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P49771 (FLT3L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fms-related tyrosine kinase 3 ligand

Short name=Flt3 ligand
Short name=Flt3L
Alternative name(s):
SL cytokine
Gene names
Name:FLT3LG
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length235 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

Subunit structure

Homodimer (isoform 2)

Subcellular location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Isoform 2: Secreted.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P49771-1)

Also known as: Membrane-bound;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P49771-2)

Also known as: Soluble;

The sequence of this isoform differs from the canonical sequence as follows:
     161-178: DSSTLPPPWSPRPLEATA → VETVFHRVSQDGLDLLTS
     179-235: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 235209Fms-related tyrosine kinase 3 ligand
PRO_0000021281

Regions

Topological domain27 – 184158Extracellular Potential
Transmembrane185 – 20521Helical; Potential
Topological domain206 – 23530Cytoplasmic Potential

Amino acid modifications

Glycosylation1261N-linked (GlcNAc...) Potential
Glycosylation1491N-linked (GlcNAc...) Potential
Disulfide bond30 ↔ 111
Disulfide bond70 ↔ 153
Disulfide bond119 ↔ 158

Natural variations

Alternative sequence161 – 17818DSSTL…LEATA → VETVFHRVSQDGLDLLTS in isoform 2.
VSP_004251
Alternative sequence179 – 23557Missing in isoform 2.
VSP_004252

Experimental info

Sequence conflict721G → A in AAA17999. Ref.1

Secondary structure

................. 235
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Membrane-bound) [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 73B95BF693B4CECF

FASTA23526,416
        10         20         30         40         50         60 
MTVLAPAWSP TTYLLLLLLL SSGLSGTQDC SFQHSPISSD FAVKIRELSD YLLQDYPVTV 

        70         80         90        100        110        120 
ASNLQDEELC GGLWRLVLAQ RWMERLKTVA GSKMQGLLER VNTEIHFVTK CAFQPPPSCL 

       130        140        150        160        170        180 
RFVQTNISRL LQETSEQLVA LKPWITRQNF SRCLELQCQP DSSTLPPPWS PRPLEATAPT 

       190        200        210        220        230 
APQPPLLLLL LLPVGLLLLA AAWCLHWQRT RRRTPRPGEQ VPPVPSPQDL LLVEH 

« Hide

Isoform 2 (Soluble) [UniParc].

Checksum: D9E7D4C5FBD370D8
Show »

FASTA17820,142

References

« Hide 'large scale' references
[1]"Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs."
Hannum C., Culpepper J., Campbell D., McClanahan T., Zurawski S., Bazan J.F., Kastelein R., Hudak S., Wagner J., Mattson J., Luh J., Duda G., Martina N., Peterson D., Menon S., Shanafelt A., Muench M., Kelner G. expand/collapse author list , Namikawa R., Rennick D., Roncarolo M.G., Zlotnik A., Rosnet O., Dubreuil P., Birnbaum D., Lee F.
Nature 368:643-648(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning of the human homologue of the murine flt3 ligand: a growth factor for early hematopoietic progenitor cells."
Lyman S.D., James L., Johnson L., Brasel K., de Vries P., Escobar S.S., Downey H., Splett R.R., Beckmann M.P., McKenna H.J.
Blood 83:2795-2801(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Structural analysis of human and murine flt3 ligand genomic loci."
Lyman S.D., Stocking K., Davison B., Fletcher F., Johnson L., Escobar S.
Oncogene 11:1165-1172(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"Flt3 ligand structure and unexpected commonalities of helical bundles and cystine knots."
Savvides S.N., Boone T., Karplus P.A.
Nat. Struct. Biol. 7:486-491(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U04806 mRNA. Translation: AAA17999.1.
U03858 mRNA. Translation: AAA19825.1.
U29874 Genomic DNA. Translation: AAA90949.1.
U29874 Genomic DNA. Translation: AAA90950.1.
BC126293 mRNA. Translation: AAI26294.1.
BC136464 mRNA. Translation: AAI36465.1.
PIRI38440.
I39076.
RefSeqNP_001191431.1. NM_001204502.1.
NP_001191432.1. NM_001204503.1.
NP_001450.2. NM_001459.3.
UniGeneHs.428.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ETEX-ray2.20A/B/C/D27-160[»]
3QS7X-ray4.30A/B/C/D27-160[»]
3QS9X-ray7.80A/B/C/D27-160[»]
ProteinModelPortalP49771.
SMRP49771. Positions 27-159.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108611. 1 interaction.
DIPDIP-6220N.
IntActP49771. 1 interaction.
MINTMINT-1371383.
STRING9606.ENSP00000204637.

Polymorphism databases

DMDM1706818.

Proteomic databases

PRIDEP49771.

Protocols and materials databases

DNASU2323.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000344019; ENSP00000341305; ENSG00000090554. [P49771-2]
ENST00000594009; ENSP00000469613; ENSG00000090554. [P49771-1]
ENST00000597551; ENSP00000468977; ENSG00000090554. [P49771-1]
ENST00000600429; ENSP00000470453; ENSG00000090554. [P49771-1]
GeneID2323.
KEGGhsa:2323.
UCSCuc002pnu.3. human. [P49771-1]

Organism-specific databases

CTD2323.
GeneCardsGC19P049977.
H-InvDBHIX0202990.
HGNCHGNC:3766. FLT3LG.
HPAHPA017313.
MIM600007. gene.
neXtProtNX_P49771.
PharmGKBPA28182.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG44664.
HOGENOMHOG000029239.
HOVERGENHBG051630.
InParanoidP49771.
KOK05454.
OMAAVNTEIH.
OrthoDBEOG7HTHJG.
PhylomeDBP49771.
TreeFamTF338225.

Gene expression databases

ArrayExpressP49771.
BgeeP49771.
CleanExHS_FLT3LG.
GenevestigatorP49771.

Family and domain databases

Gene3D1.20.1250.10. 2 hits.
InterProIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR004213. Flt3_lig.
[Graphical view]
PANTHERPTHR11032. PTHR11032. 1 hit.
PfamPF02947. Flt3_lig. 1 hit.
[Graphical view]
SUPFAMSSF47266. SSF47266. 1 hit.
ProtoNetSearch...

Other

ChiTaRSFLT3LG. human.
EvolutionaryTraceP49771.
GeneWikiFLT3LG.
GenomeRNAi2323.
NextBio9429.
PROP49771.
SOURCESearch...

Entry information

Entry nameFLT3L_HUMAN
AccessionPrimary (citable) accession number: P49771
Secondary accession number(s): A0AVC2, B9EGH2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM