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Protein

Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]

Gene

aph1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Asymmetrically hydrolyzes Ap4A to yield AMP and ATP.

Catalytic activityi

P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei28 – 281SubstrateBy similarity
Binding sitei84 – 841SubstrateBy similarity
Active sitei97 – 971Tele-AMP-histidine intermediateBy similarity
Binding sitei99 – 991SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi90 – 934SubstrateBy similarity

GO - Molecular functioni

  • bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Source: PomBase
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • adenosine catabolic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical] (EC:3.6.1.17)
Alternative name(s):
Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
Short name:
Ap4A hydrolase
Short name:
Ap4Aase
Short name:
Diadenosine tetraphosphatase
Gene namesi
Name:aph1
ORF Names:SPCC4G3.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC4G3.02.
PomBaseiSPCC4G3.02. aph1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • mitochondrion Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 182181Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]PRO_0000109788Add
BLAST

Proteomic databases

MaxQBiP49776.

Interactioni

Protein-protein interaction databases

BioGridi275860. 7 interactions.
MINTiMINT-4690549.

Structurei

3D structure databases

ProteinModelPortaliP49776.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 110108HITPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi95 – 995Histidine triad motif

Sequence similaritiesi

Contains 1 HIT domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000164170.
InParanoidiP49776.
KOiK01522.
OMAiYDMLDGE.
OrthoDBiEOG7CK3KJ.
PhylomeDBiP49776.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiIPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
[Graphical view]
PfamiPF01230. HIT. 1 hit.
[Graphical view]
PRINTSiPR00332. HISTRIAD.
SUPFAMiSSF54197. SSF54197. 2 hits.
PROSITEiPS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKQLYFSKF PVGSQVFYRT KLSAAFVNLK PILPGHVLVI PQRAVPRLKD
60 70 80 90 100
LTPSELTDLF TSVRKVQQVI EKVFSASASN IGIQDGVDAG QTVPHVHVHI
110 120 130 140 150
IPRKKADFSE NDLVYSELEK NEGNLASLYL TGNERYAGDE RPPTSMRQAI
160 170 180
PKDEDRKPRT LEEMEKEAQW LKGYFSEEQE KE
Length:182
Mass (Da):20,686
Last modified:January 23, 2007 - v2
Checksum:iF690D863F438757D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32615 Genomic DNA. Translation: AAC49143.1.
CU329672 Genomic DNA. Translation: CAB09779.1.
PIRiS64700.
RefSeqiNP_587836.1. NM_001022829.2.

Genome annotation databases

EnsemblFungiiSPCC4G3.02.1; SPCC4G3.02.1:pep; SPCC4G3.02.
GeneIDi2539292.
KEGGispo:SPCC4G3.02.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32615 Genomic DNA. Translation: AAC49143.1.
CU329672 Genomic DNA. Translation: CAB09779.1.
PIRiS64700.
RefSeqiNP_587836.1. NM_001022829.2.

3D structure databases

ProteinModelPortaliP49776.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275860. 7 interactions.
MINTiMINT-4690549.

Proteomic databases

MaxQBiP49776.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC4G3.02.1; SPCC4G3.02.1:pep; SPCC4G3.02.
GeneIDi2539292.
KEGGispo:SPCC4G3.02.

Organism-specific databases

EuPathDBiFungiDB:SPCC4G3.02.
PomBaseiSPCC4G3.02. aph1.

Phylogenomic databases

HOGENOMiHOG000164170.
InParanoidiP49776.
KOiK01522.
OMAiYDMLDGE.
OrthoDBiEOG7CK3KJ.
PhylomeDBiP49776.

Miscellaneous databases

PROiP49776.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiIPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
[Graphical view]
PfamiPF01230. HIT. 1 hit.
[Graphical view]
PRINTSiPR00332. HISTRIAD.
SUPFAMiSSF54197. SSF54197. 2 hits.
PROSITEiPS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the Schizosaccharomyces pombe gene encoding diadenosine 5',5''-P1,P4-tetraphosphate (Ap4A) asymmetrical hydrolase: sequence similarity with the histidine triad (HIT) protein family."
    Huang Y., Garrison P.N., Barnes L.D.
    Biochem. J. 312:925-932(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiAPH1_SCHPO
AccessioniPrimary (citable) accession number: P49776
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.