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Protein

DNA replication licensing factor mcm6

Gene

mcm6

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi476 – 483ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • double-strand break repair via homologous recombination Source: PomBase
  • mitotic DNA replication Source: PomBase
  • mitotic DNA replication initiation Source: PomBase
  • premeiotic DNA replication Source: PomBase

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-68962 Activation of the pre-replicative complex
R-SPO-69052 Switching of origins to a post-replicative state
R-SPO-69300 Removal of licensing factors from origins

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor mcm6 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 6
Gene namesi
Name:mcm6
Synonyms:mis5
ORF Names:SPBC211.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC211.04c
PomBaseiSPBC211.04c mcm6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941181 – 892DNA replication licensing factor mcm6Add BLAST892

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei96Phosphoserine1 Publication1
Modified residuei98Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49731
PaxDbiP49731
PRIDEiP49731

PTM databases

iPTMnetiP49731

Interactioni

Subunit structurei

Component of the mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (Probable). The heterodimers of mcm4/mcm6 and mcm3/mcm5 interact with mcm2 and mcm7. Interacts with sld3.Curated2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SPAC1687.04O944502EBI-913838,EBI-7492115

Protein-protein interaction databases

BioGridi277303, 25 interactors
IntActiP49731, 9 interactors
MINTiP49731
STRINGi4896.SPBC211.04c.1

Structurei

3D structure databases

ProteinModelPortaliP49731
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini426 – 633MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi608 – 611Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

HOGENOMiHOG000224130
InParanoidiP49731
KOiK02542
OMAiFLKYICS
OrthoDBiEOG092C0VUM
PhylomeDBiP49731

Family and domain databases

InterProiView protein in InterPro
IPR031327 MCM
IPR008049 MCM6
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01662 MCMPROTEIN6
SMARTiView protein in SMART
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P49731-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLASQGNN ASTPAYRYGF QTSEVGDRPT VSMPSQPESS AMLEDDGMVK
60 70 80 90 100
RKPFAAIGEA IPKVIDTTGE SVREAFEEFL LSFSDDRVAG GDALPSASQE
110 120 130 140 150
KYYVQQIHGL AMYEIHTVYV DYKHLTSYND VLALAIVEQY YRFSPFLLRA
160 170 180 190 200
LQKLIEKFEP EYYRSSLSRE NASLSPNFKA SDKTFALAFY NLPFRSTIRD
210 220 230 240 250
LRTDRIGRLT TITGTVTRTS EVRPELAQGT FICEECHTVV SNVEQAFRYT
260 270 280 290 300
EPTQCPNELC ANKRSWRLNI SQSSFQDWQK VRIQENSNEI PTGSMPRTLD
310 320 330 340 350
VILRGDIVER AKAGDKCAFT GILIAVPDVS QLGIPGVKPE AYRDSRNFGG
360 370 380 390 400
RDADGVTGLK SLGVRDLTYK LSFLACMVQP DDANDKSGAD VRGDGSQGIE
410 420 430 440 450
EQDEFLQSLS QEEIDDLRAM VHSDHIYSRL TNSLAPSVYG HEIIKKGILL
460 470 480 490 500
QLMGGVHKLT PEGINLRGDL NICIVGDPST SKSQFLKYVC NFLPRAIYTS
510 520 530 540 550
GKASSAAGLT AAVVKDEETG DFTIEAGALM LADNGICAID EFDKMDLSDQ
560 570 580 590 600
VAIHEAMEQQ TISIAKAGIQ ATLNARTSIL AAANPIGGRY NRKTTLRNNI
610 620 630 640 650
NMSAPIMSRF DLFFVVLDEC NESVDRHLAK HIVDIHRLRD DAMQPEFSTE
660 670 680 690 700
QLQRYIRYAR TFKPKLNTES CAEIVKKYKQ LRMDDAQGAG KNSYRITVRQ
710 720 730 740 750
LESMIRLSEA IARANCVDDI TPAFVNEAYS LLRQSIIHVE RDDIEVEEDD
760 770 780 790 800
AEAQELENDN TNTTNGNDNV SSEEALQKPK VKITYDKYVS IMNGILQVLR
810 820 830 840 850
QRSTEGVDGV PAGDLVQSYL ELREDQFHTE EDIIYEVGLV RKVLTRLVHE
860 870 880 890
SIIMEIQNLT DSAVRLPFEE RVFSIHPNCD IDALLSNGDV PN
Length:892
Mass (Da):99,550
Last modified:August 14, 2001 - v2
Checksum:i3A00DB55B2EE08B7
GO

Sequence cautioni

The sequence BAA06729 differs from that shown. Reason: Frameshift at position 864.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31960 Genomic DNA Translation: BAA06729.1 Frameshift.
CU329671 Genomic DNA Translation: CAB75412.1
PIRiT43423
T50339
RefSeqiNP_596614.1, NM_001022535.2

Genome annotation databases

EnsemblFungiiSPBC211.04c.1; SPBC211.04c.1:pep; SPBC211.04c
GeneIDi2540784
KEGGispo:SPBC211.04c

Similar proteinsi

Entry informationi

Entry nameiMCM6_SCHPO
AccessioniPrimary (citable) accession number: P49731
Secondary accession number(s): Q9P7R7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: August 14, 2001
Last modified: March 28, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health