Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P49744

- TSP4_RAT

UniProt

P49744 - TSP4_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Thrombospondin-4

Gene

Thbs4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner.2 Publications

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. collagen binding Source: RGD
  3. fibronectin binding Source: RGD
  4. laminin-1 binding Source: RGD

GO - Biological processi

  1. behavioral response to pain Source: UniProtKB
  2. cell adhesion Source: UniProtKB-KW
  3. nervous system development Source: RGD
  4. neuron projection morphogenesis Source: RGD
  5. positive regulation of cell division Source: UniProtKB-KW
  6. protein homooligomerization Source: RGD
  7. regulation of tissue remodeling Source: UniProtKB
  8. response to endoplasmic reticulum stress Source: UniProtKB
  9. response to unfolded protein Source: UniProtKB-KW
  10. tissue remodeling Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Mitogen

Keywords - Biological processi

Cell adhesion, Tissue remodeling, Unfolded protein response

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-4
Gene namesi
Name:Thbs4
Synonyms:Tsp-4, Tsp4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi62046. Thbs4.

Subcellular locationi

Endoplasmic reticulum By similarity. Sarcoplasmic reticulum By similarity. Secreted By similarity. Secretedextracellular space By similarity. Secretedextracellular spaceextracellular matrix By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. extracellular matrix Source: UniProtKB
  3. extracellular space Source: UniProtKB
  4. neuromuscular junction Source: RGD
  5. proteinaceous extracellular matrix Source: RGD
  6. sarcoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Extracellular matrix, Sarcoplasmic reticulum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4242Sequence AnalysisAdd
BLAST
Chaini43 – 980938Thrombospondin-4PRO_0000035854Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi276 – 276InterchainCurated
Disulfide bondi279 – 279InterchainCurated
Disulfide bondi308 ↔ 319PROSITE-ProRule annotation
Disulfide bondi313 ↔ 328PROSITE-ProRule annotation
Disulfide bondi331 ↔ 342PROSITE-ProRule annotation
Disulfide bondi348 ↔ 359PROSITE-ProRule annotation
Disulfide bondi353 ↔ 368PROSITE-ProRule annotation
Disulfide bondi371 ↔ 395PROSITE-ProRule annotation
Disulfide bondi401 ↔ 412PROSITE-ProRule annotation
Disulfide bondi406 ↔ 421PROSITE-ProRule annotation
Disulfide bondi424 ↔ 436PROSITE-ProRule annotation
Disulfide bondi442 ↔ 456PROSITE-ProRule annotation
Disulfide bondi450 ↔ 466PROSITE-ProRule annotation
Disulfide bondi468 ↔ 480PROSITE-ProRule annotation
Disulfide bondi496 ↔ 501PROSITE-ProRule annotation
Disulfide bondi506 ↔ 526PROSITE-ProRule annotation
Disulfide bondi542 ↔ 562PROSITE-ProRule annotation
Disulfide bondi565 ↔ 585PROSITE-ProRule annotation
Disulfide bondi601 ↔ 621PROSITE-ProRule annotation
Disulfide bondi624 ↔ 644PROSITE-ProRule annotation
Glycosylationi631 – 6311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi662 ↔ 682PROSITE-ProRule annotation
Disulfide bondi702 ↔ 722PROSITE-ProRule annotation
Disulfide bondi738 ↔ 959PROSITE-ProRule annotation
Glycosylationi960 – 9601N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP49744.
PRIDEiP49744.

PTM databases

PhosphoSiteiP49744.

Expressioni

Tissue specificityi

Mainly expressed in astrocytes, and in ressponse to peripheral nerve injury, significantly up-regulated in the dorsal spinal cord (at protein level).2 Publications

Gene expression databases

GenevestigatoriP49744.

Interactioni

Subunit structurei

Homopentamer; disulfide-linked. Interacts with PTBP3 (By similarity). Interacts (via EGF-like 3; calcium-binding domain) with ATF6 and facilitates its processing, activation and nuclear translocation. Interacts with NOTCH1 (By similarity).By similarity

Protein-protein interaction databases

IntActiP49744. 1 interaction.
STRINGi10116.ENSRNOP00000017078.

Structurei

3D structure databases

ProteinModelPortaliP49744.
SMRiP49744. Positions 236-280, 622-961.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 210168Laminin G-likeAdd
BLAST
Domaini304 – 34340EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini344 – 38138EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini397 – 43438EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini438 – 48144EGF-like 4PROSITE-ProRule annotationAdd
BLAST
Repeati482 – 51433TSP type-3 1Add
BLAST
Repeati515 – 55036TSP type-3 2Add
BLAST
Repeati551 – 57323TSP type-3 3Add
BLAST
Repeati574 – 60936TSP type-3 4Add
BLAST
Repeati610 – 63223TSP type-3 5Add
BLAST
Repeati633 – 67038TSP type-3 6Add
BLAST
Repeati671 – 71040TSP type-3 7Add
BLAST
Repeati711 – 74636TSP type-3 8Add
BLAST
Domaini750 – 964215TSP C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the thrombospondin family.Curated
Contains 4 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.Curated
Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiP49744.
PhylomeDBiP49744.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PfamiPF11598. COMP. 1 hit.
PF00008. EGF. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49744-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTMITPSSKL TLTKGNKSWS STRCGAFLLL HLVLQPWQRA GAQATPQVFD
60 70 80 90 100
LLPSSSQRLN PAALQPVLTD PTLHELYVIS TFKLQSKSSA TIFGLYSSSD
110 120 130 140 150
NSKYFEFTVM GRLNKAILRY LKDDGKIHLV VFNNLQLADG RRHRILLRLS
160 170 180 190 200
NLQRGAGSVE LYLDCVQVDS VNNLPRAFSG LTQNPQAIEL RTFQRKPQDF
210 220 230 240 250
LEELKLVVRG SLFQVASLQD CFLQQSEPLA ATGTGDFNRQ FLGQMTQLNQ
260 270 280 290 300
LLGEVKDLLR QQVKETSFLR NTIAECQACG PLSFQSPTPN TLVPIAPPAP
310 320 330 340 350
PTRPTRRCDS SPCFRGVRCT DTRDGFQCGP CPDGYTGNGI TCSDVDECKY
360 370 380 390 400
HPCYPGVRCT NLAPGFRCDA CPVGFTGPMV QGVGINFAKT NKQVCTDVDE
410 420 430 440 450
CRNGACVLNS ICINTLGSYR CGPCKPGYTG DQTRGCRTER SCRNPEQNPC
460 470 480 490 500
SVHAQCIEER QGDVTCVCGV GWAGRAGYVC GKDVDIDSYP DEELPCSARN
510 520 530 540 550
CKKDNCKYVP NSGQEDADRD GIGDACDEDA DGDGILNEQD NCVLTHNVDQ
560 570 580 590 600
RNTDKDIFGD ACDNCRGVLN NDQKDTDGDG KGDACDDDMD GDGIKNILDN
610 620 630 640 650
CPRVPNRDQQ DRDGDGVGDA CDSCPDVSNP NQSDVDNDLV GDSCDTNQDS
660 670 680 690 700
DGDGHQDSTD NCPTVINSAQ LDTDKDGIGD ECDDDDDNDG MPDLFPPGPD
710 720 730 740 750
NCRLVPNPAQ EDSNNDGVGD ICEADFDQDK VIDRIDVCPE NAEITLTDFR
760 770 780 790 800
AYQTVVLDPE GDAQIDPNWV VLNQGMEIVQ TMNSDPGLAV GYTAFNGVDF
810 820 830 840 850
EGTFHVNTQT DDDYAGFIFG YQDSSSFYVV MWKQTEQTYW QATPFRAVAE
860 870 880 890 900
PGIQLKAVKS KTGPGEHLRN SLWHTGDTSD QVRLLWKDSR NVGWKDKVSY
910 920 930 940 950
RWFLQHRPQV GYIRVRFYEG SELVADSGVT IDTTMRGGRL GVFCFSQENI
960 970 980
IWSNLKYRCN DTIPEDFQEF QIQTFDRLDN
Length:980
Mass (Da):108,214
Last modified:October 1, 1996 - v1
Checksum:i056D41EB6E206FCF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89963 mRNA. Translation: CAA62002.1.
UniGeneiRn.11207.

Genome annotation databases

UCSCiRGD:62046. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89963 mRNA. Translation: CAA62002.1 .
UniGenei Rn.11207.

3D structure databases

ProteinModelPortali P49744.
SMRi P49744. Positions 236-280, 622-961.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P49744. 1 interaction.
STRINGi 10116.ENSRNOP00000017078.

PTM databases

PhosphoSitei P49744.

Proteomic databases

PaxDbi P49744.
PRIDEi P49744.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:62046. rat.

Organism-specific databases

RGDi 62046. Thbs4.

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000007542.
HOVERGENi HBG000636.
InParanoidi P49744.
PhylomeDBi P49744.

Miscellaneous databases

PROi P49744.

Gene expression databases

Genevestigatori P49744.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProi IPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view ]
Pfami PF11598. COMP. 1 hit.
PF00008. EGF. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view ]
SMARTi SM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view ]
SUPFAMi SSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEi PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Thrombospondin-4, an extracellular matrix protein expressed in the developing and adult nervous system promotes neurite outgrowth."
    Arber S., Caroni P.
    J. Cell Biol. 131:1083-1094(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Lewis.
    Tissue: Skeletal muscle.
  2. Cited for: FUNCTION, TISSUE SPECIFICITY.
  3. "Thrombospondin-4 contributes to spinal cord injury-induced changes in nociception."
    Zeng J., Kim D., Li K.W., Sharp K., Steward O., Zaucke F., Luo Z.D.
    Eur. J. Pain 17:1458-1464(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiTSP4_RAT
AccessioniPrimary (citable) accession number: P49744
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3