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P49730 (RIR2_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase small chain

EC=1.17.4.1
Alternative name(s):
Ribonucleoside-diphosphate reductase R2 subunit
Ribonucleotide reductase small subunit
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Heterodimer of a large and a small chain.

Subcellular location

Cytoplasm By similarity.

Induction

Maximum level of expression in mid-S phase.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentCytoplasm
   LigandIron
Metal-binding
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-UniPathway

deoxyribonucleoside diphosphate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Ribonucleoside-diphosphate reductase small chain
PRO_0000190467

Sites

Active site1131 By similarity
Metal binding751Iron 1 By similarity
Metal binding1061Iron 1 By similarity
Metal binding1061Iron 2 By similarity
Metal binding1091Iron 1 By similarity
Metal binding1681Iron 2 By similarity
Metal binding2021Iron 2 By similarity
Metal binding2051Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P49730 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 65E7A4183AF045FD

FASTA32937,608
        10         20         30         40         50         60 
MPLIPEEPLL ASSPDRFCMF PIQYPQIWEM YKKALASFWT AEEVDLSSDT RHWETLTPGE 

        70         80         90        100        110        120 
RHFITHVLAF FAASDGIVLE NLAGRFMKEV QVAEARAFYG FQIAIENIHS EMYSLLLESY 

       130        140        150        160        170        180 
IKDSDEKSRL FRAVETNPCV EKKAKWALRW IDGSETFAER LVAFACVEGI FFSGSFCAIF 

       190        200        210        220        230        240 
WLKKRGLMPG LTFSNELISR DEGLHCDFAC LLYSLLRTKL TEERVKGIVA DAVEIEREFV 

       250        260        270        280        290        300 
CDALPCALVG MNGDLMSKYI EFVADRLLDA LGYDKLYNAQ NPFDWMELIS LQGKTNFFEK 

       310        320 
RVGEYQKASV MSSLNGNGAT HEFKLDEDF 

« Hide

References

[1]"Molecular characterization of tobacco ribonucleotide reductase RNR1 and RNR2 cDNAs and cell cycle-regulated expression in synchronized plant cells."
Chaboute M.-E., Combettes B., Clement B., Gigot C., Philipps G.
Plant Mol. Biol. 38:797-806(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Bright Yellow 2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X92443 mRNA. Translation: CAA63194.1.
PIRT03688.

3D structure databases

ProteinModelPortalP49730.
SMRP49730. Positions 2-287.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00326.

Family and domain databases

Gene3D1.10.620.20. 1 hit.
InterProIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERPTHR23409. PTHR23409. 1 hit.
PfamPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMSSF47240. SSF47240. 1 hit.
PROSITEPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR2_TOBAC
AccessionPrimary (citable) accession number: P49730
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways