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P49772 (FLT3L_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fms-related tyrosine kinase 3 ligand

Short name=Flt3 ligand
Short name=Flt3L
Alternative name(s):
SL cytokine
Gene names
Name:Flt3lg
Synonyms:Flt3l
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length232 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

Subunit structure

Homodimer (soluble isoform) By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein.

Isoform 3: Secreted. Note: Biologically active.

Isoform 4: Secreted. Note: Inactive.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P49772-1)

Also known as: 6C;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P49772-2)

Also known as: 5H;

The sequence of this isoform differs from the canonical sequence as follows:
     164-232: DSSTLLPPRS...HPGVPLPSHP → GNGGPRAQHH...SSFLKMYLYV
Isoform 3 (identifier: P49772-3)

Also known as: E6;

The sequence of this isoform differs from the canonical sequence as follows:
     164-169: DSSTLL → GSHYAG
     170-232: Missing.
Isoform 4 (identifier: P49772-4)

Also known as: E6Delta16;

The sequence of this isoform differs from the canonical sequence as follows:
     159-163: VQCQP → GLIMQARLTLNSKQSSCLSVLSAGIT

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 232206Fms-related tyrosine kinase 3 ligand
PRO_0000021282

Regions

Topological domain27 – 189163Extracellular Potential
Transmembrane190 – 21021Helical; Potential
Topological domain211 – 23222Cytoplasmic Potential

Amino acid modifications

Glycosylation1271N-linked (GlcNAc...) Potential
Glycosylation1521N-linked (GlcNAc...) Potential
Disulfide bond31 ↔ 112 By similarity
Disulfide bond71 ↔ 156 By similarity
Disulfide bond120 ↔ 161 By similarity

Natural variations

Alternative sequence159 – 1635VQCQP → GLIMQARLTLNSKQSSCLSV LSAGIT in isoform 4.
VSP_004256
Alternative sequence164 – 23269DSSTL…LPSHP → GNGGPRAQHHGATRLTATAL LTVCPGLLLPLVGTSHMFFL PYFLSFLSSFLKMYLYV in isoform 2.
VSP_004253
Alternative sequence164 – 1696DSSTLL → GSHYAG in isoform 3.
VSP_004254
Alternative sequence170 – 23263Missing in isoform 3.
VSP_004255

Experimental info

Sequence conflict1411A → G in AAA18000. Ref.1
Sequence conflict1981Missing Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (6C) [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 3A3680D3CB69FBA6

FASTA23226,141
        10         20         30         40         50         60 
MTVLAPAWSP NSSLLLLLLL LSPCLRGTPD CYFSHSPISS NFKVKFRELT DHLLKDYPVT 

        70         80         90        100        110        120 
VAVNLQDEKH CKALWSLFLA QRWIEQLKTV AGSKMQTLLE DVNTEIHFVT SCTFQPLPEC 

       130        140        150        160        170        180 
LRFVQTNISH LLKDTCTQLL ALKPCIGKAC QNFSRCLEVQ CQPDSSTLLP PRSPIALEAT 

       190        200        210        220        230 
ELPEPRPRQL LLLLLLLLPL TLVLLAAAWG LRWQRARRRG ELHPGVPLPS HP 

« Hide

Isoform 2 (5H) [UniParc].

Checksum: 8D2FB35D2A706E53
Show »

FASTA22024,674
Isoform 3 (E6) [UniParc].

Checksum: A6DDE0C0A5F23623
Show »

FASTA16919,037
Isoform 4 (E6Delta16) [UniParc].

Checksum: 92164080DC647D99
Show »

FASTA25328,259

References

« Hide 'large scale' references
[1]"Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs."
Hannum C., Culpepper J., Campbell D., McClanahan T., Zurawski S., Bazan J.F., Kastelein R., Hudak S., Wagner J., Mattson J., Luh J., Duda G., Martina N., Peterson D., Menon S., Shanafelt A., Muench M., Kelner G. expand/collapse author list , Namikawa R., Rennick D., Roncarolo M.G., Zlotnik A., Rosnet O., Dubreuil P., Birnbaum D., Lee F.
Nature 368:643-648(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular cloning of a ligand for the flt3/flk-2 tyrosine kinase receptor: a proliferative factor for primitive hematopoietic cells."
Lyman S.D., James L., Vanden Bos T., Devries P., Brasel K., Gliniak B., Hollingsworth L.T., Picha K.S., McKenna H.J., Splett R.R., Fletcher F.A., Maraskovsky E., Farrah T., Foxworthe D., Williams D.E., Beckmann M.P.
Cell 75:1157-1167(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: SJL/J.
[3]"Structural analysis of human and murine flt3 ligand genomic loci."
Lyman S.D., Stocking K., Davison B., Fletcher F., Johnson L., Escobar S.
Oncogene 11:1165-1172(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
[4]"Identification of soluble and membrane-bound isoforms of the murine flt3 ligand generated by alternative splicing of mRNAs."
Lyman S.D., James L., Escobar S., Downey H., de Vries P., Brasel K., Stocking K., Beckmann M.P., Copeland N.G., Cleveland L.S.
Oncogene 10:149-157(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[5]McClanahan T., Culpepper J., Campbell D., Wagner J., Franz-Bacon K., Mattson J., Tsai S., Luh J., Guimares M.J., Mattei M.-G., Rosnet O., Birnbaum D., Hannum C.
Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U04807 mRNA. Translation: AAA18000.1.
L23636 mRNA. Translation: AAA39436.1.
U29875 Genomic DNA. Translation: AAA90951.1.
U29875 Genomic DNA. Translation: AAA90952.1.
S76459 mRNA. Translation: AAB33069.1.
S76461 mRNA. Translation: AAB33070.1.
S76464 mRNA. Translation: AAB33071.1.
U44024 Genomic DNA. Translation: AAA93307.1.
U44024 Genomic DNA. Translation: AAA93306.1.
AK020105 mRNA. Translation: BAB31997.1.
PIRA49265.
I58343.
RefSeqNP_038548.3. NM_013520.3.
UniGeneMm.1658.

3D structure databases

ProteinModelPortalP49772.
SMRP49772. Positions 30-162.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP49772.
PRIDEP49772.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000085377; ENSMUSP00000082492; ENSMUSG00000089989. [P49772-1]
ENSMUST00000123381; ENSMUSP00000115222; ENSMUSG00000089989. [P49772-1]
ENSMUST00000125500; ENSMUSP00000119175; ENSMUSG00000089989. [P49772-4]
ENSMUST00000130628; ENSMUSP00000118193; ENSMUSG00000089989. [P49772-2]
ENSMUST00000146760; ENSMUSP00000123506; ENSMUSG00000089989. [P49772-1]
GeneID14256.
KEGGmmu:14256.
UCSCuc009gtn.2. mouse. [P49772-1]

Organism-specific databases

CTD14256.
MGIMGI:95560. Flt3l.

Phylogenomic databases

eggNOGCOG0102.
GeneTreeENSGT00530000064424.
HOGENOMHOG000029239.
HOVERGENHBG051630.
KOK05454.
OMAAVNTEIH.
OrthoDBEOG7HTHJG.
PhylomeDBP49772.
TreeFamTF338225.

Gene expression databases

BgeeP49772.
CleanExMM_FLT3L.
GenevestigatorP49772.

Family and domain databases

Gene3D1.20.1250.10. 2 hits.
InterProIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR004213. Flt3_lig.
[Graphical view]
PANTHERPTHR11032. PTHR11032. 1 hit.
PfamPF02947. Flt3_lig. 1 hit.
[Graphical view]
SUPFAMSSF47266. SSF47266. 1 hit.
ProtoNetSearch...

Other

NextBio285587.
PROP49772.
SOURCESearch...

Entry information

Entry nameFLT3L_MOUSE
AccessionPrimary (citable) accession number: P49772
Secondary accession number(s): Q64085
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot