ID UCRIA_CHLRE Reviewed; 206 AA. AC P49728; DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1996, sequence version 1. DT 16-JUN-2009, entry version 69. DE RecName: Full=Cytochrome b6-f complex iron-sulfur subunit, chloroplastic; DE EC=1.10.99.1; DE AltName: Full=Rieske iron-sulfur protein; DE Short=RISP; DE Short=ISP; DE AltName: Full=Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein; DE Flags: Precursor; GN Name=petC; OS Chlamydomonas reinhardtii. OC Eukaryota; Viridiplantae; Chlorophyta; Chlorophyceae; OC Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas. OX NCBI_TaxID=3055; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=cw15; RX MEDLINE=94171792; PubMed=8125984; RA de Vitry C.; RT "Characterization of the gene of the chloroplast Rieske iron-sulfur RT protein in Chlamydomonas reinhardtii. Indications for an uncleaved RT lumen targeting sequence."; RL J. Biol. Chem. 269:7603-7609(1994). RN [2] RP NUCLEOTIDE SEQUENCE. RC STRAIN=2137; RA Takahashi Y., Kataoka S., Matsubara H., Malkin R.; RT "A cDNA encoding chloroplast Rieske protein from Chlamydomonas RT reinhardtii."; RL Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases. RN [3] RP PROTEIN SEQUENCE OF 30-56 AND 168-186, AND CHARACTERIZATION. RC STRAIN=WT12; RX MEDLINE=96094329; PubMed=7493968; DOI=10.1074/jbc.270.49.29342; RA Pierre Y., Breyton C., Kramer D., Popot J.-L.; RT "Purification and characterization of the cytochrome b6 f complex from RT Chlamydomonas reinhardtii."; RL J. Biol. Chem. 270:29342-29349(1995). RN [4] RP X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 31-206. RX PubMed=14647374; DOI=10.1038/nature02155; RA Stroebel D., Choquet Y., Popot J.-L., Picot D.; RT "An atypical haem in the cytochrome b(6)f complex."; RL Nature 426:413-418(2003). CC -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates CC electron transfer between photosystem II (PSII) and photosystem I CC (PSI), cyclic electron flow around PSI, and state transitions. CC -!- CATALYTIC ACTIVITY: Plastoquinol-1 + 2 oxidized plastocyanin = CC plastoquinone + 2 reduced plastocyanin. CC -!- COFACTOR: Binds 1 2Fe-2S cluster per subunit. CC -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are CC cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f CC and the Rieske protein, while the 4 small subunits are petG, petL, CC petM and petN. The complex functions as a dimer. CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; CC Single-pass membrane protein. Note=The transmembrane helix CC obliquely spans the membrane in one monomer, and its extrinsic C- CC terminal domain is part of the other monomer. CC -!- MISCELLANEOUS: This protein is 1 of 2 subunits of the cytochrome CC b6-f complex that are encoded in the nucleus. CC -!- MISCELLANEOUS: The Rieske iron-sulfur protein is a high potential CC 2Fe-2S protein. CC -!- SIMILARITY: Contains 1 Rieske domain. CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; X76299; CAA53947.1; -; Genomic_DNA. DR EMBL; D32003; BAA22147.1; -; mRNA. DR PIR; A53412; A53412. DR RefSeq; XP_001698786.1; -. DR UniGene; Cre.13203; -. DR PDB; 1Q90; X-ray; 3.10 A; C=80-206, R=31-79. DR PDBsum; 1Q90; -. DR PRIDE; P49728; -. DR GeneID; 5724296; -. DR KEGG; cre:CHLREDRAFT_193296; -. DR BRENDA; 1.10.99.1; 144. DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell. DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW. DR GO; GO:0009055; F:electron carrier activity; IEA:InterPro. DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW. DR GO; GO:0009496; F:plastoquinol-plastocyanin reductase activity; IEA:EC. DR GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-KW. DR GO; GO:0006810; P:transport; IEA:UniProtKB-KW. DR InterPro; IPR014909; Cyt_b6-f_cplx_Fe-S_su. DR InterPro; IPR017941; Rieske_2Fe-2S. DR InterPro; IPR014349; Rieske_Fe-S_prot. DR Gene3D; G3DSA:2.102.10.10; Rieske_reg; 1. DR PANTHER; PTHR10134; Rieske; 1. DR Pfam; PF08802; CytB6-F_Fe-S; 1. DR Pfam; PF00355; Rieske; 1. DR PROSITE; PS51296; RIESKE; 1. PE 1: Evidence at protein level; KW 2Fe-2S; 3D-structure; Chloroplast; Direct protein sequencing; KW Disulfide bond; Electron transport; Iron; Iron-sulfur; Membrane; KW Metal-binding; Oxidoreductase; Plastid; Thylakoid; Transit peptide; KW Transmembrane; Transport. FT TRANSIT 1 29 Chloroplast. FT CHAIN 30 206 Cytochrome b6-f complex iron-sulfur FT subunit, chloroplastic. FT /FTId=PRO_0000030685. FT TRANSMEM 39 68 FT DOMAIN 92 188 Rieske. FT COMPBIAS 29 32 Poly-Ala. FT COMPBIAS 74 79 Poly-Gly. FT METAL 134 134 Iron-sulfur (2Fe-2S). FT METAL 136 136 Iron-sulfur (2Fe-2S); via pros nitrogen. FT METAL 152 152 Iron-sulfur (2Fe-2S). FT METAL 155 155 Iron-sulfur (2Fe-2S); via pros nitrogen. FT DISULFID 139 154 FT HELIX 39 68 FT HELIX 92 98 FT HELIX 110 112 FT STRAND 114 119 FT STRAND 123 125 FT STRAND 127 131 FT TURN 135 137 FT TURN 145 148 FT STRAND 149 151 FT TURN 153 155 FT STRAND 158 160 FT STRAND 165 169 FT STRAND 176 180 FT STRAND 188 191 FT TURN 197 199 SQ SEQUENCE 206 AA; 21526 MW; 7B2C2118E9F75974 CRC64; MAMLSSRRVA APAKASAIRR SRVMPVVRAA AASSEVPDMN KRNIMNLILA GGAGLPITTL ALGYGAFFVP PSSGGGGGGQ AAKDALGNDI KAGEWLKTHL AGDRSLSQGL KGDPTYLIVT ADSTIEKYGL NAVCTHLGCV VPWVAAENKF KCPCHGSQYN AEGKVVRGPA PLSLALAHCD VAESGLVTFS TWTETDFRTG LEPWWA //