Thrombospondin-4 precursor - Rattus norvegicus (Rat)

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P49744 (TSP4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Thrombospondin-4

Gene names

Name:	Thbs4
Synonyms:	Tsp-4, Tsp4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	980 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function

Acts as an extracellular mitogen and stimulates early erythroid progenitors proliferation in presence of EPO. Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen By similarity.

Subunit structure

Homopentamer; disulfide-linked. Interacts with PTBP3 By similarity.

Sequence similarities

Belongs to the thrombospondin family.

Contains 4 EGF-like domains.

Contains 1 laminin G-like domain.

Contains 1 TSP C-terminal (TSPC) domain.

Contains 8 TSP type-3 repeats.

Ontologies

Keywords
Biological process	Cell adhesion
Domain	EGF-like domain Repeat Signal
Ligand	Calcium
Molecular function	Growth factor Mitogen
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW neuron projection morphogenesis Inferred from direct assay Ref.1. Source: RGD positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW protein homooligomerization Inferred from direct assay PubMed 10956668. Source: RGD
Cellular_component	neuromuscular junction Inferred from direct assay Ref.1. Source: RGD proteinaceous extracellular matrix Inferred from direct assay Ref.1. Source: RGD
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro collagen binding Inferred from direct assay PubMed 10956668. Source: RGD fibronectin binding Inferred from direct assay PubMed 10956668. Source: RGD laminin-1 binding Inferred from direct assay PubMed 10956668. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 42

Potential

Chain

43 – 980

938

Thrombospondin-4

PRO_0000035854

Regions

Domain

43 – 210

168

Laminin G-like

Domain

304 – 343

EGF-like 1

Domain

344 – 381

EGF-like 2; calcium-binding Potential

Domain

397 – 434

EGF-like 3; calcium-binding Potential

Domain

438 – 481

EGF-like 4

Repeat

482 – 514

TSP type-3 1

Repeat

515 – 550

TSP type-3 2

Repeat

551 – 573

TSP type-3 3

Repeat

574 – 609

TSP type-3 4

Repeat

610 – 632

TSP type-3 5

Repeat

633 – 670

TSP type-3 6

Repeat

671 – 710

TSP type-3 7

Repeat

711 – 746

TSP type-3 8

Domain

750 – 964

215

TSP C-terminal

Amino acid modifications

Glycosylation

631

N-linked (GlcNAc...) Potential

Glycosylation

960

N-linked (GlcNAc...) Potential

Disulfide bond

276

Interchain Probable

Disulfide bond

279

Interchain Probable

Disulfide bond

308 ↔ 319

By similarity

Disulfide bond

313 ↔ 328

By similarity

Disulfide bond

331 ↔ 342

By similarity

Disulfide bond

348 ↔ 359

By similarity

Disulfide bond

353 ↔ 368

By similarity

Disulfide bond

371 ↔ 395

By similarity

Disulfide bond

401 ↔ 412

By similarity

Disulfide bond

406 ↔ 421

By similarity

Disulfide bond

424 ↔ 436

By similarity

Disulfide bond

442 ↔ 456

By similarity

Disulfide bond

450 ↔ 466

By similarity

Disulfide bond

468 ↔ 480

By similarity

Disulfide bond

496 ↔ 501

By similarity

Disulfide bond

506 ↔ 526

By similarity

Disulfide bond

542 ↔ 562

By similarity

Disulfide bond

565 ↔ 585

By similarity

Disulfide bond

601 ↔ 621

By similarity

Disulfide bond

624 ↔ 644

By similarity

Disulfide bond

662 ↔ 682

By similarity

Disulfide bond

702 ↔ 722

By similarity

Disulfide bond

738 ↔ 959

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P49744 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 056D41EB6E206FCF
Show »

FASTA

980

108,214

        10         20         30         40         50         60 
MTMITPSSKL TLTKGNKSWS STRCGAFLLL HLVLQPWQRA GAQATPQVFD LLPSSSQRLN 

        70         80         90        100        110        120 
PAALQPVLTD PTLHELYVIS TFKLQSKSSA TIFGLYSSSD NSKYFEFTVM GRLNKAILRY 

       130        140        150        160        170        180 
LKDDGKIHLV VFNNLQLADG RRHRILLRLS NLQRGAGSVE LYLDCVQVDS VNNLPRAFSG 

       190        200        210        220        230        240 
LTQNPQAIEL RTFQRKPQDF LEELKLVVRG SLFQVASLQD CFLQQSEPLA ATGTGDFNRQ 

