P49746 (TSP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thrombospondin-3

Gene names

Name:	THBS3
Synonyms:	TSP3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	956 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen.
Subunit structure	Oligomer; disulfide-linked.
Sequence similarities	Belongs to the thrombospondin family. Contains 3 EGF-like domains. Contains 1 laminin G-like domain. Contains 1 TSP C-terminal (TSPC) domain. Contains 8 TSP type-3 repeats.

Ontologies

Keywords
Biological process	Cell adhesion
Coding sequence diversity	Alternative splicing Polymorphism
Domain	EGF-like domain Repeat Signal
Ligand	Calcium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bone trabecula formation Inferred from electronic annotation. Source: Compara cell-matrix adhesion Non-traceable author statement Ref.1. Source: UniProtKB growth plate cartilage development Inferred from electronic annotation. Source: Compara ossification involved in bone maturation Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Traceable author statement. Source: Reactome perinuclear region of cytoplasm Inferred from direct assay PubMed 11943589. Source: UniProtKB
Molecular_function	calcium ion binding Non-traceable author statement Ref.1. Source: UniProtKB heparin binding Inferred from direct assay PubMed 8288588. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P49746-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P49746-2)

The sequence of this isoform differs from the canonical sequence as follows:
     96-215: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 956	934	Thrombospondin-3		PRO_0000035849
Regions
	Domain	23 – 193	171	Laminin G-like
	Domain	316 – 354	39	EGF-like 1; calcium-binding Potential
	Domain	370 – 410	41	EGF-like 2; calcium-binding Potential
	Domain	414 – 456	43	EGF-like 3
	Repeat	457 – 491	35	TSP type-3 1
	Repeat	492 – 527	36	TSP type-3 2
	Repeat	528 – 550	23	TSP type-3 3
	Repeat	551 – 586	36	TSP type-3 4
	Repeat	587 – 609	23	TSP type-3 5
	Repeat	610 – 647	38	TSP type-3 6
	Repeat	648 – 687	40	TSP type-3 7
	Repeat	688 – 723	36	TSP type-3 8
	Domain	727 – 941	215	TSP C-terminal
Amino acid modifications
	Glycosylation	310	1	N-linked (GlcNAc...) Potential
	Glycosylation	407	1	N-linked (GlcNAc...) Potential
	Glycosylation	644	1	N-linked (GlcNAc...) Potential
	Glycosylation	937	1	N-linked (GlcNAc...) Potential
	Disulfide bond	266		Interchain Probable
	Disulfide bond	269		Interchain Probable
	Disulfide bond	278 ↔ 289		By similarity
	Disulfide bond	283 ↔ 300		By similarity
	Disulfide bond	303 ↔ 314		By similarity
	Disulfide bond	320 ↔ 332		By similarity
	Disulfide bond	326 ↔ 341		By similarity
	Disulfide bond	344 ↔ 368		By similarity
	Disulfide bond	374 ↔ 388		By similarity
	Disulfide bond	382 ↔ 397		By similarity
	Disulfide bond	400 ↔ 412		By similarity
	Disulfide bond	418 ↔ 432		By similarity
	Disulfide bond	426 ↔ 442		By similarity
	Disulfide bond	444 ↔ 455		By similarity
	Disulfide bond	471 ↔ 478		By similarity
	Disulfide bond	483 ↔ 503		By similarity
	Disulfide bond	519 ↔ 539		By similarity
	Disulfide bond	542 ↔ 562		By similarity
	Disulfide bond	578 ↔ 598		By similarity
	Disulfide bond	601 ↔ 621		By similarity
	Disulfide bond	639 ↔ 659		By similarity
	Disulfide bond	679 ↔ 699		By similarity
	Disulfide bond	715 ↔ 936		By similarity
Natural variations
	Alternative sequence	96 – 215	120	Missing in isoform 2.		VSP_045328
	Natural variant	279	1	S → G. Corresponds to variant rs35154152 [ dbSNP | Ensembl ].		VAR_052658
	Natural variant	955	1	R → G in a breast cancer sample; somatic mutation. Ref.7		VAR_035808

