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P49744

- TSP4_RAT

UniProt

P49744 - TSP4_RAT

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Protein
Thrombospondin-4
Gene
Thbs4, Tsp-4, Tsp4
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner.2 Publications

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. collagen binding Source: RGD
  3. fibronectin binding Source: RGD
  4. laminin-1 binding Source: RGD

GO - Biological processi

  1. behavioral response to pain Source: UniProtKB
  2. cell adhesion Source: UniProtKB-KW
  3. nervous system development Source: RGD
  4. neuron projection morphogenesis Source: RGD
  5. positive regulation of cell division Source: UniProtKB-KW
  6. protein homooligomerization Source: RGD
  7. regulation of tissue remodeling Source: UniProtKB
  8. response to endoplasmic reticulum stress Source: UniProtKB
  9. response to unfolded protein Source: UniProtKB-KW
  10. tissue remodeling Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Mitogen

Keywords - Biological processi

Cell adhesion, Tissue remodeling, Unfolded protein response

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-4
Gene namesi
Name:Thbs4
Synonyms:Tsp-4, Tsp4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi62046. Thbs4.

Subcellular locationi

Endoplasmic reticulum By similarity. Sarcoplasmic reticulum By similarity. Secreted By similarity. Secretedextracellular space By similarity. Secretedextracellular spaceextracellular matrix By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. extracellular matrix Source: UniProtKB
  3. extracellular space Source: UniProtKB
  4. neuromuscular junction Source: RGD
  5. proteinaceous extracellular matrix Source: RGD
  6. sarcoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Extracellular matrix, Sarcoplasmic reticulum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4242 Reviewed prediction
Add
BLAST
Chaini43 – 980938Thrombospondin-4
PRO_0000035854Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi276 – 276Interchain Inferred
Disulfide bondi279 – 279Interchain Inferred
Disulfide bondi308 ↔ 319 By similarity
Disulfide bondi313 ↔ 328 By similarity
Disulfide bondi331 ↔ 342 By similarity
Disulfide bondi348 ↔ 359 By similarity
Disulfide bondi353 ↔ 368 By similarity
Disulfide bondi371 ↔ 395 By similarity
Disulfide bondi401 ↔ 412 By similarity
Disulfide bondi406 ↔ 421 By similarity
Disulfide bondi424 ↔ 436 By similarity
Disulfide bondi442 ↔ 456 By similarity
Disulfide bondi450 ↔ 466 By similarity
Disulfide bondi468 ↔ 480 By similarity
Disulfide bondi496 ↔ 501 By similarity
Disulfide bondi506 ↔ 526 By similarity
Disulfide bondi542 ↔ 562 By similarity
Disulfide bondi565 ↔ 585 By similarity
Disulfide bondi601 ↔ 621 By similarity
Disulfide bondi624 ↔ 644 By similarity
Glycosylationi631 – 6311N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi662 ↔ 682 By similarity
Disulfide bondi702 ↔ 722 By similarity
Disulfide bondi738 ↔ 959 By similarity
Glycosylationi960 – 9601N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP49744.
PRIDEiP49744.

PTM databases

PhosphoSiteiP49744.

Expressioni

Tissue specificityi

Mainly expressed in astrocytes, and in ressponse to peripheral nerve injury, significantly up-regulated in the dorsal spinal cord (at protein level).2 Publications

Gene expression databases

GenevestigatoriP49744.

Interactioni

Subunit structurei

Homopentamer; disulfide-linked. Interacts with PTBP3 By similarity. Interacts (via EGF-like 3; calcium-binding domain) with ATF6 and facilitates its processing, activation and nuclear translocation. Interacts with NOTCH1 By similarity.

Protein-protein interaction databases

IntActiP49744. 1 interaction.
STRINGi10116.ENSRNOP00000017078.

Structurei

3D structure databases

ProteinModelPortaliP49744.
SMRiP49744. Positions 236-280, 622-961.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 210168Laminin G-like
Add
BLAST
Domaini304 – 34340EGF-like 1
Add
BLAST
Domaini344 – 38138EGF-like 2; calcium-binding Reviewed prediction
Add
BLAST
Domaini397 – 43438EGF-like 3; calcium-binding Reviewed prediction
Add
BLAST
Domaini438 – 48144EGF-like 4
Add
BLAST
Repeati482 – 51433TSP type-3 1
Add
BLAST
Repeati515 – 55036TSP type-3 2
Add
BLAST
Repeati551 – 57323TSP type-3 3
Add
BLAST
Repeati574 – 60936TSP type-3 4
Add
BLAST
Repeati610 – 63223TSP type-3 5
Add
BLAST
Repeati633 – 67038TSP type-3 6
Add
BLAST
Repeati671 – 71040TSP type-3 7
Add
BLAST
Repeati711 – 74636TSP type-3 8
Add
BLAST
Domaini750 – 964215TSP C-terminal
Add
BLAST

Sequence similaritiesi

Belongs to the thrombospondin family.
Contains 4 EGF-like domains.
Contains 8 TSP type-3 repeats.

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiP49744.
PhylomeDBiP49744.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PfamiPF11598. COMP. 1 hit.
PF00008. EGF. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49744-1 [UniParc]FASTAAdd to Basket

« Hide

MTMITPSSKL TLTKGNKSWS STRCGAFLLL HLVLQPWQRA GAQATPQVFD    50
LLPSSSQRLN PAALQPVLTD PTLHELYVIS TFKLQSKSSA TIFGLYSSSD 100
NSKYFEFTVM GRLNKAILRY LKDDGKIHLV VFNNLQLADG RRHRILLRLS 150
NLQRGAGSVE LYLDCVQVDS VNNLPRAFSG LTQNPQAIEL RTFQRKPQDF 200
LEELKLVVRG SLFQVASLQD CFLQQSEPLA ATGTGDFNRQ FLGQMTQLNQ 250
LLGEVKDLLR QQVKETSFLR NTIAECQACG PLSFQSPTPN TLVPIAPPAP 300
PTRPTRRCDS SPCFRGVRCT DTRDGFQCGP CPDGYTGNGI TCSDVDECKY 350
HPCYPGVRCT NLAPGFRCDA CPVGFTGPMV QGVGINFAKT NKQVCTDVDE 400
CRNGACVLNS ICINTLGSYR CGPCKPGYTG DQTRGCRTER SCRNPEQNPC 450
SVHAQCIEER QGDVTCVCGV GWAGRAGYVC GKDVDIDSYP DEELPCSARN 500
CKKDNCKYVP NSGQEDADRD GIGDACDEDA DGDGILNEQD NCVLTHNVDQ 550
RNTDKDIFGD ACDNCRGVLN NDQKDTDGDG KGDACDDDMD GDGIKNILDN 600
CPRVPNRDQQ DRDGDGVGDA CDSCPDVSNP NQSDVDNDLV GDSCDTNQDS 650
DGDGHQDSTD NCPTVINSAQ LDTDKDGIGD ECDDDDDNDG MPDLFPPGPD 700
NCRLVPNPAQ EDSNNDGVGD ICEADFDQDK VIDRIDVCPE NAEITLTDFR 750
AYQTVVLDPE GDAQIDPNWV VLNQGMEIVQ TMNSDPGLAV GYTAFNGVDF 800
EGTFHVNTQT DDDYAGFIFG YQDSSSFYVV MWKQTEQTYW QATPFRAVAE 850
PGIQLKAVKS KTGPGEHLRN SLWHTGDTSD QVRLLWKDSR NVGWKDKVSY 900
RWFLQHRPQV GYIRVRFYEG SELVADSGVT IDTTMRGGRL GVFCFSQENI 950
IWSNLKYRCN DTIPEDFQEF QIQTFDRLDN 980
Length:980
Mass (Da):108,214
Last modified:October 1, 1996 - v1
Checksum:i056D41EB6E206FCF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89963 mRNA. Translation: CAA62002.1.
UniGeneiRn.11207.

Genome annotation databases

UCSCiRGD:62046. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89963 mRNA. Translation: CAA62002.1 .
UniGenei Rn.11207.

3D structure databases

ProteinModelPortali P49744.
SMRi P49744. Positions 236-280, 622-961.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P49744. 1 interaction.
STRINGi 10116.ENSRNOP00000017078.

PTM databases

PhosphoSitei P49744.

Proteomic databases

PaxDbi P49744.
PRIDEi P49744.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:62046. rat.

Organism-specific databases

RGDi 62046. Thbs4.

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000007542.
HOVERGENi HBG000636.
InParanoidi P49744.
PhylomeDBi P49744.

Miscellaneous databases

PROi P49744.

Gene expression databases

Genevestigatori P49744.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view ]
Pfami PF11598. COMP. 1 hit.
PF00008. EGF. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view ]
SMARTi SM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view ]
SUPFAMi SSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEi PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Thrombospondin-4, an extracellular matrix protein expressed in the developing and adult nervous system promotes neurite outgrowth."
    Arber S., Caroni P.
    J. Cell Biol. 131:1083-1094(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Lewis.
    Tissue: Skeletal muscle.
  2. Cited for: FUNCTION, TISSUE SPECIFICITY.
  3. "Thrombospondin-4 contributes to spinal cord injury-induced changes in nociception."
    Zeng J., Kim D., Li K.W., Sharp K., Steward O., Zaucke F., Luo Z.D.
    Eur. J. Pain 17:1458-1464(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiTSP4_RAT
AccessioniPrimary (citable) accession number: P49744
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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