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P49728

- UCRIA_CHLRE

UniProt

P49728 - UCRIA_CHLRE

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Protein

Cytochrome b6-f complex iron-sulfur subunit, chloroplastic

Gene

petC

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

Catalytic activityi

Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 4 H+(Side 2).

Cofactori

Binds 1 2Fe-2S cluster per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi134 – 1341Iron-sulfur (2Fe-2S)
Metal bindingi136 – 1361Iron-sulfur (2Fe-2S); via pros nitrogen
Metal bindingi152 – 1521Iron-sulfur (2Fe-2S)
Metal bindingi155 – 1551Iron-sulfur (2Fe-2S); via pros nitrogen

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. electron transporter, transferring electrons from cytochrome b6/f complex of photosystem II activity Source: EnsemblPlants/Gramene
  3. electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Source: EnsemblPlants/Gramene
  4. metal ion binding Source: UniProtKB-KW
  5. plastoquinol--plastocyanin reductase activity Source: UniProtKB-EC
  6. ubiquinol-cytochrome-c reductase activity Source: EnsemblPlants/Gramene

GO - Biological processi

  1. defense response to fungus Source: EnsemblPlants/Gramene
  2. detection of biotic stimulus Source: EnsemblPlants/Gramene
  3. jasmonic acid mediated signaling pathway Source: EnsemblPlants/Gramene
  4. MAPK cascade Source: EnsemblPlants/Gramene
  5. negative regulation of defense response Source: EnsemblPlants/Gramene
  6. nonphotochemical quenching Source: EnsemblPlants/Gramene
  7. pentose-phosphate shunt Source: EnsemblPlants/Gramene
  8. photosynthetic electron transport chain Source: EnsemblPlants/Gramene
  9. photosystem II assembly Source: EnsemblPlants/Gramene
  10. plastid organization Source: EnsemblPlants/Gramene
  11. protein targeting to membrane Source: EnsemblPlants/Gramene
  12. regulation of hydrogen peroxide metabolic process Source: EnsemblPlants/Gramene
  13. regulation of multi-organism process Source: EnsemblPlants/Gramene
  14. regulation of plant-type hypersensitive response Source: EnsemblPlants/Gramene
  15. response to chitin Source: EnsemblPlants/Gramene
  16. response to cold Source: EnsemblPlants/Gramene
  17. rRNA processing Source: EnsemblPlants/Gramene
  18. salicylic acid biosynthetic process Source: EnsemblPlants/Gramene
  19. systemic acquired resistance, salicylic acid mediated signaling pathway Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:CHLREDRAFT_193296-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (EC:1.10.9.1)
Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Rieske iron-sulfur protein
Short name:
ISP
Short name:
RISP
Gene namesi
Name:petC
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Subcellular locationi

Plastidchloroplast thylakoid membrane; Single-pass membrane protein
Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer.

GO - Cellular componenti

  1. chloroplast envelope Source: EnsemblPlants/Gramene
  2. chloroplast thylakoid membrane Source: EnsemblPlants/Gramene
  3. cytochrome b6f complex Source: EnsemblPlants/Gramene
  4. integral component of membrane Source: UniProtKB-KW
  5. plasma membrane Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2929Chloroplast1 PublicationAdd
BLAST
Chaini30 – 206177Cytochrome b6-f complex iron-sulfur subunit, chloroplasticPRO_0000030685Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi139 ↔ 154

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP49728.

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer.

Protein-protein interaction databases

DIPiDIP-58594N.

Structurei

Secondary structure

1
206
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi39 – 6830Combined sources
Helixi92 – 987Combined sources
Helixi110 – 1123Combined sources
Beta strandi114 – 1196Combined sources
Beta strandi123 – 1253Combined sources
Beta strandi127 – 1315Combined sources
Turni135 – 1373Combined sources
Turni145 – 1484Combined sources
Beta strandi149 – 1513Combined sources
Turni153 – 1553Combined sources
Beta strandi158 – 1603Combined sources
Beta strandi165 – 1695Combined sources
Beta strandi176 – 1805Combined sources
Beta strandi188 – 1914Combined sources
Turni197 – 1993Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q90X-ray3.10C80-206[»]
R31-79[»]
ProteinModelPortaliP49728.
SMRiP49728. Positions 33-71, 80-206.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49728.

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei39 – 6830HelicalAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini92 – 18897RieskePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi29 – 324Poly-Ala
Compositional biasi74 – 796Poly-Gly

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0723.
KOiK02636.
OMAiPWTETDF.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49728-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAMLSSRRVA APAKASAIRR SRVMPVVRAA AASSEVPDMN KRNIMNLILA
60 70 80 90 100
GGAGLPITTL ALGYGAFFVP PSSGGGGGGQ AAKDALGNDI KAGEWLKTHL
110 120 130 140 150
AGDRSLSQGL KGDPTYLIVT ADSTIEKYGL NAVCTHLGCV VPWVAAENKF
160 170 180 190 200
KCPCHGSQYN AEGKVVRGPA PLSLALAHCD VAESGLVTFS TWTETDFRTG

LEPWWA
Length:206
Mass (Da):21,526
Last modified:October 1, 1996 - v1
Checksum:i7B2C2118E9F75974
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76299 Genomic DNA. Translation: CAA53947.1.
D32003 mRNA. Translation: BAA22147.1.
PIRiA53412.
RefSeqiXP_001698786.1. XM_001698734.1.
UniGeneiCre.13203.

Genome annotation databases

GeneIDi5724296.
KEGGicre:CHLREDRAFT_193296.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76299 Genomic DNA. Translation: CAA53947.1 .
D32003 mRNA. Translation: BAA22147.1 .
PIRi A53412.
RefSeqi XP_001698786.1. XM_001698734.1.
UniGenei Cre.13203.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Q90 X-ray 3.10 C 80-206 [» ]
R 31-79 [» ]
ProteinModelPortali P49728.
SMRi P49728. Positions 33-71, 80-206.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-58594N.

Proteomic databases

PRIDEi P49728.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5724296.
KEGGi cre:CHLREDRAFT_193296.

Phylogenomic databases

eggNOGi COG0723.
KOi K02636.
OMAi PWTETDF.

Enzyme and pathway databases

BioCyci MetaCyc:CHLREDRAFT_193296-MONOMER.

Miscellaneous databases

EvolutionaryTracei P49728.

Family and domain databases

Gene3Di 2.102.10.10. 1 hit.
InterProi IPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view ]
PANTHERi PTHR10134. PTHR10134. 1 hit.
Pfami PF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view ]
PRINTSi PR00162. RIESKE.
SUPFAMi SSF50022. SSF50022. 1 hit.
PROSITEi PS51296. RIESKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of the gene of the chloroplast Rieske iron-sulfur protein in Chlamydomonas reinhardtii. Indications for an uncleaved lumen targeting sequence."
    de Vitry C.
    J. Biol. Chem. 269:7603-7609(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cw15.
  2. "A cDNA encoding chloroplast Rieske protein from Chlamydomonas reinhardtii."
    Takahashi Y., Kataoka S., Matsubara H., Malkin R.
    Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 2137.
  3. "Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii."
    Pierre Y., Breyton C., Kramer D., Popot J.-L.
    J. Biol. Chem. 270:29342-29349(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-56 AND 168-186, CHARACTERIZATION.
    Strain: WT12.
  4. "An atypical haem in the cytochrome b(6)f complex."
    Stroebel D., Choquet Y., Popot J.-L., Picot D.
    Nature 426:413-418(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 31-206.

Entry informationi

Entry nameiUCRIA_CHLRE
AccessioniPrimary (citable) accession number: P49728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus.
The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3