P49776 (APH1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
EC=3.6.1.17

Alternative name(s):
Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
Short name=Ap4A hydrolase
Short name=Ap4Aase
Short name=Diadenosine tetraphosphatase

Gene names

Name:	aph1
ORF Names:	SPCC4G3.02

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4A to yield AMP and ATP.
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP.
Sequence similarities	Contains 1 HIT domain.

Ontologies

Keywords
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	adenosine catabolic process Inferred from mutant phenotype PubMed 10970777. Source: PomBase
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase mitochondrion Inferred from sequence or structural similarity. Source: PomBase nucleus Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from mutant phenotype Ref.1. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 182	181	Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]		PRO_0000109788
Regions
	Domain	3 – 110	108	HIT
	Nucleotide binding	90 – 93	4	Substrate By similarity
	Motif	95 – 99	5	Histidine triad motif
Sites
	Active site	97	1	Tele-AMP-histidine intermediate By similarity
	Binding site	28	1	Substrate By similarity
	Binding site	84	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49776 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: F690D863F438757D	FASTA	182	20,686
	10 20 30 40 50 60 MPKQLYFSKF PVGSQVFYRT KLSAAFVNLK PILPGHVLVI PQRAVPRLKD LTPSELTDLF 70 80 90 100 110 120 TSVRKVQQVI EKVFSASASN IGIQDGVDAG QTVPHVHVHI IPRKKADFSE NDLVYSELEK 130 140 150 160 170 180 NEGNLASLYL TGNERYAGDE RPPTSMRQAI PKDEDRKPRT LEEMEKEAQW LKGYFSEEQE KE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the Schizosaccharomyces pombe gene encoding diadenosine 5',5''-P1,P4-tetraphosphate (Ap4A) asymmetrical hydrolase: sequence similarity with the histidine triad (HIT) protein family." Huang Y., Garrison P.N., Barnes L.D. Biochem. J. 312:925-932(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U32615 Genomic DNA. Translation: AAC49143.1. CU329672 Genomic DNA. Translation: CAB09779.1.
PIR	S64700.
RefSeq	NP_587836.1. NM_001022829.2.
3D structure databases
ProteinModelPortal	P49776.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4690549.
STRING	4896.SPCC4G3.02-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC4G3.02.1; SPCC4G3.02.1:pep; SPCC4G3.02.
GeneID	2539292.
KEGG	spo:SPCC4G3.02.
Organism-specific databases
PomBase	SPCC4G3.02.
Phylogenomic databases
eggNOG	COG0537.
HOGENOM	HOG000164170.
KO	K01518.
OMA	MEEMANE.
OrthoDB	EOG4WDHN5.
Family and domain databases
Gene3D	3.30.428.10. 1 hit.
InterPro	IPR019808. Histidine_triad_CS. IPR001310. Histidine_triad_HIT. IPR011146. HIT-like. [Graphical view]
PANTHER	PTHR23089. PTHR23089. 1 hit.
Pfam	PF01230. HIT. 1 hit. [Graphical view]
PRINTS	PR00332. HISTRIAD.
SUPFAM	SSF54197. His_triad-like_motif. 1 hit.
PROSITE	PS00892. HIT_1. 1 hit. PS51084. HIT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20800459.

Entry information

Entry name

APH1_SCHPO

Accession

Primary (citable) accession number: P49776

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program