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: AE9B136DF0FFE5B8	FASTA	956	104,201
	10 20 30 40 50 60 METQELRGAL ALLLLCFFTS ASQDLQVIDL LTVGESRQMV AVAEKIRTAL LTAGDIYLLS 70 80 90 100 110 120 TFRLPPKQGG VLFGLYSRQD NTRWLEASVV GKINKVLVRY QREDGKVHAV NLQQAGLADG 130 140 150 160 170 180 RTHTVLLRLR GPSRPSPALH LYVDCKLGDQ HAGLPALAPI PPAEVDGLEI RTGQKAYLRM 190 200 210 220 230 240 QGFVESMKII LGGSMARVGA LSECPFQGDE SIHSAVTNAL HSILGEQTKA LVTQLTLFNQ 250 260 270 280 290 300 ILVELRDDIR DQVKEMSLIR NTIMECQVCG FHEQRSHCSP NPCFRGVDCM EVYEYPGYRC 310 320 330 340 350 360 GPCPPGLQGN GTHCSDINEC AHADPCFPGS SCINTMPGFH CEACPRGYKG TQVSGVGIDY 370 380 390 400 410 420 ARASKQVCND IDECNDGNNG GCDPNSICTN TVGSFKCGPC RLGFLGNQSQ GCLPARTCHS 430 440 450 460 470 480 PAHSPCHIHA HCLFERNGAV SCQCNVGWAG NGNVCGTDTD IDGYPDQALP CMDNNKHCKQ 490 500 510 520 530 540 DNCLLTPNSG QEDADNDGVG DQCDDDADGD GIKNVEDNCR LFPNKDQQNS DTDSFGDACD 550 560 570 580 590 600 NCPNVPNNDQ KDTDGNGEGD ACDNDVDGDG IPNGLDNCPK VPNPLQTDRD EDGVGDACDS 610 620 630 640 650 660 CPEMSNPTQT DADSDLVGDV CDTNEDSDGD GHQDTKDNCP QLPNSSQLDS DNDGLGDECD 670 680 690 700 710 720 GDDDNDGIPD YVPPGPDNCR LVPNPNQKDS DGNGVGDVCE DDFDNDAVVD PLDVCPESAE 730 740 750 760 770 780 VTLTDFRAYQ TVVLDPEGDA QIDPNWVVLN QGMEIVQTMN SDPGLAVGYT AFNGVDFEGT 790 800 810 820 830 840 FHVNTVTDDD YAGFLFSYQD SGRFYVVMWK QTEQTYWQAT PFRAVAQPGL QLKAVTSVSG 850 860 870 880 890 900 PGEHLRNALW HTGHTPDQVR LLWTDPRNVG WRDKTSYRWQ LLHRPQVGYI RVKLYEGPQL 910 920 930 940 950 VADSGVIIDT SMRGGRLGVF CFSQENIIWS NLQYRCNDTV PEDFEPFRRQ LLQGRV
	Isoform 2 [UniParc]. Checksum: 8D7AA90E4B21CA96	FASTA	836	91,297
	10 20 30 40 50 60 METQELRGAL ALLLLCFFTS ASQDLQVIDL LTVGESRQMV AVAEKIRTAL LTAGDIYLLS 70 80 90 100 110 120 TFRLPPKQGG VLFGLYSRQD NTRWLEASVV GKINKVTNAL HSILGEQTKA LVTQLTLFNQ 130 140 150 160 170 180 ILVELRDDIR DQVKEMSLIR NTIMECQVCG FHEQRSHCSP NPCFRGVDCM EVYEYPGYRC 190 200 210 220 230 240 GPCPPGLQGN GTHCSDINEC AHADPCFPGS SCINTMPGFH CEACPRGYKG TQVSGVGIDY 250 260 270 280 290 300 ARASKQVCND IDECNDGNNG GCDPNSICTN TVGSFKCGPC RLGFLGNQSQ GCLPARTCHS 310 320 330 340 350 360 PAHSPCHIHA HCLFERNGAV SCQCNVGWAG NGNVCGTDTD IDGYPDQALP CMDNNKHCKQ 370 380 390 400 410 420 DNCLLTPNSG QEDADNDGVG DQCDDDADGD GIKNVEDNCR LFPNKDQQNS DTDSFGDACD 430 440 450 460 470 480 NCPNVPNNDQ KDTDGNGEGD ACDNDVDGDG IPNGLDNCPK VPNPLQTDRD EDGVGDACDS 490 500 510 520 530 540 CPEMSNPTQT DADSDLVGDV CDTNEDSDGD GHQDTKDNCP QLPNSSQLDS DNDGLGDECD 550 560 570 580 590 600 GDDDNDGIPD YVPPGPDNCR LVPNPNQKDS DGNGVGDVCE DDFDNDAVVD PLDVCPESAE 610 620 630 640 650 660 VTLTDFRAYQ TVVLDPEGDA QIDPNWVVLN QGMEIVQTMN SDPGLAVGYT AFNGVDFEGT 670 680 690 700 710 720 FHVNTVTDDD YAGFLFSYQD SGRFYVVMWK QTEQTYWQAT PFRAVAQPGL QLKAVTSVSG 730 740 750 760 770 780 PGEHLRNALW HTGHTPDQVR LLWTDPRNVG WRDKTSYRWQ LLHRPQVGYI RVKLYEGPQL 790 800 810 820 830 VADSGVIIDT SMRGGRLGVF CFSQENIIWS NLQYRCNDTV PEDFEPFRRQ LLQGRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and organization of the human thrombospondin 3 gene (THBS3)." Adolph K.W., Long G.L., Winfield S., Ginns E.I., Bornstein P. Genomics 27:329-336(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Lung.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Identification of three additional genes contiguous to the glucocerebrosidase locus on chromosome 1q21: implications for Gaucher disease." Winfield S.L., Tayebi N., Martin B.M., Ginns E.I., Sidransky E. Genome Res. 7:1020-1026(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-736.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 365-956. Tissue: Retina.
[7]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] GLY-955.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L38969 mRNA. Translation: AAC41762.1. AL713999 Genomic DNA. Translation: CAI95084.1. AF023268 Genomic DNA. Translation: AAC51818.1. AK298592 mRNA. Translation: BAG60782.1. CH471121 Genomic DNA. Translation: EAW53110.1. BC018786 mRNA. Translation: AAH18786.1.
IPI	IPI00329535.
PIR	A57121.
RefSeq	NP_001239536.1. NM_001252607.1. NP_001239537.1. NM_001252608.1. NP_009043.1. NM_007112.4.
UniGene	Hs.169875. Hs.658188.
3D structure databases
ProteinModelPortal	P49746.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000357362.
PTM databases
PhosphoSite	P49746.
Polymorphism databases
DMDM	1717814.
Proteomic databases
PaxDb	P49746.
PRIDE	P49746.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000368378; ENSP00000357362; ENSG00000169231. ENST00000457183; ENSP00000392207; ENSG00000169231.
GeneID	7059.
KEGG	hsa:7059.
UCSC	uc001fix.3. human.
Organism-specific databases
CTD	7059.
GeneCards	GC01M155165.
H-InvDB	HIX0029532.
HGNC	HGNC:11787. THBS3.
HPA	HPA006293.
MIM	188062. gene.
neXtProt	NX_P49746.
PharmGKB	PA36499.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG12793.
HOGENOM	HOG000007542.
HOVERGEN	HBG000636.
InParanoid	P49746.
KO	K04659.
OMA	GPCRLGF.
OrthoDB	EOG49W2DP.
PhylomeDB	P49746.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P49746.
Bgee	P49746.
CleanEx	HS_THBS3.
Genevestigator	P49746.
GermOnline	ENSG00000169231. Homo sapiens.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000742. EG-like_dom. IPR001881. EGF-like_Ca-bd. IPR013032. EGF-like_CS. IPR018097. EGF_Ca-bd_CS. IPR001791. Laminin_G. IPR024665. Thbs/COMP_coiled-coil. IPR003367. Thrombospondin_3-like_rpt. IPR017897. Thrombospondin_3_rpt. IPR008859. Thrombospondin_C. [Graphical view]
Pfam	PF11598. COMP. 1 hit. PF07645. EGF_CA. 2 hits. PF02412. TSP_3. 6 hits. PF05735. TSP_C. 1 hit. [Graphical view]
SMART	SM00181. EGF. 2 hits. SM00179. EGF_CA. 2 hits. SM00210. TSPN. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 2 hits.
PROSITE	PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 3 hits. PS01187. EGF_CA. 2 hits. PS50025. LAM_G_DOMAIN. False negative. PS51234. TSP3. 8 hits. PS51236. TSP_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	THBS3. human.
GenomeRNAi	7059.
NextBio	27605.
SOURCE	Search...

Entry information

Entry name

TSP3_HUMAN

Accession

Primary (citable) accession number: P49746
Secondary accession number(s): B1AVR8, B4DQ20, Q8WV34

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 127 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.