P49793 (NUP98_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear pore complex protein Nup98-Nup96

Cleaved into the following 2 chains:

1. Nuclear pore complex protein Nup98
   Alternative name(s):
   98 kDa nucleoporin
   Nucleoporin Nup98
   Short name=Nup98
2. Nuclear pore complex protein Nup96
   Alternative name(s):
   96 kDa nucleoporin
   Nucleoporin Nup96
   Short name=Nup96

Gene names

Name:

Nup98

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1816 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC.
Subunit structure	Part of the nuclear pore complex (NPC). Nup98 interacts directly with Nup96. Nup96 is part of the Nup160 subcomplex in the nuclear pore which is composed of NUP160, NUP133, NUP107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and NUP153 to the nucleus. Nup98 interacts directly with NUP88 and NUP214, subunits of the cytoplasmic filaments of the NPC. Interacts with RAE1. Does not interact with TPR By similarity.
Subcellular location	Nucleus By similarity. Nucleus › nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side By similarity. Note: Nup96 is localized to the nucleoplasmic side of the nuclear pore complex (NPC), at or near the nucleoplasmic basket. Dissociates from the dissasembled NPC structure early during prophase of mitosis By similarity. Colocalized with NUP153 and TPR to the nuclear basket of NPC. Detected in diffuse and discrete intranuclear foci. Ref.4
Domain	Contains G-L-F-G repeats. The FG repeat domains have a direct role in the transport By similarity.
Post-translational modification	The N-terminus is blocked. Isoform 1 is autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96. Ref.3
Sequence similarities	Belongs to the nucleoporin GLFG family. Contains 1 peptidase S59 domain.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport mRNA transport
Cellular component	Membrane Nuclear pore complex Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
PTM	Acetylation Autocatalytic cleavage Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: Compara mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW nuclear pore complex assembly Inferred from sequence or structural similarity. Source: UniProtKB protein import into nucleus, docking Inferred from mutant phenotype Ref.1. Source: RGD
Cellular_component	kinetochore Inferred from electronic annotation. Source: Compara nuclear inclusion body Inferred from direct assay Ref.4. Source: UniProtKB nuclear membrane Inferred from direct assay Ref.4. Source: UniProtKB nuclear periphery Inferred from direct assay Ref.4. Source: UniProtKB nuclear pore nuclear basket Inferred from direct assay Ref.4. Source: UniProtKB nuclear pore outer ring Inferred from sequence or structural similarity. Source: UniProtKB nucleoplasm Inferred from direct assay Ref.1. Source: RGD
Molecular_function	peptide binding Inferred from direct assay Ref.1. Source: RGD structural constituent of nuclear pore Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P49793-1)

Also known as: Nup98-Nup96 precursor;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P49793-2)

The sequence of this isoform differs from the canonical sequence as follows:
     930-938: PQSQSPEVE → TSGREGQRM
     939-1816: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 880	880	Nuclear pore complex protein Nup98		PRO_0000204890
	Chain	881 – 1816	936	Nuclear pore complex protein Nup96		PRO_0000405578
Regions
	Domain	738 – 880	143	Peptidase S59
	Region	1 – 156	156	FG repeats 1
	Region	157 – 213	57	GLEBS; interaction with RAE1 By similarity
	Region	214 – 480	267	FG repeats 2
	Compositional bias	7 – 481	475	Gly/Thr-rich
	Compositional bias	890 – 894	5	Poly-Glu
Amino acid modifications
	Modified residue	524	1	Phosphoserine By similarity
	Modified residue	603	1	N6-acetyllysine By similarity
	Modified residue	608	1	Phosphoserine By similarity
	Modified residue	612	1	Phosphoserine By similarity
	Modified residue	618	1	Phosphoserine By similarity
	Modified residue	623	1	Phosphoserine By similarity
	Modified residue	625	1	Phosphoserine By similarity
	Modified residue	670	1	Phosphothreonine By similarity
	Modified residue	673	1	Phosphoserine By similarity
	Modified residue	681	1	Phosphoserine By similarity
	Modified residue	839	1	Phosphoserine By similarity
	Modified residue	888	1	Phosphoserine By similarity
	Modified residue	934	1	Phosphoserine By similarity
	Modified residue	1027	1	Phosphoserine By similarity
	Modified residue	1042	1	Phosphoserine By similarity
	Modified residue	1059	1	Phosphoserine By similarity
	Modified residue	1069	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	930 – 938	9	PQSQSPEVE → TSGREGQRM in isoform 2.		VSP_040702
	Alternative sequence	939 – 1816	878	Missing in isoform 2.		VSP_040703
Experimental info
	Sequence conflict	11	1	Missing in AAC42054. Ref.1
	Sequence conflict	46	1	N → S in AAC42054. Ref.1
	Sequence conflict	68	1	F → L in AAC42054. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Nup98-Nup96 precursor) [UniParc]. Last modified March 8, 2011. Version 2. Checksum: 70A7D8E23D542B42	FASTA	1,816	197,283
	10 20 30 40 50 60 MFNKSFGTPF GGGTGGFGTT STFGQNTGFG TTSGGAFGTS AFGSSNNTGG LFGNSQTKPG 70 80 90 100 110 120 GLFGTSSFSQ PATSTSTGFG FGTSTGTSNS LFGTANTGTS LFSSQNNAFA QNKPTGFGNF 130 140 150 160 170 180 GTSTSSGGLF GTTNTTSNPF GNTSGSLFGP SSFTAAPTGT TIKFNPPTGT DTMVKAGVST 190 200 210 220 230 240 NISTKHQCIT AMKEYESKSL EELRLEDYQA NRKGPQNQVG AGTTTGLFGS SPATSSATGL 250 260 270 280 290 300 FSSSTTNSAF SYGQNKTAFG TSTTGFGTNP GGLFGQQNQQ TTSLFSKPFG QATTTPNTGF 310 320 330 340 350 360 SFGNTSTLGQ PSTNTMGLFG VTQASQPGGL FGTATNTSTG TAFGTGTGLF GQPNTGFGAV 370 380 390 400 410 420 GSTLFGNNKL TTFGTSTTSA PSFGTTSGGL FGNKPTLTLG TNTNTSNFGF GTNNSGSSIF 430 440 450 460 470 480 GSKPAAGTLG TGLGTGFGTA LGAGQASLFG NNQPKIGGPL GTGAFGAPGF NTSTAILGFG 490 500 510 520 530 540 APQAPVALTD PNASAAQQAV LQQHLNSLTY SPFGDSPLFR NPMSDPKKKE ERLKPTNPAA 550 560 570 580 590 600 QKALTTPTHY KLTPRPATRV RPKALQTTGT AKSHLFDGLD DDEPSLANGA FMPKKSIKKL 610 620 630 640 