
1MZDX-ray2.90A25-264[»]
ProteinModelPortaliP49863.
SMRiP49863. Positions 25-264.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49863.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 259233Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP49863.
KOiK08663.
OMAiWIKSNLV.
OrthoDBiEOG7W9RVH.
PhylomeDBiP49863.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49863-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKFSSFSLF FLIVGAYMTH VCFNMEIIGG KEVSPHSRPF MASIQYGGHH
60 70 80 90 100
VCGGVLIDPQ WVLTAAHCQY RFTKGQSPTV VLGAHSLSKN EASKQTLEIK
110 120 130 140 150
KFIPFSRVTS DPQSNDIMLV KLQTAAKLNK HVKMLHIRSK TSLRSGTKCK
160 170 180 190 200
VTGWGATDPD SLRPSDTLRE VTVTVLSRKL CNSQSYYNGD PFITKDMVCA
210 220 230 240 250
GDAKGQKDSC KGDSGGPLIC KGVFHAIVSG GHECGVATKP GIYTLLTKKY
260
QTWIKSNLVP PHTN
Length:264
Mass (Da):28,882
Last modified:October 1, 1996 - v1
Checksum:i2A93FDBAF9286CC5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 341S → Q AA sequence (PubMed:1460416)Curated
Sequence conflicti43 – 431S → A AA sequence (PubMed:1460416)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35237 mRNA. Translation: AAA79063.1.
U26174 mRNA. Translation: AAA74578.1.
AK312074 mRNA. Translation: BAG35010.1.
CH471123 Genomic DNA. Translation: EAW54899.1.
BC035802 mRNA. Translation: AAH35802.1.
CCDSiCCDS3964.1.
PIRiS65663.
RefSeqiNP_002095.1. NM_002104.2.
UniGeneiHs.277937.

Genome annotation databases

EnsembliENST00000231009; ENSP00000231009; ENSG00000113088.
GeneIDi3003.
KEGGihsa:3003.
UCSCiuc003jpl.1. human.

Polymorphism databases

DMDMi1708035.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35237 mRNA. Translation: AAA79063.1.
U26174 mRNA. Translation: AAA74578.1.
AK312074 mRNA. Translation: BAG35010.1.
CH471123 Genomic DNA. Translation: EAW54899.1.
BC035802 mRNA. Translation: AAH35802.1.
CCDSiCCDS3964.1.
PIRiS65663.
RefSeqiNP_002095.1. NM_002104.2.
UniGeneiHs.277937.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MZAX-ray2.23A25-264[»]
1MZDX-ray2.90A25-264[»]
ProteinModelPortaliP49863.
SMRiP49863. Positions 25-264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109258. 12 interactions.
IntActiP49863. 2 interactions.
STRINGi9606.ENSP00000231009.

Chemistry

BindingDBiP49863.
ChEMBLiCHEMBL4930.

Protein family/group databases

MEROPSiS01.146.

PTM databases

PhosphoSiteiP49863.

Polymorphism databases

DMDMi1708035.

Proteomic databases

PaxDbiP49863.
PRIDEiP49863.

Protocols and materials databases

DNASUi3003.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000231009; ENSP00000231009; ENSG00000113088.
GeneIDi3003.
KEGGihsa:3003.
UCSCiuc003jpl.1. human.

Organism-specific databases

CTDi3003.
GeneCardsiGC05P054320.
HGNCiHGNC:4711. GZMK.
MIMi600784. gene.
neXtProtiNX_P49863.
PharmGKBiPA29089.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP49863.
KOiK08663.
OMAiWIKSNLV.
OrthoDBiEOG7W9RVH.
PhylomeDBiP49863.
TreeFamiTF333630.

Miscellaneous databases

EvolutionaryTraceiP49863.
GeneWikiiGZMK.
GenomeRNAii3003.
NextBioi11908.
PROiP49863.
SOURCEiSearch...

Gene expression databases

BgeeiP49863.
CleanExiHS_GZMK.
GenevestigatoriP49863.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ascites.
  2. "Cloning and expression of a second human natural killer cell granule tryptase, HNK-Tryp-2/granzyme 3."
    Sayers T.J., Lloyd A.R., McVicar D.W., O'Connor M.D., Kelly J.M., Carter C.R.D., Wiltrout T.A., Wiltrout R.H., Smyth M.J.
    J. Leukoc. Biol. 59:763-768(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lymphocyte.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas.
  6. "Characterization of three serine esterases isolated from human IL-2 activated killer cells."
    Hameed A., Lowrey D.M., Lichtenheld M., Podack E.R.
    J. Immunol. 141:3142-3147(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-42, CHARACTERIZATION.
  7. "Purification of three cytotoxic lymphocyte granule serine proteases that induce apoptosis through distinct substrate and target cell interactions."
    Shi L., Kam C.-M., Powers J.C., Aebersold R., Greenberg A.H.
    J. Exp. Med. 176:1521-1529(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-48.
  8. "The 2.2-A crystal structure of human pro-granzyme K reveals a rigid zymogen with unusual features."
    Hink-Schauer C., Estebanez-Perpina E., Wilharm E., Fuentes-Prior P., Klinkert W., Bode W., Jenne D.E.
    J. Biol. Chem. 277:50923-50933(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 25-264.

Entry informationi

Entry nameiGRAK_HUMAN
AccessioniPrimary (citable) accession number: P49863
Secondary accession number(s): B2R563
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.