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P49814 (MDH_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase
EC=1.1.1.37
Alternative name(s):
Vegetative protein 69
Short name=VEG69
Gene names
Name:mdh
Synonyms:citH
Ordered Locus Names:BSU29120
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1 Ref.5
Chain2 – 312311Malate dehydrogenase HAMAP MF_00487
PRO_0000113434

Regions

Nucleotide binding12 – 176NAD By similarity
Nucleotide binding123 – 1253NAD By similarity

Sites

Active site1801Proton acceptor By similarity
Binding site361NAD By similarity
Binding site871Substrate By similarity
Binding site931Substrate By similarity
Binding site1001NAD By similarity
Binding site1251Substrate By similarity
Binding site1561Substrate By similarity

Amino acid modifications

Modified residue1491Phosphoserine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P49814 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 656BA5BF5AA2D0CD

FASTA31233,644
        10         20         30         40         50         60 
MGNTRKKVSV IGAGFTGATT AFLIAQKELA DVVLVDIPQL ENPTKGKALD MLEASPVQGF 

        70         80         90        100        110        120 
DAKITGTSNY EDTAGSDIVV ITAGIARKPG MSRDDLVSTN EKIMRSVTQE IVKYSPDSII 

       130        140        150        160        170        180 
VVLTNPVDAM TYAVYKESGF PKERVIGQSG VLDTARFRTF VAEELNLSVK DVTGFVLGGH 

       190        200        210        220        230        240 
GDDMVPLVRY SYAGGIPLET LIPKERIDAI VERTRKGGGE IVNLLGNGSA YYAPAASLTE 

       250        260        270        280        290        300 
MVEAILKDQR RVLPTIAYLE GEYGYEGIYL GVPTIVGGNG LEQIIELELT DYERAQLNKS 

       310 
VESVKNVMKV LS

« Hide

References

« Hide 'large scale' references
[1]"Identification of two distinct Bacillus subtilis citrate synthase genes."
Jin S., Sonenshein A.L.
J. Bacteriol. 176:4669-4679(1994) [PubMed: 8045898] [Abstract]
Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-312.
Strain: 168 / SMY.
[2]"A Bacillus subtilis malate dehydrogenase gene."
Jin S., de Jesus-Berrios M., Sonenshein A.L.
J. Bacteriol. 178:560-563(1996) [PubMed: 8550482] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / SMY.
[3]"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
Microbiology 143:3431-3441(1997) [PubMed: 9387221] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[4]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[5]"First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis."
Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M.
Electrophoresis 18:1451-1463(1997) [PubMed: 9298659] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-16.
Strain: 168 / IS58.
[6]"The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis."
Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R., Mann M.
Mol. Cell. Proteomics 6:697-707(2007) [PubMed: 17218307] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149, MASS SPECTROMETRY.
Strain: 168.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U05257 Genomic DNA. Translation: AAA96343.1.
AF008220 Genomic DNA. Translation: AAC00347.1.
AL009126 Genomic DNA. Translation: CAB14872.1.
PIRI40383.
RefSeqNP_390790.1. NC_000964.3.

3D structure databases

ProteinModelPortalP49814.
SMRP49814. Positions 5-311.
ModBaseSearch...

PTM databases

PhosSiteP49814.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000004067; EBBACP00000004067; EBBACG00000004059.
GeneID937385.
GenomeReviewsGene locus BSU29120 in contig AL009126_GR.
KEGGbsu:BSU29120.
NMPDRfig|224308.1.peg.2915.
PATRIC18977718. VBIBacSub10457_3047.

Organism-specific databases

GenoListBSU29120. [Micado]

Phylogenomic databases

GeneTreeEBGT00070000032140.
HOGENOMHBG566126.
OMAEIVVITA.
PhylomeDBP49814.
ProtClustDBPRK06223.

Enzyme and pathway databases

BioCycBSUB:BSU29120-MONOMER.

Family and domain databases

HAMAPMF_00487. Malate_dehydrog_3.
[Tree]
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00024.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMsTIGR01763. MalateDH_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_BACSU
AccessionPrimary (citable) accession number: P49814
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: January 25, 2012
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents