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Protein

Kallikrein-7

Gene

KLK7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.1 Publication

Catalytic activityi

Cleavage of proteins with aromatic side chains in the P1 position.

Enzyme regulationi

Inhibited by Zn2+ and Cu2+ at low micromolar concentrations. Inhibited by SERPINA12.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei70Charge relay system1 Publication1
Sitei109Major binding site for inhibitory zinc or copper1
Active sitei112Charge relay system1 Publication1
Active sitei205Charge relay system1 Publication1

GO - Molecular functioni

  • metalloendopeptidase activity Source: Reactome
  • peptidase activity Source: UniProtKB
  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: ProtInc

GO - Biological processi

  • epidermis development Source: ProtInc
  • extracellular matrix disassembly Source: Reactome
  • positive regulation of antibacterial peptide production Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciZFISH:HS09870-MONOMER.
BRENDAi3.4.21.117. 2681.
ReactomeiR-HSA-1474228. Degradation of the extracellular matrix.

Protein family/group databases

MEROPSiS01.300.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein-7 (EC:3.4.21.117)
Short name:
hK7
Alternative name(s):
Serine protease 6
Stratum corneum chymotryptic enzyme
Short name:
hSCCE
Gene namesi
Name:KLK7
Synonyms:PRSS6, SCCE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:6368. KLK7.

Subcellular locationi

  • Secreted 1 Publication

  • Note: In ovarian carcinoma, secreted and also observed at the apical membrane and in cytoplasm at the invasive front.

GO - Cellular componenti

  • epidermal lamellar body Source: UniProtKB
  • extracellular region Source: Reactome
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi54H → F: No effect on zinc inhibition. 1 Publication1
Mutagenesisi109H → A: No zinc inhibition. 1 Publication1

Organism-specific databases

DisGeNETi5650.
OpenTargetsiENSG00000169035.
PharmGKBiPA30157.

Chemistry databases

ChEMBLiCHEMBL2443.
GuidetoPHARMACOLOGYi2377.

Polymorphism and mutation databases

BioMutaiKLK7.
DMDMi1710878.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
PropeptideiPRO_000002794223 – 29Activation peptide7
ChainiPRO_000002794330 – 253Kallikrein-7Add BLAST224

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 165
Disulfide bondi55 ↔ 71
Disulfide bondi137 ↔ 239
Disulfide bondi144 ↔ 211
Disulfide bondi176 ↔ 190
Disulfide bondi201 ↔ 226
Glycosylationi246N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP49862.
PeptideAtlasiP49862.
PRIDEiP49862.

PTM databases

iPTMnetiP49862.
PhosphoSitePlusiP49862.

Miscellaneous databases

PMAP-CutDBP49862.

Expressioni

Tissue specificityi

Abundantly expressed in the skin and is expressed by keratinocytes in the epidermis. Also expressed in the brain, mammary gland, cerebellum, spinal cord and kidney. Lower levels in salivary glands, uterus, thymus, thyroid, placenta, trachea and testis. Up-regulated in ovarian carcinoma, especially late-stage serous carcinoma, compared with normal ovaries and benign adenomas (at protein level).2 Publications

Inductioni

By estrogens and glucocorticoids in a breast carcinoma cell line.1 Publication

Gene expression databases

BgeeiENSG00000169035.
CleanExiHS_KLK7.
ExpressionAtlasiP49862. baseline and differential.
GenevisibleiP49862. HS.

Organism-specific databases

HPAiCAB026342.
HPA062126.

Interactioni

Protein-protein interaction databases

BioGridi111631. 25 interactors.
IntActiP49862. 9 interactors.
STRINGi9606.ENSP00000375683.

Chemistry databases

BindingDBiP49862.

Structurei

Secondary structure

1253
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi44 – 49Combined sources6
Beta strandi52 – 61Combined sources10
Beta strandi64 – 67Combined sources4
Helixi69 – 71Combined sources3
Beta strandi76 – 81Combined sources6
Beta strandi90 – 95Combined sources6
Beta strandi97 – 100Combined sources4
Turni106 – 108Combined sources3
Beta strandi114 – 117Combined sources4
Beta strandi143 – 150Combined sources8
Beta strandi152 – 156Combined sources5
Beta strandi164 – 171Combined sources8
Helixi173 – 180Combined sources8
Helixi181 – 183Combined sources3
Beta strandi188 – 192Combined sources5
Beta strandi208 – 211Combined sources4
Beta strandi214 – 221Combined sources8
Beta strandi224 – 226Combined sources3
Beta strandi233 – 237Combined sources5
Helixi238 – 240Combined sources3
Helixi242 – 251Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QXGX-ray2.60A/B30-253[»]
2QXHX-ray2.00A30-253[»]
2QXIX-ray1.00A30-253[»]
2QXJX-ray2.10A30-253[»]
3BSQX-ray2.80A/B/C30-250[»]
ProteinModelPortaliP49862.
SMRiP49862.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49862.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 250Peptidase S1PROSITE-ProRule annotationAdd BLAST221

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP49862.
KOiK08668.
OMAiEGSHPWQ.
OrthoDBiEOG091G0DF7.
PhylomeDBiP49862.
TreeFamiTF331065.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P49862-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARSLLLPLQ ILLLSLALET AGEEAQGDKI IDGAPCARGS HPWQVALLSG
60 70 80 90 100
NQLHCGGVLV NERWVLTAAH CKMNEYTVHL GSDTLGDRRA QRIKASKSFR
110 120 130 140 150
HPGYSTQTHV NDLMLVKLNS QARLSSMVKK VRLPSRCEPP GTTCTVSGWG
160 170 180 190 200
TTTSPDVTFP SDLMCVDVKL ISPQDCTKVY KDLLENSMLC AGIPDSKKNA
210 220 230 240 250
CNGDSGGPLV CRGTLQGLVS WGTFPCGQPN DPGVYTQVCK FTKWINDTMK

KHR
Length:253
Mass (Da):27,525
Last modified:October 1, 1996 - v1
Checksum:i2D68B6B15A76A668
GO
Isoform 2 (identifier: P49862-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-72: Missing.

Show »
Length:181
Mass (Da):19,887
Checksum:i86A28A03B80C2D78
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti226C → W in AAH32005 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0135811 – 72Missing in isoform 2. 1 PublicationAdd BLAST72

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33404 mRNA. Translation: AAC37551.1.
AF166330 Genomic DNA. Translation: AAD49718.1.
AF243527 Genomic DNA. Translation: AAG33360.1.
AF332583 Genomic DNA. Translation: AAK69624.1.
AF411214 mRNA. Translation: AAN03662.1.
AF411215 mRNA. Translation: AAN03663.1.
AY601109 mRNA. Translation: AAU04540.1.
AK289660 mRNA. Translation: BAF82349.1.
CH471135 Genomic DNA. Translation: EAW71959.1.
BC032005 mRNA. Translation: AAH32005.1.
CCDSiCCDS12812.1. [P49862-1]
CCDS59414.1. [P49862-2]
PIRiA53968.
RefSeqiNP_001193982.1. NM_001207053.1. [P49862-2]
NP_001230055.1. NM_001243126.1.
NP_005037.1. NM_005046.3. [P49862-1]
NP_644806.1. NM_139277.2. [P49862-1]
UniGeneiHs.151254.

Genome annotation databases

EnsembliENST00000391807; ENSP00000375683; ENSG00000169035. [P49862-1]
ENST00000595820; ENSP00000470538; ENSG00000169035. [P49862-1]
ENST00000597707; ENSP00000469950; ENSG00000169035. [P49862-2]
GeneIDi5650.
KEGGihsa:5650.
UCSCiuc002puo.4. human. [P49862-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33404 mRNA. Translation: AAC37551.1.
AF166330 Genomic DNA. Translation: AAD49718.1.
AF243527 Genomic DNA. Translation: AAG33360.1.
AF332583 Genomic DNA. Translation: AAK69624.1.
AF411214 mRNA. Translation: AAN03662.1.
AF411215 mRNA. Translation: AAN03663.1.
AY601109 mRNA. Translation: AAU04540.1.
AK289660 mRNA. Translation: BAF82349.1.
CH471135 Genomic DNA. Translation: EAW71959.1.
BC032005 mRNA. Translation: AAH32005.1.
CCDSiCCDS12812.1. [P49862-1]
CCDS59414.1. [P49862-2]
PIRiA53968.
RefSeqiNP_001193982.1. NM_001207053.1. [P49862-2]
NP_001230055.1. NM_001243126.1.
NP_005037.1. NM_005046.3. [P49862-1]
NP_644806.1. NM_139277.2. [P49862-1]
UniGeneiHs.151254.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QXGX-ray2.60A/B30-253[»]
2QXHX-ray2.00A30-253[»]
2QXIX-ray1.00A30-253[»]
2QXJX-ray2.10A30-253[»]
3BSQX-ray2.80A/B/C30-250[»]
ProteinModelPortaliP49862.
SMRiP49862.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111631. 25 interactors.
IntActiP49862. 9 interactors.
STRINGi9606.ENSP00000375683.

Chemistry databases

BindingDBiP49862.
ChEMBLiCHEMBL2443.
GuidetoPHARMACOLOGYi2377.

Protein family/group databases

MEROPSiS01.300.

PTM databases

iPTMnetiP49862.
PhosphoSitePlusiP49862.

Polymorphism and mutation databases

BioMutaiKLK7.
DMDMi1710878.

Proteomic databases

PaxDbiP49862.
PeptideAtlasiP49862.
PRIDEiP49862.

Protocols and materials databases

DNASUi5650.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000391807; ENSP00000375683; ENSG00000169035. [P49862-1]
ENST00000595820; ENSP00000470538; ENSG00000169035. [P49862-1]
ENST00000597707; ENSP00000469950; ENSG00000169035. [P49862-2]
GeneIDi5650.
KEGGihsa:5650.
UCSCiuc002puo.4. human. [P49862-1]

Organism-specific databases

CTDi5650.
DisGeNETi5650.
GeneCardsiKLK7.
HGNCiHGNC:6368. KLK7.
HPAiCAB026342.
HPA062126.
MIMi604438. gene.
neXtProtiNX_P49862.
OpenTargetsiENSG00000169035.
PharmGKBiPA30157.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP49862.
KOiK08668.
OMAiEGSHPWQ.
OrthoDBiEOG091G0DF7.
PhylomeDBiP49862.
TreeFamiTF331065.

Enzyme and pathway databases

BioCyciZFISH:HS09870-MONOMER.
BRENDAi3.4.21.117. 2681.
ReactomeiR-HSA-1474228. Degradation of the extracellular matrix.

Miscellaneous databases

ChiTaRSiKLK7. human.
EvolutionaryTraceiP49862.
GeneWikiiKLK7.
GenomeRNAii5650.
PMAP-CutDBP49862.
PROiP49862.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169035.
CleanExiHS_KLK7.
ExpressionAtlasiP49862. baseline and differential.
GenevisibleiP49862. HS.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKLK7_HUMAN
AccessioniPrimary (citable) accession number: P49862
Secondary accession number(s): A8K0U5, Q8N5N9, Q8NFV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.