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Protein

Fms-related tyrosine kinase 3 ligand

Gene

Flt3lg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

GO - Molecular functioni

  1. protein homodimerization activity Source: MGI
  2. receptor tyrosine kinase binding Source: MGI

GO - Biological processi

  1. embryonic hemopoiesis Source: MGI
  2. lymphocyte differentiation Source: MGI
  3. positive regulation of cell proliferation Source: MGI
  4. positive regulation of myoblast differentiation Source: MGI
  5. positive regulation of myoblast fusion Source: MGI
  6. positive regulation of natural killer cell differentiation Source: MGI
  7. positive regulation of protein phosphorylation Source: MGI
  8. regulation of myeloid dendritic cell activation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Names & Taxonomyi

Protein namesi
Recommended name:
Fms-related tyrosine kinase 3 ligand
Short name:
Flt3 ligand
Short name:
Flt3L
Alternative name(s):
SL cytokine
Gene namesi
Name:Flt3lg
Synonyms:Flt3l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:95560. Flt3l.

Subcellular locationi

Isoform 3 : Secreted
Note: Biologically active.
Isoform 4 : Secreted
Note: Inactive.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 189163ExtracellularSequence AnalysisAdd
BLAST
Transmembranei190 – 21021HelicalSequence AnalysisAdd
BLAST
Topological domaini211 – 23222CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. extracellular space Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
  4. intrinsic component of external side of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 232206Fms-related tyrosine kinase 3 ligandPRO_0000021282Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 112By similarity
Disulfide bondi71 ↔ 156By similarity
Disulfide bondi120 ↔ 161By similarity
Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi152 – 1521N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP49772.
PRIDEiP49772.

Expressioni

Gene expression databases

BgeeiP49772.
CleanExiMM_FLT3L.
ExpressionAtlasiP49772. baseline and differential.
GenevestigatoriP49772.

Interactioni

Subunit structurei

Homodimer (soluble isoform).By similarity

Structurei

3D structure databases

ProteinModelPortaliP49772.
SMRiP49772. Positions 30-162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0102.
GeneTreeiENSGT00530000064424.
HOGENOMiHOG000029239.
HOVERGENiHBG051630.
InParanoidiP49772.
KOiK05454.
OMAiCLEGLIM.
OrthoDBiEOG7HTHJG.
PhylomeDBiP49772.
TreeFamiTF338225.

Family and domain databases

Gene3Di1.20.1250.10. 2 hits.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR004213. Flt3_lig.
[Graphical view]
PANTHERiPTHR11032. PTHR11032. 1 hit.
PfamiPF02947. Flt3_lig. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P49772-1) [UniParc]FASTAAdd to basket

Also known as: 6C

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVLAPAWSP NSSLLLLLLL LSPCLRGTPD CYFSHSPISS NFKVKFRELT
60 70 80 90 100
DHLLKDYPVT VAVNLQDEKH CKALWSLFLA QRWIEQLKTV AGSKMQTLLE
110 120 130 140 150
DVNTEIHFVT SCTFQPLPEC LRFVQTNISH LLKDTCTQLL ALKPCIGKAC
160 170 180 190 200
QNFSRCLEVQ CQPDSSTLLP PRSPIALEAT ELPEPRPRQL LLLLLLLLPL
210 220 230
TLVLLAAAWG LRWQRARRRG ELHPGVPLPS HP
Length:232
Mass (Da):26,141
Last modified:September 30, 1996 - v1
Checksum:i3A3680D3CB69FBA6
GO
Isoform 2 (identifier: P49772-2) [UniParc]FASTAAdd to basket

Also known as: 5H

The sequence of this isoform differs from the canonical sequence as follows:
     164-232: DSSTLLPPRS...HPGVPLPSHP → GNGGPRAQHH...SSFLKMYLYV

Show »
Length:220
Mass (Da):24,674
Checksum:i8D2FB35D2A706E53
GO
Isoform 3 (identifier: P49772-3) [UniParc]FASTAAdd to basket

Also known as: E6

The sequence of this isoform differs from the canonical sequence as follows:
     164-169: DSSTLL → GSHYAG
     170-232: Missing.

Show »
Length:169
Mass (Da):19,037
Checksum:iA6DDE0C0A5F23623
GO
Isoform 4 (identifier: P49772-4) [UniParc]FASTAAdd to basket

Also known as: E6Delta16

The sequence of this isoform differs from the canonical sequence as follows:
     159-163: VQCQP → GLIMQARLTLNSKQSSCLSVLSAGIT

Show »
Length:253
Mass (Da):28,259
Checksum:i92164080DC647D99
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti141 – 1411A → G in AAA18000 (PubMed:8145851).Curated
Sequence conflicti198 – 1981Missing (PubMed:7505204).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei159 – 1635VQCQP → GLIMQARLTLNSKQSSCLSV LSAGIT in isoform 4. CuratedVSP_004256
Alternative sequencei164 – 23269DSSTL…LPSHP → GNGGPRAQHHGATRLTATAL LTVCPGLLLPLVGTSHMFFL PYFLSFLSSFLKMYLYV in isoform 2. CuratedVSP_004253Add
BLAST
Alternative sequencei164 – 1696DSSTLL → GSHYAG in isoform 3. CuratedVSP_004254
Alternative sequencei170 – 23263Missing in isoform 3. CuratedVSP_004255Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04807 mRNA. Translation: AAA18000.1.
L23636 mRNA. Translation: AAA39436.1.
U29875 Genomic DNA. Translation: AAA90951.1.
U29875 Genomic DNA. Translation: AAA90952.1.
S76459 mRNA. Translation: AAB33069.1.
S76461 mRNA. Translation: AAB33070.1.
S76464 mRNA. Translation: AAB33071.1.
U44024 Genomic DNA. Translation: AAA93307.1.
U44024 Genomic DNA. Translation: AAA93306.1.
AK020105 mRNA. Translation: BAB31997.1.
CCDSiCCDS39948.1. [P49772-1]
PIRiA49265.
I58343.
RefSeqiNP_038548.3. NM_013520.3. [P49772-1]
UniGeneiMm.1658.

Genome annotation databases

EnsembliENSMUST00000085377; ENSMUSP00000082492; ENSMUSG00000089989. [P49772-1]
ENSMUST00000123381; ENSMUSP00000115222; ENSMUSG00000089989. [P49772-1]
ENSMUST00000125500; ENSMUSP00000119175; ENSMUSG00000089989. [P49772-4]
ENSMUST00000130628; ENSMUSP00000118193; ENSMUSG00000089989. [P49772-2]
ENSMUST00000146760; ENSMUSP00000123506; ENSMUSG00000089989. [P49772-1]
GeneIDi14256.
KEGGimmu:14256.
UCSCiuc009gtn.2. mouse. [P49772-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04807 mRNA. Translation: AAA18000.1.
L23636 mRNA. Translation: AAA39436.1.
U29875 Genomic DNA. Translation: AAA90951.1.
U29875 Genomic DNA. Translation: AAA90952.1.
S76459 mRNA. Translation: AAB33069.1.
S76461 mRNA. Translation: AAB33070.1.
S76464 mRNA. Translation: AAB33071.1.
U44024 Genomic DNA. Translation: AAA93307.1.
U44024 Genomic DNA. Translation: AAA93306.1.
AK020105 mRNA. Translation: BAB31997.1.
CCDSiCCDS39948.1. [P49772-1]
PIRiA49265.
I58343.
RefSeqiNP_038548.3. NM_013520.3. [P49772-1]
UniGeneiMm.1658.

3D structure databases

ProteinModelPortaliP49772.
SMRiP49772. Positions 30-162.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP49772.
PRIDEiP49772.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000085377; ENSMUSP00000082492; ENSMUSG00000089989. [P49772-1]
ENSMUST00000123381; ENSMUSP00000115222; ENSMUSG00000089989. [P49772-1]
ENSMUST00000125500; ENSMUSP00000119175; ENSMUSG00000089989. [P49772-4]
ENSMUST00000130628; ENSMUSP00000118193; ENSMUSG00000089989. [P49772-2]
ENSMUST00000146760; ENSMUSP00000123506; ENSMUSG00000089989. [P49772-1]
GeneIDi14256.
KEGGimmu:14256.
UCSCiuc009gtn.2. mouse. [P49772-1]

Organism-specific databases

CTDi14256.
MGIiMGI:95560. Flt3l.

Phylogenomic databases

eggNOGiCOG0102.
GeneTreeiENSGT00530000064424.
HOGENOMiHOG000029239.
HOVERGENiHBG051630.
InParanoidiP49772.
KOiK05454.
OMAiCLEGLIM.
OrthoDBiEOG7HTHJG.
PhylomeDBiP49772.
TreeFamiTF338225.

Miscellaneous databases

NextBioi285587.
PROiP49772.
SOURCEiSearch...

Gene expression databases

BgeeiP49772.
CleanExiMM_FLT3L.
ExpressionAtlasiP49772. baseline and differential.
GenevestigatoriP49772.

Family and domain databases

Gene3Di1.20.1250.10. 2 hits.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR004213. Flt3_lig.
[Graphical view]
PANTHERiPTHR11032. PTHR11032. 1 hit.
PfamiPF02947. Flt3_lig. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning of a ligand for the flt3/flk-2 tyrosine kinase receptor: a proliferative factor for primitive hematopoietic cells."
    Lyman S.D., James L., Vanden Bos T., Devries P., Brasel K., Gliniak B., Hollingsworth L.T., Picha K.S., McKenna H.J., Splett R.R., Fletcher F.A., Maraskovsky E., Farrah T., Foxworthe D., Williams D.E., Beckmann M.P.
    Cell 75:1157-1167(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: SJL/J.
  3. "Structural analysis of human and murine flt3 ligand genomic loci."
    Lyman S.D., Stocking K., Davison B., Fletcher F., Johnson L., Escobar S.
    Oncogene 11:1165-1172(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
  4. "Identification of soluble and membrane-bound isoforms of the murine flt3 ligand generated by alternative splicing of mRNAs."
    Lyman S.D., James L., Escobar S., Downey H., de Vries P., Brasel K., Stocking K., Beckmann M.P., Copeland N.G., Cleveland L.S.
    Oncogene 10:149-157(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryo.

Entry informationi

Entry nameiFLT3L_MOUSE
AccessioniPrimary (citable) accession number: P49772
Secondary accession number(s): Q64085
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1996
Last sequence update: September 30, 1996
Last modified: February 3, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.