Reviewed, UniProtKB/Swiss-Prot P49823 (ODPA_CANFA)

Names and origin

Protein names

Recommended name:
    Pyruvate dehydrogenase E1 component subunit alpha, somatic form
    EC=1.2.4.1
Alternative name(s):
    PDHE1-A type I

Gene names

Name:

PDHA1

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	13 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Catalytic activity	Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.
Cofactor	Thiamine pyrophosphate.
Enzyme regulation	E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit By similarity.
Subunit structure	Tetramer of 2 alpha and 2 beta subunits.
Subcellular location	Mitochondrion matrix.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Mitochondrion
Ligand	Pyruvate Thiamine pyrophosphate
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	pyruvate dehydrogenase (acetyl-transferring) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›13	›13	Pyruvate dehydrogenase E1 component subunit alpha, somatic form		PRO_0000162214
Experimental info
	Non-terminal residue	13	1

Sequences

	Sequence		Length	Mass (Da)
	P49823-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C97EEBF844085B19	FASTA	13	1,513
	10 XXDATFEIKK XDL

References

[1]	"HSC-2DPAGE and the two-dimensional gel electrophoresis database of dog heart proteins." Dunn M.J., Corbett J.M., Wheeler C.H. Electrophoresis 18:2795-2802(1997) [PubMed: 9504812] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Heart.

Cross-references

3D structure databases
ModBase	Search...
2-D gel databases
HSC-2DPAGE	P49823.
Phylogenomic databases
HOVERGEN	P49823.
Enzyme and pathway databases
BRENDA	1.2.4.1. 463.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ODPA_CANFA

Accession

Primary (citable) accession number: P49823

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 20, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)