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Protein

Ribonucleoside-diphosphate reductase small chain

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi75 – 751Iron 1PROSITE-ProRule annotation
Metal bindingi106 – 1061Iron 1PROSITE-ProRule annotation
Metal bindingi106 – 1061Iron 2By similarity
Metal bindingi109 – 1091Iron 1PROSITE-ProRule annotation
Active sitei113 – 1131PROSITE-ProRule annotation
Metal bindingi168 – 1681Iron 2By similarity
Metal bindingi202 – 2021Iron 2By similarity
Metal bindingi205 – 2051Iron 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase R2 subunit
Ribonucleotide reductase small subunit
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Ribonucleoside-diphosphate reductase small chainPRO_0000190467Add
BLAST

Expressioni

Inductioni

Maximum level of expression in mid-S phase.

Interactioni

Subunit structurei

Heterodimer of a large and a small chain.

Structurei

3D structure databases

ProteinModelPortaliP49730.
SMRiP49730. Positions 2-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
IPR030475. RNR_small_AS.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49730-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLIPEEPLL ASSPDRFCMF PIQYPQIWEM YKKALASFWT AEEVDLSSDT
60 70 80 90 100
RHWETLTPGE RHFITHVLAF FAASDGIVLE NLAGRFMKEV QVAEARAFYG
110 120 130 140 150
FQIAIENIHS EMYSLLLESY IKDSDEKSRL FRAVETNPCV EKKAKWALRW
160 170 180 190 200
IDGSETFAER LVAFACVEGI FFSGSFCAIF WLKKRGLMPG LTFSNELISR
210 220 230 240 250
DEGLHCDFAC LLYSLLRTKL TEERVKGIVA DAVEIEREFV CDALPCALVG
260 270 280 290 300
MNGDLMSKYI EFVADRLLDA LGYDKLYNAQ NPFDWMELIS LQGKTNFFEK
310 320
RVGEYQKASV MSSLNGNGAT HEFKLDEDF
Length:329
Mass (Da):37,608
Last modified:October 1, 1996 - v1
Checksum:i65E7A4183AF045FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92443 mRNA. Translation: CAA63194.1.
PIRiT03688.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92443 mRNA. Translation: CAA63194.1.
PIRiT03688.

3D structure databases

ProteinModelPortaliP49730.
SMRiP49730. Positions 2-287.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
IPR030475. RNR_small_AS.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of tobacco ribonucleotide reductase RNR1 and RNR2 cDNAs and cell cycle-regulated expression in synchronized plant cells."
    Chaboute M.-E., Combettes B., Clement B., Gigot C., Philipps G.
    Plant Mol. Biol. 38:797-806(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Bright Yellow 2.

Entry informationi

Entry nameiRIR2_TOBAC
AccessioniPrimary (citable) accession number: P49730
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 1, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.