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P49728

- UCRIA_CHLRE

UniProt

P49728 - UCRIA_CHLRE

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Protein
Cytochrome b6-f complex iron-sulfur subunit, chloroplastic
Gene
petC
Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

Catalytic activityi

Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 4 H+(Side 2).

Cofactori

Binds 1 2Fe-2S cluster per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi134 – 1341Iron-sulfur (2Fe-2S)
Metal bindingi136 – 1361Iron-sulfur (2Fe-2S); via pros nitrogen
Metal bindingi152 – 1521Iron-sulfur (2Fe-2S)
Metal bindingi155 – 1551Iron-sulfur (2Fe-2S); via pros nitrogen

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. plastoquinol--plastocyanin reductase activity Source: UniProtKB-EC
  4. ubiquinol-cytochrome-c reductase activity Source: InterPro

GO - Biological processi

  1. defense response to bacterium Source: EnsemblPlants/Gramene
  2. nonphotochemical quenching Source: EnsemblPlants/Gramene
  3. response to karrikin Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:CHLREDRAFT_193296-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (EC:1.10.9.1)
Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Rieske iron-sulfur protein
Short name:
ISP
Short name:
RISP
Gene namesi
Name:petC
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Subcellular locationi

Plastidchloroplast thylakoid membrane; Single-pass membrane protein
Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei39 – 6830Helical
Add
BLAST

GO - Cellular componenti

  1. chloroplast envelope Source: EnsemblPlants/Gramene
  2. chloroplast thylakoid membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2929Chloroplast1 Publication
Add
BLAST
Chaini30 – 206177Cytochrome b6-f complex iron-sulfur subunit, chloroplastic
PRO_0000030685Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi139 ↔ 154

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP49728.

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer.

Protein-protein interaction databases

DIPiDIP-58594N.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi39 – 6830
Helixi92 – 987
Helixi110 – 1123
Beta strandi114 – 1196
Beta strandi123 – 1253
Beta strandi127 – 1315
Turni135 – 1373
Turni145 – 1484
Beta strandi149 – 1513
Turni153 – 1553
Beta strandi158 – 1603
Beta strandi165 – 1695
Beta strandi176 – 1805
Beta strandi188 – 1914
Turni197 – 1993

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q90X-ray3.10C80-206[»]
R31-79[»]
ProteinModelPortaliP49728.
SMRiP49728. Positions 33-71, 80-206.

Miscellaneous databases

EvolutionaryTraceiP49728.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini92 – 18897Rieske
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi29 – 324Poly-Ala
Compositional biasi74 – 796Poly-Gly

Sequence similaritiesi

Contains 1 Rieske domain.

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0723.
KOiK02636.
OMAiPWTETDF.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49728-1 [UniParc]FASTAAdd to Basket

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MAMLSSRRVA APAKASAIRR SRVMPVVRAA AASSEVPDMN KRNIMNLILA    50
GGAGLPITTL ALGYGAFFVP PSSGGGGGGQ AAKDALGNDI KAGEWLKTHL 100
AGDRSLSQGL KGDPTYLIVT ADSTIEKYGL NAVCTHLGCV VPWVAAENKF 150
KCPCHGSQYN AEGKVVRGPA PLSLALAHCD VAESGLVTFS TWTETDFRTG 200
LEPWWA 206
Length:206
Mass (Da):21,526
Last modified:October 1, 1996 - v1
Checksum:i7B2C2118E9F75974
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76299 Genomic DNA. Translation: CAA53947.1.
D32003 mRNA. Translation: BAA22147.1.
PIRiA53412.
RefSeqiXP_001698786.1. XM_001698734.1.
UniGeneiCre.13203.

Genome annotation databases

GeneIDi5724296.
KEGGicre:CHLREDRAFT_193296.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76299 Genomic DNA. Translation: CAA53947.1 .
D32003 mRNA. Translation: BAA22147.1 .
PIRi A53412.
RefSeqi XP_001698786.1. XM_001698734.1.
UniGenei Cre.13203.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Q90 X-ray 3.10 C 80-206 [» ]
R 31-79 [» ]
ProteinModelPortali P49728.
SMRi P49728. Positions 33-71, 80-206.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-58594N.

Proteomic databases

PRIDEi P49728.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5724296.
KEGGi cre:CHLREDRAFT_193296.

Phylogenomic databases

eggNOGi COG0723.
KOi K02636.
OMAi PWTETDF.

Enzyme and pathway databases

BioCyci MetaCyc:CHLREDRAFT_193296-MONOMER.

Miscellaneous databases

EvolutionaryTracei P49728.

Family and domain databases

Gene3Di 2.102.10.10. 1 hit.
InterProi IPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view ]
PANTHERi PTHR10134. PTHR10134. 1 hit.
Pfami PF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view ]
PRINTSi PR00162. RIESKE.
SUPFAMi SSF50022. SSF50022. 1 hit.
PROSITEi PS51296. RIESKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of the gene of the chloroplast Rieske iron-sulfur protein in Chlamydomonas reinhardtii. Indications for an uncleaved lumen targeting sequence."
    de Vitry C.
    J. Biol. Chem. 269:7603-7609(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cw15.
  2. "A cDNA encoding chloroplast Rieske protein from Chlamydomonas reinhardtii."
    Takahashi Y., Kataoka S., Matsubara H., Malkin R.
    Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 2137.
  3. "Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii."
    Pierre Y., Breyton C., Kramer D., Popot J.-L.
    J. Biol. Chem. 270:29342-29349(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-56 AND 168-186, CHARACTERIZATION.
    Strain: WT12.
  4. "An atypical haem in the cytochrome b(6)f complex."
    Stroebel D., Choquet Y., Popot J.-L., Picot D.
    Nature 426:413-418(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 31-206.

Entry informationi

Entry nameiUCRIA_CHLRE
AccessioniPrimary (citable) accession number: P49728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus.
The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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