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P49765 (VEGFB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vascular endothelial growth factor B

Short name=VEGF-B
Alternative name(s):
VEGF-related factor
Short name=VRF
Gene names
Name:VEGFB
Synonyms:VRF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length207 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

Subunit structure

Homodimer; disulfide-linked. Can also form heterodimer with VEGF. Ref.5

Subcellular location

Secreted. Note: Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Tissue specificity

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Post-translational modification

VEGF-B186 is O-glycosylated By similarity.

Sequence similarities

Belongs to the PDGF/VEGF growth factor family.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   LigandHeparin-binding
   Molecular functionGrowth factor
Mitogen
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processangiogenesis

Inferred from electronic annotation. Source: Ensembl

blood coagulation

Traceable author statement. Source: Reactome

cardiac muscle contraction

Inferred from electronic annotation. Source: Ensembl

coronary vasculature development

Inferred from electronic annotation. Source: Ensembl

induction of positive chemotaxis

Inferred from direct assay PubMed 19275959. Source: UniProtKB

negative regulation of apoptotic process

Inferred from direct assay PubMed 18259607. Source: UniProtKB

negative regulation of gene expression

Inferred from direct assay PubMed 18259607. Source: UniProtKB

negative regulation of neuron apoptotic process

Inferred from direct assay PubMed 18259607. Source: UniProtKB

platelet activation

Traceable author statement. Source: Reactome

platelet degranulation

Traceable author statement. Source: Reactome

positive chemotaxis

Inferred from direct assay PubMed 19275959. Source: GOC

positive regulation of ERK1 and ERK2 cascade

Inferred from direct assay PubMed 18259607. Source: UniProtKB

positive regulation of cell division

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of endothelial cell proliferation

Inferred from direct assay PubMed 11122379Ref.3. Source: UniProtKB

positive regulation of mast cell chemotaxis

Inferred from direct assay PubMed 19275959. Source: UniProtKB

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 11122379. Source: UniProtKB

positive regulation of protein kinase B signaling

Inferred from direct assay PubMed 18259607. Source: UniProtKB

positive regulation of vascular endothelial growth factor receptor signaling pathway

Inferred from direct assay PubMed 18259607. Source: UniProtKB

positive regulation of vascular wound healing

Inferred from direct assay PubMed 16109918PubMed 16109918. Source: UniProtKB

protein O-linked glycosylation

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

vascular endothelial growth factor receptor signaling pathway

Traceable author statement. Source: Reactome

   Cellular_componentextracellular region

Traceable author statement. Source: Reactome

extracellular space

Inferred from direct assay PubMed 12881472Ref.3. Source: UniProtKB

membrane

Inferred from electronic annotation. Source: InterPro

platelet alpha granule lumen

Traceable author statement. Source: Reactome

   Molecular_functionchemoattractant activity

Inferred from direct assay PubMed 19275959. Source: UniProtKB

heparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 19483306PubMed 11122379PubMed 19483306. Source: UniProtKB

vascular endothelial growth factor receptor 1 binding

Inferred from physical interaction PubMed 12366396PubMed 12366396PubMed 16109918. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform VEGF-B186 (identifier: P49765-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform VEGF-B167 (identifier: P49765-2)

The sequence of this isoform differs from the canonical sequence as follows:
     137-188: RAATPHHRPQ...APSTTSALTP → SPRPLCPRCT...DTCRCRKLRR
     189-207: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 207186Vascular endothelial growth factor B
PRO_0000023398

Amino acid modifications

Disulfide bond47 ↔ 89 Ref.5
Disulfide bond72Interchain Ref.5
Disulfide bond78 ↔ 122 Ref.5
Disulfide bond81Interchain Ref.5
Disulfide bond82 ↔ 124 Ref.5

Natural variations

Alternative sequence137 – 18852RAATP…SALTP → SPRPLCPRCTQHHQRPDPRT CRCRCRRRSFLRCQGRGLEL NPDTCRCRKLRR in isoform VEGF-B167.
VSP_004639
Alternative sequence189 – 20719Missing in isoform VEGF-B167.
VSP_004640

Secondary structure

................. 207
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform VEGF-B186 [UniParc].

Last modified November 16, 2001. Version 2.
Checksum: EDE4B1C0DFDAD6BC

FASTA20721,602
        10         20         30         40         50         60 
MSPLLRRLLL AALLQLAPAQ APVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL 

        70         80         90        100        110        120 
MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS 

       130        140        150        160        170        180 
QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP 

       190        200 
STTSALTPGP AAAAADAAAS SVAKGGA 

« Hide

Isoform VEGF-B167 [UniParc].