650 660 VLKNLNNSNL FSPVNHDSED LASPSEYPEN GERFSFLSKP VDENHQQDGD DDSLVSRFYT 670 680 690 700 710 720 NPIAKPIPQT PESAGNKNNS SSNVEDTFIA LNMRAALRNG LEGSSEETSF HDESLQDDRD 730 740 750 760 770 780 EIENSAFQIH PAGIVLTKVG YYTIPSMDDL AKITNEKGEC IVSDFTIGRK GYGSIYFEGD 790 800 810 820 830 840 VNLTNLNLDD IVHIRRKEVI VYVDDNQKPP VGEGLNRKAE VTLDGVWPTD KTSRCLIKSP 850 860 870 880 890 900 DRLADINYEG RLEAVSRKQG AQFKEYRPET GSWVFKVSHF SKYGLQDSDE EEEEHPPKTT 910 920 930 940 950 960 SKKLKTAPLP PAGQATTFQM TLNGKPAPPP QSQSPEVEQL GRVVELDSDM VDITQEPVPD 970 980 990 1000 1010 1020 SVLEESVPED QEPVSASTQI ASSLGINPHV LQIMKASLLV DEEDVDAMEQ RFGHFPSRGD 1030 1040 1050 1060 1070 1080 TAQEICSPRL PISASHSSKS RSIVGGLLQS KFASGTFLSP SASVQECRTP RTSSLMNVPS 1090 1100 1110 1120 1130 1140 TSPWSVPLPL ATVFTVPSPA PEVPLKTVGI RRQPGLVPLE KSITYGKGKL LMDMALFMGR 1150 1160 1170 1180 1190 1200 SFRVGWGPNW TLANSGEQLH GSHELENHQV AESMEYGFLP NPVAVKSLSE SPFKVHLEKL 1210 1220 1230 1240 1250 1260 GLRQRKLDED LQLYQTPLEL KLKHSTVHVD ELCPLIVPNP GVSVIHGYAD WVKKSPRDLL 1270 1280 1290 1300 1310 1320 ELPIVKHWSL TWTLCEALWG HLKELDSQLD EPSEYIQTLE RRRAFSRWLS HTAAPQIEEE 1330 1340 1350 1360 1370 1380 VSLTRRDSPI EAVFSYLTGS RISEACCLAQ QSGDHRLALL LSQLVGSQSV RELLTMQLAD 1390 1400 1410 1420 1430 1440 WHQLQADSFI HDERLRIFAL LAGKPVWQLS EQKQINVCSQ LDWKRTLAIH LWYLLPPTAS 1450 1460 1470 1480 1490 1500 ISRALSMYEE AFQNTCEGDK YACPPLPSYL EGSGCVVEEE KDPQRPLQDV CFHLLKLYSD 1510 1520 1530 1540 1550 1560 RHYGLNQLLE PRSITADPLD YRLSWHLWEV LRALNYTHLS EQCEGVLQAS YAGQLESEGL 1570 1580 1590 1600 1610 1620 WEWAIFVFLH IDNSGMREKA VRELLTRHCQ LSETPESWAK ETFLTQKLCV PAEWIHEAKA 1630 1640 1650 1660 1670 1680 VRAHMESNKH LEALYLFKAG HWNRCHKLVV RHLASDAIIN ENYDYLKGFL EDLAPPERSS 1690 1700 1710 1720 1730 1740 LIQDWETSGL VYLDYIRVIE MLHRIQQVDC SGYELEHLHT KVTSLCNRIE QIPCYNAKDR 1750 1760 1770 1780 1790 1800 LAQSDMAKRV ANLLRVVLSL QHTPDATSNS TPDPQRVPLR LLAPHIGRLP MPEDYALEEL 1810 RGLTQSYLRE LTVGSQ
	Isoform 2 [UniParc]. Checksum: 541463D374531C1F	FASTA	938	97,928
	10 20 30 40 50 60 MFNKSFGTPF GGGTGGFGTT STFGQNTGFG TTSGGAFGTS AFGSSNNTGG LFGNSQTKPG 70 80 90 100 110 120 GLFGTSSFSQ PATSTSTGFG FGTSTGTSNS LFGTANTGTS LFSSQNNAFA QNKPTGFGNF 130 140 150 160 170 180 GTSTSSGGLF GTTNTTSNPF GNTSGSLFGP SSFTAAPTGT TIKFNPPTGT DTMVKAGVST 190 200 210 220 230 240 NISTKHQCIT AMKEYESKSL EELRLEDYQA NRKGPQNQVG AGTTTGLFGS SPATSSATGL 250 260 270 280 290 300 FSSSTTNSAF SYGQNKTAFG TSTTGFGTNP GGLFGQQNQQ TTSLFSKPFG QATTTPNTGF 310 320 330 340 350 360 SFGNTSTLGQ PSTNTMGLFG VTQASQPGGL FGTATNTSTG TAFGTGTGLF GQPNTGFGAV 370 380 390 400 410 420 GSTLFGNNKL TTFGTSTTSA PSFGTTSGGL FGNKPTLTLG TNTNTSNFGF GTNNSGSSIF 430 440 450 460 470 480 GSKPAAGTLG TGLGTGFGTA LGAGQASLFG NNQPKIGGPL GTGAFGAPGF NTSTAILGFG 490 500 510 520 530 540 APQAPVALTD PNASAAQQAV LQQHLNSLTY SPFGDSPLFR NPMSDPKKKE ERLKPTNPAA 550 560 570 580 590 600 QKALTTPTHY KLTPRPATRV RPKALQTTGT AKSHLFDGLD DDEPSLANGA FMPKKSIKKL 610 620 630 640 650 660 VLKNLNNSNL FSPVNHDSED