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P49776 (APH1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
EC=3.6.1.17
Alternative name(s):
Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
Short name=Ap4A hydrolase
Short name=Ap4Aase
Short name=Diadenosine tetraphosphatase
Gene names
Name:aph1
ORF Names:SPCC4G3.02
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length182 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Asymmetrically hydrolyzes Ap4A to yield AMP and ATP.

Catalytic activity

P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP.

Sequence similarities

Contains 1 HIT domain.

Ontologies

Keywords
   LigandGTP-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological processadenosine catabolic process

Inferred from mutant phenotype. Source: GeneDB_Spombe

   Cellular componentcytosol

Inferred from direct assay. Source: GeneDB_Spombe

mitochondrion

Inferred from sequence or structural similarity. Source: GeneDB_Spombe

nucleus

Inferred from direct assay. Source: GeneDB_Spombe

   Molecular functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity

Inferred from mutant phenotype. Source: GeneDB_Spombe

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 182181Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
PRO_0000109788

Regions

Domain3 – 110108HIT
Motif95 – 995Histidine triad motif By similarity

Sites

Active site971Tele-AMP-histidine intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P49776 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: F690D863F438757D

FASTA18220,686
        10         20         30         40         50         60 
MPKQLYFSKF PVGSQVFYRT KLSAAFVNLK PILPGHVLVI PQRAVPRLKD LTPSELTDLF 

        70         80         90        100        110        120 
TSVRKVQQVI EKVFSASASN IGIQDGVDAG QTVPHVHVHI IPRKKADFSE NDLVYSELEK 

       130        140        150        160        170        180 
NEGNLASLYL TGNERYAGDE RPPTSMRQAI PKDEDRKPRT LEEMEKEAQW LKGYFSEEQE 


KE

« Hide

References

« Hide 'large scale' references
[1]"Cloning of the Schizosaccharomyces pombe gene encoding diadenosine 5',5''-P1,P4-tetraphosphate (Ap4A) asymmetrical hydrolase: sequence similarity with the histidine triad (HIT) protein family."
Huang Y., Garrison P.N., Barnes L.D.
Biochem. J. 312:925-932(1995) [PubMed: 8554540] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
Strain: 972 / ATCC 24843.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U32615 Genomic DNA. Translation: AAC49143.1.
CU329672 Genomic DNA. Translation: CAB09779.1.
PIRS64700.
RefSeqNP_587836.1. NM_001022829.1.

3D structure databases

ProteinModelPortalP49776.
ModBaseSearch...

Protein-protein interaction databases

STRINGP49776.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPCC4G3.02.1; SPCC4G3.02.1:pep; SPCC4G3.02.
GeneID2539292.
GenomeReviewsGene locus aph1 in contig CU329672_GR.
KEGGspo:SPCC4G3.02.
NMPDRfig|4896.1.peg.174.

Organism-specific databases

GeneDB_SpombeSPCC4G3.02.

Phylogenomic databases

eggNOGfuNOG09843.
GeneTreeEFGT00050000004936.
HOGENOMHBG396802.
OMAREMERME.
OrthoDBEOG4WDHN5.

Gene expression databases

ArrayExpressP49776.

Family and domain databases

InterProIPR011146. His_triad-like_motif.
IPR011151. His_triad_motif.
IPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
[Graphical view]
Gene3DG3DSA:3.30.428.10. His_triad_motif. 1 hit.
KOK01518.
PANTHERPTHR23089. HIT. 1 hit.
PfamPF01230. HIT. 1 hit.
[Graphical view]
PRINTSPR00332. HISTRIAD.
SUPFAMSSF54197. His_triad-like_motif. 1 hit.
PROSITEPS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPH1_SCHPO
AccessionPrimary (citable) accession number: P49776
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: December 14, 2011
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents