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P49730

- RIR2_TOBAC

UniProt

P49730 - RIR2_TOBAC

Protein

Ribonucleoside-diphosphate reductase small chain

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    • Comment

    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi75 – 751Iron 1PROSITE-ProRule annotation
    Metal bindingi106 – 1061Iron 1PROSITE-ProRule annotation
    Metal bindingi106 – 1061Iron 2By similarity
    Metal bindingi109 – 1091Iron 1PROSITE-ProRule annotation
    Active sitei113 – 1131PROSITE-ProRule annotation
    Metal bindingi168 – 1681Iron 2By similarity
    Metal bindingi202 – 2021Iron 2By similarity
    Metal bindingi205 – 2051Iron 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleoside-diphosphate reductase R2 subunit
    Ribonucleotide reductase small subunit
    OrganismiNicotiana tabacum (Common tobacco)
    Taxonomic identifieri4097 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329Ribonucleoside-diphosphate reductase small chainPRO_0000190467Add
    BLAST

    Expressioni

    Inductioni

    Maximum level of expression in mid-S phase.

    Interactioni

    Subunit structurei

    Heterodimer of a large and a small chain.

    Structurei

    3D structure databases

    ProteinModelPortaliP49730.
    SMRiP49730. Positions 2-287.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    SUPFAMiSSF47240. SSF47240. 1 hit.
    PROSITEiPS00368. RIBORED_SMALL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P49730-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLIPEEPLL ASSPDRFCMF PIQYPQIWEM YKKALASFWT AEEVDLSSDT    50
    RHWETLTPGE RHFITHVLAF FAASDGIVLE NLAGRFMKEV QVAEARAFYG 100
    FQIAIENIHS EMYSLLLESY IKDSDEKSRL FRAVETNPCV EKKAKWALRW 150
    IDGSETFAER LVAFACVEGI FFSGSFCAIF WLKKRGLMPG LTFSNELISR 200
    DEGLHCDFAC LLYSLLRTKL TEERVKGIVA DAVEIEREFV CDALPCALVG 250
    MNGDLMSKYI EFVADRLLDA LGYDKLYNAQ NPFDWMELIS LQGKTNFFEK 300
    RVGEYQKASV MSSLNGNGAT HEFKLDEDF 329
    Length:329
    Mass (Da):37,608
    Last modified:October 1, 1996 - v1
    Checksum:i65E7A4183AF045FD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92443 mRNA. Translation: CAA63194.1.
    PIRiT03688.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92443 mRNA. Translation: CAA63194.1 .
    PIRi T03688.

    3D structure databases

    ProteinModelPortali P49730.
    SMRi P49730. Positions 2-287.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47240. SSF47240. 1 hit.
    PROSITEi PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of tobacco ribonucleotide reductase RNR1 and RNR2 cDNAs and cell cycle-regulated expression in synchronized plant cells."
      Chaboute M.-E., Combettes B., Clement B., Gigot C., Philipps G.
      Plant Mol. Biol. 38:797-806(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Bright Yellow 2.

    Entry informationi

    Entry nameiRIR2_TOBAC
    AccessioniPrimary (citable) accession number: P49730
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3