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Protein

Bis(5'-adenosyl)-triphosphatase

Gene

HNT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves A-5'-PPP-5'A to yield AMP and ADP. Can cleave all dinucleoside polyphosphates, provided the phosphate chain contains at least 3 phosphates and that 1 of the 2 bases composing the nucleotide is a purine. Is most effective on dinucleoside triphosphates. Negatively regulates intracellular dinucleoside polyphosphate levels, which elevate following heat shock.2 Publications

Catalytic activityi

P(1)-P(3)-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP.

Cofactori

Mn2+Note: Divalent metal cations. Mn2+ is the preferred ion.

Kineticsi

  1. KM=5.3 µM for P(1)-P(3)-bis(5'-adenosyl) triphosphate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei98Tele-AMP-histidine intermediate1 Publication1

    GO - Molecular functioni

    • bis(5'-adenosyl)-triphosphatase activity Source: UniProtKB-EC
    • hydrolase activity Source: SGD
    • nucleoside-triphosphatase activity Source: SGD
    • nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    • nucleoside catabolic process Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29864-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bis(5'-adenosyl)-triphosphatase (EC:3.6.1.29)
    Alternative name(s):
    AP3A hydrolase
    Short name:
    AP3Aase
    Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
    Dinucleosidetriphosphatase
    Hit family protein 2
    Gene namesi
    Name:HNT2
    Synonyms:APH1
    Ordered Locus Names:YDR305C
    ORF Names:D9740.15
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome IV

    Organism-specific databases

    EuPathDBiFungiDB:YDR305C.
    SGDiS000002713. HNT2.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi98H → A or D: Fails to reduce intracellular dinucleoside polyphosphate levels. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001098011 – 206Bis(5'-adenosyl)-triphosphataseAdd BLAST206

    Proteomic databases

    MaxQBiP49775.
    PRIDEiP49775.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi32357. 31 interactors.
    DIPiDIP-4620N.
    IntActiP49775. 1 interactor.
    MINTiMINT-473047.

    Structurei

    3D structure databases

    ProteinModelPortaliP49775.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini3 – 115HITPROSITE-ProRule annotationAdd BLAST113

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi96 – 100Histidine triad motif5

    Sequence similaritiesi

    Contains 1 HIT domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOGENOMiHOG000164170.
    KOiK01522.
    OrthoDBiEOG092C1QR6.

    Family and domain databases

    Gene3Di3.30.428.10. 2 hits.
    InterProiIPR019808. Histidine_triad_CS.
    IPR001310. Histidine_triad_HIT.
    IPR011146. HIT-like.
    [Graphical view]
    PANTHERiPTHR23089. PTHR23089. 2 hits.
    PfamiPF01230. HIT. 1 hit.
    [Graphical view]
    SUPFAMiSSF54197. SSF54197. 2 hits.
    PROSITEiPS00892. HIT_1. 1 hit.
    PS51084. HIT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P49775-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNKPIYFSKF LVTEQVFYKS KYTYALVNLK PIVPGHVLIV PLRTTVLNLS
    60 70 80 90 100
    DLTMPESQDY FKTLQLIHRF IKWQYKADSI NVAIQDGPEA GQSVPHLHTH
    110 120 130 140 150
    IIPRYKINNV GDLIYDKLDH WDGNGTLTDW QGRRDEYLGV GGRQARKNNS
    160 170 180 190 200
    TSATVDGDEL SQGPNVLKPD SQRKVRALTE MKKEAEDLQA RLEEFVSSDP

    GLTQWL
    Length:206
    Mass (Da):23,542
    Last modified:October 2, 2007 - v2
    Checksum:i4143F7371805B378
    GO

    Sequence cautioni

    The sequence AAB64741 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence DAA12144 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U28374 Genomic DNA. Translation: AAB64741.1. Different initiation.
    AY389296 mRNA. Translation: AAQ97228.1.
    AY389295 mRNA. Translation: AAQ97227.1.
    BK006938 Genomic DNA. Translation: DAA12144.1. Different initiation.
    PIRiS61191.
    RefSeqiNP_010591.1. NM_001180613.1.

    Genome annotation databases

    GeneIDi851899.
    KEGGisce:YDR305C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U28374 Genomic DNA. Translation: AAB64741.1. Different initiation.
    AY389296 mRNA. Translation: AAQ97228.1.
    AY389295 mRNA. Translation: AAQ97227.1.
    BK006938 Genomic DNA. Translation: DAA12144.1. Different initiation.
    PIRiS61191.
    RefSeqiNP_010591.1. NM_001180613.1.

    3D structure databases

    ProteinModelPortaliP49775.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi32357. 31 interactors.
    DIPiDIP-4620N.
    IntActiP49775. 1 interactor.
    MINTiMINT-473047.

    Proteomic databases

    MaxQBiP49775.
    PRIDEiP49775.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi851899.
    KEGGisce:YDR305C.

    Organism-specific databases

    EuPathDBiFungiDB:YDR305C.
    SGDiS000002713. HNT2.

    Phylogenomic databases

    HOGENOMiHOG000164170.
    KOiK01522.
    OrthoDBiEOG092C1QR6.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29864-MONOMER.

    Miscellaneous databases

    PROiP49775.

    Family and domain databases

    Gene3Di3.30.428.10. 2 hits.
    InterProiIPR019808. Histidine_triad_CS.
    IPR001310. Histidine_triad_HIT.
    IPR011146. HIT-like.
    [Graphical view]
    PANTHERiPTHR23089. PTHR23089. 2 hits.
    PfamiPF01230. HIT. 1 hit.
    [Graphical view]
    SUPFAMiSSF54197. SSF54197. 2 hits.
    PROSITEiPS00892. HIT_1. 1 hit.
    PS51084. HIT_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHNT2_YEAST
    AccessioniPrimary (citable) accession number: P49775
    Secondary accession number(s): D6VST4, Q6TQU2, Q6TQU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 2, 2007
    Last modified: November 2, 2016
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1920 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.