Tuberin - Rattus norvegicus (Rat)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Tuberin

Alternative name(s):
Tuberous sclerosis 2 protein homolog

Gene names

Name:

Tsc2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1809 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1 By similarity. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling By similarity. Specifically stimulates the intrinsic GTPase activity of the Ras-related protein RAP1A and RAB5. Suggesting a possible mechanism for its role in regulating cellular growth.
Subunit structure	Interacts with TSC1 and HERC1; the interaction with TSC1 stabilizes TSC2 and prevents the interaction with HERC1. May also interact with the adapter molecule RABEP1. The final complex contains TSC2 and RABEP1 linked to RAB5. Interacts with HSPA1 and HSPA8 By similarity. Interacts with DAPK1 By similarity. Ref.3
Tissue specificity	CNS, uterus, heart, skeletal muscle, kidney and spleen.
Post-translational modification	Phosphorylation at Ser-1388, Ser-1420 or Ser-1422 does not affect interaction with TSC1. Phosphorylation at Ser-939 and Thr-1466 by PKB/AKT1 is induced by growth factor stimulation. Phosphorylation by AMPK activates it and leads to negatively regulates the mTORC1 complex. Phosphorylated at Ser-1800 by RPS6KA1; phosphorylation inhibits TSC2 ability to suppress mTORC1 signaling By similarity. Phosphorylated by DAPK1 By similarity. Ubiquitinated by the DCX(FBXW5) E3 ubiquitin-protein ligase complex, leading to its subsequent degradation By similarity.
Involvement in disease	A germline insertion in Tsc2 is the cause of the Eker rat model of inherited cancer susceptibility. Gives rise to a spectrum of epithelial and nonepithelial neoplasms.
Sequence similarities	Contains 1 Rap-GAP domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Disease	Tumor suppressor
Molecular function	GTPase activation
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	acute-phase response Inferred from mutant phenotype Ref.2. Source: RGD cell projection organization Inferred from mutant phenotype PubMed 16286931. Source: MGI establishment of protein localization Inferred from mutant phenotype PubMed 15093748. Source: RGD heart development Inferred from electronic annotation. Source: Compara insulin-like growth factor receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of MAP kinase activity Inferred from mutant phenotype PubMed 14612383. Source: RGD negative regulation of TOR signaling cascade Inferred from direct assay PubMed 12869586. Source: RGD negative regulation of Wnt receptor signaling pathway Inferred from direct assay PubMed 12511557. Source: RGD negative regulation of cell size Inferred from mutant phenotype PubMed 16286931. Source: MGI negative regulation of epithelial cell proliferation Inferred from direct assay PubMed 14612383. Source: RGD negative regulation of phosphatidylinositol 3-kinase cascade Inferred from electronic annotation. Source: Compara negative regulation of protein kinase B signaling cascade Inferred from electronic annotation. Source: Compara neural tube closure Inferred from electronic annotation. Source: Compara phosphatidylinositol 3-kinase cascade Inferred from electronic annotation. Source: Compara positive chemotaxis Inferred from electronic annotation. Source: Compara protein heterooligomerization Inferred from direct assay PubMed 17379185. Source: RGD protein homooligomerization Inferred from direct assay PubMed 17379185. Source: RGD protein import into nucleus Inferred from electronic annotation. Source: Compara protein kinase B signaling cascade Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of cell cycle Inferred from mutant phenotype Ref.2. Source: RGD regulation of endocytosis Inferred from electronic annotation. Source: Compara regulation of insulin receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome response to hypoxia Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara TSC1-TSC2 complex Inferred from Biological aspect of Ancestor. Source: RefGenome caveola Inferred from direct assay PubMed 15093748. Source: RGD cytoskeletal part Inferred from direct assay PubMed 12147258. Source: RGD dendrite Inferred from direct assay PubMed 15525761. Source: RGD growth cone Inferred from direct assay PubMed 14559897. Source: RGD neuronal cell body Inferred from direct assay PubMed 15525761. Source: RGD nucleus Inferred from Biological aspect of Ancestor. Source: RefGenome perinuclear region of cytoplasm Inferred from direct assay PubMed 14559897. Source: RGD
Molecular_function	Rab GTPase activator activity Traceable author statement PubMed 12045200. Source: RGD phosphatase binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select] Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P49816-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P49816-2) The sequence of this isoform differs from the canonical sequence as follows: 947-989: Missing.

Isoform 3 (identifier: P49816-3) The sequence of this isoform differs from the canonical sequence as follows: 1272-1294: Missing.

Isoform 4 (identifier: P49816-4) The sequence of this isoform differs from the canonical sequence as follows: 947-989: Missing. 1272-1294: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1809

1809

Tuberin

PRO_0000065656

Regions

Domain

1533 – 1760

228

Rap-GAP

Amino acid modifications

Modified residue

927

1

Phosphothreonine By similarity

Modified residue

939

1

Phosphoserine; by PKB/AKT1 By similarity

Modified residue

981

1

Phosphoserine By similarity

Modified residue

1132

1

Phosphoserine By similarity

Modified residue

1155

1

Phosphoserine By similarity

Modified residue

1340

1

Phosphoserine By similarity

Modified residue

1341

1

Phosphoserine By similarity

Modified residue

1349

1

Phosphoserine By similarity

Modified residue

1366

1

Phosphoserine By similarity

Modified residue

1389

1

Phosphoserine By similarity

Modified residue

1413

1

Phosphoserine By similarity

Modified residue

1420

1

Phosphoserine By similarity

Modified residue

1422

1

Phosphoserine By similarity

Modified residue

1452

1

Phosphoserine; by AMPK By similarity

Modified residue

1456

1

Phosphoserine By similarity

Modified residue

1466

1

Phosphothreonine; by PKB/AKT1 By similarity

Modified residue

1800

1

Phosphoserine; by RPS6KA1 By similarity

Natural variations

Alternative sequence

947 – 989

43

Missing in isoform 2 and isoform 4.

VSP_004481

Alternative sequence

1272 – 1294

23

Missing in isoform 3 and isoform 4.

VSP_004482

Experimental info

Mutagenesis

314

1

N → S in chemically induced renal carcinoma. Ref.4

Mutagenesis

713

1

L → R in chemically induced renal carcinoma. Ref.4

Sequence conflict

932

1

K → S in BAA08914. Ref.2

Sequence conflict

1514

1

C → F in BAA08914. Ref.2

Sequence conflict

1730

1

R → S in BAA08914. Ref.2

Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6190BEFB45272664 Show »	FASTA	1,809	201,278
10 20 30 40 50 60 MAKPTSKDSG LKEKFKILLG LGTSRPNPRC AEGKQTEFII TAEILRELSG ECGLNNRIRM 70 80 90 100 110 120 IGQICDVAKT KKLEEHAVEA LWKAVSDLLQ PERPPEARHA VLALLKAIVQ GQGDRLGVLR 130 140 150 160 170 180 ALFFKVIKDY PSNEDLHERL EVFKALTDNG RHITYLEEEL AEFVLQWMDV GLSSEFLLVL 190 200 210 220 230 240 VNLVKFNSCY LDEYIAPMVH MICLLCIRTV SSVDIEVSLQ VLDAVVCYNC LPAESLPLFI 250 260 270 280 290 300 ITLCRTVNVK ELCEPCWKLM RNLLGTHLGH SAIYNMCRIM ENRSYMEDAP LLRGAVFFVG 310 320 330 340 350 360 MALWGAHRLY SLKNSPTSVL PSFYEAMTCP NEVVSYEIVL SITRLIKKYR KELQAVTWDI 370 380 390 400 410 420 LLDIIERLLQ QLQNLDSPEL RTIVHDLLTT VEELCDQNEF HGSQERYYEL VESYADQRPE 430 440 450 460 470 480 SSLLNLITYR AQSIHPAKDG WIQNLQLLME RFFRNECRSA VRIKVLDVLS FVLLINRQFY 490 500 510 520 530 540 EEELINSVVI SQLSHIPEDK DHQVRKLATQ LLVDLAEGCH THHFNSLLDI IEKVMARSLS 550 560 570 580 590 600 PPLELEERDL AVYSASLEDV KTAVLGLLVI LQTKLYTLPA SHATRVYETL ISHIQLHYKH 610 620 630 640 650 660 GYSLPIASSI RLQAFDFLLL LRADSLHRLG LPNKDGVVRF SPYCLCDCAE LDRASEKKAS 670 680 690 700 710 720 GPLSPPTGPP SPVPTGPAVR LGHLPYSLLF RVLLQCLKQE TDWKVLKLVL SKLPESLRYK 730 740 750 760 770 780 VLIFTSPCSV DQLSSALCSM LSAPKTLERL RGTPEGFSRT DLHLAVVPVL TALISYHNYL 790 800 810 820 830 840 DKTRQREMVY CLEQGLIYRC ASQCVVALAI CSVEMPDIII KALPVLVVKL THISATASMA 850 860 870 880 890 900 IPLLEFLSTL ARLPHLYRNF AAEQYASVFA ISLPYTNPSK FNQYIVCLAH HVIAMWFIRC 910 920 930 940 950 960 RLPFRKDFVP YITKGLRSNV LLSFDDTPEK DKFRARSTSL NERPKSLRIA RAPKQGLNNS 970 980 990 1000 1010 1020 PPVKEFKESC AAEAFRCRSI SVSEHVVRSR IQTSLTSASL GSADENSMAQ ADDNLKNLHL 1030 1040 1050 1060 1070 1080 ELTETCLDMM ARYVFSNFTA VPKRSPVGEF LLAGGRTKTW LVGNKLVTVT TSVGTGTRSL 1090 1100 1110 1120 1130 1140 LGLDSGDLQG GSASSSDPGT HVRQTKEAPA KLESQAGQQV SRGARDRVRS MSGGHGLRVG 1150 1160 1170 1180 1190 1200 VLDTSAPYTP GGPASLGAQA APAARPEKPC AGAQLPAAEK ANLAAYVPLL TQGWAEILVR 1210 1220 1230 1240 1250 1260 RPTGNTSWLM SLENPLSPFS SDINNMPLQE LSNALMAAER FKEHRDTALY KSLSVPAAGT 1270 1280 1290 1300 1310 1320 AKPPTLPRSN TVASFSSLYQ PSCQGQLHRS VSWADSAVVL EEGSPGEAHV PVEPPELEDF 1330 1340 1350 1360 1370 1380 EAALGTDRHC QRPDAYSRSS SASSQEEKSH LEELAAGGIP IERAISSEGA RPTVDLSFQP 1390 1400 1410 1420 1430 1440 SQPLSKSSSS PELQTLQDIL GDLGDKTDIG RLSPEAKVRS QSGILDGEAA TWSAPGEESR 1450 1460 1470 1480 1490 1500 ITVPPEGPLP SSSPRSPSGL RPRGYTISDS APSRRGKRVE RDNFKSRTAA SSAEKVPGIN 1510 1520 1530 1540 1550 1560 PSFVFLQLYH SPFCGDESNK PILLPNESFE RSVQLLDQIP SYDTHKIAVL YVGEGQSSSE 1570 1580 1590 1600 1610 1620 LAILSNEHGS YRYTEFLTGL GRLIELKDCQ PDKVYLGGLD VCGEDGQFTY CWHDDIMQAV 1630 1640 1650 1660 1670 1680 FHIATLMPTK DVDKHRCDKK RHLGNDFVSI IYNDSGEDFK LGTIKGQFNF VHVIITPLDY 1690 1700 1710 1720 1730 1740 KCNLLTLQCR KDMEGLVDTS VAKIVSDRNL SFVARQMALH ANMASQVHHR RSNPTDIYPS 1750 1760 1770 1780 1790 1800 KWIARLRHIK RLRQRIREEV HYSNPSLPLM HPPAHTKVPA QAPTEATPTY ETGQRKRLIS SVDDFTEFV « Hide
Isoform 2 [UniParc]. Checksum: 85B44025626BC659 Show »	FASTA	1,766	196,527
10 20 30 40 50 60 MAKPTSKDSG LKEKFKILLG LGTSRPNPRC AEGKQTEFII TAEILRELSG ECGLNNRIRM 70 80 90 100 110 120 IGQICDVAKT KKLEEHAVEA LWKAVSDLLQ PERPPEARHA VLALLKAIVQ GQGDRLGVLR 130 140 150 160 170 180 ALFFKVIKDY PSNEDLHERL EVFKALTDNG RHITYLEEEL AEFVLQWMDV GLSSEFLLVL 190 200 210 220 230 240 VNLVKFNSCY LDEYIAPMVH MICLLCIRTV SSVDIEVSLQ VLDAVVCYNC LPAESLPLFI 250 260 270 280 290 300 ITLCRTVNVK ELCEPCWKLM RNLLGTHLGH SAIYNMCRIM ENRSYMEDAP LLRGAVFFVG 310 320 330 340 350 360 MALWGAHRLY SLKNSPTSVL PSFYEAMTCP NEVVSYEIVL SITRLIKKYR KELQAVTWDI 370 380 390 400 410 420 LLDIIERLLQ QLQNLDSPEL RTIVHDLLTT VEELCDQNEF HGSQERYYEL VESYADQRPE 430 440 450 460 470 480 SSLLNLITYR AQSIHPAKDG WIQNLQLLME RFFRNECRSA VRIKVLDVLS FVLLINRQFY 490 500 510 520 530 540 EEELINSVVI SQLSHIPEDK DHQVRKLATQ LLVDLAEGCH THHFNSLLDI IEKVMARSLS 550 560 570 580 590 600 PPLELEERDL AVYSASLEDV KTAVLGLLVI LQTKLYTLPA SHATRVYETL ISHIQLHYKH 610 620 630 640 650 660 GYSLPIASSI RLQAFDFLLL LRADSLHRLG LPNKDGVVRF SPYCLCDCAE LDRASEKKAS 670 680 690 700 710 720 GPLSPPTGPP SPVPTGPAVR LGHLPYSLLF RVLLQCLKQE TDWKVLKLVL SKLPESLRYK 730 740 750 760 770 780 VLIFTSPCSV DQLSSALCSM LSAPKTLERL RGTPEGFSRT DLHLAVVPVL TALISYHNYL 790 800 810 820 830 840 DKTRQREMVY CLEQGLIYRC ASQCVVALAI CSVEMPDIII KALPVLVVKL THISATASMA 850 860 870 880 890 900 IPLLEFLSTL ARLPHLYRNF AAEQYASVFA ISLPYTNPSK FNQYIVCLAH HVIAMWFIRC 910 