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P49863

- GRAK_HUMAN

UniProt

P49863 - GRAK_HUMAN

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Protein

Granzyme K

Gene
GZMK, TRYP2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Charge relay system
Active sitei116 – 1161Charge relay system
Active sitei214 – 2141Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
  2. serine-type peptidase activity Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.146.

Names & Taxonomyi

Protein namesi
Recommended name:
Granzyme K (EC:3.4.21.-)
Alternative name(s):
Fragmentin-3
Granzyme-3
NK-tryptase-2
Short name:
NK-Tryp-2
Gene namesi
Name:GZMK
Synonyms:TRYP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:4711. GZMK.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29089.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed predictionAdd
BLAST
Propeptidei25 – 262Activation peptidePRO_0000027415
Chaini27 – 264238Granzyme KPRO_0000027416Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi52 ↔ 68
Disulfide bondi149 ↔ 220
Disulfide bondi181 ↔ 199
Disulfide bondi210 ↔ 234

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP49863.
PRIDEiP49863.

PTM databases

PhosphoSiteiP49863.

Expressioni

Tissue specificityi

Expressed in lung, spleen, thymus and peripheral blood leukocytes.

Gene expression databases

BgeeiP49863.
CleanExiHS_GZMK.
GenevestigatoriP49863.

Interactioni

Protein-protein interaction databases

BioGridi109258. 12 interactions.
IntActiP49863. 2 interactions.
STRINGi9606.ENSP00000231009.

Structurei

Secondary structure

1
264
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi41 – 466
Beta strandi49 – 5810
Beta strandi61 – 644
Helixi66 – 683
Beta strandi78 – 836
Beta strandi85 – 895
Beta strandi95 – 10410
Beta strandi111 – 1155
Beta strandi118 – 1247
Beta strandi130 – 1323
Beta strandi148 – 1536
Beta strandi169 – 1757
Helixi178 – 1814
Turni184 – 1918
Beta strandi197 – 2015
Beta strandi217 – 2204
Beta strandi223 – 2286
Beta strandi241 – 2455
Helixi248 – 25710

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MZAX-ray2.23A25-264[»]
1MZDX-ray2.90A25-264[»]
ProteinModelPortaliP49863.
SMRiP49863. Positions 25-264.

Miscellaneous databases

EvolutionaryTraceiP49863.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 259233Peptidase S1Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP49863.
KOiK08663.
OMAiHECGVAT.
OrthoDBiEOG7W9RVH.
PhylomeDBiP49863.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49863-1 [UniParc]FASTAAdd to Basket

« Hide

MTKFSSFSLF FLIVGAYMTH VCFNMEIIGG KEVSPHSRPF MASIQYGGHH    50
VCGGVLIDPQ WVLTAAHCQY RFTKGQSPTV VLGAHSLSKN EASKQTLEIK 100
KFIPFSRVTS DPQSNDIMLV KLQTAAKLNK HVKMLHIRSK TSLRSGTKCK 150
VTGWGATDPD SLRPSDTLRE VTVTVLSRKL CNSQSYYNGD PFITKDMVCA 200
GDAKGQKDSC KGDSGGPLIC KGVFHAIVSG GHECGVATKP GIYTLLTKKY 250
QTWIKSNLVP PHTN 264
Length:264
Mass (Da):28,882
Last modified:October 1, 1996 - v1
Checksum:i2A93FDBAF9286CC5
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 341S → Q AA sequence 1 Publication
Sequence conflicti43 – 431S → A AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U35237 mRNA. Translation: AAA79063.1.
U26174 mRNA. Translation: AAA74578.1.
AK312074 mRNA. Translation: BAG35010.1.
CH471123 Genomic DNA. Translation: EAW54899.1.
BC035802 mRNA. Translation: AAH35802.1.
CCDSiCCDS3964.1.
PIRiS65663.
RefSeqiNP_002095.1. NM_002104.2.
UniGeneiHs.277937.

Genome annotation databases

EnsembliENST00000231009; ENSP00000231009; ENSG00000113088.
GeneIDi3003.
KEGGihsa:3003.
UCSCiuc003jpl.1. human.

Polymorphism databases

DMDMi1708035.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U35237 mRNA. Translation: AAA79063.1 .
U26174 mRNA. Translation: AAA74578.1 .
AK312074 mRNA. Translation: BAG35010.1 .
CH471123 Genomic DNA. Translation: EAW54899.1 .
BC035802 mRNA. Translation: AAH35802.1 .
CCDSi CCDS3964.1.
PIRi S65663.
RefSeqi NP_002095.1. NM_002104.2.
UniGenei Hs.277937.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MZA X-ray 2.23 A 25-264 [» ]
1MZD X-ray 2.90 A 25-264 [» ]
ProteinModelPortali P49863.
SMRi P49863. Positions 25-264.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 109258. 12 interactions.
IntActi P49863. 2 interactions.
STRINGi 9606.ENSP00000231009.

Chemistry

BindingDBi P49863.
ChEMBLi CHEMBL4930.

Protein family/group databases

MEROPSi S01.146.

PTM databases

PhosphoSitei P49863.

Polymorphism databases

DMDMi 1708035.

Proteomic databases

PaxDbi P49863.
PRIDEi P49863.

Protocols and materials databases

DNASUi 3003.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000231009 ; ENSP00000231009 ; ENSG00000113088 .
GeneIDi 3003.
KEGGi hsa:3003.
UCSCi uc003jpl.1. human.

Organism-specific databases

CTDi 3003.
GeneCardsi GC05P054320.
HGNCi HGNC:4711. GZMK.
MIMi 600784. gene.
neXtProti NX_P49863.
PharmGKBi PA29089.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi P49863.
KOi K08663.
OMAi HECGVAT.
OrthoDBi EOG7W9RVH.
PhylomeDBi P49863.
TreeFami TF333630.

Miscellaneous databases

EvolutionaryTracei P49863.
GeneWikii GZMK.
GenomeRNAii 3003.
NextBioi 11908.
PROi P49863.
SOURCEi Search...

Gene expression databases

Bgeei P49863.
CleanExi HS_GZMK.
Genevestigatori P49863.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ascites.
  2. "Cloning and expression of a second human natural killer cell granule tryptase, HNK-Tryp-2/granzyme 3."
    Sayers T.J., Lloyd A.R., McVicar D.W., O'Connor M.D., Kelly J.M., Carter C.R.D., Wiltrout T.A., Wiltrout R.H., Smyth M.J.
    J. Leukoc. Biol. 59:763-768(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lymphocyte.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas.
  6. "Characterization of three serine esterases isolated from human IL-2 activated killer cells."
    Hameed A., Lowrey D.M., Lichtenheld M., Podack E.R.
    J. Immunol. 141:3142-3147(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-42, CHARACTERIZATION.
  7. "Purification of three cytotoxic lymphocyte granule serine proteases that induce apoptosis through distinct substrate and target cell interactions."
    Shi L., Kam C.-M., Powers J.C., Aebersold R., Greenberg A.H.
    J. Exp. Med. 176:1521-1529(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-48.
  8. "The 2.2-A crystal structure of human pro-granzyme K reveals a rigid zymogen with unusual features."
    Hink-Schauer C., Estebanez-Perpina E., Wilharm E., Fuentes-Prior P., Klinkert W., Bode W., Jenne D.E.
    J. Biol. Chem. 277:50923-50933(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 25-264.

Entry informationi

Entry nameiGRAK_HUMAN
AccessioniPrimary (citable) accession number: P49863
Secondary accession number(s): B2R563
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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