Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P49762

- DOA_DROME

UniProt

P49762 - DOA_DROME

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Serine/threonine-protein kinase Doa

Gene

Doa

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Negative regulator of the copia retrotransposon element of the white (w) gene. In the eye, it is required for normal pigmentation, photoreceptor cell development and for organization of interommatidial bristles. Also essential for embryonic segmentation and differentiation of the nervous system. Functions in the control of alternative splicing by phosphorylating the arginine/serine-rich splicing factors, SR proteins.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei508 – 5081ATP
Active sitei605 – 6051Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi485 – 4939ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein kinase activity Source: FlyBase
  3. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-EC
  4. protein serine/threonine kinase activity Source: FlyBase
  5. protein tyrosine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. autophagic cell death Source: FlyBase
  2. blastoderm segmentation Source: FlyBase
  3. brain morphogenesis Source: FlyBase
  4. compound eye development Source: FlyBase
  5. compound eye photoreceptor development Source: FlyBase
  6. karyosome formation Source: FlyBase
  7. locomotion involved in locomotory behavior Source: FlyBase
  8. negative regulation of male germ cell proliferation Source: FlyBase
  9. nervous system development Source: FlyBase
  10. oogenesis Source: FlyBase
  11. protein autophosphorylation Source: FlyBase
  12. protein phosphorylation Source: FlyBase
  13. protein secretion Source: FlyBase
  14. regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
  15. salivary gland cell autophagic cell death Source: FlyBase
  16. sex differentiation Source: FlyBase
  17. startle response Source: FlyBase
  18. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 1994.
SignaLinkiP49762.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Doa (EC:2.7.12.1)
Alternative name(s):
Protein darkener of apricot
Gene namesi
Name:Doa
ORF Names:CG42320
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0265998. Doa.

Subcellular locationi

Isoform A : Nucleus
Note: Mainly nuclear with only low levels present in the cytoplasm.

GO - Cellular componenti

  1. cytoplasm Source: FlyBase
  2. endoplasmic reticulum Source: FlyBase
  3. microtubule associated complex Source: FlyBase
  4. nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi508 – 5081K → R: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 832832Serine/threonine-protein kinase DoaPRO_0000085926Add
BLAST

Post-translational modificationi

Autophosphorylated on serine, threonine and tyrosine residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49762.
PRIDEiP49762.

Expressioni

Tissue specificityi

Ubiquitous expression in embryos. Stage 17 embryos show elevated expression in CNS and brain. Ubiquitous expression in larval imaginal disks. Increased expression posterior to the eye-antennal disk morphogenetic furrow.2 Publications

Developmental stagei

Expressed both maternally (isoform C) and zygotically (isoforms A and C) in all developmental stages.2 Publications

Gene expression databases

BgeeiP49762.
ExpressionAtlasiP49762. differential.

Interactioni

Protein-protein interaction databases

BioGridi68286. 50 interactions.
IntActiP49762. 48 interactions.
MINTiMINT-805690.

Structurei

3D structure databases

ProteinModelPortaliP49762.
SMRiP49762. Positions 459-802.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini479 – 799321Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00580000081366.
HOGENOMiHOG000141611.
InParanoidiP49762.
KOiK08823.
OMAiDEDTICD.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform C (identifier: P49762-1) [UniParc]FASTAAdd to Basket

Also known as: 105 kDa isoform

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVAANLEVPT SSSSSAATKR QKDVDNKLEK CLNDMLKLKT SSNNNSTSNS
60 70 80 90 100
NNNAIMSHSL TGEHKDPKTA LEGPTSSSSS SSSKYIGESQ IPVPVQLYDP
110 120 130 140 150
QKPLLQQQQQ QQRICYPIGK SNSTSQLPMG GYQRLLQHQQ QQHHQQQQQQ
160 170 180 190 200
HQEQQQYPQH KRPFLNWNSF ACSAMNGASD PFMQQQHMPA HQQQQHLPHK
210 220 230 240 250
LQQSYSSSHV PKQAPKSGLA MFLQKNTNKE NKFGQPMQQQ PPGMMPQMYG
260 270 280 290 300
YQAPQQQSKI GYPRTGAPLT HSASFSSAQR PTALQFHQQH QQQQHLQQQQ
310 320 330 340 350
QHPQQQQHQH SSFGVGMMSR NYYNMPKQPE RKPLQTFDPY AYPKPNQMQP
360 370 380 390 400
VKYQQQQQHP HTQFQNASAG GGGGGAAGLQ YDPNTNTQLF YASPASSSSN
410 420 430 440 450
KQPQQPQQQQ QQQQSQLQQS NSVIFNHSGQ QHQPHQQQQN EMSKSALGLH
460 470 480 490 500
FIETAKPVIQ DDADGHLIYH TGDILHHRYK IMATLGEGTF GRVVKVKDME
510 520 530 540 550
RDYCMALKII KNVEKYREAA KLEINALEKI AQKDPHCDHL CVKMIDWFDY
560 570 580 590 600
HGHMCIVFEM LGLSVFDFLR ENNYEPYPLD QVRHMAYQLC YSVKFLHDNR
610 620 630 640 650
LTHTDLKPEN ILFVDSDYTS HYNHKINREV RRVKNTDVRL IDFGSATFDH
660 670 680 690 700
EHHSTIVSTR HYRAPEVILE LGWSQPCDVW SIGCILFELY LGITLFQTHD
710 720 730 740 750
NREHLAMMER ILGQIPYRMA RNHTLYSKTK TKYFYHGKLD WDEKSSAGRY
760 770 780 790 800
VRDHCKPLFL CQLSDSEDHC ELFSLIKKML EYEPSSRITL GEALHHPFFD
810 820 830
RLPPHHRVGE VSNKQPLSSG SSSRERSHSL SR

Note: No experimental confirmation available. May correspond to the described 105 kDa isoform although there is no translation sequence evidence for this.

Length:832
Mass (Da):95,723
Last modified:September 19, 2003 - v2
Checksum:i6EC90CB1A031DD68
GO
Isoform A (identifier: P49762-2) [UniParc]FASTAAdd to Basket

Also known as: 55 kDa isoform

The sequence of this isoform differs from the canonical sequence as follows:
     1-252: Missing.
     253-453: APQQQSKIGY...KSALGLHFIE → MQLPSLKDKL...GRNNRQSQAK
     722-727: Missing.

Show »
Length:580
Mass (Da):67,894
Checksum:iF73AC5B47733AF3A
GO
Isoform B (identifier: P49762-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-315: Missing.
     316-453: GMMSRNYYNM...KSALGLHFIE → MPRTRRLHHS...GRNNRQSQAK
     722-727: Missing.

Note: No experimental confirmation available.

Show »
Length:511
Mass (Da):60,210
Checksum:i8D6366BFDBD8A948
GO

Sequence cautioni

The sequence CAA55367.1 differs from that shown. Reason: Chimera. Chimera of genomic DNA and cDNA.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 315315Missing in isoform B. CuratedVSP_008269Add
BLAST
Alternative sequencei1 – 252252Missing in isoform A. 1 PublicationVSP_008268Add
BLAST
Alternative sequencei253 – 453201APQQQ…LHFIE → MQLPSLKDKLMPSGSVQQAK ANFSWQSLTQLLSGLWQRLY LPRSPFLALPAPPLATPPAN TTQRRAKKEMPRTRRLHHSR DRSSAGTRDKRRRHDTADHS PPLAEAPSPPRITNTHHTRS AAKRRRHELDAKKAQISKEP TFDDSISTRRRKERSKRSHR KSPAASRRQHKYRYRDETSH SSSRRRHRDRAKDERDSGRN NRQSQAK in isoform A. 1 PublicationVSP_008270Add
BLAST
Alternative sequencei316 – 453138GMMSR…LHFIE → MPRTRRLHHSRDRSSAGTRD KRRRHDTADHSPPLAEAPSP PRITNTHHTRSAAKRRRHEL DAKKAQISKEPTFDDSISTR RRKERSKRSHRKSPAASRRQ HKYRYRDETSHSSSRRRHRD RAKDERDSGRNNRQSQAK in isoform B. CuratedVSP_008271Add
BLAST
Alternative sequencei722 – 7276Missing in isoform A and isoform B. 1 PublicationVSP_008272

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78715 Genomic DNA. Translation: CAA55367.1. Sequence problems.
AE014297 Genomic DNA. Translation: AAF56832.3.
AE014297 Genomic DNA. Translation: AAF56833.2.
AE014297 Genomic DNA. Translation: AAN14305.1.
AY061474 mRNA. Translation: AAL29022.1.
AY069573 mRNA. Translation: AAL39718.1.
PIRiA54099.
RefSeqiNP_001014679.1. NM_001014679.2. [P49762-1]
NP_001014680.1. NM_001014680.4. [P49762-2]
NP_001014682.1. NM_001014682.3. [P49762-3]
NP_001036765.1. NM_001043300.3. [P49762-2]
NP_001138120.1. NM_001144648.3. [P49762-2]
NP_001138121.1. NM_001144649.2. [P49762-2]
NP_001138122.1. NM_001144650.1. [P49762-3]
UniGeneiDm.6767.

Genome annotation databases

EnsemblMetazoaiFBtr0299743; FBpp0289021; FBgn0259220. [P49762-1]
GeneIDi43415.
KEGGidme:Dmel_CG42320.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78715 Genomic DNA. Translation: CAA55367.1 . Sequence problems.
AE014297 Genomic DNA. Translation: AAF56832.3 .
AE014297 Genomic DNA. Translation: AAF56833.2 .
AE014297 Genomic DNA. Translation: AAN14305.1 .
AY061474 mRNA. Translation: AAL29022.1 .
AY069573 mRNA. Translation: AAL39718.1 .
PIRi A54099.
RefSeqi NP_001014679.1. NM_001014679.2. [P49762-1 ]
NP_001014680.1. NM_001014680.4. [P49762-2 ]
NP_001014682.1. NM_001014682.3. [P49762-3 ]
NP_001036765.1. NM_001043300.3. [P49762-2 ]
NP_001138120.1. NM_001144648.3. [P49762-2 ]
NP_001138121.1. NM_001144649.2. [P49762-2 ]
NP_001138122.1. NM_001144650.1. [P49762-3 ]
UniGenei Dm.6767.

3D structure databases

ProteinModelPortali P49762.
SMRi P49762. Positions 459-802.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 68286. 50 interactions.
IntActi P49762. 48 interactions.
MINTi MINT-805690.

Proteomic databases

PaxDbi P49762.
PRIDEi P49762.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0299743 ; FBpp0289021 ; FBgn0259220 . [P49762-1 ]
GeneIDi 43415.
KEGGi dme:Dmel_CG42320.

Organism-specific databases

CTDi 43415.
FlyBasei FBgn0265998. Doa.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00580000081366.
HOGENOMi HOG000141611.
InParanoidi P49762.
KOi K08823.
OMAi DEDTICD.

Enzyme and pathway databases

BRENDAi 2.7.11.1. 1994.
SignaLinki P49762.

Miscellaneous databases

ChiTaRSi Doa. fly.
GenomeRNAii 43415.
NextBioi 833791.
PROi P49762.

Gene expression databases

Bgeei P49762.
ExpressionAtlasi P49762. differential.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Doa locus encodes a member of a new protein kinase family and is essential for eye and embryonic development in Drosophila melanogaster."
    Yun B., Farkas R., Lee K., Rabinow L.
    Genes Dev. 8:1160-1173(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. Rabinow L.
    Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND C).
    Strain: Berkeley.
    Tissue: Embryo.
  6. "Activity and autophosphorylation of LAMMER protein kinases."
    Lee K., Du C., Horn M., Rabinow L.
    J. Biol. Chem. 271:27299-27303(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, AUTOPHOSPHORYLATION, MUTAGENESIS OF LYS-508.
  7. "The LAMMER protein kinase encoded by the Doa locus of Drosophila is required in both somatic and germline cells and is expressed as both nuclear and cytoplasmic isoforms throughout development."
    Yun B., Lee K., Farkas R., Hitte C., Rabinow L.
    Genetics 156:749-761(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiDOA_DROME
AccessioniPrimary (citable) accession number: P49762
Secondary accession number(s): Q8IMM0
, Q8T041, Q95RC9, Q9VAR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 19, 2003
Last modified: November 26, 2014
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3