920 930 940 950 960 RLPFRKDFVP YITKGLRSNV LLSFDDTPEK DKFRARSTSL NERPKSRIQT SLTSASLGSA 970 980 990 1000 1010 1020 DENSMAQADD NLKNLHLELT ETCLDMMARY VFSNFTAVPK RSPVGEFLLA GGRTKTWLVG 1030 1040 1050 1060 1070 1080 NKLVTVTTSV GTGTRSLLGL DSGDLQGGSA SSSDPGTHVR QTKEAPAKLE SQAGQQVSRG 1090 1100 1110 1120 1130 1140 ARDRVRSMSG GHGLRVGVLD TSAPYTPGGP ASLGAQAAPA ARPEKPCAGA QLPAAEKANL 1150 1160 1170 1180 1190 1200 AAYVPLLTQG WAEILVRRPT GNTSWLMSLE NPLSPFSSDI NNMPLQELSN ALMAAERFKE 1210 1220 1230 1240 1250 1260 HRDTALYKSL SVPAAGTAKP PTLPRSNTVA SFSSLYQPSC QGQLHRSVSW ADSAVVLEEG 1270 1280 1290 1300 1310 1320 SPGEAHVPVE PPELEDFEAA LGTDRHCQRP DAYSRSSSAS SQEEKSHLEE LAAGGIPIER 1330 1340 1350 1360 1370 1380 AISSEGARPT VDLSFQPSQP LSKSSSSPEL QTLQDILGDL GDKTDIGRLS PEAKVRSQSG 1390 1400 1410 1420 1430 1440 ILDGEAATWS APGEESRITV PPEGPLPSSS PRSPSGLRPR GYTISDSAPS RRGKRVERDN 1450 1460 1470 1480 1490 1500 FKSRTAASSA EKVPGINPSF VFLQLYHSPF CGDESNKPIL LPNESFERSV QLLDQIPSYD 1510 1520 1530 1540 1550 1560 THKIAVLYVG EGQSSSELAI LSNEHGSYRY TEFLTGLGRL IELKDCQPDK VYLGGLDVCG 1570 1580 1590 1600 1610 1620 EDGQFTYCWH DDIMQAVFHI ATLMPTKDVD KHRCDKKRHL GNDFVSIIYN DSGEDFKLGT 1630 1640 1650 1660 1670 1680 IKGQFNFVHV IITPLDYKCN LLTLQCRKDM EGLVDTSVAK IVSDRNLSFV ARQMALHANM 1690 1700 1710 1720 1730 1740 ASQVHHRRSN PTDIYPSKWI ARLRHIKRLR QRIREEVHYS NPSLPLMHPP AHTKVPAQAP 1750 1760 TEATPTYETG QRKRLISSVD DFTEFV « Hide
Isoform 3 [UniParc]. Checksum: 8D02C6EA34616F36 Show »	FASTA	1,786	198,757
10 20 30 40 50 60 MAKPTSKDSG LKEKFKILLG LGTSRPNPRC AEGKQTEFII TAEILRELSG ECGLNNRIRM 70 80 90 100 110 120 IGQICDVAKT KKLEEHAVEA LWKAVSDLLQ PERPPEARHA VLALLKAIVQ GQGDRLGVLR 130 140 150 160 170 180 ALFFKVIKDY PSNEDLHERL EVFKALTDNG RHITYLEEEL AEFVLQWMDV GLSSEFLLVL 190 200 210 220 230 240 VNLVKFNSCY LDEYIAPMVH MICLLCIRTV SSVDIEVSLQ VLDAVVCYNC LPAESLPLFI 250 260 270 280 290 300 ITLCRTVNVK ELCEPCWKLM RNLLGTHLGH SAIYNMCRIM ENRSYMEDAP LLRGAVFFVG 310 320 330 340 350 360 MALWGAHRLY SLKNSPTSVL PSFYEAMTCP NEVVSYEIVL SITRLIKKYR KELQAVTWDI 370 380 390 400 410 420 LLDIIERLLQ QLQNLDSPEL RTIVHDLLTT VEELCDQNEF HGSQERYYEL VESYADQRPE 430 440 450 460 470 480 SSLLNLITYR AQSIHPAKDG WIQNLQLLME RFFRNECRSA VRIKVLDVLS FVLLINRQFY 490 500 510 520 530 540 EEELINSVVI SQLSHIPEDK DHQVRKLATQ LLVDLAEGCH THHFNSLLDI IEKVMARSLS 550 560 570 580 590 600 PPLELEERDL AVYSASLEDV KTAVLGLLVI LQTKLYTLPA SHATRVYETL ISHIQLHYKH 610 620 630 640 650 660 GYSLPIASSI RLQAFDFLLL LRADSLHRLG LPNKDGVVRF SPYCLCDCAE LDRASEKKAS 670 680 690 700 710 720 GPLSPPTGPP SPVPTGPAVR LGHLPYSLLF RVLLQCLKQE TDWKVLKLVL SKLPESLRYK 730 740 750 760 770 780 VLIFTSPCSV DQLSSALCSM LSAPKTLERL RGTPEGFSRT DLHLAVVPVL TALISYHNYL 790 800 810 820 830 840 DKTRQREMVY CLEQGLIYRC ASQCVVALAI CSVEMPDIII KALPVLVVKL THISATASMA 850 860 870 880 890 900 IPLLEFLSTL ARLPHLYRNF AAEQYASVFA ISLPYTNPSK FNQYIVCLAH HVIAMWFIRC 910 920 930 940 950 960 RLPFRKDFVP YITKGLRSNV LLSFDDTPEK DKFRARSTSL NERPKSLRIA RAPKQGLNNS 970 980 990 1000 1010 1020 PPVKEFKESC AAEAFRCRSI SVSEHVVRSR IQTSLTSASL GSADENSMAQ ADDNLKNLHL 1030 1040 1050 1060 1070 1080 ELTETCLDMM ARYVFSNFTA VPKRSPVGEF LLAGGRTKTW LVGNKLVTVT TSVGTGTRSL 1090 1100 1110 1120 1130 1140 LGLDSGDLQG GSASSSDPGT HVRQTKEAPA KLESQAGQQV SRGARDRVRS MSGGHGLRVG 1150 1160 1170 1180 1190 1200 VLDTSAPYTP GGPASLGAQA APAARPEKPC AGAQLPAAEK ANLAAYVPLL TQGWAEILVR 1210 1220 1230 1240 1250 1260 RPTGNTSWLM SLENPLSPFS SDINNMPLQE LSNALMAAER FKEHRDTALY KSLSVPAAGT 1270 1280 1290 1300 1310 1320 AKPPTLPRSN TDSAVVLEEG SPGEAHVPVE PPELEDFEAA LGTDRHCQRP DAYSRSSSAS 1330 1340 1350 1360 1370 1380 SQEEKSHLEE LAAGGIPIER AISSEGARPT VDLSFQPSQP LSKSSSSPEL QTLQDILGDL 1390 1400 1410 1420 1430 1440 GDKTDIGRLS PEAKVRSQSG ILDGEAATWS APGEESRITV PPEGPLPSSS PRSPSGLRPR 1450 1460 1470 1480 1490 1500 GYTISDSAPS RRGKRVERDN FKSRTAASSA EKVPGINPSF VFLQLYHSPF CGDESNKPIL 1510 1520 1530 1540 1550 1560 LPNESFERSV QLLDQIPSYD THKIAVLYVG EGQSSSELAI LSNEHGSYRY TEFLTGLGRL 1570 1580 1590 1600 1610 1620 IELKDCQPDK VYLGGLDVCG EDGQFTYCWH DDIMQAVFHI ATLMPTKDVD KHRCDKKRHL 1630 1640 1650 1660 1670 1680 GNDFVSIIYN DSGEDFKLGT IKGQFNFVHV IITPLDYKCN LLTLQCRKDM EGLVDTSVAK 1690 1700 1710 1720 1730 1740 IVSDRNLSFV ARQMALHANM ASQVHHRRSN PTDIYPSKWI ARLRHIKRLR QRIREEVHYS 1750 1760 1770 1780 NPSLPLMHPP AHTKVPAQAP TEATPTYETG QRKRLISSVD DFTEFV « Hide
Isoform 4 [UniParc]. Checksum: F923B79ADBF45D10 Show »	FASTA	1,743	194,006
10 20 30 40 50 60 MAKPTSKDSG LKEKFKILLG LGTSRPNPRC AEGKQTEFII TAEILRELSG ECGLNNRIRM 70 80 90 100 110 120 IGQICDVAKT KKLEEHAVEA LWKAVSDLLQ PERPPEARHA VLALLKAIVQ GQGDRLGVLR 130 140 150 160 170 180 ALFFKVIKDY PSNEDLHERL EVFKALTDNG RHITYLEEEL AEFVLQWMDV GLSSEFLLVL 190 200 210 220 230 240 VNLVKFNSCY LDEYIAPMVH MICLLCIRTV SSVDIEVSLQ VLDAVVCYNC LPAESLPLFI 250 260 270 280 