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Protein

Serine/threonine-protein kinase Doa

Gene

Doa

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of the copia retrotransposon element of the white (w) gene. In the eye, it is required for normal pigmentation, photoreceptor cell development and for organization of interommatidial bristles. Also essential for embryonic segmentation and differentiation of the nervous system. Functions in the control of alternative splicing by phosphorylating the arginine/serine-rich splicing factors, SR proteins.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei508ATP1
Active sitei605Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi485 – 493ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • blastoderm segmentation Source: FlyBase
  • compound eye development Source: FlyBase
  • compound eye photoreceptor development Source: FlyBase
  • karyosome formation Source: FlyBase
  • negative regulation of male germ cell proliferation Source: FlyBase
  • negative regulation of MyD88-dependent toll-like receptor signaling pathway Source: FlyBase
  • nervous system development Source: FlyBase
  • oogenesis Source: FlyBase
  • protein autophosphorylation Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • protein secretion Source: FlyBase
  • regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
  • response to stimulus Source: UniProtKB-KW
  • sex differentiation Source: FlyBase
  • visual perception Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase
Biological processSensory transduction, Vision
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 1994.
2.7.12.1. 1994.
SignaLinkiP49762.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Doa (EC:2.7.12.1)
Alternative name(s):
Protein darkener of apricot
Gene namesi
Name:Doa
ORF Names:CG42320
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0265998. Doa.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi508K → R: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859261 – 832Serine/threonine-protein kinase DoaAdd BLAST832

Post-translational modificationi

Autophosphorylated on serine, threonine and tyrosine residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49762.
PRIDEiP49762.

Expressioni

Tissue specificityi

Ubiquitous expression in embryos. Stage 17 embryos show elevated expression in CNS and brain. Ubiquitous expression in larval imaginal disks. Increased expression posterior to the eye-antennal disk morphogenetic furrow.2 Publications

Developmental stagei

Expressed both maternally (isoform C) and zygotically (isoforms A and C) in all developmental stages.2 Publications

Gene expression databases

BgeeiFBgn0265998.
ExpressionAtlasiP49762. differential.
GenevisibleiP49762. DM.

Interactioni

Protein-protein interaction databases

BioGridi68286. 55 interactors.
IntActiP49762. 50 interactors.
MINTiMINT-805690.
STRINGi7227.FBpp0289029.

Structurei

3D structure databases

ProteinModelPortaliP49762.
SMRiP49762.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini479 – 799Protein kinasePROSITE-ProRule annotationAdd BLAST321

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0671. Eukaryota.
ENOG410XQF2. LUCA.
GeneTreeiENSGT00580000081366.
HOGENOMiHOG000141611.
InParanoidiP49762.
KOiK08823.
OrthoDBiEOG091G033A.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom_sf.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform C (identifier: P49762-1) [UniParc]FASTAAdd to basket
Also known as: 105 kDa isoform

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVAANLEVPT SSSSSAATKR QKDVDNKLEK CLNDMLKLKT SSNNNSTSNS
60 70 80 90 100
NNNAIMSHSL TGEHKDPKTA LEGPTSSSSS SSSKYIGESQ IPVPVQLYDP
110 120 130 140 150
QKPLLQQQQQ QQRICYPIGK SNSTSQLPMG GYQRLLQHQQ QQHHQQQQQQ
160 170 180 190 200
HQEQQQYPQH KRPFLNWNSF ACSAMNGASD PFMQQQHMPA HQQQQHLPHK
210 220 230 240 250
LQQSYSSSHV PKQAPKSGLA MFLQKNTNKE NKFGQPMQQQ PPGMMPQMYG
260 270 280 290 300
YQAPQQQSKI GYPRTGAPLT HSASFSSAQR PTALQFHQQH QQQQHLQQQQ
310 320 330 340 350
QHPQQQQHQH SSFGVGMMSR NYYNMPKQPE RKPLQTFDPY AYPKPNQMQP
360 370 380 390 400
VKYQQQQQHP HTQFQNASAG GGGGGAAGLQ YDPNTNTQLF YASPASSSSN
410 420 430 440 450
KQPQQPQQQQ QQQQSQLQQS NSVIFNHSGQ QHQPHQQQQN EMSKSALGLH
460 470 480 490 500
FIETAKPVIQ DDADGHLIYH TGDILHHRYK IMATLGEGTF GRVVKVKDME
510 520 530 540 550
RDYCMALKII KNVEKYREAA KLEINALEKI AQKDPHCDHL CVKMIDWFDY
560 570 580 590 600
HGHMCIVFEM LGLSVFDFLR ENNYEPYPLD QVRHMAYQLC YSVKFLHDNR
610 620 630 640 650
LTHTDLKPEN ILFVDSDYTS HYNHKINREV RRVKNTDVRL IDFGSATFDH
660 670 680 690 700
EHHSTIVSTR HYRAPEVILE LGWSQPCDVW SIGCILFELY LGITLFQTHD
710 720 730 740 750
NREHLAMMER ILGQIPYRMA RNHTLYSKTK TKYFYHGKLD WDEKSSAGRY
760 770 780 790 800
VRDHCKPLFL CQLSDSEDHC ELFSLIKKML EYEPSSRITL GEALHHPFFD
810 820 830
RLPPHHRVGE VSNKQPLSSG SSSRERSHSL SR
Note: No experimental confirmation available. May correspond to the described 105 kDa isoform although there is no translation sequence evidence for this.
Length:832
Mass (Da):95,723
Last modified:September 19, 2003 - v2
Checksum:i6EC90CB1A031DD68
GO
Isoform A (identifier: P49762-2) [UniParc]FASTAAdd to basket
Also known as: 55 kDa isoform