LASPSEYPEN GERFSFLSKP VDENHQQDGD DDSLVSRFYT 670 680 690 700 710 720 NPIAKPIPQT PESAGNKNNS SSNVEDTFIA LNMRAALRNG LEGSSEETSF HDESLQDDRD 730 740 750 760 770 780 EIENSAFQIH PAGIVLTKVG YYTIPSMDDL AKITNEKGEC IVSDFTIGRK GYGSIYFEGD 790 800 810 820 830 840 VNLTNLNLDD IVHIRRKEVI VYVDDNQKPP VGEGLNRKAE VTLDGVWPTD KTSRCLIKSP 850 860 870 880 890 900 DRLADINYEG RLEAVSRKQG AQFKEYRPET GSWVFKVSHF SKYGLQDSDE EEEEHPPKTT 910 920 930 SKKLKTAPLP PAGQATTFQM TLNGKPAPPT SGREGQRM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex." Radu A., Moore M.S., Blobel G. Cell 81:215-222(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[2]	Mural R.J., Li P.W., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S.H., Dew I., Evans C.A., Ferriera S., Flanigan M., Fosler C., Glodek A., Gu Z., Holt R.A., Jennings D., Kraft C.L., Lu F. , Nguyen T., Nusskern D.R., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Wang Z., Woodage T., Zheng X.H., Zhong F. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 1). Strain: Brown Norway.
[3]	"A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96." Fontoura B.M.A., Blobel G., Matunis M.J. J. Cell Biol. 144:1097-1112(1999) [PubMed] [Europe PMC] [Abstract] Cited for: AUTOPROTEOLYTIC CLEAVAGE.
[4]	"Tpr is localized within the nuclear basket of the pore complex and has a role in nuclear protein export." Frosst P., Guan T., Subauste C., Hahn K., Gerace L. J. Cell Biol. 156:617-630(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH473956 Genomic DNA. Translation: EDM18207.1. L39991 mRNA. Translation: AAC42054.1.
IPI	IPI00197380. IPI00389189.
PIR	A56517.
RefSeq	NP_112336.2. NM_031074.2.
UniGene	Rn.11324.
3D structure databases
ProteinModelPortal	P49793.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000027575.
Protein family/group databases
MEROPS	S59.001.
PTM databases
PhosphoSite	P49793.
Proteomic databases
PaxDb	P49793.
PRIDE	P49793.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000027575; ENSRNOP00000027575; ENSRNOG00000020347.
GeneID	81738.
KEGG	rno:81738.
UCSC	RGD:71033. rat.
Organism-specific databases
CTD	4928.
RGD	71033. Nup98.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00550000074799.
HOGENOM	HOG000044579.
HOVERGEN	HBG052702.
KO	K14297.
OrthoDB	EOG437RD1.
Gene expression databases
ArrayExpress	P49793.
Genevestigator	P49793.
GermOnline	ENSRNOG00000020347. Rattus norvegicus.
Family and domain databases
InterPro	IPR021967. Nup96. IPR007230. Peptidase_S59. [Graphical view]
Pfam	PF04096. Nucleoporin2. 1 hit. PF12110. Nup96. 1 hit. [Graphical view]
SUPFAM	SSF82215. Peptidase_S59. 1 hit.
PROSITE	PS51434. NUP_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUP98_RAT

Accession

Primary (citable) accession number: P49793
Secondary accession number(s): D3ZMW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	March 8, 2011
Last modified:	May 1, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program