290 300 ITLCRTVNVK ELCEPCWKLM RNLLGTHLGH SAIYNMCRIM ENRSYMEDAP LLRGAVFFVG 310 320 330 340 350 360 MALWGAHRLY SLKNSPTSVL PSFYEAMTCP NEVVSYEIVL SITRLIKKYR KELQAVTWDI 370 380 390 400 410 420 LLDIIERLLQ QLQNLDSPEL RTIVHDLLTT VEELCDQNEF HGSQERYYEL VESYADQRPE 430 440 450 460 470 480 SSLLNLITYR AQSIHPAKDG WIQNLQLLME RFFRNECRSA VRIKVLDVLS FVLLINRQFY 490 500 510 520 530 540 EEELINSVVI SQLSHIPEDK DHQVRKLATQ LLVDLAEGCH THHFNSLLDI IEKVMARSLS 550 560 570 580 590 600 PPLELEERDL AVYSASLEDV KTAVLGLLVI LQTKLYTLPA SHATRVYETL ISHIQLHYKH 610 620 630 640 650 660 GYSLPIASSI RLQAFDFLLL LRADSLHRLG LPNKDGVVRF SPYCLCDCAE LDRASEKKAS 670 680 690 700 710 720 GPLSPPTGPP SPVPTGPAVR LGHLPYSLLF RVLLQCLKQE TDWKVLKLVL SKLPESLRYK 730 740 750 760 770 780 VLIFTSPCSV DQLSSALCSM LSAPKTLERL RGTPEGFSRT DLHLAVVPVL TALISYHNYL 790 800 810 820 830 840 DKTRQREMVY CLEQGLIYRC ASQCVVALAI CSVEMPDIII KALPVLVVKL THISATASMA 850 860 870 880 890 900 IPLLEFLSTL ARLPHLYRNF AAEQYASVFA ISLPYTNPSK FNQYIVCLAH HVIAMWFIRC 910 920 930 940 950 960 RLPFRKDFVP YITKGLRSNV LLSFDDTPEK DKFRARSTSL NERPKSRIQT SLTSASLGSA 970 980 990 1000 1010 1020 DENSMAQADD NLKNLHLELT ETCLDMMARY VFSNFTAVPK RSPVGEFLLA GGRTKTWLVG 1030 1040 1050 1060 1070 1080 NKLVTVTTSV GTGTRSLLGL DSGDLQGGSA SSSDPGTHVR QTKEAPAKLE SQAGQQVSRG 1090 1100 1110 1120 1130 1140 ARDRVRSMSG GHGLRVGVLD TSAPYTPGGP ASLGAQAAPA ARPEKPCAGA QLPAAEKANL 1150 1160 1170 1180 1190 1200 AAYVPLLTQG WAEILVRRPT GNTSWLMSLE NPLSPFSSDI NNMPLQELSN ALMAAERFKE 1210 1220 1230 1240 1250 1260 HRDTALYKSL SVPAAGTAKP PTLPRSNTDS AVVLEEGSPG EAHVPVEPPE LEDFEAALGT 1270 1280 1290 1300 1310 1320 DRHCQRPDAY SRSSSASSQE EKSHLEELAA GGIPIERAIS SEGARPTVDL SFQPSQPLSK 1330 1340 1350 1360 1370 1380 SSSSPELQTL QDILGDLGDK TDIGRLSPEA KVRSQSGILD GEAATWSAPG EESRITVPPE 1390 1400 1410 1420 1430 1440 GPLPSSSPRS PSGLRPRGYT ISDSAPSRRG KRVERDNFKS RTAASSAEKV PGINPSFVFL 1450 1460 1470 1480 1490 1500 QLYHSPFCGD ESNKPILLPN ESFERSVQLL DQIPSYDTHK IAVLYVGEGQ SSSELAILSN 1510 1520 1530 1540 1550 1560 EHGSYRYTEF LTGLGRLIEL KDCQPDKVYL GGLDVCGEDG QFTYCWHDDI MQAVFHIATL 1570 1580 1590 1600 1610 1620 MPTKDVDKHR CDKKRHLGND FVSIIYNDSG EDFKLGTIKG QFNFVHVIIT PLDYKCNLLT 1630 1640 1650 1660 1670 1680 LQCRKDMEGL VDTSVAKIVS DRNLSFVARQ MALHANMASQ VHHRRSNPTD IYPSKWIARL 1690 1700 1710 1720 1730 1740 RHIKRLRQRI REEVHYSNPS LPLMHPPAHT KVPAQAPTEA TPTYETGQRK RLISSVDDFT EFV « Hide

References

[1]	"Identification of tuberous sclerosis 2 messenger RNA splice variants that are conserved and differentially expressed in rat and human tissues." Xiao G.-H., Jin F., Yeung R.S. Cell Growth Differ. 6:1185-1191(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4). Strain: Wistar. Tissue: Kidney.
[2]	"cDNA structure, alternative splicing and exon-intron organization of the predisposing tuberous sclerosis (Tsc2) gene of the Eker rat model." Kobayashi T., Nishizawa M., Hirayama Y., Kobayashi E., Hino O. Nucleic Acids Res. 23:2608-2613(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: Long Evans. Tissue: Brain and Kidney.
[3]	"The tuberous sclerosis 2 gene product, tuberin, functions as a Rab5 GTPase activating protein (GAP) in modulating endocytosis." Xiao G.-H., Shoarinejad F., Jin F., Golemis E.A., Yeung R.S. J. Biol. Chem. 272:6097-6100(1997) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RAB5.
[4]	"Isolation and characterization of a rat homologue of the human tuberous sclerosis 1 gene (Tsc1) and analysis of its mutations in rat renal carcinomas." Satake N., Kobayashi T., Kobayashi E., Izumi K., Hino O. Cancer Res. 59:849-855(1999) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF ASN-314 AND LEU-713.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U24150 mRNA. Translation: AAC52289.1. D50413 mRNA. Translation: BAA08914.1.
IPI	IPI00230778. IPI00230779. IPI00230780. IPI00323980.
PIR	S57329.
RefSeq	NP_036812.2. NM_012680.3.
UniGene	Rn.5875.
3D structure databases
ProteinModelPortal	P49816.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49816. 2 interactions.
MINT	MINT-4783802.
STRING	10116.ENSRNOP00000016221.
PTM databases
PhosphoSite	P49816.
Proteomic databases
PaxDb	P49816.
PRIDE	P49816.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000016221; ENSRNOP00000016221; ENSRNOG00000011375.
GeneID	24855.
KEGG	rno:24855.
UCSC	RGD:3908. rat.
Organism-specific databases
CTD	7249.
RGD	3908. Tsc2.
Phylogenomic databases
eggNOG	NOG290929.
GeneTree	ENSGT00390000017866.
HOGENOM	HOG000045987.
HOVERGEN	HBG018005.
InParanoid	P49816.
KO	K07207.
OrthoDB	EOG4N8R3W.
Gene expression databases
ArrayExpress	P49816.
Genevestigator	P49816.
GermOnline	ENSRNOG00000011375. Rattus norvegicus.
Family and domain databases
Gene3D	1.25.10.10. 3 hits.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000331. Rap_GAP_dom. IPR003913. Tuberin. IPR018515. Tuberin-type_domain. IPR027107. Tuberin/Ral-act_asu. IPR024584. Tuberin_N. [Graphical view]
PANTHER	PTHR10063. PTHR10063. 1 hit.
Pfam	PF11864. DUF3384. 1 hit. PF02145. Rap_GAP. 1 hit. PF03542. Tuberin. 1 hit. [Graphical view]
PRINTS	PR01431. TUBERIN.
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50085. RAPGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	604654.

Entry information

Entry name

TSC2_RAT

Accession

Primary (citable) accession number: P49816

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents