P49771 (FLT3L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fms-related tyrosine kinase 3 ligand
Short name=Flt3 ligand
Short name=Flt3L

Alternative name(s):
SL cytokine

Gene names

Name:

FLT3LG

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Subunit structure	Homodimer (isoform 2).
Subcellular location	Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	embryonic hemopoiesis Inferred from direct assay PubMed 21149635. Source: DFLAT positive regulation of cell proliferation Inferred from direct assay Ref.2. Source: UniProtKB signal transduction Traceable author statement Ref.2. Source: ProtInc
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Non-traceable author statement Ref.2. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	receptor binding Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P49771-1)

Also known as: Membrane-bound;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P49771-2)

Also known as: Soluble;

The sequence of this isoform differs from the canonical sequence as follows:
     161-178: DSSTLPPPWSPRPLEATA → VETVFHRVSQDGLDLLTS
     179-235: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

26

Potential

Chain

27 – 235

209

Fms-related tyrosine kinase 3 ligand

PRO_0000021281

Regions

Topological domain

27 – 184

158

Extracellular Potential

Transmembrane

185 – 205

21

Helical; Potential

Topological domain

206 – 235

30

Cytoplasmic Potential

Amino acid modifications

Glycosylation

126

1

N-linked (GlcNAc...) Potential

Glycosylation

149

1

N-linked (GlcNAc...) Potential

Disulfide bond

30 ↔ 111

Disulfide bond

70 ↔ 153

Disulfide bond

119 ↔ 158

Natural variations

Alternative sequence

161 – 178

18

DSSTL…LEATA → VETVFHRVSQDGLDLLTS in isoform 2.

VSP_004251

Alternative sequence

179 – 235

57

Missing in isoform 2.

VSP_004252

Experimental info

Sequence conflict

72

1

G → A in AAA17999. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

235

Helix Strand Turn

Details...

Helix

41 – 49

9

Beta strand

57 – 62

6

Turn

68 – 70

3

Helix

71 – 87

17

Helix

92 – 105

14

Helix

106 – 110

5

Beta strand

122 – 126

5

Helix

127 – 141

15

Turn

142 – 147

6

Helix

151 – 153

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Membrane-bound) [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 73B95BF693B4CECF	FASTA	235	26,416
	10 20 30 40 50 60 MTVLAPAWSP TTYLLLLLLL SSGLSGTQDC SFQHSPISSD FAVKIRELSD YLLQDYPVTV 70 80 90 100 110 120 ASNLQDEELC GGLWRLVLAQ RWMERLKTVA GSKMQGLLER VNTEIHFVTK CAFQPPPSCL 130 140 150 160 170 180 RFVQTNISRL LQETSEQLVA LKPWITRQNF SRCLELQCQP DSSTLPPPWS PRPLEATAPT 190 200 210 220 230 APQPPLLLLL LLPVGLLLLA AAWCLHWQRT RRRTPRPGEQ VPPVPSPQDL LLVEH
	Isoform 2 (Soluble) [UniParc]. Checksum: D9E7D4C5FBD370D8	FASTA	178	20,142
	10 20 30 40 50 60 MTVLAPAWSP TTYLLLLLLL SSGLSGTQDC SFQHSPISSD FAVKIRELSD YLLQDYPVTV 70 80 90 100 110 120 ASNLQDEELC GGLWRLVLAQ RWMERLKTVA GSKMQGLLER VNTEIHFVTK CAFQPPPSCL 130 140 150 160 170 RFVQTNISRL LQETSEQLVA LKPWITRQNF SRCLELQCQP VETVFHRVSQ DGLDLLTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs." Hannum C., Culpepper J., Campbell D., McClanahan T., Zurawski S., Bazan J.F., Kastelein R., Hudak S., Wagner J., Mattson J., Luh J., Duda G., Martina N., Peterson D., Menon S., Shanafelt A., Muench M., Kelner G. , Namikawa R., Rennick D., Roncarolo M.G., Zlotnik A., Rosnet O., Dubreuil P., Birnbaum D., Lee F. Nature 368:643-648(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Cloning of the human homologue of the murine flt3 ligand: a growth factor for early hematopoietic progenitor cells." Lyman S.D., James L., Johnson L., Brasel K., de Vries P., Escobar S.S., Downey H., Splett R.R., Beckmann M.P., McKenna H.J. Blood 83:2795-2801(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Structural analysis of human and murine flt3 ligand genomic loci." Lyman S.D., Stocking K., Davison B., Fletcher F., Johnson L., Escobar S. Oncogene 11:1165-1172(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Flt3 ligand structure and unexpected commonalities of helical bundles and cystine knots." Savvides S.N., Boone T., Karplus P.A. Nat. Struct. Biol. 7:486-491(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U04806 mRNA. Translation: AAA17999.1.
U03858 mRNA. Translation: AAA19825.1.
U29874 Genomic DNA. Translation: AAA90949.1.
U29874 Genomic DNA. Translation: AAA90950.1.
BC126293 mRNA. Translation: AAI26294.1.
BC136464 mRNA. Translation: AAI36465.1.

IPI

IPI00216372.
IPI00291404.

PIR

I38440.
I39076.

RefSeq

NP_001191431.1. NM_001204502.1.
NP_001191432.1. NM_001204503.1.
NP_001450.2. NM_001459.3.

UniGene

Hs.428.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ETE	X-ray	2.20	A/B/C/D	27-160	[»]
3QS7	X-ray	4.30	A/B/C/D	27-160	[»]
3QS9	X-ray	7.80	A/B/C/D	27-160	[»]

ProteinModelPortal

P49771.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6220N.

IntAct

P49771. 1 interaction.

MINT

MINT-1371383.

STRING

9606.ENSP00000204637.

Polymorphism databases

DMDM

1706818.

Proteomic databases

PRIDE

P49771.

Protocols and materials databases

DNASU

2323.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000344019; ENSP00000341305; ENSG00000090554.
ENST00000594009; ENSP00000469613; ENSG00000090554.
ENST00000597551; ENSP00000468977; ENSG00000090554.
ENST00000600429; ENSP00000470453; ENSG00000090554.

GeneID

2323.

KEGG

hsa:2323.

UCSC

uc002pnu.3. human.

Organism-specific databases

CTD

2323.

GeneCards

GC19P049977.

H-InvDB

HIX0202990.

HGNC

HGNC:3766. FLT3LG.

HPA

HPA017313.

MIM

600007. gene.

neXtProt

NX_P49771.

PharmGKB

PA28182.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG44664.

HOGENOM

HOG000029239.

HOVERGEN

HBG051630.

InParanoid

P49771.

KO

K05454.

OMA

AVNTEIH.

OrthoDB

EOG4H4654.

PhylomeDB

P49771.

Gene expression databases

ArrayExpress

P49771.

Bgee

P49771.

CleanEx

HS_FLT3LG.

Genevestigator

P49771.

GermOnline

ENSG00000090554. Homo sapiens.

Family and domain databases

Gene3D

1.20.1250.10. 2 hits.

InterPro

IPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR004213. Flt3_lig.
[Graphical view]

PANTHER

PTHR11032. PTHR11032. 1 hit.

Pfam

PF02947. Flt3_lig. 1 hit.
[Graphical view]

SUPFAM

SSF47266. 4_helix_cytokine. 1 hit.

ProtoNet

Search...

Other

ChiTaRS

FLT3LG. human.

EvolutionaryTrace

P49771.

GenomeRNAi

2323.

NextBio

9429.

SOURCE

Search...

Entry information

Entry name

FLT3L_HUMAN

Accession

Primary (citable) accession number: P49771
Secondary accession number(s): A0AVC2, B9EGH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.