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Protein

Transcription initiation factor TFIID subunit 6

Gene

TAF6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors.

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000106290-MONOMER.
ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-6807505. RNA polymerase II transcribes snRNA genes.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 6
Alternative name(s):
RNA polymerase II TBP-associated factor subunit E
Transcription initiation factor TFIID 70 kDa subunit
Short name:
TAF(II)70
Short name:
TAFII-70
Short name:
TAFII70
Transcription initiation factor TFIID 80 kDa subunit
Short name:
TAF(II)80
Short name:
TAFII-80
Short name:
TAFII80
Gene namesi
Name:TAF6
Synonyms:TAF2E, TAFII70
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:11540. TAF6.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • MLL1 complex Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: HPA
  • transcription factor TFIID complex Source: UniProtKB
  • transcription factor TFTC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000106290.
PharmGKBiPA36315.

Polymorphism and mutation databases

BioMutaiTAF6.
DMDMi1729810.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001188731 – 677Transcription initiation factor TFIID subunit 6Add BLAST677

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei120PhosphothreonineCombined sources1
Modified residuei248PhosphothreonineBy similarity1
Modified residuei253PhosphotyrosineBy similarity1
Modified residuei264PhosphoserineBy similarity1
Modified residuei524Omega-N-methylarginineCombined sources1
Modified residuei626PhosphoserineBy similarity1
Modified residuei634PhosphoserineCombined sources1
Modified residuei636PhosphoserineCombined sources1
Modified residuei653PhosphoserineCombined sources1
Modified residuei660PhosphothreonineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiP49848.
MaxQBiP49848.
PaxDbiP49848.
PeptideAtlasiP49848.
PRIDEiP49848.

PTM databases

iPTMnetiP49848.
PhosphoSitePlusiP49848.

Expressioni

Gene expression databases

BgeeiENSG00000106290.
CleanExiHS_TAF6.
ExpressionAtlasiP49848. baseline and differential.
GenevisibleiP49848. HS.

Organism-specific databases

HPAiHPA006566.

Interactioni

Subunit structurei

TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TBP is not part of TFTC. Interacts directly with TBP, TAF1/TAFII250, TAF9/TAFII31 AND TAF12/TAFII20. The TAF6/TAFII70-TAF9/TAFII31 heterodimer forms an octamer complex with the TAF4B/TFII105-TAF12/TFIID20 heterodimer. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Also interacts with the GTFs, TFIIEalpha/GTF2E1 and TFIIFalpha/GTF2F1. Component of the TFTC-HAT complex.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PB1B4URF52EBI-1560206,EBI-6050669From a different organism.

Protein-protein interaction databases

BioGridi112741. 64 interactors.
DIPiDIP-773N.
IntActiP49848. 17 interactors.
MINTiMINT-1211769.
STRINGi9606.ENSP00000399982.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FURelectron microscopy8.50J/K1-677[»]
ProteinModelPortaliP49848.
SMRiP49848.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TAF6 family.Curated

Phylogenomic databases

eggNOGiKOG2549. Eukaryota.
COG5095. LUCA.
GeneTreeiENSGT00640000091486.
HOGENOMiHOG000253001.
HOVERGENiHBG012489.
InParanoidiP49848.
KOiK03131.
PhylomeDBiP49848.
TreeFamiTF313632.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR009072. Histone-fold.
IPR011442. TAF6_C.
IPR004823. TAF_TATA-bd.
[Graphical view]
PfamiPF02969. TAF. 1 hit.
PF07571. TAF6_C. 1 hit.
[Graphical view]
SMARTiSM00803. TAF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
SSF48371. SSF48371. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P49848-1) [UniParc]FASTAAdd to basket
Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEEKKLKLS NTVLPSESMK VVAESMGIAQ IQEETCQLLT DEVSYRIKEI
60 70 80 90 100
AQDALKFMHM GKRQKLTTSD IDYALKLKNV EPLYGFHAQE FIPFRFASGG
110 120 130 140 150
GRELYFYEEK EVDLSDIINT PLPRVPLDVC LKAHWLSIEG CQPAIPENPP
160 170 180 190 200
PAPKEQQKAE ATEPLKSAKP GQEEDGPLKG KGQGATTADG KGKEKKAPPL
210 220 230 240 250
LEGAPLRLKP RSIHELSVEQ QLYYKEITEA CVGSCEAKRA EALQSIATDP
260 270 280 290 300
GLYQMLPRFS TFISEGVRVN VVQNNLALLI YLMRMVKALM DNPTLYLEKY
310 320 330 340 350
VHELIPAVMT CIVSRQLCLR PDVDNHWALR DFAARLVAQI CKHFSTTTNN
360 370 380 390 400
IQSRITKTFT KSWVDEKTPW TTRYGSIAGL AELGHDVIKT LILPRLQQEG
410 420 430 440 450
ERIRSVLDGP VLSNIDRIGA DHVQSLLLKH CAPVLAKLRP PPDNQDAYRA
460 470 480 490 500
EFGSLGPLLC SQVVKARAQA ALQAQQVNRT TLTITQPRPT LTLSQAPQPG
510 520 530 540 550
PRTPGLLKVP GSIALPVQTL VSARAAAPPQ PSPPPTKFIV MSSSSSAPST
560 570 580 590 600
QQVLSLSTSA PGSGSTTTSP VTTTVPSVQP IVKLVSTATT APPSTAPSGP
610 620 630 640 650
GSVQKYIVVS LPPTGEGKGG PTSHPSPVPP PASSPSPLSG SALCGGKQEA
660 670
GDSPPPAPGT PKANGSQPNS GSPQPAP
Length:677
Mass (Da):72,668
Last modified:October 1, 1996 - v1
Checksum:i06C6BFA563580823
GO
Isoform 2 (identifier: P49848-2) [UniParc]FASTAAdd to basket
Also known as: Gamma

The sequence of this isoform differs from the canonical sequence as follows:
     477-486: Missing.

Show »
Length:667
Mass (Da):71,540
Checksum:i85488E83976800FC
GO
Isoform 3 (identifier: P49848-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTKRRLQTITACLQLREGSPSLHRGLHPSREEKRDSRM

Note: No experimental confirmation available.
Show »
Length:714
Mass (Da):76,995
Checksum:i1A1E41AB1BC47388
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti196K → E in CAH10485 (PubMed:17974005).Curated1
Sequence conflicti417R → W in CAH10485 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01434936C → S.1 PublicationCorresponds to variant rs4134897dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0437091M → MTKRRLQTITACLQLREGSP SLHRGLHPSREEKRDSRM in isoform 3. 1 Publication1
Alternative sequenceiVSP_037476477 – 486Missing in isoform 2. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25444 mRNA. Translation: AAA63643.1.
U31659 mRNA. Translation: AAA84390.1.
AK300005 mRNA. Translation: BAG61823.1.
CR627390 mRNA. Translation: CAH10485.1.
AY149894 Genomic DNA. Translation: AAN10295.1.
CH236956 Genomic DNA. Translation: EAL23851.1.
CH236956 Genomic DNA. Translation: EAL23852.1.
CH471091 Genomic DNA. Translation: EAW76589.1.
CH471091 Genomic DNA. Translation: EAW76591.1.
CH471091 Genomic DNA. Translation: EAW76592.1.
BC018115 mRNA. Translation: AAH18115.1.
CCDSiCCDS55135.1. [P49848-3]
CCDS5686.1. [P49848-1]
RefSeqiNP_001177344.1. NM_001190415.1. [P49848-3]
NP_005632.1. NM_005641.3. [P49848-1]
NP_647476.1. NM_139315.2. [P49848-1]
XP_006716163.1. XM_006716100.2. [P49848-1]
UniGeneiHs.489309.

Genome annotation databases

EnsembliENST00000344095; ENSP00000344537; ENSG00000106290. [P49848-1]
ENST00000437822; ENSP00000399982; ENSG00000106290. [P49848-3]
ENST00000452041; ENSP00000416396; ENSG00000106290. [P49848-1]
ENST00000453269; ENSP00000389575; ENSG00000106290. [P49848-1]
GeneIDi6878.
KEGGihsa:6878.
UCSCiuc003uti.4. human. [P49848-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25444 mRNA. Translation: AAA63643.1.
U31659 mRNA. Translation: AAA84390.1.
AK300005 mRNA. Translation: BAG61823.1.
CR627390 mRNA. Translation: CAH10485.1.
AY149894 Genomic DNA. Translation: AAN10295.1.
CH236956 Genomic DNA. Translation: EAL23851.1.
CH236956 Genomic DNA. Translation: EAL23852.1.
CH471091 Genomic DNA. Translation: EAW76589.1.
CH471091 Genomic DNA. Translation: EAW76591.1.
CH471091 Genomic DNA. Translation: EAW76592.1.
BC018115 mRNA. Translation: AAH18115.1.
CCDSiCCDS55135.1. [P49848-3]
CCDS5686.1. [P49848-1]
RefSeqiNP_001177344.1. NM_001190415.1. [P49848-3]
NP_005632.1. NM_005641.3. [P49848-1]
NP_647476.1. NM_139315.2. [P49848-1]
XP_006716163.1. XM_006716100.2. [P49848-1]
UniGeneiHs.489309.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FURelectron microscopy8.50J/K1-677[»]
ProteinModelPortaliP49848.
SMRiP49848.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112741. 64 interactors.
DIPiDIP-773N.
IntActiP49848. 17 interactors.
MINTiMINT-1211769.
STRINGi9606.ENSP00000399982.

PTM databases

iPTMnetiP49848.
PhosphoSitePlusiP49848.

Polymorphism and mutation databases

BioMutaiTAF6.
DMDMi1729810.

Proteomic databases

EPDiP49848.
MaxQBiP49848.
PaxDbiP49848.
PeptideAtlasiP49848.
PRIDEiP49848.

Protocols and materials databases

DNASUi6878.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000344095; ENSP00000344537; ENSG00000106290. [P49848-1]
ENST00000437822; ENSP00000399982; ENSG00000106290. [P49848-3]
ENST00000452041; ENSP00000416396; ENSG00000106290. [P49848-1]
ENST00000453269; ENSP00000389575; ENSG00000106290. [P49848-1]
GeneIDi6878.
KEGGihsa:6878.
UCSCiuc003uti.4. human. [P49848-1]

Organism-specific databases

CTDi6878.
GeneCardsiTAF6.
HGNCiHGNC:11540. TAF6.
HPAiHPA006566.
MIMi602955. gene.
neXtProtiNX_P49848.
OpenTargetsiENSG00000106290.
PharmGKBiPA36315.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2549. Eukaryota.
COG5095. LUCA.
GeneTreeiENSGT00640000091486.
HOGENOMiHOG000253001.
HOVERGENiHBG012489.
InParanoidiP49848.
KOiK03131.
PhylomeDBiP49848.
TreeFamiTF313632.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000106290-MONOMER.
ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-6807505. RNA polymerase II transcribes snRNA genes.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

ChiTaRSiTAF6. human.
GeneWikiiTAF6.
GenomeRNAii6878.
PROiP49848.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000106290.
CleanExiHS_TAF6.
ExpressionAtlasiP49848. baseline and differential.
GenevisibleiP49848. HS.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR009072. Histone-fold.
IPR011442. TAF6_C.
IPR004823. TAF_TATA-bd.
[Graphical view]
PfamiPF02969. TAF. 1 hit.
PF07571. TAF6_C. 1 hit.
[Graphical view]
SMARTiSM00803. TAF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
SSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTAF6_HUMAN
AccessioniPrimary (citable) accession number: P49848
Secondary accession number(s): A4D2B2
, A4D2B3, B4DT11, D6W5U2, Q6AI29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 168 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.