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Protein

DNA mismatch repair protein MutL

Gene

mutL

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a "molecular matchmaker", a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex (By similarity). Overexpression of mutSL partially suppresses the high spontaneous mutation frequency of a ytkD/mutM/mutY triple disruption which lacks the system required to prevent damage by oxidized guanine (8-oxo-dGTP). This suggests that MutSL also functions to repair mismatches due to oxidative stress in both growing and stationary phase cells.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciBSUB:BSU17050-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MutL
Gene namesi
Name:mutL
Ordered Locus Names:BSU17050
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene have an 80-fold increased spontaneous mutation frequency. A double mutS/mutL and a triple mutS/mutL/nth disruption has a 200 to 280-fold increased spontaneous mutation frequency.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 627627DNA mismatch repair protein MutLPRO_0000177927Add
BLAST

Proteomic databases

PaxDbiP49850.

Interactioni

Protein-protein interaction databases

IntActiP49850. 3 interactions.
STRINGi224308.Bsubs1_010100009376.

Structurei

Secondary structure

1
627
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi440 – 4456Combined sources
Turni446 – 4483Combined sources
Beta strandi449 – 4546Combined sources
Beta strandi457 – 4626Combined sources
Helixi463 – 48018Combined sources
Beta strandi486 – 49611Combined sources
Helixi501 – 5077Combined sources
Helixi509 – 5146Combined sources
Beta strandi526 – 5338Combined sources
Helixi541 – 55515Combined sources
Helixi560 – 57314Combined sources
Helixi585 – 59713Combined sources
Beta strandi601 – 6033Combined sources
Beta strandi611 – 6155Combined sources
Helixi616 – 6238Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GABX-ray2.50A/B/C/D434-627[»]
3KDGX-ray2.00A/B434-627[»]
3KDKX-ray2.26A/B434-627[»]
ProteinModelPortaliP49850.
SMRiP49850. Positions 1-326, 434-627.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49850.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DC6. Bacteria.
COG0323. LUCA.
HOGENOMiHOG000256552.
InParanoidiP49850.
KOiK03572.
OMAiQFLFINN.
PhylomeDBiP49850.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
HAMAPiMF_00149. DNA_mis_repair. 1 hit.
InterProiIPR013507. DNA_mismatch_repair_C.
IPR014762. DNA_mismatch_repair_CS.
IPR002099. DNA_mismatch_repair_fam.
IPR020667. DNA_mismatch_repair_MutL.
IPR003594. HATPase_C.
IPR014790. MutL_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF01119. DNA_mis_repair. 1 hit.
PF08676. MutL_C. 1 hit.
[Graphical view]
SMARTiSM01340. DNA_mis_repair. 1 hit.
SM00853. MutL_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00585. mutl. 1 hit.
PROSITEiPS00058. DNA_MISMATCH_REPAIR_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVIQLSDE LSNKIAAGEV VERPASVVKE LVENAIDADS TVIEIDIEEA
60 70 80 90 100
GLASIRVLDN GEGMENEDCK RAFRRHATSK IKDENDLFRV RTLGFRGEAL
110 120 130 140 150
PSIASVSHLE ITTSTGEGAG TKLVLQGGNI ISESRSSSRK GTEIVVSNLF
160 170 180 190 200
FNTPARLKYM KTVHTELGNI TDVVNRIALA HPEVSIRLRH HGKNLLQTNG
210 220 230 240 250
NGDVRHVLAA IYGTAVAKKM LPLHVSSLDF EVKGYIALPE ITRASRNYMS
260 270 280 290 300
SVVNGRYIKN FPLVKAVHEG YHTLLPIGRH PITFIEITMD PILVDVNVHP
310 320 330 340 350
SKLEVRLSKE TELHDLIRDG IKDVFKQQQL IPSAQVPKKS APAIKNEQQF
360 370 380 390 400
ITFDEKPPEK KVPEKSTAPS YSPMKLSSVV KEPVDAEEKL PPLQFDAPPI
410 420 430 440 450
VDQEQTLEVS DVSAEQPETF EQECHEEQPQ PASDRVPIMY PIGQMHGTYI
460 470 480 490 500
LAQNENGLYI IDQHAAQERI KYEYFREKVG EVEPEVQEMI VPLTFHYSTN
510 520 530 540 550
EALIIEQHKQ ELESVGVFLE SFGSNSYIVR CHPAWFPKGE EAELIEEIIQ
560 570 580 590 600
QVLDSKNIDI KKLREEAAIM MSCKGSIKAN RHLRNDEIKA LLDDLRSTSD
610 620
PFTCPHGRPI IIHHSTYEME KMFKRVM
Length:627
Mass (Da):70,431
Last modified:October 1, 1996 - v1
Checksum:i068A0509CC265343
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27343 Genomic DNA. Translation: AAB19236.1.
AL009126 Genomic DNA. Translation: CAB13578.1.
PIRiA69663.
RefSeqiNP_389587.1. NC_000964.3.
WP_003245099.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13578; CAB13578; BSU17050.
GeneIDi939455.
KEGGibsu:BSU17050.
PATRICi18975217. VBIBacSub10457_1800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27343 Genomic DNA. Translation: AAB19236.1.
AL009126 Genomic DNA. Translation: CAB13578.1.
PIRiA69663.
RefSeqiNP_389587.1. NC_000964.3.
WP_003245099.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GABX-ray2.50A/B/C/D434-627[»]
3KDGX-ray2.00A/B434-627[»]
3KDKX-ray2.26A/B434-627[»]
ProteinModelPortaliP49850.
SMRiP49850. Positions 1-326, 434-627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP49850. 3 interactions.
STRINGi224308.Bsubs1_010100009376.

Proteomic databases

PaxDbiP49850.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13578; CAB13578; BSU17050.
GeneIDi939455.
KEGGibsu:BSU17050.
PATRICi18975217. VBIBacSub10457_1800.

Phylogenomic databases

eggNOGiENOG4105DC6. Bacteria.
COG0323. LUCA.
HOGENOMiHOG000256552.
InParanoidiP49850.
KOiK03572.
OMAiQFLFINN.
PhylomeDBiP49850.

Enzyme and pathway databases

BioCyciBSUB:BSU17050-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP49850.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
HAMAPiMF_00149. DNA_mis_repair. 1 hit.
InterProiIPR013507. DNA_mismatch_repair_C.
IPR014762. DNA_mismatch_repair_CS.
IPR002099. DNA_mismatch_repair_fam.
IPR020667. DNA_mismatch_repair_MutL.
IPR003594. HATPase_C.
IPR014790. MutL_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF01119. DNA_mis_repair. 1 hit.
PF08676. MutL_C. 1 hit.
[Graphical view]
SMARTiSM01340. DNA_mis_repair. 1 hit.
SM00853. MutL_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00585. mutl. 1 hit.
PROSITEiPS00058. DNA_MISMATCH_REPAIR_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMUTL_BACSU
AccessioniPrimary (citable) accession number: P49850
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.