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Protein

Thrombospondin-4

Gene

Thbs4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner.2 Publications

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • collagen binding Source: RGD
  • fibronectin binding Source: RGD
  • laminin-1 binding Source: RGD

GO - Biological processi

  • behavioral response to pain Source: UniProtKB
  • cell adhesion Source: UniProtKB-KW
  • nervous system development Source: RGD
  • neuron projection morphogenesis Source: RGD
  • positive regulation of cell division Source: UniProtKB-KW
  • protein homooligomerization Source: RGD
  • regulation of tissue remodeling Source: UniProtKB
  • response to endoplasmic reticulum stress Source: UniProtKB
  • response to unfolded protein Source: UniProtKB-KW
  • tissue remodeling Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Mitogen

Keywords - Biological processi

Cell adhesion, Tissue remodeling, Unfolded protein response

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-4
Gene namesi
Name:Thbs4
Synonyms:Tsp-4, Tsp4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi62046. Thbs4.

Subcellular locationi

  • Endoplasmic reticulum By similarity
  • Sarcoplasmic reticulum By similarity
  • Secreted By similarity
  • Secretedextracellular space By similarity
  • Secretedextracellular spaceextracellular matrix By similarity

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • extracellular matrix Source: UniProtKB
  • extracellular space Source: UniProtKB
  • neuromuscular junction Source: RGD
  • proteinaceous extracellular matrix Source: RGD
  • sarcoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Extracellular matrix, Sarcoplasmic reticulum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 42Sequence analysisAdd BLAST42
ChainiPRO_000003585443 – 980Thrombospondin-4Add BLAST938

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi276InterchainCurated
Disulfide bondi279InterchainCurated
Disulfide bondi308 ↔ 319PROSITE-ProRule annotation
Disulfide bondi313 ↔ 328PROSITE-ProRule annotation
Disulfide bondi331 ↔ 342PROSITE-ProRule annotation
Disulfide bondi348 ↔ 359PROSITE-ProRule annotation
Disulfide bondi353 ↔ 368PROSITE-ProRule annotation
Disulfide bondi371 ↔ 395PROSITE-ProRule annotation
Disulfide bondi401 ↔ 412PROSITE-ProRule annotation
Disulfide bondi406 ↔ 421PROSITE-ProRule annotation
Disulfide bondi424 ↔ 436PROSITE-ProRule annotation
Disulfide bondi442 ↔ 456PROSITE-ProRule annotation
Disulfide bondi450 ↔ 466PROSITE-ProRule annotation
Disulfide bondi468 ↔ 480PROSITE-ProRule annotation
Disulfide bondi496 ↔ 501PROSITE-ProRule annotation
Disulfide bondi506 ↔ 526PROSITE-ProRule annotation
Disulfide bondi542 ↔ 562PROSITE-ProRule annotation
Disulfide bondi565 ↔ 585PROSITE-ProRule annotation
Disulfide bondi601 ↔ 621PROSITE-ProRule annotation
Disulfide bondi624 ↔ 644PROSITE-ProRule annotation
Glycosylationi631N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi662 ↔ 682PROSITE-ProRule annotation
Disulfide bondi702 ↔ 722PROSITE-ProRule annotation
Disulfide bondi738 ↔ 959PROSITE-ProRule annotation
Glycosylationi960N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP49744.
PRIDEiP49744.

PTM databases

PhosphoSitePlusiP49744.

Expressioni

Tissue specificityi

Mainly expressed in astrocytes, and in ressponse to peripheral nerve injury, significantly up-regulated in the dorsal spinal cord (at protein level).2 Publications

Interactioni

Subunit structurei

Homopentamer; disulfide-linked. Interacts with PTBP3 (By similarity). Interacts (via EGF-like 3; calcium-binding domain) with ATF6 and facilitates its processing, activation and nuclear translocation. Interacts with NOTCH1 (By similarity).By similarity

GO - Molecular functioni

  • collagen binding Source: RGD
  • fibronectin binding Source: RGD
  • laminin-1 binding Source: RGD

Protein-protein interaction databases

IntActiP49744. 1 interactor.
STRINGi10116.ENSRNOP00000062272.

