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Protein

Vascular endothelial growth factor B

Gene

Vegfb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. VEGF-B seems to be required for normal heart function in adult but is not required for proper development of the cardiovascular system either during development or for angiogenesis in adults.1 Publication

GO - Molecular functioni

  1. chemoattractant activity Source: MGI
  2. heparin binding Source: UniProtKB-KW
  3. protein heterodimerization activity Source: MGI
  4. protein homodimerization activity Source: MGI
  5. vascular endothelial growth factor receptor 1 binding Source: MGI

GO - Biological processi

  1. angiogenesis Source: UniProtKB-KW
  2. cardiac muscle contraction Source: MGI
  3. cell differentiation Source: UniProtKB-KW
  4. coronary vasculature development Source: MGI
  5. heart development Source: MGI
  6. induction of positive chemotaxis Source: MGI
  7. negative regulation of apoptotic process Source: MGI
  8. negative regulation of gene expression Source: MGI
  9. negative regulation of neuron apoptotic process Source: MGI
  10. positive chemotaxis Source: MGI
  11. positive regulation of cell division Source: UniProtKB-KW
  12. positive regulation of endothelial cell proliferation Source: MGI
  13. positive regulation of ERK1 and ERK2 cascade Source: MGI
  14. positive regulation of mast cell chemotaxis Source: MGI
  15. positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  16. positive regulation of protein kinase B signaling Source: MGI
  17. positive regulation of vascular endothelial growth factor receptor signaling pathway Source: MGI
  18. positive regulation of vascular wound healing Source: MGI
  19. protein O-linked glycosylation Source: MGI
  20. response to drug Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Growth factor, Mitogen

Keywords - Biological processi

Angiogenesis, Differentiation

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiREACT_293283. VEGF binds to VEGFR leading to receptor dimerization.
REACT_307071. Platelet degranulation.
REACT_343634. VEGF ligand-receptor interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Vascular endothelial growth factor B
Short name:
VEGF-B
Alternative name(s):
VEGF-related factor
Short name:
VRF
Gene namesi
Name:Vegfb
Synonyms:Vrf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:106199. Vegfb.

Subcellular locationi

Secreted
Note: Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

GO - Cellular componenti

  1. extracellular region Source: MGI
  2. extracellular space Source: MGI
  3. intracellular Source: MGI
  4. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 207186Vascular endothelial growth factor BPRO_0000023399Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi47 ↔ 89By similarity
Disulfide bondi72 – 72InterchainBy similarity
Disulfide bondi78 ↔ 122By similarity
Disulfide bondi81 – 81InterchainBy similarity
Disulfide bondi82 ↔ 124By similarity

Post-translational modificationi

VEGF-B186 is O-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP49766.

Expressioni

Tissue specificityi

Abundantly expressed in heart, brain, kidney and skeletal muscle.

Gene expression databases

BgeeiP49766.
CleanExiMM_VEGFB.
GenevestigatoriP49766.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Can also form heterodimer with VEGF.

Protein-protein interaction databases

IntActiP49766. 1 interaction.
MINTiMINT-1573475.

Structurei

3D structure databases

ProteinModelPortaliP49766.
SMRiP49766. Positions 34-128.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PDGF/VEGF growth factor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG77417.
GeneTreeiENSGT00730000110791.
HOGENOMiHOG000230896.
HOVERGENiHBG000105.
InParanoidiP49766.
KOiK16858.
OMAiASTPHHR.
OrthoDBiEOG74R1S0.
PhylomeDBiP49766.
TreeFamiTF319554.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
[Graphical view]
PfamiPF00341. PDGF. 1 hit.
[Graphical view]
SMARTiSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform VEGF-B186 (identifier: P49766-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSPLLRRLLL VALLQLARTQ APVSQFDGPS HQKKVVPWID VYARATCQPR
60 70 80 90 100
EVVVPLSMEL MGNVVKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ
110 120 130 140 150
ILMIQYPSSQ LGEMSLEEHS QCECRPKKKE SAVKPDRVAI PHHRPQPRSV
160 170 180 190 200
PGWDSTPGAS SPADIIHPTP APGSSARLAP SAVNALTPGP AAAAADAAAS

SIAKGGA
Length:207
Mass (Da):21,914
Last modified:November 15, 2001 - v2
Checksum:i4817A5E96F6960C2
GO
Isoform VEGF-B167 (identifier: P49766-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     137-207: RVAIPHHRPQ...AASSIAKGGA → SPRILCPPCT...DTCRCRKPRK

Show »
Length:188
Mass (Da):21,442
Checksum:iD52A055FB995E9CA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti69 – 691V → A in BAE28405 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei137 – 20771RVAIP…AKGGA → SPRILCPPCTQRRQRPDPRT CRCRCRRRRFLHCQGRGLEL NPDTCRCRKPRK in isoform VEGF-B167. 3 PublicationsVSP_004641Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43836 mRNA. Translation: AAC52932.1.
U43837 mRNA. Translation: AAC52553.1.
U52820 mRNA. Translation: AAC52823.1.
U48800 mRNA. Translation: AAB06273.1.
AK148188 mRNA. Translation: BAE28405.1.
BC046303 mRNA. Translation: AAH46303.1.
CCDSiCCDS29516.1. [P49766-1]
CCDS50373.1. [P49766-2]
PIRiJC4679.
JC4680.
RefSeqiNP_001172093.1. NM_001185164.1. [P49766-2]
NP_035827.1. NM_011697.3. [P49766-1]
UniGeneiMm.15607.

Genome annotation databases

EnsembliENSMUST00000025914; ENSMUSP00000025914; ENSMUSG00000024962. [P49766-1]
ENSMUST00000130048; ENSMUSP00000120860; ENSMUSG00000024962. [P49766-2]
GeneIDi22340.
KEGGimmu:22340.
UCSCiuc008gjv.2. mouse. [P49766-1]
uc008gjw.2. mouse. [P49766-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43836 mRNA. Translation: AAC52932.1.
U43837 mRNA. Translation: AAC52553.1.
U52820 mRNA. Translation: AAC52823.1.
U48800 mRNA. Translation: AAB06273.1.
AK148188 mRNA. Translation: BAE28405.1.
BC046303 mRNA. Translation: AAH46303.1.
CCDSiCCDS29516.1. [P49766-1]
CCDS50373.1. [P49766-2]
PIRiJC4679.
JC4680.
RefSeqiNP_001172093.1. NM_001185164.1. [P49766-2]
NP_035827.1. NM_011697.3. [P49766-1]
UniGeneiMm.15607.

3D structure databases

ProteinModelPortaliP49766.
SMRiP49766. Positions 34-128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP49766. 1 interaction.
MINTiMINT-1573475.

Proteomic databases

PRIDEiP49766.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025914; ENSMUSP00000025914; ENSMUSG00000024962. [P49766-1]
ENSMUST00000130048; ENSMUSP00000120860; ENSMUSG00000024962. [P49766-2]
GeneIDi22340.
KEGGimmu:22340.
UCSCiuc008gjv.2. mouse. [P49766-1]
uc008gjw.2. mouse. [P49766-2]

Organism-specific databases

CTDi7423.
MGIiMGI:106199. Vegfb.

Phylogenomic databases

eggNOGiNOG77417.
GeneTreeiENSGT00730000110791.
HOGENOMiHOG000230896.
HOVERGENiHBG000105.
InParanoidiP49766.
KOiK16858.
OMAiASTPHHR.
OrthoDBiEOG74R1S0.
PhylomeDBiP49766.
TreeFamiTF319554.

Enzyme and pathway databases

ReactomeiREACT_293283. VEGF binds to VEGFR leading to receptor dimerization.
REACT_307071. Platelet degranulation.
REACT_343634. VEGF ligand-receptor interactions.

Miscellaneous databases

ChiTaRSiVegfb. mouse.
NextBioi302601.
PROiP49766.
SOURCEiSearch...

Gene expression databases

BgeeiP49766.
CleanExiMM_VEGFB.
GenevestigatoriP49766.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
[Graphical view]
PfamiPF00341. PDGF. 1 hit.
[Graphical view]
SMARTiSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-B186 AND VEGF-B167).
    Tissue: Brain.
  2. "Genomic organization of the mouse and human genes for vascular endothelial growth factor B (VEGF-B) and characterization of a second splice isoform."
    Olofsson B., Pajusola K., von Euler G., Chilov D., Alitalo K., Eriksson U.
    J. Biol. Chem. 271:19310-19317(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-B186).
    Tissue: Heart.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-B167).
    Tissue: Heart.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM VEGF-B186).
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM VEGF-B167).
    Tissue: Eye.
  6. "Vascular endothelial growth factor-B-deficient mice display an atrial conduction defect."
    Aase K., von Euler G., Li X., Ponten A., Thoren P., Cao R., Cao Y., Olofsson B., Gebre-Medhin S., Pekny M., Alitalo K., Betsholtz C., Eriksson U.
    Circulation 104:358-364(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiVEGFB_MOUSE
AccessioniPrimary (citable) accession number: P49766
Secondary accession number(s): Q3UG04, Q5D0B1, Q64290
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1996
Last sequence update: November 15, 2001
Last modified: March 31, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.