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P49744

- TSP4_RAT

UniProt

P49744 - TSP4_RAT

Protein

Thrombospondin-4

Gene

Thbs4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner.2 Publications

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. collagen binding Source: RGD
    3. fibronectin binding Source: RGD
    4. laminin-1 binding Source: RGD

    GO - Biological processi

    1. behavioral response to pain Source: UniProtKB
    2. cell adhesion Source: UniProtKB-KW
    3. nervous system development Source: RGD
    4. neuron projection morphogenesis Source: RGD
    5. positive regulation of cell division Source: UniProtKB-KW
    6. protein homooligomerization Source: RGD
    7. regulation of tissue remodeling Source: UniProtKB
    8. response to endoplasmic reticulum stress Source: UniProtKB
    9. response to unfolded protein Source: UniProtKB-KW
    10. tissue remodeling Source: UniProtKB-KW

    Keywords - Molecular functioni

    Growth factor, Mitogen

    Keywords - Biological processi

    Cell adhesion, Tissue remodeling, Unfolded protein response

    Keywords - Ligandi

    Calcium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thrombospondin-4
    Gene namesi
    Name:Thbs4
    Synonyms:Tsp-4, Tsp4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi62046. Thbs4.

    Subcellular locationi

    Endoplasmic reticulum By similarity. Sarcoplasmic reticulum By similarity. Secreted By similarity. Secretedextracellular space By similarity. Secretedextracellular spaceextracellular matrix By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. extracellular matrix Source: UniProtKB
    3. extracellular space Source: UniProtKB
    4. neuromuscular junction Source: RGD
    5. proteinaceous extracellular matrix Source: RGD
    6. sarcoplasmic reticulum Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Extracellular matrix, Sarcoplasmic reticulum, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4242Sequence AnalysisAdd
    BLAST
    Chaini43 – 980938Thrombospondin-4PRO_0000035854Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi276 – 276InterchainCurated
    Disulfide bondi279 – 279InterchainCurated
    Disulfide bondi308 ↔ 319PROSITE-ProRule annotation
    Disulfide bondi313 ↔ 328PROSITE-ProRule annotation
    Disulfide bondi331 ↔ 342PROSITE-ProRule annotation
    Disulfide bondi348 ↔ 359PROSITE-ProRule annotation
    Disulfide bondi353 ↔ 368PROSITE-ProRule annotation
    Disulfide bondi371 ↔ 395PROSITE-ProRule annotation
    Disulfide bondi401 ↔ 412PROSITE-ProRule annotation
    Disulfide bondi406 ↔ 421PROSITE-ProRule annotation
    Disulfide bondi424 ↔ 436PROSITE-ProRule annotation
    Disulfide bondi442 ↔ 456PROSITE-ProRule annotation
    Disulfide bondi450 ↔ 466PROSITE-ProRule annotation
    Disulfide bondi468 ↔ 480PROSITE-ProRule annotation
    Disulfide bondi496 ↔ 501PROSITE-ProRule annotation
    Disulfide bondi506 ↔ 526PROSITE-ProRule annotation
    Disulfide bondi542 ↔ 562PROSITE-ProRule annotation
    Disulfide bondi565 ↔ 585PROSITE-ProRule annotation
    Disulfide bondi601 ↔ 621PROSITE-ProRule annotation
    Disulfide bondi624 ↔ 644PROSITE-ProRule annotation
    Glycosylationi631 – 6311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi662 ↔ 682PROSITE-ProRule annotation
    Disulfide bondi702 ↔ 722PROSITE-ProRule annotation
    Disulfide bondi738 ↔ 959PROSITE-ProRule annotation
    Glycosylationi960 – 9601N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP49744.
    PRIDEiP49744.

    PTM databases

    PhosphoSiteiP49744.

    Expressioni

    Tissue specificityi

    Mainly expressed in astrocytes, and in ressponse to peripheral nerve injury, significantly up-regulated in the dorsal spinal cord (at protein level).2 Publications

    Gene expression databases

    GenevestigatoriP49744.

    Interactioni

    Subunit structurei

    Homopentamer; disulfide-linked. Interacts with PTBP3 By similarity. Interacts (via EGF-like 3; calcium-binding domain) with ATF6 and facilitates its processing, activation and nuclear translocation. Interacts with NOTCH1 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP49744. 1 interaction.
    STRINGi10116.ENSRNOP00000017078.

    Structurei

    3D structure databases

    ProteinModelPortaliP49744.
    SMRiP49744. Positions 236-280, 622-961.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 210168Laminin G-likeAdd
    BLAST
    Domaini304 – 34340EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini344 – 38138EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini397 – 43438EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini438 – 48144EGF-like 4PROSITE-ProRule annotationAdd
    BLAST
    Repeati482 – 51433TSP type-3 1Add
    BLAST
    Repeati515 – 55036TSP type-3 2Add
    BLAST
    Repeati551 – 57323TSP type-3 3Add
    BLAST
    Repeati574 – 60936TSP type-3 4Add
    BLAST
    Repeati610 – 63223TSP type-3 5Add
    BLAST
    Repeati633 – 67038TSP type-3 6Add
    BLAST
    Repeati671 – 71040TSP type-3 7Add
    BLAST
    Repeati711 – 74636TSP type-3 8Add
    BLAST
    Domaini750 – 964215TSP C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the thrombospondin family.Curated
    Contains 4 EGF-like domains.PROSITE-ProRule annotation
    Contains 1 laminin G-like domain.Curated
    Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
    Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG12793.
    HOGENOMiHOG000007542.
    HOVERGENiHBG000636.
    InParanoidiP49744.
    PhylomeDBiP49744.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    4.10.1080.10. 2 hits.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR018097. EGF_Ca-bd_CS.
    IPR001791. Laminin_G.
    IPR024665. Thbs/COMP_coiled-coil.
    IPR003367. Thrombospondin_3-like_rpt.
    IPR017897. Thrombospondin_3_rpt.
    IPR008859. Thrombospondin_C.
    IPR028974. TSP_type-3_rpt.
    [Graphical view]
    PfamiPF11598. COMP. 1 hit.
    PF00008. EGF. 1 hit.
    PF07645. EGF_CA. 2 hits.
    PF02412. TSP_3. 6 hits.
    PF05735. TSP_C. 1 hit.
    [Graphical view]
    SMARTiSM00181. EGF. 2 hits.
    SM00179. EGF_CA. 2 hits.
    SM00210. TSPN. 1 hit.
    [Graphical view]
    SUPFAMiSSF103647. SSF103647. 3 hits.
    SSF49899. SSF49899. 2 hits.
    PROSITEiPS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 4 hits.
    PS01187. EGF_CA. 2 hits.
    PS51234. TSP3. 8 hits.
    PS51236. TSP_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49744-1 [UniParc]FASTAAdd to Basket

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    MTMITPSSKL TLTKGNKSWS STRCGAFLLL HLVLQPWQRA GAQATPQVFD    50
    LLPSSSQRLN PAALQPVLTD PTLHELYVIS TFKLQSKSSA TIFGLYSSSD 100
    NSKYFEFTVM GRLNKAILRY LKDDGKIHLV VFNNLQLADG RRHRILLRLS 150
    NLQRGAGSVE LYLDCVQVDS VNNLPRAFSG LTQNPQAIEL RTFQRKPQDF 200
    LEELKLVVRG SLFQVASLQD CFLQQSEPLA ATGTGDFNRQ FLGQMTQLNQ 250
    LLGEVKDLLR QQVKETSFLR NTIAECQACG PLSFQSPTPN TLVPIAPPAP 300
    PTRPTRRCDS SPCFRGVRCT DTRDGFQCGP CPDGYTGNGI TCSDVDECKY 350
    HPCYPGVRCT NLAPGFRCDA CPVGFTGPMV QGVGINFAKT NKQVCTDVDE 400
    CRNGACVLNS ICINTLGSYR CGPCKPGYTG DQTRGCRTER SCRNPEQNPC 450
    SVHAQCIEER QGDVTCVCGV GWAGRAGYVC GKDVDIDSYP DEELPCSARN 500
    CKKDNCKYVP NSGQEDADRD GIGDACDEDA DGDGILNEQD NCVLTHNVDQ 550
    RNTDKDIFGD ACDNCRGVLN NDQKDTDGDG KGDACDDDMD GDGIKNILDN 600
    CPRVPNRDQQ DRDGDGVGDA CDSCPDVSNP NQSDVDNDLV GDSCDTNQDS 650
    DGDGHQDSTD NCPTVINSAQ LDTDKDGIGD ECDDDDDNDG MPDLFPPGPD 700
    NCRLVPNPAQ EDSNNDGVGD ICEADFDQDK VIDRIDVCPE NAEITLTDFR 750
    AYQTVVLDPE GDAQIDPNWV VLNQGMEIVQ TMNSDPGLAV GYTAFNGVDF 800
    EGTFHVNTQT DDDYAGFIFG YQDSSSFYVV MWKQTEQTYW QATPFRAVAE 850
    PGIQLKAVKS KTGPGEHLRN SLWHTGDTSD QVRLLWKDSR NVGWKDKVSY 900
    RWFLQHRPQV GYIRVRFYEG SELVADSGVT IDTTMRGGRL GVFCFSQENI 950
    IWSNLKYRCN DTIPEDFQEF QIQTFDRLDN 980
    Length:980
    Mass (Da):108,214
    Last modified:October 1, 1996 - v1
    Checksum:i056D41EB6E206FCF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89963 mRNA. Translation: CAA62002.1.
    UniGeneiRn.11207.

    Genome annotation databases

    UCSCiRGD:62046. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89963 mRNA. Translation: CAA62002.1 .
    UniGenei Rn.11207.

    3D structure databases

    ProteinModelPortali P49744.
    SMRi P49744. Positions 236-280, 622-961.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P49744. 1 interaction.
    STRINGi 10116.ENSRNOP00000017078.

    PTM databases

    PhosphoSitei P49744.

    Proteomic databases

    PaxDbi P49744.
    PRIDEi P49744.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:62046. rat.

    Organism-specific databases

    RGDi 62046. Thbs4.

    Phylogenomic databases

    eggNOGi NOG12793.
    HOGENOMi HOG000007542.
    HOVERGENi HBG000636.
    InParanoidi P49744.
    PhylomeDBi P49744.

    Miscellaneous databases

    PROi P49744.

    Gene expression databases

    Genevestigatori P49744.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    4.10.1080.10. 2 hits.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR018097. EGF_Ca-bd_CS.
    IPR001791. Laminin_G.
    IPR024665. Thbs/COMP_coiled-coil.
    IPR003367. Thrombospondin_3-like_rpt.
    IPR017897. Thrombospondin_3_rpt.
    IPR008859. Thrombospondin_C.
    IPR028974. TSP_type-3_rpt.
    [Graphical view ]
    Pfami PF11598. COMP. 1 hit.
    PF00008. EGF. 1 hit.
    PF07645. EGF_CA. 2 hits.
    PF02412. TSP_3. 6 hits.
    PF05735. TSP_C. 1 hit.
    [Graphical view ]
    SMARTi SM00181. EGF. 2 hits.
    SM00179. EGF_CA. 2 hits.
    SM00210. TSPN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103647. SSF103647. 3 hits.
    SSF49899. SSF49899. 2 hits.
    PROSITEi PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 4 hits.
    PS01187. EGF_CA. 2 hits.
    PS51234. TSP3. 8 hits.
    PS51236. TSP_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Thrombospondin-4, an extracellular matrix protein expressed in the developing and adult nervous system promotes neurite outgrowth."
      Arber S., Caroni P.
      J. Cell Biol. 131:1083-1094(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Lewis.
      Tissue: Skeletal muscle.
    2. Cited for: FUNCTION, TISSUE SPECIFICITY.
    3. "Thrombospondin-4 contributes to spinal cord injury-induced changes in nociception."
      Zeng J., Kim D., Li K.W., Sharp K., Steward O., Zaucke F., Luo Z.D.
      Eur. J. Pain 17:1458-1464(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiTSP4_RAT
    AccessioniPrimary (citable) accession number: P49744
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3