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P49772

- FLT3L_MOUSE

UniProt

P49772 - FLT3L_MOUSE

Protein

Fms-related tyrosine kinase 3 ligand

Gene

Flt3lg

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

    GO - Molecular functioni

    1. protein homodimerization activity Source: MGI
    2. receptor tyrosine kinase binding Source: MGI

    GO - Biological processi

    1. embryonic hemopoiesis Source: Ensembl
    2. lymphocyte differentiation Source: MGI
    3. positive regulation of cell proliferation Source: MGI
    4. positive regulation of natural killer cell differentiation Source: MGI
    5. positive regulation of protein phosphorylation Source: MGI
    6. regulation of myeloid dendritic cell activation Source: MGI

    Keywords - Molecular functioni

    Cytokine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fms-related tyrosine kinase 3 ligand
    Short name:
    Flt3 ligand
    Short name:
    Flt3L
    Alternative name(s):
    SL cytokine
    Gene namesi
    Name:Flt3lg
    Synonyms:Flt3l
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:95560. Flt3l.

    Subcellular locationi

    Isoform 3 : Secreted
    Note: Biologically active.
    Isoform 4 : Secreted
    Note: Inactive.

    GO - Cellular componenti

    1. cell surface Source: MGI
    2. extracellular space Source: MGI
    3. integral component of membrane Source: UniProtKB-KW
    4. intrinsic component of external side of plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 232206Fms-related tyrosine kinase 3 ligandPRO_0000021282Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 112By similarity
    Disulfide bondi71 ↔ 156By similarity
    Disulfide bondi120 ↔ 161By similarity
    Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi152 – 1521N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP49772.
    PRIDEiP49772.

    Expressioni

    Gene expression databases

    BgeeiP49772.
    CleanExiMM_FLT3L.
    GenevestigatoriP49772.

    Interactioni

    Subunit structurei

    Homodimer (soluble isoform).By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP49772.
    SMRiP49772. Positions 30-162.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini27 – 189163ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini211 – 23222CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei190 – 21021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0102.
    GeneTreeiENSGT00530000064424.
    HOGENOMiHOG000029239.
    HOVERGENiHBG051630.
    KOiK05454.
    OMAiAVNTEIH.
    OrthoDBiEOG7HTHJG.
    PhylomeDBiP49772.
    TreeFamiTF338225.

    Family and domain databases

    Gene3Di1.20.1250.10. 2 hits.
    InterProiIPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR004213. Flt3_lig.
    [Graphical view]
    PANTHERiPTHR11032. PTHR11032. 1 hit.
    PfamiPF02947. Flt3_lig. 1 hit.
    [Graphical view]
    SUPFAMiSSF47266. SSF47266. 1 hit.

    Sequences (4)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P49772-1) [UniParc]FASTAAdd to Basket

    Also known as: 6C

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTVLAPAWSP NSSLLLLLLL LSPCLRGTPD CYFSHSPISS NFKVKFRELT    50
    DHLLKDYPVT VAVNLQDEKH CKALWSLFLA QRWIEQLKTV AGSKMQTLLE 100
    DVNTEIHFVT SCTFQPLPEC LRFVQTNISH LLKDTCTQLL ALKPCIGKAC 150
    QNFSRCLEVQ CQPDSSTLLP PRSPIALEAT ELPEPRPRQL LLLLLLLLPL 200
    TLVLLAAAWG LRWQRARRRG ELHPGVPLPS HP 232
    Length:232
    Mass (Da):26,141
    Last modified:October 1, 1996 - v1
    Checksum:i3A3680D3CB69FBA6
    GO
    Isoform 2 (identifier: P49772-2) [UniParc]FASTAAdd to Basket

    Also known as: 5H

    The sequence of this isoform differs from the canonical sequence as follows:
         164-232: DSSTLLPPRS...HPGVPLPSHP → GNGGPRAQHH...SSFLKMYLYV

    Show »
    Length:220
    Mass (Da):24,674
    Checksum:i8D2FB35D2A706E53
    GO
    Isoform 3 (identifier: P49772-3) [UniParc]FASTAAdd to Basket

    Also known as: E6

    The sequence of this isoform differs from the canonical sequence as follows:
         164-169: DSSTLL → GSHYAG
         170-232: Missing.

    Show »
    Length:169
    Mass (Da):19,037
    Checksum:iA6DDE0C0A5F23623
    GO
    Isoform 4 (identifier: P49772-4) [UniParc]FASTAAdd to Basket

    Also known as: E6Delta16

    The sequence of this isoform differs from the canonical sequence as follows:
         159-163: VQCQP → GLIMQARLTLNSKQSSCLSVLSAGIT

    Show »
    Length:253
    Mass (Da):28,259
    Checksum:i92164080DC647D99
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti141 – 1411A → G in AAA18000. (PubMed:8145851)Curated
    Sequence conflicti198 – 1981Missing(PubMed:7505204)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei159 – 1635VQCQP → GLIMQARLTLNSKQSSCLSV LSAGIT in isoform 4. CuratedVSP_004256
    Alternative sequencei164 – 23269DSSTL…LPSHP → GNGGPRAQHHGATRLTATAL LTVCPGLLLPLVGTSHMFFL PYFLSFLSSFLKMYLYV in isoform 2. CuratedVSP_004253Add
    BLAST
    Alternative sequencei164 – 1696DSSTLL → GSHYAG in isoform 3. CuratedVSP_004254
    Alternative sequencei170 – 23263Missing in isoform 3. CuratedVSP_004255Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04807 mRNA. Translation: AAA18000.1.
    L23636 mRNA. Translation: AAA39436.1.
    U29875 Genomic DNA. Translation: AAA90951.1.
    U29875 Genomic DNA. Translation: AAA90952.1.
    S76459 mRNA. Translation: AAB33069.1.
    S76461 mRNA. Translation: AAB33070.1.
    S76464 mRNA. Translation: AAB33071.1.
    U44024 Genomic DNA. Translation: AAA93307.1.
    U44024 Genomic DNA. Translation: AAA93306.1.
    AK020105 mRNA. Translation: BAB31997.1.
    CCDSiCCDS39948.1. [P49772-1]
    PIRiA49265.
    I58343.
    RefSeqiNP_038548.3. NM_013520.3. [P49772-1]
    UniGeneiMm.1658.

    Genome annotation databases

    EnsembliENSMUST00000085377; ENSMUSP00000082492; ENSMUSG00000089989. [P49772-1]
    ENSMUST00000123381; ENSMUSP00000115222; ENSMUSG00000089989. [P49772-1]
    ENSMUST00000125500; ENSMUSP00000119175; ENSMUSG00000089989. [P49772-4]
    ENSMUST00000130628; ENSMUSP00000118193; ENSMUSG00000089989. [P49772-2]
    ENSMUST00000146760; ENSMUSP00000123506; ENSMUSG00000089989. [P49772-1]
    GeneIDi14256.
    KEGGimmu:14256.
    UCSCiuc009gtn.2. mouse. [P49772-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04807 mRNA. Translation: AAA18000.1 .
    L23636 mRNA. Translation: AAA39436.1 .
    U29875 Genomic DNA. Translation: AAA90951.1 .
    U29875 Genomic DNA. Translation: AAA90952.1 .
    S76459 mRNA. Translation: AAB33069.1 .
    S76461 mRNA. Translation: AAB33070.1 .
    S76464 mRNA. Translation: AAB33071.1 .
    U44024 Genomic DNA. Translation: AAA93307.1 .
    U44024 Genomic DNA. Translation: AAA93306.1 .
    AK020105 mRNA. Translation: BAB31997.1 .
    CCDSi CCDS39948.1. [P49772-1 ]
    PIRi A49265.
    I58343.
    RefSeqi NP_038548.3. NM_013520.3. [P49772-1 ]
    UniGenei Mm.1658.

    3D structure databases

    ProteinModelPortali P49772.
    SMRi P49772. Positions 30-162.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P49772.
    PRIDEi P49772.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000085377 ; ENSMUSP00000082492 ; ENSMUSG00000089989 . [P49772-1 ]
    ENSMUST00000123381 ; ENSMUSP00000115222 ; ENSMUSG00000089989 . [P49772-1 ]
    ENSMUST00000125500 ; ENSMUSP00000119175 ; ENSMUSG00000089989 . [P49772-4 ]
    ENSMUST00000130628 ; ENSMUSP00000118193 ; ENSMUSG00000089989 . [P49772-2 ]
    ENSMUST00000146760 ; ENSMUSP00000123506 ; ENSMUSG00000089989 . [P49772-1 ]
    GeneIDi 14256.
    KEGGi mmu:14256.
    UCSCi uc009gtn.2. mouse. [P49772-1 ]

    Organism-specific databases

    CTDi 14256.
    MGIi MGI:95560. Flt3l.

    Phylogenomic databases

    eggNOGi COG0102.
    GeneTreei ENSGT00530000064424.
    HOGENOMi HOG000029239.
    HOVERGENi HBG051630.
    KOi K05454.
    OMAi AVNTEIH.
    OrthoDBi EOG7HTHJG.
    PhylomeDBi P49772.
    TreeFami TF338225.

    Miscellaneous databases

    NextBioi 285587.
    PROi P49772.
    SOURCEi Search...

    Gene expression databases

    Bgeei P49772.
    CleanExi MM_FLT3L.
    Genevestigatori P49772.

    Family and domain databases

    Gene3Di 1.20.1250.10. 2 hits.
    InterProi IPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR004213. Flt3_lig.
    [Graphical view ]
    PANTHERi PTHR11032. PTHR11032. 1 hit.
    Pfami PF02947. Flt3_lig. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47266. SSF47266. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular cloning of a ligand for the flt3/flk-2 tyrosine kinase receptor: a proliferative factor for primitive hematopoietic cells."
      Lyman S.D., James L., Vanden Bos T., Devries P., Brasel K., Gliniak B., Hollingsworth L.T., Picha K.S., McKenna H.J., Splett R.R., Fletcher F.A., Maraskovsky E., Farrah T., Foxworthe D., Williams D.E., Beckmann M.P.
      Cell 75:1157-1167(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: SJL/J.
    3. "Structural analysis of human and murine flt3 ligand genomic loci."
      Lyman S.D., Stocking K., Davison B., Fletcher F., Johnson L., Escobar S.
      Oncogene 11:1165-1172(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
    4. "Identification of soluble and membrane-bound isoforms of the murine flt3 ligand generated by alternative splicing of mRNAs."
      Lyman S.D., James L., Escobar S., Downey H., de Vries P., Brasel K., Stocking K., Beckmann M.P., Copeland N.G., Cleveland L.S.
      Oncogene 10:149-157(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Embryo.

    Entry informationi

    Entry nameiFLT3L_MOUSE
    AccessioniPrimary (citable) accession number: P49772
    Secondary accession number(s): Q64085
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3