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P49762 (DOA_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase Doa

EC=2.7.12.1
Alternative name(s):
Protein darkener of apricot
Gene names
Name:Doa
ORF Names:CG42320
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length832 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negative regulator of the copia retrotransposon element of the white (w) gene. In the eye, it is required for normal pigmentation, photoreceptor cell development and for organization of interommatidial bristles. Also essential for embryonic segmentation and differentiation of the nervous system. Functions in the control of alternative splicing by phosphorylating the arginine/serine-rich splicing factors, SR proteins. Ref.1 Ref.6 Ref.7

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium.

Subcellular location

Isoform C: Cytoplasmcytosol Ref.7.

Isoform A: Nucleus. Note: Mainly nuclear with only low levels present in the cytoplasm. Ref.7

Tissue specificity

Ubiquitous expression in embryos. Stage 17 embryos show elevated expression in CNS and brain. Ubiquitous expression in larval imaginal disks. Increased expression posterior to the eye-antennal disk morphogenetic furrow. Ref.1 Ref.7

Developmental stage

Expressed both maternally (isoform C)and zygotically (isoforms A and C) in all developmental stages. Ref.1 Ref.7

Post-translational modification

Autophosphorylated on serine, threonine and tyrosine residues. Ref.6

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence CAA55367.1 differs from that shown. Reason: Chimera. Chimera of genomic DNA and cDNA.

Ontologies

Keywords
   Biological processSensory transduction
Vision
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Kinase
Serine/threonine-protein kinase
Transferase
Tyrosine-protein kinase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processautophagic cell death

Inferred from expression pattern PubMed 12593804. Source: FlyBase

blastoderm segmentation

Inferred from mutant phenotype Ref.1. Source: FlyBase

brain morphogenesis

Inferred from mutant phenotype PubMed 18713854. Source: FlyBase

compound eye development

Inferred from mutant phenotype Ref.1. Source: FlyBase

compound eye photoreceptor development

Inferred from mutant phenotype Ref.7. Source: FlyBase

karyosome formation

Inferred from mutant phenotype PubMed 12930750. Source: FlyBase

locomotion involved in locomotory behavior

Inferred from mutant phenotype PubMed 18713854. Source: FlyBase

negative regulation of male germ cell proliferation

Inferred from mutant phenotype PubMed 23376537. Source: FlyBase

nervous system development

Inferred from mutant phenotype Ref.1. Source: FlyBase

oogenesis

Inferred from mutant phenotype PubMed 12930750. Source: FlyBase

protein autophosphorylation

Inferred from direct assay Ref.1. Source: FlyBase

protein phosphorylation

Non-traceable author statement PubMed 10908587. Source: FlyBase

protein secretion

Inferred from mutant phenotype PubMed 16452979. Source: FlyBase

regulation of alternative mRNA splicing, via spliceosome

Inferred from mutant phenotype PubMed 15492211. Source: FlyBase

salivary gland cell autophagic cell death

Inferred from expression pattern PubMed 12593804. Source: FlyBase

sex differentiation

Inferred from mutant phenotype PubMed 9885562. Source: FlyBase

startle response

Inferred from mutant phenotype PubMed 18713854. Source: FlyBase

visual perception

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from direct assay Ref.7PubMed 17828581. Source: FlyBase

cytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

endoplasmic reticulum

Inferred from direct assay PubMed 16452979. Source: FlyBase

microtubule associated complex

Inferred from direct assay PubMed 18433294. Source: FlyBase

nucleus

Inferred from direct assay Ref.7. Source: FlyBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein kinase activity

Inferred from direct assay PubMed 17828581PubMed 19841092Ref.1. Source: FlyBase

protein serine/threonine kinase activity

Inferred from direct assay PubMed 9885562. Source: FlyBase

protein serine/threonine/tyrosine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

protein tyrosine kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform C (identifier: P49762-1)

Also known as: 105 kDa isoform;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available. May correspond to the described 105 kDa isoform although there is no translation sequence evidence for this.
Isoform A (identifier: P49762-2)

