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Protein

Serum albumin

Gene

ALB

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi27 – 271CopperBy similarity
Metal bindingi91 – 911ZincBy similarity
Metal bindingi123 – 1231ZincBy similarity
Metal bindingi271 – 2711ZincBy similarity
Metal bindingi273 – 2731ZincBy similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. drug binding Source: UniProtKB
  3. fatty acid binding Source: UniProtKB
  4. metal ion binding Source: UniProtKB-KW
  5. pyridoxal phosphate binding Source: UniProtKB
  6. toxic substance binding Source: UniProtKB

GO - Biological processi

  1. cellular response to starvation Source: UniProtKB
  2. hemolysis by symbiont of host erythrocytes Source: UniProtKB
  3. maintenance of mitochondrion location Source: UniProtKB
  4. negative regulation of apoptotic process Source: UniProtKB
  5. transport Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Copper, Lipid-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Serum albumin
Alternative name(s):
Allergen: Can f 3
Gene namesi
Name:ALB
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: InterPro
  2. protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.2 Publications

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei176. Can f 3.
3171. Can f 3.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Propeptidei19 – 224PRO_0000001059
Chaini25 – 608584Serum albuminPRO_0000001060Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi77 ↔ 86PROSITE-ProRule annotation
Modified residuei82 – 821PhosphoserineBy similarity
Modified residuei89 – 891PhosphoserineBy similarity
Disulfide bondi99 ↔ 115PROSITE-ProRule annotation
Disulfide bondi114 ↔ 125PROSITE-ProRule annotation
Disulfide bondi148 ↔ 193PROSITE-ProRule annotation
Disulfide bondi192 ↔ 201PROSITE-ProRule annotation
Disulfide bondi224 ↔ 270PROSITE-ProRule annotation
Modified residuei229 – 2291N6-succinyllysineBy similarity
Disulfide bondi269 ↔ 277PROSITE-ProRule annotation
Disulfide bondi289 ↔ 303PROSITE-ProRule annotation
Disulfide bondi302 ↔ 313PROSITE-ProRule annotation
Disulfide bondi340 ↔ 385PROSITE-ProRule annotation
Disulfide bondi384 ↔ 393PROSITE-ProRule annotation
Disulfide bondi416 ↔ 462PROSITE-ProRule annotation
Modified residuei443 – 4431PhosphoserineBy similarity
Modified residuei444 – 4441PhosphothreonineBy similarity
Modified residuei446 – 4461PhosphothreonineBy similarity
Modified residuei460 – 4601N6-succinyllysineBy similarity
Disulfide bondi461 ↔ 472PROSITE-ProRule annotation
Disulfide bondi485 ↔ 501PROSITE-ProRule annotation
Disulfide bondi500 ↔ 511PROSITE-ProRule annotation
Modified residuei513 – 5131PhosphoserineBy similarity
Disulfide bondi538 ↔ 583PROSITE-ProRule annotation
Disulfide bondi582 ↔ 591PROSITE-ProRule annotation
Modified residuei588 – 5881N6-succinyllysineBy similarity

Post-translational modificationi

Phosphorylation sites are present in the extracellular medium.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP49822.
PRIDEiP49822.

2D gel databases

UCD-2DPAGEP49822.

Expressioni

Tissue specificityi

Plasma.

Interactioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000032573.

Structurei

3D structure databases

ProteinModelPortaliP49822.
SMRiP49822. Positions 29-585.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 210192Albumin 1PROSITE-ProRule annotationAdd
BLAST
Domaini211 – 403193Albumin 2PROSITE-ProRule annotationAdd
BLAST
Domaini404 – 601198Albumin 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation
Contains 3 albumin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG45992.
HOGENOMiHOG000293137.
HOVERGENiHBG004207.
InParanoidiP49822.
KOiK16141.

Family and domain databases

InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamiPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSiPR00802. SERUMALBUMIN.
SMARTiSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 3 hits.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWVTFISLF FLFSSAYSRG LVRREAYKSE IAHRYNDLGE EHFRGLVLVA
60 70 80 90 100
FSQYLQQCPF EDHVKLAKEV TEFAKACAAE ESGANCDKSL HTLFGDKLCT
110 120 130 140 150
VASLRDKYGD MADCCEKQEP DRNECFLAHK DDNPGFPPLV APEPDALCAA
160 170 180 190 200
FQDNEQLFLG KYLYEIARRH PYFYAPELLY YAQQYKGVFA ECCQAADKAA
210 220 230 240 250
CLGPKIEALR EKVLLSSAKE RFKCASLQKF GDRAFKAWSV ARLSQRFPKA
260 270 280 290 300
DFAEISKVVT DLTKVHKECC HGDLLECADD RADLAKYMCE NQDSISTKLK
310 320 330 340 350
ECCDKPVLEK SQCLAEVERD ELPGDLPSLA ADFVEDKEVC KNYQEAKDVF
360 370 380 390 400
LGTFLYEYAR RHPEYSVSLL LRLAKEYEAT LEKCCATDDP PTCYAKVLDE
410 420 430 440 450
FKPLVDEPQN LVKTNCELFE KLGEYGFQNA LLVRYTKKAP QVSTPTLVEV
460 470 480 490 500
SRKLGKVGTK CCKKPESERM SCAEDFLSVV LNRLCVLHEK TPVSERVTKC
510 520 530 540 550
CSESLVNRRP CFSGLEVDET YVPKEFNAET FTFHADLCTL PEAEKQVKKQ
560 570 580 590 600
TALVELLKHK PKATDEQLKT VMGDFGAFVE KCCAAENKEG CFSEEGPKLV

AAAQAALV
Length:608
Mass (Da):68,605
Last modified:December 21, 2004 - v3
Checksum:i3DB012FF7C979CF3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 2626MKWVT…VRREA → MDT in CAA76841 (PubMed:10669848).CuratedAdd
BLAST
Sequence conflicti146 – 1461A → R in CAA76841 (PubMed:10669848).Curated
Sequence conflicti206 – 2061I → T in CAA76841 (PubMed:10669848).Curated
Sequence conflicti349 – 3491V → A in CAA76841 (PubMed:10669848).Curated
Sequence conflicti359 – 3591A → S in CAA76841 (PubMed:10669848).Curated
Sequence conflicti359 – 3591A → S in AAB30434 (PubMed:7512102).Curated
Sequence conflicti448 – 4481V → VV in AAB30434 (PubMed:7512102).Curated
Sequence conflicti474 – 4741E → D in CAA76841 (PubMed:10669848).Curated
Sequence conflicti474 – 4741E → D in AAB30434 (PubMed:7512102).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133489 mRNA. Translation: CAB64867.1.
Y17737 mRNA. Translation: CAA76841.1.
AB090854 mRNA. Translation: BAC10663.1.
S72946 mRNA. Translation: AAB30434.1.
PIRiS29749.
RefSeqiNP_001003026.1. NM_001003026.1.
UniGeneiCfa.5991.

Genome annotation databases

GeneIDi403550.
KEGGicfa:403550.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133489 mRNA. Translation: CAB64867.1.
Y17737 mRNA. Translation: CAA76841.1.
AB090854 mRNA. Translation: BAC10663.1.
S72946 mRNA. Translation: AAB30434.1.
PIRiS29749.
RefSeqiNP_001003026.1. NM_001003026.1.
UniGeneiCfa.5991.

3D structure databases

ProteinModelPortaliP49822.
SMRiP49822. Positions 29-585.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000032573.

Protein family/group databases

Allergomei176. Can f 3.
3171. Can f 3.0101.

2D gel databases

UCD-2DPAGEP49822.

Proteomic databases

PaxDbiP49822.
PRIDEiP49822.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi403550.
KEGGicfa:403550.

Organism-specific databases

CTDi213.

Phylogenomic databases

eggNOGiNOG45992.
HOGENOMiHOG000293137.
HOVERGENiHBG004207.
InParanoidiP49822.
KOiK16141.

Miscellaneous databases

NextBioi20817059.

Family and domain databases

InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR021177. Serum_albumin/AFP.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
[Graphical view]
PfamiPF00273. Serum_albumin. 3 hits.
[Graphical view]
PIRSFiPIRSF002520. Serum_albumin_subgroup. 1 hit.
PRINTSiPR00802. SERUMALBUMIN.
SMARTiSM00103. ALBUMIN. 3 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 3 hits.
PS51438. ALBUMIN_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Hilger C.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Beagle.
    Tissue: Liver.
  2. "Escherichia coli expression and purification of recombinant dog albumin, a cross-reactive animal allergen."
    Pandjaitan B., Swoboda I., Brandejsky-Pichler F., Rumpold H., Valenta R., Spitzauer S.
    J. Allergy Clin. Immunol. 105:279-285(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN.
    Tissue: Liver.
  3. "Isolation of a cDNA encoding canine serum albumin."
    Miyake M., Okazaki M., Iwabuchi S.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Beagle.
    Tissue: Liver.
  4. "Isolation, amino acid sequence and copper(II)-binding properties of peptide (1-24) of dog serum albumin."
    Dixon J.W., Sarkar B.
    J. Biol. Chem. 249:5872-5877(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-48.
  5. "HSC-2DPAGE and the two-dimensional gel electrophoresis database of dog heart proteins."
    Dunn M.J., Corbett J.M., Wheeler C.H.
    Electrophoresis 18:2795-2802(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-38.
    Tissue: Heart.
  6. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 215-478, ALLERGEN.
    Tissue: Salivary gland.

Entry informationi

Entry nameiALBU_CANFA
AccessioniPrimary (citable) accession number: P49822
Secondary accession number(s): O77705, Q9TSZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 21, 2004
Last modified: March 4, 2015
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.