Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bis(5'-adenosyl)-triphosphatase

Gene

HNT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves A-5'-PPP-5'A to yield AMP and ADP. Can cleave all dinucleoside polyphosphates, provided the phosphate chain contains at least 3 phosphates and that 1 of the 2 bases composing the nucleotide is a purine. Is most effective on dinucleoside triphosphates. Negatively regulates intracellular dinucleoside polyphosphate levels, which elevate following heat shock.2 Publications

Miscellaneous

Present with 1920 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

P1-P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP.

Cofactori

Mn2+Note: Divalent metal cations. Mn2+ is the preferred ion.

Kineticsi

  1. KM=5.3 µM for P1-P3-bis(5'-adenosyl) triphosphate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei98Tele-AMP-histidine intermediate1 Publication1

    GO - Molecular functioni

    • bis(5'-adenosyl)-triphosphatase activity Source: UniProtKB-EC
    • hydrolase activity Source: SGD
    • nucleoside-triphosphatase activity Source: SGD
    • nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    • nucleoside catabolic process Source: SGD

    Keywordsi

    Molecular functionHydrolase
    LigandNucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29864-MONOMER
    SABIO-RKiP49775

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bis(5'-adenosyl)-triphosphatase (EC:3.6.1.29)
    Alternative name(s):
    AP3A hydrolase
    Short name:
    AP3Aase
    Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
    Dinucleosidetriphosphatase
    Hit family protein 2
    Gene namesi
    Name:HNT2
    Synonyms:APH1
    Ordered Locus Names:YDR305C
    ORF Names:D9740.15
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome IV

    Organism-specific databases

    EuPathDBiFungiDB:YDR305C
    SGDiS000002713 HNT2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi98H → A or D: Fails to reduce intracellular dinucleoside polyphosphate levels. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001098011 – 206Bis(5'-adenosyl)-triphosphataseAdd BLAST206

    Proteomic databases

    MaxQBiP49775
    PaxDbiP49775
    PRIDEiP49775

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi32357, 80 interactors
    DIPiDIP-4620N
    IntActiP49775, 2 interactors
    MINTiP49775
    STRINGi4932.YDR305C

    Structurei

    3D structure databases

    ProteinModelPortaliP49775
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini3 – 115HITPROSITE-ProRule annotationAdd BLAST113

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi96 – 100Histidine triad motif5

    Phylogenomic databases

    HOGENOMiHOG000164170
    KOiK01522
    OrthoDBiEOG092C1QR6

    Family and domain databases

    InterProiView protein in InterPro
    IPR019808 Histidine_triad_CS
    IPR001310 Histidine_triad_HIT
    IPR011146 HIT-like
    IPR036265 HIT-like_sf
    PANTHERiPTHR23089 PTHR23089, 1 hit
    PfamiView protein in Pfam
    PF01230 HIT, 1 hit
    SUPFAMiSSF54197 SSF54197, 2 hits
    PROSITEiView protein in PROSITE
    PS00892 HIT_1, 1 hit
    PS51084 HIT_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P49775-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNKPIYFSKF LVTEQVFYKS KYTYALVNLK PIVPGHVLIV PLRTTVLNLS
    60 70 80 90 100
    DLTMPESQDY FKTLQLIHRF IKWQYKADSI NVAIQDGPEA GQSVPHLHTH
    110 120 130 140 150
    IIPRYKINNV GDLIYDKLDH WDGNGTLTDW QGRRDEYLGV GGRQARKNNS
    160 170 180 190 200
    TSATVDGDEL SQGPNVLKPD SQRKVRALTE MKKEAEDLQA RLEEFVSSDP

    GLTQWL
    Length:206
    Mass (Da):23,542
    Last modified:October 2, 2007 - v2
    Checksum:i4143F7371805B378
    GO

    Sequence cautioni

    The sequence AAB64741 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence DAA12144 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U28374 Genomic DNA Translation: AAB64741.1 Different initiation.
    AY389296 mRNA Translation: AAQ97228.1
    AY389295 mRNA Translation: AAQ97227.1
    BK006938 Genomic DNA Translation: DAA12144.1 Different initiation.
    PIRiS61191
    RefSeqiNP_010591.1, NM_001180613.1

    Genome annotation databases

    GeneIDi851899
    KEGGisce:YDR305C

    Similar proteinsi

    Entry informationi

    Entry nameiHNT2_YEAST
    AccessioniPrimary (citable) accession number: P49775
    Secondary accession number(s): D6VST4, Q6TQU2, Q6TQU3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 2, 2007
    Last modified: March 28, 2018
    This is version 132 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health