       250        260        270        280        290        300 
FLGQMTQLNQ LLGEVKDLLR QQVKETSFLR NTIAECQACG PLSFQSPTPN TLVPIAPPAP 

       310        320        330        340        350        360 
PTRPTRRCDS SPCFRGVRCT DTRDGFQCGP CPDGYTGNGI TCSDVDECKY HPCYPGVRCT 

       370        380        390        400        410        420 
NLAPGFRCDA CPVGFTGPMV QGVGINFAKT NKQVCTDVDE CRNGACVLNS ICINTLGSYR 

       430        440        450        460        470        480 
CGPCKPGYTG DQTRGCRTER SCRNPEQNPC SVHAQCIEER QGDVTCVCGV GWAGRAGYVC 

       490        500        510        520        530        540 
GKDVDIDSYP DEELPCSARN CKKDNCKYVP NSGQEDADRD GIGDACDEDA DGDGILNEQD 

       550        560        570        580        590        600 
NCVLTHNVDQ RNTDKDIFGD ACDNCRGVLN NDQKDTDGDG KGDACDDDMD GDGIKNILDN 

       610        620        630        640        650        660 
CPRVPNRDQQ DRDGDGVGDA CDSCPDVSNP NQSDVDNDLV GDSCDTNQDS DGDGHQDSTD 

       670        680        690        700        710        720 
NCPTVINSAQ LDTDKDGIGD ECDDDDDNDG MPDLFPPGPD NCRLVPNPAQ EDSNNDGVGD 

       730        740        750        760        770        780 
ICEADFDQDK VIDRIDVCPE NAEITLTDFR AYQTVVLDPE GDAQIDPNWV VLNQGMEIVQ 

       790        800        810        820        830        840 
TMNSDPGLAV GYTAFNGVDF EGTFHVNTQT DDDYAGFIFG YQDSSSFYVV MWKQTEQTYW 

       850        860        870        880        890        900 
QATPFRAVAE PGIQLKAVKS KTGPGEHLRN SLWHTGDTSD QVRLLWKDSR NVGWKDKVSY 

       910        920        930        940        950        960 
RWFLQHRPQV GYIRVRFYEG SELVADSGVT IDTTMRGGRL GVFCFSQENI IWSNLKYRCN 

       970        980 
DTIPEDFQEF QIQTFDRLDN

« Hide

References

[1]	"Thrombospondin-4, an extracellular matrix protein expressed in the developing and adult nervous system promotes neurite outgrowth." Arber S., Caroni P. J. Cell Biol. 131:1083-1094(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Lewis. Tissue: Skeletal muscle.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89963 mRNA. Translation: CAA62002.1.
IPI	IPI00764543.
3D structure databases
ProteinModelPortal	P49744.
SMR	P49744. Positions 236-280, 622-961.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49744. 1 interaction.
STRING	10116.ENSRNOP00000017078.
PTM databases
PhosphoSite	P49744.
Proteomic databases
PaxDb	P49744.
PRIDE	P49744.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:62046. rat.
Organism-specific databases
RGD	62046. Thbs4.
Phylogenomic databases
eggNOG	NOG12793.
HOGENOM	HOG000007542.
HOVERGEN	HBG000636.
InParanoid	P49744.
Gene expression databases
ArrayExpress	P49744.
Genevestigator	P49744.
GermOnline	ENSRNOG00000012471. Rattus norvegicus.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000742. EG-like_dom. IPR001881. EGF-like_Ca-bd. IPR013032. EGF-like_CS. IPR018097. EGF_Ca-bd_CS. IPR001791. Laminin_G. IPR024665. Thbs/COMP_coiled-coil. IPR003367. Thrombospondin_3-like_rpt. IPR017897. Thrombospondin_3_rpt. IPR008859. Thrombospondin_C. [Graphical view]
Pfam	PF11598. COMP. 1 hit. PF00008. EGF. 1 hit. PF07645. EGF_CA. 2 hits. PF02412. TSP_3. 6 hits. PF05735. TSP_C. 1 hit. [Graphical view]
SMART	SM00181. EGF. 2 hits. SM00179. EGF_CA. 2 hits. SM00210. TSPN. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 2 hits.
PROSITE	PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 4 hits. PS01187. EGF_CA. 2 hits. PS50025. LAM_G_DOMAIN. False negative. PS51234. TSP3. 8 hits. PS51236. TSP_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TSP4_RAT

Accession

Primary (citable) accession number: P49744

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents