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P49814

- MDH_BACSU

UniProt

P49814 - MDH_BACSU

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Protein

Malate dehydrogenase

Gene

mdh

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361NADBy similarity
Binding sitei87 – 871SubstrateBy similarity
Binding sitei93 – 931SubstrateBy similarity
Binding sitei100 – 1001NADBy similarity
Binding sitei125 – 1251SubstrateBy similarity
Binding sitei156 – 1561SubstrateBy similarity
Active sitei180 – 1801Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176NADBy similarity
Nucleotide bindingi123 – 1253NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciBSUB:BSU29120-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Alternative name(s):
Vegetative protein 69
Short name:
VEG69
Gene namesi
Name:mdh
Synonyms:citH
Ordered Locus Names:BSU29120
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU29120. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 312311Malate dehydrogenasePRO_0000113434Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei149 – 1491Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49814.

PTM databases

PhosSiteiP0802202.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
icdP391263EBI-7827708,EBI-7829570

Protein-protein interaction databases

IntActiP49814. 2 interactions.
MINTiMINT-8365164.
STRINGi224308.BSU29120.

Structurei

3D structure databases

ProteinModelPortaliP49814.
SMRiP49814. Positions 5-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.Curated

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
InParanoidiP49814.
KOiK00024.
OMAiYGQNDIC.
OrthoDBiEOG6091FG.
PhylomeDBiP49814.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49814-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGNTRKKVSV IGAGFTGATT AFLIAQKELA DVVLVDIPQL ENPTKGKALD
60 70 80 90 100
MLEASPVQGF DAKITGTSNY EDTAGSDIVV ITAGIARKPG MSRDDLVSTN
110 120 130 140 150
EKIMRSVTQE IVKYSPDSII VVLTNPVDAM TYAVYKESGF PKERVIGQSG
160 170 180 190 200
VLDTARFRTF VAEELNLSVK DVTGFVLGGH GDDMVPLVRY SYAGGIPLET
210 220 230 240 250
LIPKERIDAI VERTRKGGGE IVNLLGNGSA YYAPAASLTE MVEAILKDQR
260 270 280 290 300
RVLPTIAYLE GEYGYEGIYL GVPTIVGGNG LEQIIELELT DYERAQLNKS
310
VESVKNVMKV LS
Length:312
Mass (Da):33,644
Last modified:January 23, 2007 - v3
Checksum:i656BA5BF5AA2D0CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05257 Genomic DNA. Translation: AAA96343.1.
AF008220 Genomic DNA. Translation: AAC00347.1.
AL009126 Genomic DNA. Translation: CAB14872.1.
PIRiI40383.
RefSeqiNP_390790.1. NC_000964.3.
WP_003229437.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB14872; CAB14872; BSU29120.
GeneIDi937385.
KEGGibsu:BSU29120.
PATRICi18977718. VBIBacSub10457_3047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05257 Genomic DNA. Translation: AAA96343.1 .
AF008220 Genomic DNA. Translation: AAC00347.1 .
AL009126 Genomic DNA. Translation: CAB14872.1 .
PIRi I40383.
RefSeqi NP_390790.1. NC_000964.3.
WP_003229437.1. NZ_CM000487.1.

3D structure databases

ProteinModelPortali P49814.
SMRi P49814. Positions 5-311.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P49814. 2 interactions.
MINTi MINT-8365164.
STRINGi 224308.BSU29120.

PTM databases

PhosSitei P0802202.

Proteomic databases

PaxDbi P49814.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB14872 ; CAB14872 ; BSU29120 .
GeneIDi 937385.
KEGGi bsu:BSU29120.
PATRICi 18977718. VBIBacSub10457_3047.

Organism-specific databases

GenoListi BSU29120. [Micado ]

Phylogenomic databases

eggNOGi COG0039.
HOGENOMi HOG000213794.
InParanoidi P49814.
KOi K00024.
OMAi YGQNDIC.
OrthoDBi EOG6091FG.
PhylomeDBi P49814.

Enzyme and pathway databases

BioCyci BSUB:BSU29120-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_00487. Malate_dehydrog_3.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
PRINTSi PR00086. LLDHDRGNASE.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01763. MalateDH_bact. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of two distinct Bacillus subtilis citrate synthase genes."
    Jin S., Sonenshein A.L.
    J. Bacteriol. 176:4669-4679(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-312.
    Strain: 168 / SMY.
  2. "A Bacillus subtilis malate dehydrogenase gene."
    Jin S., de Jesus-Berrios M., Sonenshein A.L.
    J. Bacteriol. 178:560-563(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / SMY.
  3. "Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
    Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
    Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  5. "First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis."
    Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M.
    Electrophoresis 18:1451-1463(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-16.
    Strain: 168 / IS58.
  6. "The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis."
    Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R., Mann M.
    Mol. Cell. Proteomics 6:697-707(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: 168.

Entry informationi

Entry nameiMDH_BACSU
AccessioniPrimary (citable) accession number: P49814
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3