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P49863

- GRAK_HUMAN

UniProt

P49863 - GRAK_HUMAN

Protein

Granzyme K

Gene

GZMK

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei67 – 671Charge relay system
    Active sitei116 – 1161Charge relay system
    Active sitei214 – 2141Charge relay system

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro
    2. serine-type peptidase activity Source: ProtInc

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.146.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Granzyme K (EC:3.4.21.-)
    Alternative name(s):
    Fragmentin-3
    Granzyme-3
    NK-tryptase-2
    Short name:
    NK-Tryp-2
    Gene namesi
    Name:GZMK
    Synonyms:TRYP2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:4711. GZMK.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29089.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Propeptidei25 – 262Activation peptide2 PublicationsPRO_0000027415
    Chaini27 – 264238Granzyme KPRO_0000027416Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi52 ↔ 68
    Disulfide bondi149 ↔ 220
    Disulfide bondi181 ↔ 199
    Disulfide bondi210 ↔ 234

    Keywords - PTMi

    Disulfide bond, Zymogen

    Proteomic databases

    PaxDbiP49863.
    PRIDEiP49863.

    PTM databases

    PhosphoSiteiP49863.

    Expressioni

    Tissue specificityi

    Expressed in lung, spleen, thymus and peripheral blood leukocytes.

    Gene expression databases

    BgeeiP49863.
    CleanExiHS_GZMK.
    GenevestigatoriP49863.

    Interactioni

    Protein-protein interaction databases

    BioGridi109258. 12 interactions.
    IntActiP49863. 2 interactions.
    STRINGi9606.ENSP00000231009.

    Structurei

    Secondary structure

    1
    264
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi41 – 466
    Beta strandi49 – 5810
    Beta strandi61 – 644
    Helixi66 – 683
    Beta strandi78 – 836
    Beta strandi85 – 895
    Beta strandi95 – 10410
    Beta strandi111 – 1155
    Beta strandi118 – 1247
    Beta strandi130 – 1323
    Beta strandi148 – 1536
    Beta strandi169 – 1757
    Helixi178 – 1814
    Turni184 – 1918
    Beta strandi197 – 2015
    Beta strandi217 – 2204
    Beta strandi223 – 2286
    Beta strandi241 – 2455
    Helixi248 – 25710

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MZAX-ray2.23A25-264[»]
    1MZDX-ray2.90A25-264[»]
    ProteinModelPortaliP49863.
    SMRiP49863. Positions 25-264.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP49863.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 259233Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP49863.
    KOiK08663.
    OMAiHECGVAT.
    OrthoDBiEOG7W9RVH.
    PhylomeDBiP49863.
    TreeFamiTF333630.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49863-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKFSSFSLF FLIVGAYMTH VCFNMEIIGG KEVSPHSRPF MASIQYGGHH    50
    VCGGVLIDPQ WVLTAAHCQY RFTKGQSPTV VLGAHSLSKN EASKQTLEIK 100
    KFIPFSRVTS DPQSNDIMLV KLQTAAKLNK HVKMLHIRSK TSLRSGTKCK 150
    VTGWGATDPD SLRPSDTLRE VTVTVLSRKL CNSQSYYNGD PFITKDMVCA 200
    GDAKGQKDSC KGDSGGPLIC KGVFHAIVSG GHECGVATKP GIYTLLTKKY 250
    QTWIKSNLVP PHTN 264
    Length:264
    Mass (Da):28,882
    Last modified:October 1, 1996 - v1
    Checksum:i2A93FDBAF9286CC5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti34 – 341S → Q AA sequence (PubMed:1460416)Curated
    Sequence conflicti43 – 431S → A AA sequence (PubMed:1460416)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35237 mRNA. Translation: AAA79063.1.
    U26174 mRNA. Translation: AAA74578.1.
    AK312074 mRNA. Translation: BAG35010.1.
    CH471123 Genomic DNA. Translation: EAW54899.1.
    BC035802 mRNA. Translation: AAH35802.1.
    CCDSiCCDS3964.1.
    PIRiS65663.
    RefSeqiNP_002095.1. NM_002104.2.
    UniGeneiHs.277937.

    Genome annotation databases

    EnsembliENST00000231009; ENSP00000231009; ENSG00000113088.
    GeneIDi3003.
    KEGGihsa:3003.
    UCSCiuc003jpl.1. human.

    Polymorphism databases

    DMDMi1708035.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35237 mRNA. Translation: AAA79063.1 .
    U26174 mRNA. Translation: AAA74578.1 .
    AK312074 mRNA. Translation: BAG35010.1 .
    CH471123 Genomic DNA. Translation: EAW54899.1 .
    BC035802 mRNA. Translation: AAH35802.1 .
    CCDSi CCDS3964.1.
    PIRi S65663.
    RefSeqi NP_002095.1. NM_002104.2.
    UniGenei Hs.277937.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MZA X-ray 2.23 A 25-264 [» ]
    1MZD X-ray 2.90 A 25-264 [» ]
    ProteinModelPortali P49863.
    SMRi P49863. Positions 25-264.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109258. 12 interactions.
    IntActi P49863. 2 interactions.
    STRINGi 9606.ENSP00000231009.

    Chemistry

    BindingDBi P49863.
    ChEMBLi CHEMBL4930.

    Protein family/group databases

    MEROPSi S01.146.

    PTM databases

    PhosphoSitei P49863.

    Polymorphism databases

    DMDMi 1708035.

    Proteomic databases

    PaxDbi P49863.
    PRIDEi P49863.

    Protocols and materials databases

    DNASUi 3003.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000231009 ; ENSP00000231009 ; ENSG00000113088 .
    GeneIDi 3003.
    KEGGi hsa:3003.
    UCSCi uc003jpl.1. human.

    Organism-specific databases

    CTDi 3003.
    GeneCardsi GC05P054320.
    HGNCi HGNC:4711. GZMK.
    MIMi 600784. gene.
    neXtProti NX_P49863.
    PharmGKBi PA29089.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P49863.
    KOi K08663.
    OMAi HECGVAT.
    OrthoDBi EOG7W9RVH.
    PhylomeDBi P49863.
    TreeFami TF333630.

    Miscellaneous databases

    EvolutionaryTracei P49863.
    GeneWikii GZMK.
    GenomeRNAii 3003.
    NextBioi 11908.
    PROi P49863.
    SOURCEi Search...

    Gene expression databases

    Bgeei P49863.
    CleanExi HS_GZMK.
    Genevestigatori P49863.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ascites.
    2. "Cloning and expression of a second human natural killer cell granule tryptase, HNK-Tryp-2/granzyme 3."
      Sayers T.J., Lloyd A.R., McVicar D.W., O'Connor M.D., Kelly J.M., Carter C.R.D., Wiltrout T.A., Wiltrout R.H., Smyth M.J.
      J. Leukoc. Biol. 59:763-768(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lymphocyte.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pancreas.
    6. "Characterization of three serine esterases isolated from human IL-2 activated killer cells."
      Hameed A., Lowrey D.M., Lichtenheld M., Podack E.R.
      J. Immunol. 141:3142-3147(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 27-42, CHARACTERIZATION.
    7. "Purification of three cytotoxic lymphocyte granule serine proteases that induce apoptosis through distinct substrate and target cell interactions."
      Shi L., Kam C.-M., Powers J.C., Aebersold R., Greenberg A.H.
      J. Exp. Med. 176:1521-1529(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 27-48.
    8. "The 2.2-A crystal structure of human pro-granzyme K reveals a rigid zymogen with unusual features."
      Hink-Schauer C., Estebanez-Perpina E., Wilharm E., Fuentes-Prior P., Klinkert W., Bode W., Jenne D.E.
      J. Biol. Chem. 277:50923-50933(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 25-264.

    Entry informationi

    Entry nameiGRAK_HUMAN
    AccessioniPrimary (citable) accession number: P49863
    Secondary accession number(s): B2R563
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Peptidase families
      Classification of peptidase families and list of entries
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3