Reviewed,
UniProtKB/Swiss-Prot P49795 (RGS19_HUMAN)
Last modified
November 3, 2009.
Version 100.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Regulator of G-protein signaling 19 Short name=RGS19 Alternative name(s): G-alpha-interacting protein Short name=GAIP | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 217 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, with the order G(i)a3 > G(i)a1 > G(o)a >> G(z)a/G(i)a2. Activity on G(z)-alpha is inhibited by phosphorylation and palmitoylation of the G-protein. |
| Subunit structure | Interacts with GIPC PDZ domain. |
| Subcellular location | |
| Tissue specificity | Highest expression in lung. Placenta, liver and heart also express high levels of GAIP. |
| Post-translational modification | Fatty acylated. Heavily palmitoylated in the cysteine string motif. Ref.8 |
| Sequence similarities | Contains 1 RGS domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Autophagy |
| Cellular component | Membrane |
| Molecular function | Signal transduction inhibitor |
| PTM | Lipoprotein Palmitate Phosphoprotein |
| Technical term | 3D-structure Complete proteome |
| Gene Ontology (GO) | |
| Biological process | G-protein coupled receptor protein signaling pathway Ref.1 Traceable author statement. Source: ProtInc autophagyInferred from electronic annotation. Source: UniProtKB-KW negative regulation of signal transductionInferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transductionTraceable author statement. Source: ProtInc |
| Cellular component | Golgi apparatus Traceable author statement. Source: ProtInc anchored to membraneInferred from electronic annotation. Source: UniProtKB-SubCell membrane fractionTraceable author statement. Source: ProtInc |
| Molecular function | protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB signal transducer activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Gnai3 | P08753 | 3 | EBI-874907,EBI-874897 | From a different organism. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 217 | 217 | Regulator of G-protein signaling 19 | PRO_0000204229 | ||||||||||||||||||||||||||||
Regions | ||||||||||||||||||||||||||||||||
| Domain | 90 – 206 | 117 | RGS | |||||||||||||||||||||||||||||
| Region | 207 – 217 | 11 | Interaction with GIPC | |||||||||||||||||||||||||||||
| Compositional bias | 39 – 49 | 11 | Poly-Cys | |||||||||||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||||||||||
| Modified residue | 24 | 1 | Phosphoserine By similarity | |||||||||||||||||||||||||||||
| Modified residue | 97 | 1 | Phosphoserine Ref.9 | |||||||||||||||||||||||||||||
| Modified residue | 151 | 1 | Phosphoserine; by MAPK1 and MAPK3 Ref.12 | |||||||||||||||||||||||||||||
Experimental info | ||||||||||||||||||||||||||||||||
| Mutagenesis | 151 | 1 | S → A: Diminishes gap activity towards G(i)-alpha3 and autophagy in colon cancer cells. Ref.12 | |||||||||||||||||||||||||||||
| Sequence conflict | 204 | 1 | A → V in AAM12653. Ref.2 | |||||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||||||
| Helix | 81 – 87 | 7 | ||||||||||||||||||||||||||||||
| Helix | 92 – 95 | 4 | ||||||||||||||||||||||||||||||
| Helix | 98 – 111 | 14 | ||||||||||||||||||||||||||||||
| Helix | 115 – 125 | 11 | ||||||||||||||||||||||||||||||
| Helix | 126 – 128 | 3 | ||||||||||||||||||||||||||||||
| Helix | 133 – 145 | 13 | ||||||||||||||||||||||||||||||
| Turn | 146 – 148 | 3 | ||||||||||||||||||||||||||||||
| Beta strand | 150 – 152 | 3 | ||||||||||||||||||||||||||||||
| Helix | 160 – 168 | 9 | ||||||||||||||||||||||||||||||
| Beta strand | 169 – 171 | 3 | ||||||||||||||||||||||||||||||
| Helix | 178 – 191 | 14 | ||||||||||||||||||||||||||||||
| Helix | 193 – 196 | 4 | ||||||||||||||||||||||||||||||
| Helix | 200 – 203 | 4 | ||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "GAIP, a protein that specifically interacts with the trimeric G protein G alpha i3, is a member of a protein family with a highly conserved core domain." de Vries L., Mousli M., Wurmser A., Farquhar M.G. Proc. Natl. Acad. Sci. U.S.A. 92:11916-11920(1995) [PubMed: 8524874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. |
| [3] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [4] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Subthalamic nucleus. |
