P49766 (VEGFB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vascular endothelial growth factor B
Short name=VEGF-B

Alternative name(s):
VEGF-related factor
Short name=VRF

Gene names

Name:	Vegfb
Synonyms:	Vrf

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. VEGF-B seems to be required for normal heart function in adult but is not required for proper development of the cardiovascular system either during development or for angiogenesis in adults. Ref.6
Subunit structure	Homodimer; disulfide-linked. Can also form heterodimer with VEGF.
Subcellular location	Secreted. Note: Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
Tissue specificity	Abundantly expressed in heart, brain, kidney and skeletal muscle.
Post-translational modification	VEGF-B186 is O-glycosylated.
Sequence similarities	Belongs to the PDGF/VEGF growth factor family.

Ontologies

Keywords
Biological process	Angiogenesis Differentiation
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Heparin-binding
Molecular function	Developmental protein Growth factor Mitogen
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	angiogenesis Inferred from electronic annotation. Source: UniProtKB-KW cardiac muscle contraction Inferred from mutant phenotype PubMed 10666423. Source: MGI cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW coronary vasculature development Inferred from mutant phenotype PubMed 10666423. Source: MGI induction of positive chemotaxis Inferred from electronic annotation. Source: Compara negative regulation of gene expression Inferred from electronic annotation. Source: Compara negative regulation of neuron apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of ERK1 and ERK2 cascade Inferred from electronic annotation. Source: Compara positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of endothelial cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of mast cell chemotaxis Inferred from electronic annotation. Source: Compara positive regulation of peptidyl-tyrosine phosphorylation Inferred from electronic annotation. Source: Compara positive regulation of protein kinase B signaling cascade Inferred from electronic annotation. Source: Compara positive regulation of vascular endothelial growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of vascular wound healing Inferred from electronic annotation. Source: Compara protein O-linked glycosylation Inferred from direct assay Ref.2. Source: MGI response to drug Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from direct assay Ref.2. Source: MGI extracellular space Inferred from electronic annotation. Source: Compara intracellular Inferred from direct assay Ref.2. Source: MGI membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	chemoattractant activity Inferred from electronic annotation. Source: Compara heparin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform VEGF-B186 (identifier: P49766-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform VEGF-B167 (identifier: P49766-2)

The sequence of this isoform differs from the canonical sequence as follows:
     137-207: RVAIPHHRPQ...AASSIAKGGA → SPRILCPPCT...DTCRCRKPRK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 207	186	Vascular endothelial growth factor B		PRO_0000023399
Amino acid modifications
	Disulfide bond	47 ↔ 89		By similarity
	Disulfide bond	72		Interchain By similarity
	Disulfide bond	78 ↔ 122		By similarity
	Disulfide bond	81		Interchain By similarity
	Disulfide bond	82 ↔ 124		By similarity
Natural variations
	Alternative sequence	137 – 207	71	RVAIP…AKGGA → SPRILCPPCTQRRQRPDPRT CRCRCRRRRFLHCQGRGLEL NPDTCRCRKPRK in isoform VEGF-B167.		VSP_004641
Experimental info
	Sequence conflict	69	1	V → A in BAE28405. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform VEGF-B186 [UniParc]. Last modified November 16, 2001. Version 2. Checksum: 4817A5E96F6960C2	FASTA	207	21,914
	10 20 30 40 50 60 MSPLLRRLLL VALLQLARTQ APVSQFDGPS HQKKVVPWID VYARATCQPR EVVVPLSMEL 70 80 90 100 110 120 MGNVVKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIQYPSSQ LGEMSLEEHS 130 140 150 160 170 180 QCECRPKKKE SAVKPDRVAI PHHRPQPRSV PGWDSTPGAS SPADIIHPTP APGSSARLAP 190 200 SAVNALTPGP AAAAADAAAS SIAKGGA
	Isoform VEGF-B167 [UniParc]. Checksum: D52A055FB995E9CA	FASTA	188	21,442
	10 20 30 40 50 60 MSPLLRRLLL VALLQLARTQ APVSQFDGPS HQKKVVPWID VYARATCQPR EVVVPLSMEL 70 80 90 100 110 120 MGNVVKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIQYPSSQ LGEMSLEEHS 130 140 150 160 170 180 QCECRPKKKE SAVKPDSPRI LCPPCTQRRQ RPDPRTCRCR CRRRRFLHCQ GRGLELNPDT CRCRKPRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the murine VEGF-related factor gene." Townson S., Lagercrantz J., Grimmond S., Silins G., Nordenskjoeld M., Weber G., Hayward N.K. Biochem. Biophys. Res. Commun. 220:922-928(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-B186 AND VEGF-B167). Tissue: Brain.
[2]	"Genomic organization of the mouse and human genes for vascular endothelial growth factor B (VEGF-B) and characterization of a second splice isoform." Olofsson B., Pajusola K., von Euler G., Chilov D., Alitalo K., Eriksson U. J. Biol. Chem. 271:19310-19317(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-B186). Tissue: Heart.
[3]	"Vascular endothelial growth factor B, a novel growth factor for endothelial cells." Olofsson B., Pajusola K., Kaipainen A., von Euler G., Joukov V., Saksela O., Orpana A., Pettersson R.F., Alitalo K., Eriksson U. Proc. Natl. Acad. Sci. U.S.A. 93:2576-2581(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-B167). Tissue: Heart.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM VEGF-B186). Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM VEGF-B167). Tissue: Eye.
[6]	"Vascular endothelial growth factor-B-deficient mice display an atrial conduction defect." Aase K., von Euler G., Li X., Ponten A., Thoren P., Cao R., Cao Y., Olofsson B., Gebre-Medhin S., Pekny M., Alitalo K., Betsholtz C., Eriksson U. Circulation 104:358-364(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U43836 mRNA. Translation: AAC52932.1. U43837 mRNA. Translation: AAC52553.1. U52820 mRNA. Translation: AAC52823.1. U48800 mRNA. Translation: AAB06273.1. AK148188 mRNA. Translation: BAE28405.1. BC046303 mRNA. Translation: AAH46303.1.
IPI	IPI00117120. IPI00230097.
PIR	JC4679. JC4680.
RefSeq	NP_001172093.1. NM_001185164.1. NP_035827.1. NM_011697.3.
UniGene	Mm.15607.
3D structure databases
ProteinModelPortal	P49766.
SMR	P49766. Positions 34-128.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49766. 1 interaction.
Proteomic databases
PRIDE	P49766.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000025914; ENSMUSP00000025914; ENSMUSG00000024962. ENSMUST00000130048; ENSMUSP00000120860; ENSMUSG00000024962.
GeneID	22340.
KEGG	mmu:22340.
UCSC	uc008gjv.2. mouse. uc008gjw.2. mouse.
Organism-specific databases
CTD	7423.
MGI	MGI:106199. Vegfb.
Phylogenomic databases
eggNOG	NOG77417.
GeneTree	ENSGT00530000062930.
HOGENOM	HOG000230896.
HOVERGEN	HBG000105.
InParanoid	P49766.
KO	K16858.
OMA	ASTPHHR.
OrthoDB	EOG4PNXJH.
Gene expression databases
Bgee	P49766.
CleanEx	MM_VEGFB.
Genevestigator	P49766.
GermOnline	ENSMUSG00000024962. Mus musculus.
Family and domain databases
InterPro	IPR000072. PD_growth_factor. IPR023581. PD_growth_factor_CS. [Graphical view]
Pfam	PF00341. PDGF. 1 hit. [Graphical view]
SMART	SM00141. PDGF. 1 hit. [Graphical view]
PROSITE	PS00249. PDGF_1. 1 hit. PS50278. PDGF_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302601.
SOURCE	Search...

Entry information

Entry name

VEGFB_MOUSE

Accession

Primary (citable) accession number: P49766
Secondary accession number(s): Q3UG04, Q5D0B1, Q64290

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 16, 2001
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program