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Protein

Cytochrome b6-f complex iron-sulfur subunit, chloroplastic

Gene

petC

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

Catalytic activityi

Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 4 H+(Side 2).

Cofactori

[2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi134Iron-sulfur (2Fe-2S)1
Metal bindingi136Iron-sulfur (2Fe-2S); via pros nitrogen1
Metal bindingi152Iron-sulfur (2Fe-2S)1
Metal bindingi155Iron-sulfur (2Fe-2S); via pros nitrogen1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:CHLREDRAFT_193296-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (EC:1.10.9.1)
Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Rieske iron-sulfur protein
Short name:
ISP
Short name:
RISP
Gene namesi
Name:petC
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei39 – 68HelicalAdd BLAST30

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29Chloroplast1 PublicationAdd BLAST29
ChainiPRO_000003068530 – 206Cytochrome b6-f complex iron-sulfur subunit, chloroplasticAdd BLAST177

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi139 ↔ 154

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP49728.

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer.

Protein-protein interaction databases

DIPiDIP-58594N.
STRINGi3055.EDO99286.

Structurei

Secondary structure

1206
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi39 – 68Combined sources30
Helixi92 – 98Combined sources7
Helixi110 – 112Combined sources3
Beta strandi114 – 119Combined sources6
Beta strandi123 – 125Combined sources3
Beta strandi127 – 131Combined sources5
Turni135 – 137Combined sources3
Turni145 – 148Combined sources4
Beta strandi149 – 151Combined sources3
Turni153 – 155Combined sources3
Beta strandi158 – 160Combined sources3
Beta strandi165 – 169Combined sources5
Beta strandi176 – 180Combined sources5
Beta strandi188 – 191Combined sources4
Turni197 – 199Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q90X-ray3.10C80-206[»]
R31-79[»]
ProteinModelPortaliP49728.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49728.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 188RieskePROSITE-ProRule annotationAdd BLAST97

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi29 – 32Poly-Ala4
Compositional biasi74 – 79Poly-Gly6

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1671. Eukaryota.
COG0723. LUCA.
KOiK02636.
OMAiKGDPTYI.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMLSSRRVA APAKASAIRR SRVMPVVRAA AASSEVPDMN KRNIMNLILA
60 70 80 90 100
GGAGLPITTL ALGYGAFFVP PSSGGGGGGQ AAKDALGNDI KAGEWLKTHL
110 120 130 140 150
AGDRSLSQGL KGDPTYLIVT ADSTIEKYGL NAVCTHLGCV VPWVAAENKF
160 170 180 190 200
KCPCHGSQYN AEGKVVRGPA PLSLALAHCD VAESGLVTFS TWTETDFRTG

LEPWWA
Length:206
Mass (Da):21,526
Last modified:October 1, 1996 - v1
Checksum:i7B2C2118E9F75974
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76299 Genomic DNA. Translation: CAA53947.1.
D32003 mRNA. Translation: BAA22147.1.
PIRiA53412.
RefSeqiXP_001698786.1. XM_001698734.1.
UniGeneiCre.13203.

Genome annotation databases

EnsemblPlantsiEDO99286; EDO99286; CHLREDRAFT_193296.
GeneIDi5724296.
GrameneiEDO99286; EDO99286; CHLREDRAFT_193296.
KEGGicre:CHLREDRAFT_193296.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76299 Genomic DNA. Translation: CAA53947.1.
D32003 mRNA. Translation: BAA22147.1.
PIRiA53412.
RefSeqiXP_001698786.1. XM_001698734.1.
UniGeneiCre.13203.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q90X-ray3.10C80-206[»]
R31-79[»]
ProteinModelPortaliP49728.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-58594N.
STRINGi3055.EDO99286.

Proteomic databases

PaxDbiP49728.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiEDO99286; EDO99286; CHLREDRAFT_193296.
GeneIDi5724296.
GrameneiEDO99286; EDO99286; CHLREDRAFT_193296.
KEGGicre:CHLREDRAFT_193296.

Phylogenomic databases

eggNOGiKOG1671. Eukaryota.
COG0723. LUCA.
KOiK02636.
OMAiKGDPTYI.

Enzyme and pathway databases

BioCyciMetaCyc:CHLREDRAFT_193296-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP49728.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUCRIA_CHLRE
AccessioniPrimary (citable) accession number: P49728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 5, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus.
The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.