Checksum: F04654D5A3727194
Show »

FASTA18821,261

References

« Hide 'large scale' references
[1]"Cloning and characterization of a novel human gene related to vascular endothelial growth factor."
Grimmond S., Lagercrantz J., Drinkwater C., Silins G., Townson S., Pollock P., Gotley D., Carson E., Rakar S., Nordenskjoeld M., Ward L., Hayward N.K., Weber G.
Genome Res. 6:124-131(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-B186 AND VEGF-B167).
Tissue: Fetal brain.
[2]"Genomic organization of the mouse and human genes for vascular endothelial growth factor B (VEGF-B) and characterization of a second splice isoform."
Olofsson B., Pajusola K., von Euler G., Chilov D., Alitalo K., Eriksson U.
J. Biol. Chem. 271:19310-19317(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-B186).
Tissue: Fibrosarcoma.
[3]"Vascular endothelial growth factor B, a novel growth factor for endothelial cells."
Olofsson B., Pajusola K., Kaipainen A., von Euler G., Joukov V., Saksela O., Orpana A., Pettersson R.F., Alitalo K., Eriksson U.
Proc. Natl. Acad. Sci. U.S.A. 93:2576-2581(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-B167).
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM VEGF-B186).
Tissue: Tonsil.
[5]"Crystal structure of human vascular endothelial growth factor-B: identification of amino acids important for receptor binding."
Iyer S., Scotney P.D., Nash A.D., Ravi Acharya K.
J. Mol. Biol. 359:76-85(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.48 ANGSTROMS) OF 31-129, DISULFIDE BONDS, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43368 mRNA. Translation: AAA91462.1.
U43369 mRNA. Translation: AAA91463.1.
U52819 mRNA. Translation: AAC50721.1.
U48801 mRNA. Translation: AAB06274.1.
BC008818 mRNA. Translation: AAH08818.1.
CCDSCCDS58144.1. [P49765-2]
CCDS8062.1. [P49765-1]
RefSeqNP_001230662.1. NM_001243733.1. [P49765-2]
NP_003368.1. NM_003377.4. [P49765-1]
UniGeneHs.732095.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2C7WX-ray2.48A/B31-129[»]
2VWEX-ray3.40A/B22-196[»]
2XACX-ray2.71A/B31-129[»]
ProteinModelPortalP49765.
SMRP49765. Positions 31-129.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113266. 2 interactions.
DIPDIP-6045N.
IntActP49765. 1 interaction.
STRING9606.ENSP00000311127.

Polymorphism databases

DMDM17380554.

Proteomic databases

PaxDbP49765.
PRIDEP49765.

Protocols and materials databases

DNASU7423.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000309422; ENSP00000311127; ENSG00000173511. [P49765-1]
ENST00000426086; ENSP00000401550; ENSG00000173511. [P49765-2]
GeneID7423.
KEGGhsa:7423.
UCSCuc001nyw.3. human. [P49765-1]
uc001nyx.3. human. [P49765-2]

Organism-specific databases

CTD7423.
GeneCardsGC11P064002.
HGNCHGNC:12681. VEGFB.
HPACAB025499.
HPA044361.
MIM601398. gene.
neXtProtNX_P49765.
PharmGKBPA37303.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG77417.
HOGENOMHOG000230896.
HOVERGENHBG000105.
InParanoidP49765.
KOK16858.
OMAASTPHHR.
OrthoDBEOG74R1S0.
PhylomeDBP49765.
TreeFamTF319554.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_604. Hemostasis.
SignaLinkP49765.

Gene expression databases

ArrayExpressP49765.
BgeeP49765.
CleanExHS_VEGFB.
GenevestigatorP49765.

Family and domain databases

Gene3D2.10.90.10. 1 hit.
InterProIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
[Graphical view]
PfamPF00341. PDGF. 1 hit.
[Graphical view]
SMARTSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMSSF57501. SSF57501. 1 hit.
PROSITEPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP49765.
GeneWikiVascular_endothelial_growth_factor_B.
GenomeRNAi7423.
NextBio29076.
PROP49765.
SOURCESearch...

Entry information

Entry nameVEGFB_HUMAN
AccessionPrimary (citable) accession number: P49765
Secondary accession number(s): Q16528
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 16, 2001
Last modified: July 9, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM