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P49805 (RGS9_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulator of G-protein signaling 9

Short name=RGS9
Gene names
Name:Rgs9
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length677 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction By similarity.

Subunit structure

Heterodimer with Gbeta5. Interacts with RGS7BP, leading to regulate the subcellular location of the heterodimer formed with Gbeta5. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (RGS9-1), Gbeta5 (GNB5) and RGS9BP Probable.

Subcellular location

Membrane; Peripheral membrane protein. Note: Targeted to the membrane via its interaction with RGS9BP By similarity.

Tissue specificity

Expressed in the central nervous system. Isoform RGS9L is found in striatum, hypothalamus and nucleus accumbens while isoform RGS9S is expressed in retina and pineal gland.

Sequence similarities

Contains 1 DEP domain.

Contains 1 G protein gamma domain.

Contains 1 RGS domain.

Ontologies

Keywords
   Biological processSensory transduction
Vision
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   Molecular functionSignal transduction inhibitor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdopamine receptor signaling pathway

Inferred from mutant phenotype PubMed 15534226. Source: RGD

intracellular signal transduction

Inferred from electronic annotation. Source: InterPro

nervous system development

Inferred from expression pattern PubMed 16153714. Source: RGD

positive regulation of GTPase activity

Inferred from direct assay PubMed 15534226. Source: RGD

regulation of G-protein coupled receptor protein signaling pathway

Inferred from direct assay Ref.1. Source: RGD

response to estrogen

Inferred from expression pattern PubMed 15640770. Source: RGD

termination of G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

visual perception

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 15110994. Source: RGD

heterotrimeric G-protein complex

Inferred from electronic annotation. Source: InterPro

nucleus

Inferred from direct assay PubMed 15110994. Source: RGD

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionGTPase activator activity

Inferred from direct assay Ref.1. Source: RGD

protein complex binding

Inferred from direct assay PubMed 16510730. Source: RGD

signal transducer activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform RGS9L (identifier: P49805-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform RGS9S (identifier: P49805-2)

The sequence of this isoform differs from the canonical sequence as follows:
     467-677: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 677677Regulator of G-protein signaling 9
PRO_0000204205

Regions

Domain30 – 10576DEP
Domain219 – 28062G protein gamma
Domain295 – 416122RGS

Natural variations

Alternative sequence467 – 677211Missing in isoform RGS9S.
VSP_005680

Sequences

Sequence LengthMass (Da)Tools
Isoform RGS9L [UniParc].

Last modified May 30, 2000. Version 3.
Checksum: 39F403E3D3C37A67

FASTA67777,131
        10         20         30         40         50         60 
MTIRHQGQQY RPRMAFLQKI EALVKDMQNP ETGVRMQNQR VLVTSVPHAM TGGDVLQWII 

        70         80         90        100        110        120 
QRLWISNLEA QNLGNFIVKY GYIYPLQDPK NLVLKPDSSL YRFQTPYFWP TQQWPAEDTD 

       130        140        150        160        170        180 
YAIYLAKRNI KKKGILEEYE KENYDFLNKK INYKWDFVIM QAKEQYRTGK ERNKADRYAL 

       190        200        210        220        230        240 
DCQEKAYWLV HRSPPGMNNV LDYGLDRVTN PNEVKKQTVT AVRKEIMYYQ QALMRSTVKS 

       250        260        270        280        290        300 
SVSLGGIVKY SEQFSSNDAI MSGCLPSNPW ITDDTQFWDL NAKLVEVPTK MRVERWAFNF 

       310        320        330        340        350        360 
SELIRDPKGR QSFQYFLKKE FSGENLGFWE ACEDLKYGDQ SKVKEKAEEI YKLFLAPGAR 

       370        380        390        400        410        420 
RWINIDGKTM DITVKGLRHP HRYVLDAAQT HIYMLMKKDS YARYLKSPIY KEMLAKAIEP 

       430        440        450        460        470        480 
QETTKRSSTL PFMRRHLRSS PSPVILRQLE EEERAREAAN TVDITQPGQH LAPSPHLAVY 

       490        500        510        520        530        540 
TGTCVPPSPS SPFSPSCRSP RKPFPSPSRF IRRPSIAICP SPIRVALEGS SGLEGKGEAS 

       550        560        570        580        590        600 
WSGANPGPPV TESIETSVDR SRPHSQPRAP LKARAALSLG RFLRRGCLAS PVFARLSPKC 

       610        620        630        640        650        660 
PSVSHGKVQP LGDMGQQLPR LKPKKVANFF QIKMEMPTDS GPCLMDSDDP GAGESGDQTT 

       670 
EKEVICPWES LAEGKAG 

« Hide

Isoform RGS9S [UniParc].

Checksum: E2C79A3F16EEB6ED
Show »

FASTA46654,808

References

[1]"Molecular characterization of human and rat RGS9L, a novel splice variant enriched in dopamine target regions, and chromosomal localization of the RGS9 gene."
Granneman J.G., Zhai Y., Zhu Z., Bannon M.J., Burchett S.A., Schmidt C.J., Andrade R., Cooper J.
Mol. Pharmacol. 54:687-694(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Strain: Sprague-Dawley.
Tissue: Hypothalamus.
[2]"A long form of rat regulator of G-protein signaling 9 (RGS9)."
Zhou J., Moroi K., Homma S., Usui H., Nishiyama M., Kimura S.
Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM RGS9L).
Tissue: Brain.
[3]"RGS9: a regulator of G-protein signalling with specific expression in rat and mouse striatum."
Thomas E.A., Danielson P.E., Sutcliffe J.G.
J. Neurosci. Res. 52:118-124(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 234-677 (ISOFORM RGS9L).
Strain: Sprague-Dawley.
Tissue: Cerebellum, Corpus striatum and Hippocampus.
[4]"EGL-10 regulates G protein signaling in the C. elegans nervous system and shares a conserved domain with many mammalian proteins."
Koelle M.R., Horvitz H.R.
Cell 84:115-125(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 330-395.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF071475 mRNA. Translation: AAC64039.1.
AB019145 mRNA. Translation: BAA34051.1.
AF038006 mRNA. Translation: AAC01959.1.
U32433 mRNA. Translation: AAC52370.1.
RefSeqNP_062097.1. NM_019224.2. [P49805-1]
UniGeneRn.10903.

3D structure databases

ProteinModelPortalP49805.
SMRP49805. Positions 7-421.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000005076.

Proteomic databases

PaxDbP49805.
PRIDEP49805.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000005076; ENSRNOP00000005076; ENSRNOG00000003800. [P49805-1]
GeneID29481.
KEGGrno:29481.

Organism-specific databases

CTD8787.
RGD3572. Rgs9.

Phylogenomic databases

eggNOGNOG327614.
GeneTreeENSGT00700000104259.
HOGENOMHOG000220864.
HOVERGENHBG007404.
InParanoidP49805.
KOK13765.
OMALVKDMQN.
OrthoDBEOG7BZVS5.
PhylomeDBP49805.

Gene expression databases

GenevestigatorP49805.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
1.10.196.10. 1 hit.
4.10.260.10. 1 hit.
InterProIPR000591. DEP_dom.
IPR015898. G-protein_gamma-like_dom.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00610. DEP. 1 hit.
PF00631. G-gamma. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR01301. RGSPROTEIN.
SMARTSM00049. DEP. 1 hit.
SM00224. GGL. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF48670. SSF48670. 1 hit.
PROSITEPS50186. DEP. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio609332.
PROP49805.

Entry information

Entry nameRGS9_RAT
AccessionPrimary (citable) accession number: P49805
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: May 14, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families