Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thrombospondin-3

Gene

THBS3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen.

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • heparin binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciZFISH:ENSG00000169231-MONOMER.
ReactomeiR-HSA-186797. Signaling by PDGF.

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-3
Gene namesi
Name:THBS3
Synonyms:TSP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:11787. THBS3.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: Reactome
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi7059.
OpenTargetsiENSG00000169231.
PharmGKBiPA36499.

Polymorphism and mutation databases

BioMutaiTHBS3.
DMDMi1717814.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000003584923 – 956Thrombospondin-3Add BLAST934

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi266InterchainCurated
Disulfide bondi269InterchainCurated
Disulfide bondi278 ↔ 289PROSITE-ProRule annotation
Disulfide bondi283 ↔ 300PROSITE-ProRule annotation
Disulfide bondi303 ↔ 314PROSITE-ProRule annotation
Glycosylationi310N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi320 ↔ 332PROSITE-ProRule annotation
Disulfide bondi326 ↔ 341PROSITE-ProRule annotation
Disulfide bondi344 ↔ 368PROSITE-ProRule annotation
Disulfide bondi374 ↔ 388PROSITE-ProRule annotation
Disulfide bondi382 ↔ 397PROSITE-ProRule annotation
Disulfide bondi400 ↔ 412PROSITE-ProRule annotation
Glycosylationi407N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi418 ↔ 432PROSITE-ProRule annotation
Disulfide bondi426 ↔ 442PROSITE-ProRule annotation
Disulfide bondi444 ↔ 455PROSITE-ProRule annotation
Disulfide bondi471 ↔ 478PROSITE-ProRule annotation
Disulfide bondi483 ↔ 503PROSITE-ProRule annotation
Disulfide bondi519 ↔ 539PROSITE-ProRule annotation
Disulfide bondi542 ↔ 562PROSITE-ProRule annotation
Disulfide bondi578 ↔ 598PROSITE-ProRule annotation
Disulfide bondi601 ↔ 621PROSITE-ProRule annotation
Disulfide bondi639 ↔ 659PROSITE-ProRule annotation
Glycosylationi644N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi679 ↔ 699PROSITE-ProRule annotation
Disulfide bondi715 ↔ 936PROSITE-ProRule annotation
Glycosylationi937N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP49746.
PaxDbiP49746.
PeptideAtlasiP49746.
PRIDEiP49746.

PTM databases

iPTMnetiP49746.
PhosphoSitePlusiP49746.

Expressioni

Gene expression databases

BgeeiENSG00000169231.
CleanExiHS_THBS3.
ExpressionAtlasiP49746. baseline and differential.
GenevisibleiP49746. HS.

Organism-specific databases

HPAiHPA006293.

Interactioni

Subunit structurei

Oligomer; disulfide-linked.

Protein-protein interaction databases

BioGridi112917. 69 interactors.
STRINGi9606.ENSP00000357362.

Structurei

3D structure databases

ProteinModelPortaliP49746.
SMRiP49746.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 193Laminin G-likeAdd BLAST171
Domaini316 – 354EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd BLAST39
Domaini370 – 410EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini414 – 456EGF-like 3PROSITE-ProRule annotationAdd BLAST43
Repeati457 – 491TSP type-3 1Add BLAST35
Repeati492 – 527TSP type-3 2Add BLAST36
Repeati528 – 550TSP type-3 3Add BLAST23
Repeati551 – 586TSP type-3 4Add BLAST36
Repeati587 – 609TSP type-3 5Add BLAST23
Repeati610 – 647TSP type-3 6Add BLAST38
Repeati648 – 687TSP type-3 7Add BLAST40
Repeati688 – 723TSP type-3 8Add BLAST36
Domaini727 – 941TSP C-terminalPROSITE-ProRule annotationAdd BLAST215

Sequence similaritiesi

Belongs to the thrombospondin family.Curated
Contains 3 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.Curated
Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFQQ. Eukaryota.
ENOG410XQKE. LUCA.
GeneTreeiENSGT00850000132273.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiP49746.
KOiK04659.
OMAiYQDSGRF.
OrthoDBiEOG091G00TV.
PhylomeDBiP49746.
TreeFamiTF324917.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR028507. Thrombospondin-3.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PANTHERiPTHR10199:SF89. PTHR10199:SF89. 1 hit.
PfamiPF11598. COMP. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 4 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 3 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P49746-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METQELRGAL ALLLLCFFTS ASQDLQVIDL LTVGESRQMV AVAEKIRTAL
60 70 80 90 100
LTAGDIYLLS TFRLPPKQGG VLFGLYSRQD NTRWLEASVV GKINKVLVRY
110 120 130 140 150
QREDGKVHAV NLQQAGLADG RTHTVLLRLR GPSRPSPALH LYVDCKLGDQ
160 170 180 190 200
HAGLPALAPI PPAEVDGLEI RTGQKAYLRM QGFVESMKII LGGSMARVGA
210 220 230 240 250
LSECPFQGDE SIHSAVTNAL HSILGEQTKA LVTQLTLFNQ ILVELRDDIR
260 270 280 290 300
DQVKEMSLIR NTIMECQVCG FHEQRSHCSP NPCFRGVDCM EVYEYPGYRC
310 320 330 340 350
GPCPPGLQGN GTHCSDINEC AHADPCFPGS SCINTMPGFH CEACPRGYKG
360 370 380 390 400
TQVSGVGIDY ARASKQVCND IDECNDGNNG GCDPNSICTN TVGSFKCGPC
410 420 430 440 450
RLGFLGNQSQ GCLPARTCHS PAHSPCHIHA HCLFERNGAV SCQCNVGWAG
460 470 480 490 500
NGNVCGTDTD IDGYPDQALP CMDNNKHCKQ DNCLLTPNSG QEDADNDGVG
510 520 530 540 550
DQCDDDADGD GIKNVEDNCR LFPNKDQQNS DTDSFGDACD NCPNVPNNDQ
560 570 580 590 600
KDTDGNGEGD ACDNDVDGDG IPNGLDNCPK VPNPLQTDRD EDGVGDACDS
610 620 630 640 650
CPEMSNPTQT DADSDLVGDV CDTNEDSDGD GHQDTKDNCP QLPNSSQLDS
660 670 680 690 700
DNDGLGDECD GDDDNDGIPD YVPPGPDNCR LVPNPNQKDS DGNGVGDVCE
710 720 730 740 750
DDFDNDAVVD PLDVCPESAE VTLTDFRAYQ TVVLDPEGDA QIDPNWVVLN
760 770 780 790 800
QGMEIVQTMN SDPGLAVGYT AFNGVDFEGT FHVNTVTDDD YAGFLFSYQD
810 820 830 840 850
SGRFYVVMWK QTEQTYWQAT PFRAVAQPGL QLKAVTSVSG PGEHLRNALW
860 870 880 890 900
HTGHTPDQVR LLWTDPRNVG WRDKTSYRWQ LLHRPQVGYI RVKLYEGPQL
910 920 930 940 950
VADSGVIIDT SMRGGRLGVF CFSQENIIWS NLQYRCNDTV PEDFEPFRRQ

LLQGRV
Length:956
Mass (Da):104,201
Last modified:October 1, 1996 - v1
Checksum:iAE9B136DF0FFE5B8
GO
Isoform 2 (identifier: P49746-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     96-215: Missing.

Show »
Length:836
Mass (Da):91,297
Checksum:i8D7AA90E4B21CA96
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052658279S → G.Corresponds to variant rs35154152dbSNPEnsembl.1
Natural variantiVAR_035808955R → G in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04532896 – 215Missing in isoform 2. 1 PublicationAdd BLAST120

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38969 mRNA. Translation: AAC41762.1.
AL713999 Genomic DNA. Translation: CAI95084.1.
AF023268 Genomic DNA. Translation: AAC51818.1.
AK298592 mRNA. Translation: BAG60782.1.
CH471121 Genomic DNA. Translation: EAW53110.1.
BC018786 mRNA. Translation: AAH18786.1.
CCDSiCCDS1099.1. [P49746-1]
CCDS58034.1. [P49746-2]
PIRiA57121.
RefSeqiNP_001239536.1. NM_001252607.1.
NP_001239537.1. NM_001252608.1. [P49746-2]
NP_009043.1. NM_007112.4. [P49746-1]
UniGeneiHs.169875.
Hs.658188.

Genome annotation databases

EnsembliENST00000368378; ENSP00000357362; ENSG00000169231. [P49746-1]
ENST00000457183; ENSP00000392207; ENSG00000169231. [P49746-2]
GeneIDi7059.
KEGGihsa:7059.
UCSCiuc001fix.4. human. [P49746-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38969 mRNA. Translation: AAC41762.1.
AL713999 Genomic DNA. Translation: CAI95084.1.
AF023268 Genomic DNA. Translation: AAC51818.1.
AK298592 mRNA. Translation: BAG60782.1.
CH471121 Genomic DNA. Translation: EAW53110.1.
BC018786 mRNA. Translation: AAH18786.1.
CCDSiCCDS1099.1. [P49746-1]
CCDS58034.1. [P49746-2]
PIRiA57121.
RefSeqiNP_001239536.1. NM_001252607.1.
NP_001239537.1. NM_001252608.1. [P49746-2]
NP_009043.1. NM_007112.4. [P49746-1]
UniGeneiHs.169875.
Hs.658188.

3D structure databases

ProteinModelPortaliP49746.
SMRiP49746.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112917. 69 interactors.
STRINGi9606.ENSP00000357362.

PTM databases

iPTMnetiP49746.
PhosphoSitePlusiP49746.

Polymorphism and mutation databases

BioMutaiTHBS3.
DMDMi1717814.

Proteomic databases

EPDiP49746.
PaxDbiP49746.
PeptideAtlasiP49746.
PRIDEiP49746.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368378; ENSP00000357362; ENSG00000169231. [P49746-1]
ENST00000457183; ENSP00000392207; ENSG00000169231. [P49746-2]
GeneIDi7059.
KEGGihsa:7059.
UCSCiuc001fix.4. human. [P49746-1]

Organism-specific databases

CTDi7059.
DisGeNETi7059.
GeneCardsiTHBS3.
H-InvDBHIX0029532.
HGNCiHGNC:11787. THBS3.
HPAiHPA006293.
MIMi188062. gene.
neXtProtiNX_P49746.
OpenTargetsiENSG00000169231.
PharmGKBiPA36499.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFQQ. Eukaryota.
ENOG410XQKE. LUCA.
GeneTreeiENSGT00850000132273.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiP49746.
KOiK04659.
OMAiYQDSGRF.
OrthoDBiEOG091G00TV.
PhylomeDBiP49746.
TreeFamiTF324917.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000169231-MONOMER.
ReactomeiR-HSA-186797. Signaling by PDGF.

Miscellaneous databases

ChiTaRSiTHBS3. human.
GeneWikiiTHBS3.
GenomeRNAii7059.
PROiP49746.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169231.
CleanExiHS_THBS3.
ExpressionAtlasiP49746. baseline and differential.
GenevisibleiP49746. HS.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR028507. Thrombospondin-3.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PANTHERiPTHR10199:SF89. PTHR10199:SF89. 1 hit.
PfamiPF11598. COMP. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 4 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 3 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSP3_HUMAN
AccessioniPrimary (citable) accession number: P49746
Secondary accession number(s): B1AVR8, B4DQ20, Q8WV34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.