P49772 (FLT3L_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fms-related tyrosine kinase 3 ligand
Short name=Flt3 ligand
Short name=Flt3L

Alternative name(s):
SL cytokine

Gene names

Name:	Flt3lg
Synonyms:	Flt3l

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Subunit structure	Homodimer (soluble isoform) By similarity.
Subcellular location	Cell membrane; Single-pass type I membrane protein. Isoform 3: Secreted. Note: Biologically active. Isoform 4: Secreted. Note: Inactive.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	embryonic hemopoiesis Inferred from electronic annotation. Source: Compara lymphocyte differentiation Inferred from mutant phenotype PubMed 12387740. Source: MGI positive regulation of cell proliferation Inferred from direct assay Ref.2. Source: MGI positive regulation of natural killer cell differentiation Inferred from mutant phenotype PubMed 14607906. Source: MGI
Cellular_component	extracellular space Inferred from direct assay Ref.2PubMed 8896402. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to external side of plasma membrane Inferred from direct assay Ref.2PubMed 8896402. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	receptor tyrosine kinase binding Inferred from direct assay PubMed 7531700. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P49772-1)

Also known as: 6C;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P49772-2)

Also known as: 5H;

The sequence of this isoform differs from the canonical sequence as follows:
     164-232: DSSTLLPPRS...HPGVPLPSHP → GNGGPRAQHH...SSFLKMYLYV

Isoform 3 (identifier: P49772-3)

Also known as: E6;

The sequence of this isoform differs from the canonical sequence as follows:
     164-169: DSSTLL → GSHYAG
     170-232: Missing.

Isoform 4 (identifier: P49772-4)

Also known as: E6Delta16;

The sequence of this isoform differs from the canonical sequence as follows:
     159-163: VQCQP → GLIMQARLTLNSKQSSCLSVLSAGIT

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 232	206	Fms-related tyrosine kinase 3 ligand		PRO_0000021282
Regions
	Topological domain	27 – 189	163	Extracellular Potential
	Transmembrane	190 – 210	21	Helical; Potential
	Topological domain	211 – 232	22	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	152	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 112		By similarity
	Disulfide bond	71 ↔ 156		By similarity
	Disulfide bond	120 ↔ 161		By similarity
Natural variations
	Alternative sequence	159 – 163	5	VQCQP → GLIMQARLTLNSKQSSCLSV LSAGIT in isoform 4.		VSP_004256
	Alternative sequence	164 – 232	69	DSSTL…LPSHP → GNGGPRAQHHGATRLTATAL LTVCPGLLLPLVGTSHMFFL PYFLSFLSSFLKMYLYV in isoform 2.		VSP_004253
	Alternative sequence	164 – 169	6	DSSTLL → GSHYAG in isoform 3.		VSP_004254
	Alternative sequence	170 – 232	63	Missing in isoform 3.		VSP_004255
Experimental info
	Sequence conflict	141	1	A → G in AAA18000. Ref.1
	Sequence conflict	198	1	Missing Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (6C) [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3A3680D3CB69FBA6	FASTA	232	26,141
	10 20 30 40 50 60 MTVLAPAWSP NSSLLLLLLL LSPCLRGTPD CYFSHSPISS NFKVKFRELT DHLLKDYPVT 70 80 90 100 110 120 VAVNLQDEKH CKALWSLFLA QRWIEQLKTV AGSKMQTLLE DVNTEIHFVT SCTFQPLPEC 130 140 150 160 170 180 LRFVQTNISH LLKDTCTQLL ALKPCIGKAC QNFSRCLEVQ CQPDSSTLLP PRSPIALEAT 190 200 210 220 230 ELPEPRPRQL LLLLLLLLPL TLVLLAAAWG LRWQRARRRG ELHPGVPLPS HP
	Isoform 2 (5H) [UniParc]. Checksum: 8D2FB35D2A706E53	FASTA	220	24,674
	10 20 30 40 50 60 MTVLAPAWSP NSSLLLLLLL LSPCLRGTPD CYFSHSPISS NFKVKFRELT DHLLKDYPVT 70 80 90 100 110 120 VAVNLQDEKH CKALWSLFLA QRWIEQLKTV AGSKMQTLLE DVNTEIHFVT SCTFQPLPEC 130 140 150 160 170 180 LRFVQTNISH LLKDTCTQLL ALKPCIGKAC QNFSRCLEVQ CQPGNGGPRA QHHGATRLTA 190 200 210 220 TALLTVCPGL LLPLVGTSHM FFLPYFLSFL SSFLKMYLYV
	Isoform 3 (E6) [UniParc]. Checksum: A6DDE0C0A5F23623	FASTA	169	19,037
	10 20 30 40 50 60 MTVLAPAWSP NSSLLLLLLL LSPCLRGTPD CYFSHSPISS NFKVKFRELT DHLLKDYPVT 70 80 90 100 110 120 VAVNLQDEKH CKALWSLFLA QRWIEQLKTV AGSKMQTLLE DVNTEIHFVT SCTFQPLPEC 130 140 150 160 LRFVQTNISH LLKDTCTQLL ALKPCIGKAC QNFSRCLEVQ CQPGSHYAG
	Isoform 4 (E6Delta16) [UniParc]. Checksum: 92164080DC647D99	FASTA	253	28,259
	10 20 30 40 50 60 MTVLAPAWSP NSSLLLLLLL LSPCLRGTPD CYFSHSPISS NFKVKFRELT DHLLKDYPVT 70 80 90 100 110 120 VAVNLQDEKH CKALWSLFLA QRWIEQLKTV AGSKMQTLLE DVNTEIHFVT SCTFQPLPEC 130 140 150 160 170 180 LRFVQTNISH LLKDTCTQLL ALKPCIGKAC QNFSRCLEGL IMQARLTLNS KQSSCLSVLS 190 200 210 220 230 240 AGITDSSTLL PPRSPIALEA TELPEPRPRQ LLLLLLLLLP LTLVLLAAAW GLRWQRARRR 250 GELHPGVPLP SHP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs." Hannum C., Culpepper J., Campbell D., McClanahan T., Zurawski S., Bazan J.F., Kastelein R., Hudak S., Wagner J., Mattson J., Luh J., Duda G., Martina N., Peterson D., Menon S., Shanafelt A., Muench M., Kelner G. , Namikawa R., Rennick D., Roncarolo M.G., Zlotnik A., Rosnet O., Dubreuil P., Birnbaum D., Lee F. Nature 368:643-648(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of a ligand for the flt3/flk-2 tyrosine kinase receptor: a proliferative factor for primitive hematopoietic cells." Lyman S.D., James L., Vanden Bos T., Devries P., Brasel K., Gliniak B., Hollingsworth L.T., Picha K.S., McKenna H.J., Splett R.R., Fletcher F.A., Maraskovsky E., Farrah T., Foxworthe D., Williams D.E., Beckmann M.P. Cell 75:1157-1167(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: SJL/J.
[3]	"Structural analysis of human and murine flt3 ligand genomic loci." Lyman S.D., Stocking K., Davison B., Fletcher F., Johnson L., Escobar S. Oncogene 11:1165-1172(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
[4]	"Identification of soluble and membrane-bound isoforms of the murine flt3 ligand generated by alternative splicing of mRNAs." Lyman S.D., James L., Escobar S., Downey H., de Vries P., Brasel K., Stocking K., Beckmann M.P., Copeland N.G., Cleveland L.S. Oncogene 10:149-157(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[5]	McClanahan T., Culpepper J., Campbell D., Wagner J., Franz-Bacon K., Mattson J., Tsai S., Luh J., Guimares M.J., Mattei M.-G., Rosnet O., Birnbaum D., Hannum C. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U04807 mRNA. Translation: AAA18000.1. L23636 mRNA. Translation: AAA39436.1. U29875 Genomic DNA. Translation: AAA90951.1. U29875 Genomic DNA. Translation: AAA90952.1. S76459 mRNA. Translation: AAB33069.1. S76461 mRNA. Translation: AAB33070.1. S76464 mRNA. Translation: AAB33071.1. U44024 Genomic DNA. Translation: AAA93307.1. U44024 Genomic DNA. Translation: AAA93306.1. AK020105 mRNA. Translation: BAB31997.1.
IPI	IPI00223433. IPI00223434. IPI00223435. IPI00468562.
PIR	A49265. I58343.
RefSeq	NP_038548.3. NM_013520.3.
UniGene	Mm.1658.
3D structure databases
ProteinModelPortal	P49772.
SMR	P49772. Positions 30-162.
ModBase	Search...
Proteomic databases
PaxDb	P49772.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000085377; ENSMUSP00000082492; ENSMUSG00000089989. ENSMUST00000123381; ENSMUSP00000115222; ENSMUSG00000089989. ENSMUST00000125500; ENSMUSP00000119175; ENSMUSG00000089989. ENSMUST00000130628; ENSMUSP00000118193; ENSMUSG00000089989. ENSMUST00000146760; ENSMUSP00000123506; ENSMUSG00000089989.
GeneID	14256.
KEGG	mmu:14256.
Organism-specific databases
CTD	14256.
MGI	MGI:95560. Flt3l.
Phylogenomic databases
eggNOG	COG0102.
GeneTree	ENSGT00530000064424.
HOGENOM	HOG000029239.
HOVERGEN	HBG051630.
KO	K05454.
OMA	AVNTEIH.
Gene expression databases
ArrayExpress	P49772.
Bgee	P49772.
CleanEx	MM_FLT3L.
Genevestigator	P49772.
GermOnline	ENSMUSG00000074129. Mus musculus.
Family and domain databases
Gene3D	1.20.1250.10. 2 hits.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR004213. Flt3_lig. [Graphical view]
PANTHER	PTHR11032. PTHR11032. 1 hit.
Pfam	PF02947. Flt3_lig. 1 hit. [Graphical view]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
ProtoNet	Search...
Other
NextBio	285587.
SOURCE	Search...

Entry information

Entry name

FLT3L_MOUSE

Accession

Primary (citable) accession number: P49772
Secondary accession number(s): Q64085

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program