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Protein

DNA replication licensing factor Mcm2

Gene

Mcm2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri314 – 340C4-typeSequence analysisAdd BLAST27
Nucleotide bindingi508 – 515ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • chromosome condensation Source: FlyBase
  • DNA duplex unwinding Source: GOC
  • DNA replication Source: FlyBase
  • DNA replication initiation Source: InterPro
  • negative regulation of DNA helicase activity Source: InterPro

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, Cell division, DNA replication
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-176187 Activation of ATR in response to replication stress
R-DME-68867 Assembly of the pre-replicative complex
R-DME-68949 Orc1 removal from chromatin
R-DME-68962 Activation of the pre-replicative complex
R-DME-69052 Switching of origins to a post-replicative state

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor Mcm2 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance 2 protein
Short name:
DmMCM2
Gene namesi
Name:Mcm2
ORF Names:CG7538
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0014861 Mcm2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi514K → A: Reduces complex helicase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940901 – 887DNA replication licensing factor Mcm2Add BLAST887

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26Phosphothreonine1 Publication1
Modified residuei27Phosphoserine1 Publication1
Modified residuei89Phosphoserine1 Publication1
Modified residuei92Phosphoserine1 Publication1
Modified residuei124Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49735
PRIDEiP49735

PTM databases

iPTMnetiP49735

Expressioni

Gene expression databases

BgeeiFBgn0014861
GenevisibleiP49735 DM

Interactioni

Subunit structurei

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable). Interacts with Mcm10.Curated3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi66154, 13 interactors
DIPiDIP-22529N
IntActiP49735, 12 interactors
STRINGi7227.FBpp0081317

Structurei

3D structure databases

ProteinModelPortaliP49735
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini458 – 665MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi640 – 643Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri314 – 340C4-typeSequence analysisAdd BLAST27

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0477 Eukaryota
COG1241 LUCA
GeneTreeiENSGT00910000144177
InParanoidiP49735
KOiK02540
OMAiKYDCVKC
OrthoDBiEOG091G026H
PhylomeDBiP49735

Family and domain databases

InterProiView protein in InterPro
IPR031327 MCM
IPR008045 MCM2
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PTHR11630:SF44 PTHR11630:SF44, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF12619 MCM2_N, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01658 MCMPROTEIN2
SMARTiView protein in SMART
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P49735-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNPSSPPPN TPSDAAERRD LRAAMTSPVG DFEPFENEDE ILGDQTVRDE
60 70 80 90 100
AEEEDGEELF GDNMENDYRP MPELDHYDPA LLDDEDDFSE MSQGDRFAAE
110 120 130 140 150
SEMRRRDRAA GIHRDDRDLG FGQSDDEDDV GPRAKRRAGE KAAVGEVEDT
160 170 180 190 200
EMVESIENLE DTKGHSTKEW VSMLGPRTEI ANRFQSFLRT FVDERGAYTY
210 220 230 240 250
RDRIRRMCEQ NMSSFVVSYT DLANKEHVLA YFLPEAPFQM LEIFDKVAKD
260 270 280 290 300
MVLSIFPTYE RVTTEIHVRI SELPLIEELR TFRKLHLNQL VRTLGVVTAT
310 320 330 340 350
TGVLPQLSVI KYDCVKCGYV LGPFVQSQNT EIKPGSCPEC QSTGPFSINM
360 370 380 390 400
EQTLYRNYQK ITLQESPGRI PAGRIPRSKD VILLADLCDQ CKPGDELEVT
410 420 430 440 450
GIYTNNYDGS LNTDQGFPVF ATVIIANHVV VKDSKQVVQS LTDEDIATIQ
460 470 480 490 500
KLSKDPRIVE RVVASMAPSI YGHDYIKRAL ALALFGGESK NPGEKHKVRG
510 520 530 540 550
DINLLICGDP GTAKSQFLKY TEKVAPRAVF TTGQGASAVG LTAYVRRNPV
560 570 580 590 600
SREWTLEAGA LVLADQGVCL IDEFDKMNDQ DRTSIHEAME QQSISISKAG
610 620 630 640 650
IVTSLQARCT VIAAANPIGG RYDPSMTFSE NVNLSEPILS RFDVLCVVKD
660 670 680 690 700
EFDPMQDQQL AKFVVHSHMK HHPSEEEQPE LEEPQLKTVD EIPQDLLRQY
710 720 730 740 750
IVYAKENIRP KLTNIDEDKI AKMYAQLRQE SFATGSLPIT VRHIESVIRM
760 770 780 790 800
SEAHARMHLR ENVMEADVSM AIRMMLESFI EAQKFSVMKK MRSTFQKYLS
810 820 830 840 850
FQKDHSELLF FILRQLTLDQ LAYIRCKDGP GATHVEIMER DLIERAKQLD
860 870 880
IVNLKPFYES DLFRTNGFSY DPKRRIILQI VVDGNTA
Length:887
Mass (Da):100,415
Last modified:October 1, 1996 - v1
Checksum:i26A7092109F09CBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42762 mRNA Translation: AAB36617.1
AE014297 Genomic DNA Translation: AAF54207.1
AY069702 mRNA Translation: AAL39847.1
RefSeqiNP_477121.1, NM_057773.4
UniGeneiDm.1996

Genome annotation databases

EnsemblMetazoaiFBtr0081827; FBpp0081317; FBgn0014861
GeneIDi40973
KEGGidme:Dmel_CG7538

Similar proteinsi

Entry informationi

Entry nameiMCM2_DROME
AccessioniPrimary (citable) accession number: P49735
Secondary accession number(s): Q9VHU2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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