Structurei

3D structure databases

ProteinModelPortaliP49744.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 210Laminin G-likeAdd BLAST168
Domaini304 – 343EGF-like 1PROSITE-ProRule annotationAdd BLAST40
Domaini344 – 381EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST38
Domaini397 – 434EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST38
Domaini438 – 481EGF-like 4PROSITE-ProRule annotationAdd BLAST44
Repeati482 – 514TSP type-3 1Add BLAST33
Repeati515 – 550TSP type-3 2Add BLAST36
Repeati551 – 573TSP type-3 3Add BLAST23
Repeati574 – 609TSP type-3 4Add BLAST36
Repeati610 – 632TSP type-3 5Add BLAST23
Repeati633 – 670TSP type-3 6Add BLAST38
Repeati671 – 710TSP type-3 7Add BLAST40
Repeati711 – 746TSP type-3 8Add BLAST36
Domaini750 – 964TSP C-terminalPROSITE-ProRule annotationAdd BLAST215

Sequence similaritiesi

Belongs to the thrombospondin family.Curated
Contains 4 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.Curated
Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFQQ. Eukaryota.
ENOG410XQKE. LUCA.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiP49744.
PhylomeDBiP49744.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PfamiPF11598. COMP. 1 hit.
PF00008. EGF. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 4 hits.
SM00179. EGF_CA. 3 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMITPSSKL TLTKGNKSWS STRCGAFLLL HLVLQPWQRA GAQATPQVFD
60 70 80 90 100
LLPSSSQRLN PAALQPVLTD PTLHELYVIS TFKLQSKSSA TIFGLYSSSD
110 120 130 140 150
NSKYFEFTVM GRLNKAILRY LKDDGKIHLV VFNNLQLADG RRHRILLRLS
160 170 180 190 200
NLQRGAGSVE LYLDCVQVDS VNNLPRAFSG LTQNPQAIEL RTFQRKPQDF
210 220 230 240 250
LEELKLVVRG SLFQVASLQD CFLQQSEPLA ATGTGDFNRQ FLGQMTQLNQ
260 270 280 290 300
LLGEVKDLLR QQVKETSFLR NTIAECQACG PLSFQSPTPN TLVPIAPPAP
310 320 330 340 350
PTRPTRRCDS SPCFRGVRCT DTRDGFQCGP CPDGYTGNGI TCSDVDECKY
360 370 380 390 400
HPCYPGVRCT NLAPGFRCDA CPVGFTGPMV QGVGINFAKT NKQVCTDVDE
410 420 430 440 450
CRNGACVLNS ICINTLGSYR CGPCKPGYTG DQTRGCRTER SCRNPEQNPC
460 470 480 490 500
SVHAQCIEER QGDVTCVCGV GWAGRAGYVC GKDVDIDSYP DEELPCSARN
510 520 530 540 550
CKKDNCKYVP NSGQEDADRD GIGDACDEDA DGDGILNEQD NCVLTHNVDQ
560 570 580 590 600
RNTDKDIFGD ACDNCRGVLN NDQKDTDGDG KGDACDDDMD GDGIKNILDN
610 620 630 640 650
CPRVPNRDQQ DRDGDGVGDA CDSCPDVSNP NQSDVDNDLV GDSCDTNQDS
660 670 680 690 700
DGDGHQDSTD NCPTVINSAQ LDTDKDGIGD ECDDDDDNDG MPDLFPPGPD
710 720 730 740 750
NCRLVPNPAQ EDSNNDGVGD ICEADFDQDK VIDRIDVCPE NAEITLTDFR
760 770 780 790 800
AYQTVVLDPE GDAQIDPNWV VLNQGMEIVQ TMNSDPGLAV GYTAFNGVDF
810 820 830 840 850
EGTFHVNTQT DDDYAGFIFG YQDSSSFYVV MWKQTEQTYW QATPFRAVAE
860 870 880 890 900
PGIQLKAVKS KTGPGEHLRN SLWHTGDTSD QVRLLWKDSR NVGWKDKVSY
910 920 930 940 950
RWFLQHRPQV GYIRVRFYEG SELVADSGVT IDTTMRGGRL GVFCFSQENI
960 970 980
IWSNLKYRCN DTIPEDFQEF QIQTFDRLDN
Length:980
Mass (Da):108,214
Last modified:October 1, 1996 - v1
Checksum:i056D41EB6E206FCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89963 mRNA. Translation: CAA62002.1.
UniGeneiRn.11207.

Genome annotation databases

UCSCiRGD:62046. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89963 mRNA. Translation: CAA62002.1.
UniGeneiRn.11207.

3D structure databases

ProteinModelPortaliP49744.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP49744. 1 interactor.
STRINGi10116.ENSRNOP00000062272.

PTM databases

PhosphoSitePlusiP49744.

Proteomic databases

PaxDbiP49744.
PRIDEiP49744.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:62046. rat.

Organism-specific databases

RGDi62046. Thbs4.

Phylogenomic databases

eggNOGiENOG410IFQQ. Eukaryota.
ENOG410XQKE. LUCA.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiP49744.
PhylomeDBiP49744.

Miscellaneous databases

PROiP49744.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PfamiPF11598. COMP. 1 hit.
PF00008. EGF. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 4 hits.
SM00179. EGF_CA. 3 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 4 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSP4_RAT
AccessioniPrimary (citable) accession number: P49744
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.