Also known as: 55 kDa isoform;

The sequence of this isoform differs from the canonical sequence as follows:
     1-252: Missing.
     253-453: APQQQSKIGY...KSALGLHFIE → MQLPSLKDKL...GRNNRQSQAK
     722-727: Missing.
Isoform B (identifier: P49762-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-315: Missing.
     316-453: GMMSRNYYNM...KSALGLHFIE → MPRTRRLHHS...GRNNRQSQAK
     722-727: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 832832Serine/threonine-protein kinase Doa
PRO_0000085926

Regions

Domain479 – 799321Protein kinase
Nucleotide binding485 – 4939ATP By similarity

Sites

Active site6051Proton acceptor By similarity
Binding site5081ATP

Natural variations

Alternative sequence1 – 315315Missing in isoform B.
VSP_008269
Alternative sequence1 – 252252Missing in isoform A.
VSP_008268
Alternative sequence253 – 453201APQQQ…LHFIE → MQLPSLKDKLMPSGSVQQAK ANFSWQSLTQLLSGLWQRLY LPRSPFLALPAPPLATPPAN TTQRRAKKEMPRTRRLHHSR DRSSAGTRDKRRRHDTADHS PPLAEAPSPPRITNTHHTRS AAKRRRHELDAKKAQISKEP TFDDSISTRRRKERSKRSHR KSPAASRRQHKYRYRDETSH SSSRRRHRDRAKDERDSGRN NRQSQAK in isoform A.
VSP_008270
Alternative sequence316 – 453138GMMSR…LHFIE → MPRTRRLHHSRDRSSAGTRD KRRRHDTADHSPPLAEAPSP PRITNTHHTRSAAKRRRHEL DAKKAQISKEPTFDDSISTR RRKERSKRSHRKSPAASRRQ HKYRYRDETSHSSSRRRHRD RAKDERDSGRNNRQSQAK in isoform B.
VSP_008271
Alternative sequence722 – 7276Missing in isoform A and isoform B.
VSP_008272

Experimental info

Mutagenesis5081K → R: Loss of activity. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform C (105 kDa isoform) [UniParc].

Last modified September 19, 2003. Version 2.
Checksum: 6EC90CB1A031DD68

FASTA83295,723
        10         20         30         40         50         60 
MVAANLEVPT SSSSSAATKR QKDVDNKLEK CLNDMLKLKT SSNNNSTSNS NNNAIMSHSL 

        70         80         90        100        110        120 
TGEHKDPKTA LEGPTSSSSS SSSKYIGESQ IPVPVQLYDP QKPLLQQQQQ QQRICYPIGK 

       130        140        150        160        170        180 
SNSTSQLPMG GYQRLLQHQQ QQHHQQQQQQ HQEQQQYPQH KRPFLNWNSF ACSAMNGASD 

       190        200        210        220        230        240 
PFMQQQHMPA HQQQQHLPHK LQQSYSSSHV PKQAPKSGLA MFLQKNTNKE NKFGQPMQQQ 

       250        260        270        280        290        300 
PPGMMPQMYG YQAPQQQSKI GYPRTGAPLT HSASFSSAQR PTALQFHQQH QQQQHLQQQQ 

       310        320        330        340        350        360 
QHPQQQQHQH SSFGVGMMSR NYYNMPKQPE RKPLQTFDPY AYPKPNQMQP VKYQQQQQHP 

       370        380        390        400        410        420 
HTQFQNASAG GGGGGAAGLQ YDPNTNTQLF YASPASSSSN KQPQQPQQQQ QQQQSQLQQS 

       430        440        450        460        470        480 
NSVIFNHSGQ QHQPHQQQQN EMSKSALGLH FIETAKPVIQ DDADGHLIYH TGDILHHRYK 

       490        500        510        520        530        540 
IMATLGEGTF GRVVKVKDME RDYCMALKII KNVEKYREAA KLEINALEKI AQKDPHCDHL 

       550        560        570        580        590        600 
CVKMIDWFDY HGHMCIVFEM LGLSVFDFLR ENNYEPYPLD QVRHMAYQLC YSVKFLHDNR 

       610        620        630        640        650        660 
LTHTDLKPEN ILFVDSDYTS HYNHKINREV RRVKNTDVRL IDFGSATFDH EHHSTIVSTR 

       670        680        690        700        710        720 
HYRAPEVILE LGWSQPCDVW SIGCILFELY LGITLFQTHD NREHLAMMER ILGQIPYRMA 

       730        740        750        760        770        780 
RNHTLYSKTK TKYFYHGKLD WDEKSSAGRY VRDHCKPLFL CQLSDSEDHC ELFSLIKKML 

       790        800        810        820        830 
EYEPSSRITL GEALHHPFFD RLPPHHRVGE VSNKQPLSSG SSSRERSHSL SR 

« Hide

Isoform A (55 kDa isoform) [UniParc].

Checksum: F73AC5B47733AF3A
Show »

FASTA58067,894
Isoform B [UniParc].

Checksum: 8D6366BFDBD8A948
Show »

FASTA51160,210

References

« Hide 'large scale' references
[1]"The Doa locus encodes a member of a new protein kinase family and is essential for eye and embryonic development in Drosophila melanogaster."
Yun B., Farkas R., Lee K., Rabinow L.
Genes Dev. 8:1160-1173(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]Rabinow L.
Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[4]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[5]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND C).
Strain: Berkeley.
Tissue: Embryo.
[6]"Activity and autophosphorylation of LAMMER protein kinases."
Lee K., Du C., Horn M., Rabinow L.
J. Biol. Chem. 271:27299-27303(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, AUTOPHOSPHORYLATION, MUTAGENESIS OF LYS-508.
[7]"The LAMMER protein kinase encoded by the Doa locus of Drosophila is required in both somatic and germline cells and is expressed as both nuclear and cytoplasmic isoforms throughout development."
Yun B., Lee K., Farkas R., Hitte C., Rabinow L.
Genetics 156:749-761(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78715 Genomic DNA. Translation: CAA55367.1. Sequence problems.
AE014297 Genomic DNA. Translation: AAF56832.3.
AE014297 Genomic DNA. Translation: AAF56833.2.
AE014297 Genomic DNA. Translation: AAN14305.1.
AY061474 mRNA. Translation: AAL29022.1.
AY069573 mRNA. Translation: AAL39718.1.
PIRA54099.
RefSeqNP_001014679.1. NM_001014679.2. [P49762-1]
NP_001014680.1. NM_001014680.4. [P49762-2]
NP_001014682.1. NM_001014682.3. [P49762-3]
NP_001036765.1. NM_001043300.3. [P49762-2]
NP_001138120.1. NM_001144648.3. [P49762-2]
NP_001138121.1. NM_001144649.2. [P49762-2]
NP_001138122.1. NM_001144650.1. [P49762-3]
UniGeneDm.6767.

3D structure databases

ProteinModelPortalP49762.
SMRP49762. Positions 459-802.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid68286. 50 interactions.
IntActP49762. 48 interactions.
MINTMINT-805690.

Proteomic databases

PaxDbP49762.
PRIDEP49762.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0299743; FBpp0289021; FBgn0259220. [P49762-1]
GeneID43415.
KEGGdme:Dmel_CG42320.

Organism-specific databases

CTD43415.
FlyBaseFBgn0265998. Doa.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00580000081366.
HOGENOMHOG000141611.
KOK08823.
OMADEDTICD.

Enzyme and pathway databases

BRENDA2.7.11.1. 1994.
SignaLinkP49762.

Gene expression databases

BgeeP49762.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDoa. drosophila.
GenomeRNAi43415.
NextBio833791.
PROP49762.

Entry information

Entry nameDOA_DROME
AccessionPrimary (citable) accession number: P49762
Secondary accession number(s): Q8IMM0 expand/collapse secondary AC list , Q8T041, Q95RC9, Q9VAR8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 19, 2003
Last modified: July 9, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase