Cytochrome b6-f complex iron-sulfur subunit, chloroplastic precursor

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Reviewed, UniProtKB/Swiss-Prot P49728 (UCRIA_CHLRE)

Last modified November 25, 2008. Version 64. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Cytochrome b6-f complex iron-sulfur subunit, chloroplastic
    EC=1.10.99.1
Alternative name(s):
    Rieske iron-sulfur protein
      Short name=RISP
      Short name=ISP
    Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein

Gene names

Name:

petC

Organism

Chlamydomonas reinhardtii

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

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Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.
Catalytic activity	Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin.
Cofactor	Binds 1 2Fe-2S cluster per subunit.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer.
Subcellular location	Plastid › chloroplast thylakoid membrane; Single-pass membrane protein. Note= The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer.
Miscellaneous	This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus. The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

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Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW thylakoid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol-plastocyanin reductase activity Inferred from electronic annotation. Source: EC ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 29

Chloroplast

Chain

30 – 206

177

Cytochrome b6-f complex iron-sulfur subunit, chloroplastic

PRO_0000030685

Regions

Transmembrane

39 – 68

Domain

92 – 188

Rieske

Compositional bias

29 – 32

Poly-Ala

Compositional bias

74 – 79

Poly-Gly

Sites

Metal binding

134

Iron-sulfur (2Fe-2S)

Metal binding

136

Iron-sulfur (2Fe-2S); via pros nitrogen

Metal binding

152

Iron-sulfur (2Fe-2S)

Metal binding

155

Iron-sulfur (2Fe-2S); via pros nitrogen

Amino acid modifications

Disulfide bond

139 ↔ 154

Secondary structure

206

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P49728-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 7B2C2118E9F75974
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FASTA

206

21,526

        10         20         30         40         50         60 
MAMLSSRRVA APAKASAIRR SRVMPVVRAA AASSEVPDMN KRNIMNLILA GGAGLPITTL 

        70         80         90        100        110        120 
ALGYGAFFVP PSSGGGGGGQ AAKDALGNDI KAGEWLKTHL AGDRSLSQGL KGDPTYLIVT 

       130        140        150        160        170        180 
ADSTIEKYGL NAVCTHLGCV VPWVAAENKF KCPCHGSQYN AEGKVVRGPA PLSLALAHCD 

       190        200 
VAESGLVTFS TWTETDFRTG LEPWWA

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References

[1]	"Characterization of the gene of the chloroplast Rieske iron-sulfur protein in Chlamydomonas reinhardtii. Indications for an uncleaved lumen targeting sequence." de Vitry C. J. Biol. Chem. 269:7603-7609(1994) [PubMed: 8125984] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cw15.
[2]	"A cDNA encoding chloroplast Rieske protein from Chlamydomonas reinhardtii." Takahashi Y., Kataoka S., Matsubara H., Malkin R. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 2137.
[3]	"Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii." Pierre Y., Breyton C., Kramer D., Popot J.-L. J. Biol. Chem. 270:29342-29349(1995) [PubMed: 7493968] [Abstract] Cited for: PROTEIN SEQUENCE OF 30-56 AND 168-186, CHARACTERIZATION. Strain: WT12.
[4]	"An atypical haem in the cytochrome b(6)f complex." Stroebel D., Choquet Y., Popot J.-L., Picot D. Nature 426:413-418(2003) [PubMed: 14647374] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 31-206.

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Cross-references

Sequence databases

X76299 Genomic DNA. Translation: CAA53947.1.
D32003 mRNA. Translation: BAA22147.1.

PIR

A53412.

RefSeq

XP_001698786.1.

UniGene

Cre.13203

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Q90	X-ray	3.10	C	80-206	[»]
			R	31-79	[»]

ModBase

Search...

Genome annotation databases

GeneID

5724296.

KEGG

cre:CHLREDRAFT_193296.

Family and domain databases

InterPro

IPR014909. Cyt_b6-F_Fe-S_su.
IPR014349. Rieske.
IPR005805. Rieske_C.
IPR005806. Rieske_reg.
[Graphical view]

Gene3D

G3DSA:2.102.10.10. Rieske_reg. 1 hit.

PANTHER

PTHR10134. Rieske. 1 hit.

Pfam

PF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]

PRINTS

PR00162. RIESKE.

PROSITE

PS51296. RIESKE. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

UCRIA_CHLRE

Accession

Primary (citable) accession number: P49728

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Family and domain databases

Entry information

Relevant documents