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P49766 (VEGFB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vascular endothelial growth factor B

Short name=VEGF-B
Alternative name(s):
VEGF-related factor
Short name=VRF
Gene names
Name:Vegfb
Synonyms:Vrf
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length207 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. VEGF-B seems to be required for normal heart function in adult but is not required for proper development of the cardiovascular system either during development or for angiogenesis in adults. Ref.6

Subunit structure

Homodimer; disulfide-linked. Can also form heterodimer with VEGF.

Subcellular location

Secreted. Note: Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Tissue specificity

Abundantly expressed in heart, brain, kidney and skeletal muscle.

Post-translational modification

VEGF-B186 is O-glycosylated.

Sequence similarities

Belongs to the PDGF/VEGF growth factor family.

Ontologies

Keywords
   Biological processAngiogenesis
Differentiation
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   LigandHeparin-binding
   Molecular functionDevelopmental protein
Growth factor
Mitogen
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processangiogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

cardiac muscle contraction

Inferred from mutant phenotype PubMed 10666423. Source: MGI

cell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

coronary vasculature development

Inferred from mutant phenotype PubMed 10666423. Source: MGI

heart development

Inferred from mutant phenotype PubMed 10666423. Source: MGI

induction of positive chemotaxis

Inferred from electronic annotation. Source: Ensembl

negative regulation of gene expression

Inferred from electronic annotation. Source: Ensembl

negative regulation of neuron apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of ERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell division

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of endothelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of mast cell chemotaxis

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein kinase B signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of vascular endothelial growth factor receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of vascular wound healing

Inferred from electronic annotation. Source: Ensembl

protein O-linked glycosylation

Inferred from direct assay Ref.2. Source: MGI

response to drug

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentextracellular region

Inferred from direct assay Ref.2. Source: MGI

extracellular space

Inferred from electronic annotation. Source: Ensembl

intracellular

Inferred from direct assay Ref.2. Source: MGI

membrane

Inferred from electronic annotation. Source: InterPro

   Molecular_functionchemoattractant activity

Inferred from electronic annotation. Source: Ensembl

heparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein heterodimerization activity

Inferred from physical interaction Ref.2. Source: MGI

protein homodimerization activity

Inferred from physical interaction Ref.2. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform VEGF-B186 (identifier: P49766-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform VEGF-B167 (identifier: P49766-2)

The sequence of this isoform differs from the canonical sequence as follows:
     137-207: RVAIPHHRPQ...AASSIAKGGA → SPRILCPPCT...DTCRCRKPRK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 207186Vascular endothelial growth factor B
PRO_0000023399

Amino acid modifications

Disulfide bond47 ↔ 89 By similarity
Disulfide bond72Interchain By similarity
Disulfide bond78 ↔ 122 By similarity
Disulfide bond81Interchain By similarity
Disulfide bond82 ↔ 124 By similarity

Natural variations

Alternative sequence137 – 20771RVAIP…AKGGA → SPRILCPPCTQRRQRPDPRT CRCRCRRRRFLHCQGRGLEL NPDTCRCRKPRK in isoform VEGF-B167.
VSP_004641

Experimental info

Sequence conflict691V → A in BAE28405. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform VEGF-B186 [UniParc].

Last modified November 16, 2001. Version 2.
Checksum: 4817A5E96F6960C2

FASTA20721,914
        10         20         30         40         50         60 
MSPLLRRLLL VALLQLARTQ APVSQFDGPS HQKKVVPWID VYARATCQPR EVVVPLSMEL 

        70         80         90        100        110        120 
MGNVVKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIQYPSSQ LGEMSLEEHS 

       130        140        150        160        170        180 
QCECRPKKKE SAVKPDRVAI PHHRPQPRSV PGWDSTPGAS SPADIIHPTP APGSSARLAP 

       190        200 
SAVNALTPGP AAAAADAAAS SIAKGGA 

« Hide

Isoform VEGF-B167 [UniParc].

Checksum: D52A055FB995E9CA
Show »

FASTA18821,442

References

« Hide 'large scale' references
[1]"Characterization of the murine VEGF-related factor gene."
Townson S., Lagercrantz J., Grimmond S., Silins G., Nordenskjoeld M., Weber G., Hayward N.K.
Biochem. Biophys. Res. Commun. 220:922-928(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-B186 AND VEGF-B167).
Tissue: Brain.
[2]"Genomic organization of the mouse and human genes for vascular endothelial growth factor B (VEGF-B) and characterization of a second splice isoform."
Olofsson B., Pajusola K., von Euler G., Chilov D., Alitalo K., Eriksson U.
J. Biol. Chem. 271:19310-19317(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-B186).
Tissue: Heart.
[3]"Vascular endothelial growth factor B, a novel growth factor for endothelial cells."
Olofsson B., Pajusola K., Kaipainen A., von Euler G., Joukov V., Saksela O., Orpana A., Pettersson R.F., Alitalo K., Eriksson U.
Proc. Natl. Acad. Sci. U.S.A. 93:2576-2581(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-B167).
Tissue: Heart.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM VEGF-B186).
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM VEGF-B167).
Tissue: Eye.
[6]"Vascular endothelial growth factor-B-deficient mice display an atrial conduction defect."
Aase K., von Euler G., Li X., Ponten A., Thoren P., Cao R., Cao Y., Olofsson B., Gebre-Medhin S., Pekny M., Alitalo K., Betsholtz C., Eriksson U.
Circulation 104:358-364(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43836 mRNA. Translation: AAC52932.1.
U43837 mRNA. Translation: AAC52553.1.
U52820 mRNA. Translation: AAC52823.1.
U48800 mRNA. Translation: AAB06273.1.
AK148188 mRNA. Translation: BAE28405.1.
BC046303 mRNA. Translation: AAH46303.1.
CCDSCCDS29516.1. [P49766-1]
CCDS50373.1. [P49766-2]
PIRJC4679.
JC4680.
RefSeqNP_001172093.1. NM_001185164.1. [P49766-2]
NP_035827.1. NM_011697.3. [P49766-1]
UniGeneMm.15607.

3D structure databases

ProteinModelPortalP49766.
SMRP49766. Positions 34-128.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP49766. 1 interaction.
MINTMINT-1573475.

Proteomic databases

PRIDEP49766.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025914; ENSMUSP00000025914; ENSMUSG00000024962. [P49766-1]
ENSMUST00000130048; ENSMUSP00000120860; ENSMUSG00000024962. [P49766-2]
GeneID22340.
KEGGmmu:22340.
UCSCuc008gjv.2. mouse. [P49766-1]
uc008gjw.2. mouse. [P49766-2]

Organism-specific databases

CTD7423.
MGIMGI:106199. Vegfb.

Phylogenomic databases

eggNOGNOG77417.
GeneTreeENSGT00730000110791.
HOGENOMHOG000230896.
HOVERGENHBG000105.
InParanoidP49766.
KOK16858.
OMAASTPHHR.
OrthoDBEOG74R1S0.
PhylomeDBP49766.
TreeFamTF319554.

Gene expression databases

BgeeP49766.
CleanExMM_VEGFB.
GenevestigatorP49766.

Family and domain databases

Gene3D2.10.90.10. 1 hit.
InterProIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
[Graphical view]
PfamPF00341. PDGF. 1 hit.
[Graphical view]
SMARTSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMSSF57501. SSF57501. 1 hit.
PROSITEPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302601.
PROP49766.
SOURCESearch...

Entry information

Entry nameVEGFB_MOUSE
AccessionPrimary (citable) accession number: P49766
Secondary accession number(s): Q3UG04, Q5D0B1, Q64290
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 16, 2001
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot