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P49771

- FLT3L_HUMAN

UniProt

P49771 - FLT3L_HUMAN

Protein

Fms-related tyrosine kinase 3 ligand

Gene

FLT3LG

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

    GO - Molecular functioni

    1. receptor binding Source: ProtInc

    GO - Biological processi

    1. embryonic hemopoiesis Source: DFLAT
    2. lymphocyte differentiation Source: Ensembl
    3. positive regulation of cell proliferation Source: UniProtKB
    4. positive regulation of natural killer cell differentiation Source: Ensembl
    5. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    Cytokine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fms-related tyrosine kinase 3 ligand
    Short name:
    Flt3 ligand
    Short name:
    Flt3L
    Alternative name(s):
    SL cytokine
    Gene namesi
    Name:FLT3LG
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:3766. FLT3LG.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-KW
    2. integral component of membrane Source: UniProtKB
    3. intrinsic component of external side of plasma membrane Source: Ensembl
    4. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28182.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 235209Fms-related tyrosine kinase 3 ligandPRO_0000021281Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi30 ↔ 111
    Disulfide bondi70 ↔ 153
    Disulfide bondi119 ↔ 158
    Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP49771.

    Expressioni

    Gene expression databases

    ArrayExpressiP49771.
    BgeeiP49771.
    CleanExiHS_FLT3LG.
    GenevestigatoriP49771.

    Organism-specific databases

    HPAiHPA017313.

    Interactioni

    Subunit structurei

    Homodimer (isoform 2).

    Protein-protein interaction databases

    BioGridi108611. 1 interaction.
    DIPiDIP-6220N.
    IntActiP49771. 2 interactions.
    MINTiMINT-1371383.
    STRINGi9606.ENSP00000204637.

    Structurei

    Secondary structure

    1
    235
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi41 – 499
    Beta strandi57 – 626
    Turni68 – 703
    Helixi71 – 8717
    Helixi92 – 10514
    Helixi106 – 1105
    Beta strandi122 – 1265
    Helixi127 – 14115
    Turni142 – 1476
    Helixi151 – 1533

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ETEX-ray2.20A/B/C/D27-160[»]
    3QS7X-ray4.30A/B/C/D27-160[»]
    3QS9X-ray7.80A/B/C/D27-160[»]
    ProteinModelPortaliP49771.
    SMRiP49771. Positions 27-159.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP49771.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini27 – 184158ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini206 – 23530CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei185 – 20521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG44664.
    HOGENOMiHOG000029239.
    HOVERGENiHBG051630.
    InParanoidiP49771.
    KOiK05454.
    OMAiAVNTEIH.
    OrthoDBiEOG7HTHJG.
    PhylomeDBiP49771.
    TreeFamiTF338225.

    Family and domain databases

    Gene3Di1.20.1250.10. 2 hits.
    InterProiIPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR004213. Flt3_lig.
    [Graphical view]
    PANTHERiPTHR11032. PTHR11032. 1 hit.
    PfamiPF02947. Flt3_lig. 1 hit.
    [Graphical view]
    SUPFAMiSSF47266. SSF47266. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P49771-1) [UniParc]FASTAAdd to Basket

    Also known as: Membrane-bound

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTVLAPAWSP TTYLLLLLLL SSGLSGTQDC SFQHSPISSD FAVKIRELSD    50
    YLLQDYPVTV ASNLQDEELC GGLWRLVLAQ RWMERLKTVA GSKMQGLLER 100
    VNTEIHFVTK CAFQPPPSCL RFVQTNISRL LQETSEQLVA LKPWITRQNF 150
    SRCLELQCQP DSSTLPPPWS PRPLEATAPT APQPPLLLLL LLPVGLLLLA 200
    AAWCLHWQRT RRRTPRPGEQ VPPVPSPQDL LLVEH 235
    Length:235
    Mass (Da):26,416
    Last modified:October 1, 1996 - v1
    Checksum:i73B95BF693B4CECF
    GO
    Isoform 2 (identifier: P49771-2) [UniParc]FASTAAdd to Basket

    Also known as: Soluble

    The sequence of this isoform differs from the canonical sequence as follows:
         161-178: DSSTLPPPWSPRPLEATA → VETVFHRVSQDGLDLLTS
         179-235: Missing.

    Show »
    Length:178
    Mass (Da):20,142
    Checksum:iD9E7D4C5FBD370D8
    GO
    Isoform 3 (identifier: P49771-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-82: Missing.

    Show »
    Length:153
    Mass (Da):17,290
    Checksum:i84FB972427691E35
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti72 – 721G → A in AAA17999. (PubMed:8145851)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 8282Missing in isoform 3. 2 PublicationsVSP_054599Add
    BLAST
    Alternative sequencei161 – 17818DSSTL…LEATA → VETVFHRVSQDGLDLLTS in isoform 2. CuratedVSP_004251Add
    BLAST
    Alternative sequencei179 – 23557Missing in isoform 2. CuratedVSP_004252Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04806 mRNA. Translation: AAA17999.1.
    U03858 mRNA. Translation: AAA19825.1.
    U29874 Genomic DNA. Translation: AAA90949.1.
    U29874 Genomic DNA. Translation: AAA90950.1.
    AK301136 mRNA. Translation: BAG62728.1.
    AC010619 Genomic DNA. No translation available.
    BC028001 mRNA. Translation: AAH28001.1.
    BC126293 mRNA. Translation: AAI26294.1.
    BC136464 mRNA. Translation: AAI36465.1.
    CCDSiCCDS12767.1. [P49771-1]
    CCDS62753.1. [P49771-3]
    PIRiI38440.
    I39076.
    RefSeqiNP_001191431.1. NM_001204502.1. [P49771-1]
    NP_001191432.1. NM_001204503.1. [P49771-1]
    NP_001265566.1. NM_001278637.1. [P49771-3]
    NP_001265567.1. NM_001278638.1. [P49771-3]
    NP_001450.2. NM_001459.3. [P49771-1]
    UniGeneiHs.428.

    Genome annotation databases

    EnsembliENST00000204637; ENSP00000204637; ENSG00000090554. [P49771-3]
    ENST00000594009; ENSP00000469613; ENSG00000090554. [P49771-1]
    ENST00000595510; ENSP00000471226; ENSG00000090554. [P49771-3]
    ENST00000597551; ENSP00000468977; ENSG00000090554. [P49771-1]
    ENST00000600429; ENSP00000470453; ENSG00000090554. [P49771-1]
    GeneIDi2323.
    KEGGihsa:2323.
    UCSCiuc002pnu.3. human. [P49771-1]

    Polymorphism databases

    DMDMi1706818.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04806 mRNA. Translation: AAA17999.1 .
    U03858 mRNA. Translation: AAA19825.1 .
    U29874 Genomic DNA. Translation: AAA90949.1 .
    U29874 Genomic DNA. Translation: AAA90950.1 .
    AK301136 mRNA. Translation: BAG62728.1 .
    AC010619 Genomic DNA. No translation available.
    BC028001 mRNA. Translation: AAH28001.1 .
    BC126293 mRNA. Translation: AAI26294.1 .
    BC136464 mRNA. Translation: AAI36465.1 .
    CCDSi CCDS12767.1. [P49771-1 ]
    CCDS62753.1. [P49771-3 ]
    PIRi I38440.
    I39076.
    RefSeqi NP_001191431.1. NM_001204502.1. [P49771-1 ]
    NP_001191432.1. NM_001204503.1. [P49771-1 ]
    NP_001265566.1. NM_001278637.1. [P49771-3 ]
    NP_001265567.1. NM_001278638.1. [P49771-3 ]
    NP_001450.2. NM_001459.3. [P49771-1 ]
    UniGenei Hs.428.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ETE X-ray 2.20 A/B/C/D 27-160 [» ]
    3QS7 X-ray 4.30 A/B/C/D 27-160 [» ]
    3QS9 X-ray 7.80 A/B/C/D 27-160 [» ]
    ProteinModelPortali P49771.
    SMRi P49771. Positions 27-159.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108611. 1 interaction.
    DIPi DIP-6220N.
    IntActi P49771. 2 interactions.
    MINTi MINT-1371383.
    STRINGi 9606.ENSP00000204637.

    Polymorphism databases

    DMDMi 1706818.

    Proteomic databases

    PRIDEi P49771.

    Protocols and materials databases

    DNASUi 2323.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000204637 ; ENSP00000204637 ; ENSG00000090554 . [P49771-3 ]
    ENST00000594009 ; ENSP00000469613 ; ENSG00000090554 . [P49771-1 ]
    ENST00000595510 ; ENSP00000471226 ; ENSG00000090554 . [P49771-3 ]
    ENST00000597551 ; ENSP00000468977 ; ENSG00000090554 . [P49771-1 ]
    ENST00000600429 ; ENSP00000470453 ; ENSG00000090554 . [P49771-1 ]
    GeneIDi 2323.
    KEGGi hsa:2323.
    UCSCi uc002pnu.3. human. [P49771-1 ]

    Organism-specific databases

    CTDi 2323.
    GeneCardsi GC19P049977.
    H-InvDB HIX0202990.
    HGNCi HGNC:3766. FLT3LG.
    HPAi HPA017313.
    MIMi 600007. gene.
    neXtProti NX_P49771.
    PharmGKBi PA28182.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG44664.
    HOGENOMi HOG000029239.
    HOVERGENi HBG051630.
    InParanoidi P49771.
    KOi K05454.
    OMAi AVNTEIH.
    OrthoDBi EOG7HTHJG.
    PhylomeDBi P49771.
    TreeFami TF338225.

    Miscellaneous databases

    ChiTaRSi FLT3LG. human.
    EvolutionaryTracei P49771.
    GeneWikii FLT3LG.
    GenomeRNAii 2323.
    NextBioi 13608020.
    PROi P49771.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P49771.
    Bgeei P49771.
    CleanExi HS_FLT3LG.
    Genevestigatori P49771.

    Family and domain databases

    Gene3Di 1.20.1250.10. 2 hits.
    InterProi IPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR004213. Flt3_lig.
    [Graphical view ]
    PANTHERi PTHR11032. PTHR11032. 1 hit.
    Pfami PF02947. Flt3_lig. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47266. SSF47266. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Cloning of the human homologue of the murine flt3 ligand: a growth factor for early hematopoietic progenitor cells."
      Lyman S.D., James L., Johnson L., Brasel K., de Vries P., Escobar S.S., Downey H., Splett R.R., Beckmann M.P., McKenna H.J.
      Blood 83:2795-2801(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "Structural analysis of human and murine flt3 ligand genomic loci."
      Lyman S.D., Stocking K., Davison B., Fletcher F., Johnson L., Escobar S.
      Oncogene 11:1165-1172(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Spleen.
    5. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Tissue: Brain.
    7. "Flt3 ligand structure and unexpected commonalities of helical bundles and cystine knots."
      Savvides S.N., Boone T., Karplus P.A.
      Nat. Struct. Biol. 7:486-491(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).

    Entry informationi

    Entry nameiFLT3L_HUMAN
    AccessioniPrimary (citable) accession number: P49771
    Secondary accession number(s): A0AVC2, B9EGH2, Q05C96
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3