P49847 (TAF6_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor TFIID subunit 6

Alternative name(s):
TAFII-60
TAFII-62
Transcription initiation factor TFIID 62 kDa subunit
Short name=p62

Gene names

Name:	Taf6
Synonyms:	Taf60
ORF Names:	CG32211

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	606 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Ref.5
Subunit structure	Belongs to the TFIID complex which is composed of TATA binding protein (Tbp) and a number of TBP-associated factors (TAFs). E(y)1 and Taf6 exist as a heterotetramer. Interacts with Taf1. Ref.5 Ref.7
Subcellular location	Nucleus.
Sequence similarities	Belongs to the TAF6 family.
Sequence caution	The sequence AAA16536.1 differs from that shown. Reason: Erroneous initiation. The sequence AAC46480.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	RNA polymerase II transcriptional preinitiation complex assembly Inferred from direct assay PubMed 16895980PubMed 7664336. Source: GOC neurogenesis Inferred from mutant phenotype PubMed 21549331. Source: FlyBase regulation of sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Cellular_component	SAGA complex Inferred from direct assay PubMed 16980620. Source: FlyBase transcription factor TFIID complex Inferred from direct assay PubMed 7664336. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 606

606

Transcription initiation factor TFIID subunit 6

PRO_0000118877

Amino acid modifications

Modified residue

546

1

Phosphoserine Ref.6

Modified residue

547

1

Phosphoserine Ref.6

Modified residue

550

1

Phosphoserine Ref.6

Experimental info

Sequence conflict

3

1

G → K AA sequence Ref.4

Sequence conflict

101

1

F → L in AAA16536. Ref.5

Sequence conflict

582

1

D → E in AAC46480. Ref.4

Sequence conflict

582

1

D → E in AAA16536. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

606

Helix Strand Turn

Details...

Helix

23 – 32

10

Helix

40 – 67

28

Beta strand

71 – 73

3

Helix

75 – 82

8

Sequences

	Sequence		Length	Mass (Da)
	P49847 [UniParc]. Last modified January 27, 2003. Version 2. Checksum: 9D93C3496A23C0A3	FASTA	606	65,654
	10 20 30 40 50 60 MSGKPSKPSS PSSSMLYGSS ISAESMKVIA ESIGVGSLSD DAAKELAEDV SIKLKRIVQD 70 80 90 100 110 120 AAKFMNHAKR QKLSVRDIDM SLKVRNVEPQ YGFVAKDFIP FRFASGGGRE LHFTEDKEID 130 140 150 160 170 180 LGEITSTNSV KIPLDLTLRS HWFVVEGVQP TVPENPPPLS KDSQLLDSVN PVIKMDQGLN 190 200 210 220 230 240 KDAAGKPTTG KIHKLKNVET IHVKQLATHE LSVEQQLYYK EITEACVGSD EPRRGEALQS 250 260 270 280 290 300 LGSDPGLHEM LPRMCTFIAE GVKVNVVQNN LALLIYLMRM VRALLDNPSL FLEKYLHELI 310 320 330 340 350 360 PSVMTCIVSK QLCMRPELDN HWALRDFASR LMAQICKNFN TLTNNLQTRV TRIFSKALQN 370 380 390 400 410 420 DKTHLSSLYG SIAGLSELGG EVIKVFIIPR LKFISERIEP HLLGTSISNT DKTAAGHIRA 430 440 450 460 470 480 MLQKCCPPIL RQMRSAPDTA EDYKNDFGFL GPSLCQAVVK VRNAPASSIV TLSSNTINTA 490 500 510 520 530 540 PITSAAQTAT TIGRVSMPTT QRQGSPGVSS LPQIRAIQAN QPAQKFVIVT QNSPQQGQAK 550 560 570 580 590 600 VVRRGSSPHS VVLSAASNAA SASNSNSSSS GSLLAAAQRS SDNVCVIAGS EAPAVDGITV QSFRAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[4]	"Molecular cloning of Drosophila TFIID subunits." Kokubo T., Gong D.-W., Wootton J.C., Horikoshi M., Roeder R.G., Nakatani Y. Nature 367:484-487(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-606, PARTIAL PROTEIN SEQUENCE.
[5]	"Cloning and expression of Drosophila TAFII60 and human TAFII70 reveal conserved interactions with other subunits of TFIID." Weinzierl R.O., Ruppert S., Dynlacht B.D., Tanese N., Tjian R. EMBO J. 12:5303-5309(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-606, PARTIAL PROTEIN SEQUENCE, FUNCTION, INTERACTION WITH TAF1. Strain: Oregon-R.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-546; SER-547 AND SER-550, MASS SPECTROMETRY. Tissue: Embryo.
[7]	"Structural similarity between TAFs and the heterotetrameric core of the histone octamer." Xie X., Kokubo T., Cohen S.L., Mirza U.A., Hoffmann A., Chait B.T., Roeder R.G., Nakatani Y., Burley S.K. Nature 380:316-322(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 15-84, INTERACTION WITH E(Y)1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE014296 Genomic DNA. Translation: AAF49139.2.
AY051702 mRNA. Translation: AAK93126.1.
U06459 mRNA. Translation: AAC46480.1. Different initiation.
L25443 mRNA. Translation: AAA16536.1. Different initiation.

RefSeq

NP_524161.2. NM_079437.4.

UniGene

Dm.2802.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TAF	X-ray	2.00	B	15-84	[»]

ProteinModelPortal

P49847.

SMR

P49847. Positions 15-83, 207-387.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-772N.
DIP-778N.

IntAct

P49847. 2 interactions.

MINT

MINT-755268.

Proteomic databases

PaxDb

P49847.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0074941; FBpp0074709; FBgn0010417.

GeneID

40134.

KEGG

dme:Dmel_CG32211.

Organism-specific databases

CTD

6878.

FlyBase

FBgn0010417. Taf6.

Phylogenomic databases

eggNOG

COG5095.

GeneTree

ENSGT00640000091486.

InParanoid

P49847.

KO

K03131.

OMA

KQLATHE.

OrthoDB

EOG434TN9.

PhylomeDB

P49847.

Gene expression databases

Bgee

P49847.

GermOnline

CG32211. Drosophila melanogaster.

Family and domain databases

Gene3D

1.10.20.10. 1 hit.
1.25.10.10. 1 hit.

InterPro

IPR011989. ARM-like.
IPR011442. DUF1546.
IPR009072. Histone-fold.
IPR004823. TAF_TATA-bd.
[Graphical view]

Pfam

PF07571. DUF1546. 1 hit.
PF02969. TAF. 1 hit.
[Graphical view]

SMART

SM00803. TAF. 1 hit.
[Graphical view]

SUPFAM

SSF47113. Histone-fold. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P49847.

GenomeRNAi

40134.

NextBio

817166.

Entry information

Entry name

TAF6_DROME

Accession

Primary (citable) accession number: P49847
Secondary accession number(s): Q961B6, Q9VW16

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 27, 2003
Last modified:	May 1, 2013
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project