| [5] | "The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. Rogers J.Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [6] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [7] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood, Placenta and Testis. |
| [8] | "GAIP is membrane-anchored by palmitoylation and interacts with the activated (GTP-bound) form of G alpha i subunits." de Vries L., Elenko E., Hubler L., Jones T.L.Z., Farquhar M.G. Proc. Natl. Acad. Sci. U.S.A. 93:15203-15208(1996) [PubMed: 8986788] [Abstract] Cited for: PALMITOYLATION. |
| [9] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, MASS SPECTROMETRY. |
| [10] | "Solution structure of human GAIP (Galpha interacting protein): a regulator of G protein signaling." de Alba E., De Vries L., Farquhar M.G., Tjandra N. J. Mol. Biol. 291:927-939(1999) [PubMed: 10452897] [Abstract] Cited for: STRUCTURE BY NMR OF 79-206. |
| [11] | "RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz GTPase-activating protein subfamily." Wang J., Ducret A., Tu Y., Kozasa T., Aebersold R., Ross E.M. J. Biol. Chem. 273:26014-26025(1998) [PubMed: 9748280] [Abstract] Cited for: INHIBITION. |
| [12] | "Erk1/2-dependent phosphorylation of Galpha-interacting protein stimulates its GTPase accelerating activity and autophagy in human colon cancer cells." Ogier-Denis E., Pattingre S., El Benna J., Codogno P. J. Biol. Chem. 275:39090-39095(2000) [PubMed: 10993892] [Abstract] Cited for: PHOSPHORYLATION AT SER-151, MUTAGENESIS OF SER-151. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X91809 mRNA. Translation: CAA62919.1. AF493939 mRNA. Translation: AAM12653.1. AY585188 mRNA. Translation: AAS94232.1. BT009804 mRNA. Translation: AAP88806.1. AK290081 mRNA. Translation: BAF82770.1. AL590548 Genomic DNA. Translation: CAD11902.1. CH471077 Genomic DNA. Translation: EAW75166.1. BC001318 mRNA. Translation: AAH01318.1. BC054337 mRNA. Translation: AAH54337.1. BC063010 mRNA. Translation: AAH63010.1. | |||||||||||||
| IPI | IPI00028108. | ||||||||||||
| RefSeq | NP_001034556.1. NP_005864.1. | ||||||||||||
| UniGene | Hs.422336 | ||||||||||||
3D structure databases | |||||||||||||
| |||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | P49795. 2 interactions. | ||||||||||||
| STRING | P49795. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | P49795. | ||||||||||||
Proteomic databases | |||||||||||||
| PRIDE | P49795. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENST00000332298; ENSP00000333194; ENSG00000171700; Homo sapiens. [Genome view] ENST00000395042; ENSP00000378483; ENSG00000171700; Homo sapiens. [Genome view] | ||||||||||||
| GeneID | 10287. | ||||||||||||
| KEGG | hsa:10287. | ||||||||||||
| UCSC | uc002yhy.1. human. | ||||||||||||
Organism-specific databases | |||||||||||||
| CTD | 10287. | ||||||||||||
| GeneCards | GC20M062174. | ||||||||||||
| H-InvDB | HIX0016021. | ||||||||||||
| HGNC | HGNC:13735. RGS19. | ||||||||||||
| MIM | 605071. gene. | ||||||||||||
| PharmGKB | PA34370. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | P49795. | ||||||||||||
| HOVERGEN | P49795. | ||||||||||||
| OMA | FDKLMHS. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| Pathway_Interaction_DB | trkrpathway. Neurotrophic factor-mediated Trk receptor signaling. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | P49795. | ||||||||||||
| Bgee | P49795. | ||||||||||||
| CleanEx | HS_RGS19. | ||||||||||||
| Genevestigator | P49795. | ||||||||||||
| GermOnline | ENSG00000171700. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR000342. Regulat_G_prot_signal. [Graphical view] | ||||||||||||
| Pfam | PF00615. RGS. 1 hit. [Graphical view] | ||||||||||||
| PRINTS | PR01301. RGSPROTEIN. | ||||||||||||
| ProDom | PD001580. Regl_Gprotein. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||
| SMART | SM00315. RGS. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS50132. RGS. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 38976. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | RGS19_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P49795 Secondary accession number(s): A8K216, Q53XN0, Q8TD60 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 20 Human chromosome 20: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