The sequence of this isoform differs from the canonical sequence as follows:
     1-252: Missing.
     253-453: APQQQSKIGY...KSALGLHFIE → MQLPSLKDKL...GRNNRQSQAK
     722-727: Missing.

Show »
Length:580
Mass (Da):67,894
Checksum:iF73AC5B47733AF3A
GO
Isoform B (identifier: P49762-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-315: Missing.
     316-453: GMMSRNYYNM...KSALGLHFIE → MPRTRRLHHS...GRNNRQSQAK
     722-727: Missing.

Note: No experimental confirmation available.
Show »
Length:511
Mass (Da):60,210
Checksum:i8D6366BFDBD8A948
GO

Sequence cautioni

The sequence CAA55367 differs from that shown. Chimera. Chimera of genomic DNA and cDNA.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0082691 – 315Missing in isoform B. CuratedAdd BLAST315
Alternative sequenceiVSP_0082681 – 252Missing in isoform A. 1 PublicationAdd BLAST252
Alternative sequenceiVSP_008270253 – 453APQQQ…LHFIE → MQLPSLKDKLMPSGSVQQAK ANFSWQSLTQLLSGLWQRLY LPRSPFLALPAPPLATPPAN TTQRRAKKEMPRTRRLHHSR DRSSAGTRDKRRRHDTADHS PPLAEAPSPPRITNTHHTRS AAKRRRHELDAKKAQISKEP TFDDSISTRRRKERSKRSHR KSPAASRRQHKYRYRDETSH SSSRRRHRDRAKDERDSGRN NRQSQAK in isoform A. 1 PublicationAdd BLAST201
Alternative sequenceiVSP_008271316 – 453GMMSR…LHFIE → MPRTRRLHHSRDRSSAGTRD KRRRHDTADHSPPLAEAPSP PRITNTHHTRSAAKRRRHEL DAKKAQISKEPTFDDSISTR RRKERSKRSHRKSPAASRRQ HKYRYRDETSHSSSRRRHRD RAKDERDSGRNNRQSQAK in isoform B. CuratedAdd BLAST138
Alternative sequenceiVSP_008272722 – 727Missing in isoform A and isoform B. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78715 Genomic DNA. Translation: CAA55367.1. Sequence problems.
AE014297 Genomic DNA. Translation: AAF56832.3.
AE014297 Genomic DNA. Translation: AAF56833.2.
AE014297 Genomic DNA. Translation: AAN14305.1.
AY061474 mRNA. Translation: AAL29022.1.
AY069573 mRNA. Translation: AAL39718.1.
PIRiA54099.
RefSeqiNP_001014679.1. NM_001014679.2. [P49762-1]
NP_001014680.1. NM_001014680.4. [P49762-2]
NP_001014682.1. NM_001014682.3. [P49762-3]
NP_001036765.1. NM_001043300.3. [P49762-2]
NP_001138120.1. NM_001144648.3. [P49762-2]
NP_001138121.1. NM_001144649.2. [P49762-2]
NP_001138122.1. NM_001144650.1. [P49762-3]
UniGeneiDm.6767.

Genome annotation databases

EnsemblMetazoaiFBtr0299742; FBpp0289020; FBgn0265998. [P49762-2]
FBtr0299743; FBpp0289021; FBgn0265998. [P49762-1]
FBtr0299745; FBpp0289023; FBgn0265998. [P49762-2]
FBtr0299748; FBpp0289026; FBgn0265998. [P49762-3]
FBtr0299749; FBpp0289027; FBgn0265998. [P49762-2]
FBtr0299750; FBpp0289028; FBgn0265998. [P49762-2]
FBtr0299751; FBpp0289029; FBgn0265998.
FBtr0299752; FBpp0289030; FBgn0265998. [P49762-3]
GeneIDi43415.
KEGGidme:Dmel_CG42320.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDOA_DROME
AccessioniPrimary (citable) accession number: P49762
Secondary accession number(s): Q8IMM0
, Q8T041, Q95RC9, Q9VAR8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 19, 2003
Last modified: November 22, 2017
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families