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P49728

- UCRIA_CHLRE

UniProt

P49728 - UCRIA_CHLRE

Protein

Cytochrome b6-f complex iron-sulfur subunit, chloroplastic

Gene

petC

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

    Catalytic activityi

    Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 4 H+(Side 2).

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi134 – 1341Iron-sulfur (2Fe-2S)
    Metal bindingi136 – 1361Iron-sulfur (2Fe-2S); via pros nitrogen
    Metal bindingi152 – 1521Iron-sulfur (2Fe-2S)
    Metal bindingi155 – 1551Iron-sulfur (2Fe-2S); via pros nitrogen

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. plastoquinol--plastocyanin reductase activity Source: UniProtKB-EC
    4. ubiquinol-cytochrome-c reductase activity Source: InterPro

    GO - Biological processi

    1. defense response to bacterium Source: EnsemblPlants/Gramene
    2. nonphotochemical quenching Source: EnsemblPlants/Gramene
    3. response to karrikin Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:CHLREDRAFT_193296-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (EC:1.10.9.1)
    Alternative name(s):
    Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
    Rieske iron-sulfur protein
    Short name:
    ISP
    Short name:
    RISP
    Gene namesi
    Name:petC
    OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
    Taxonomic identifieri3055 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

    Subcellular locationi

    Plastidchloroplast thylakoid membrane; Single-pass membrane protein
    Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer.

    GO - Cellular componenti

    1. chloroplast envelope Source: EnsemblPlants/Gramene
    2. chloroplast thylakoid membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Thylakoid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2929Chloroplast1 PublicationAdd
    BLAST
    Chaini30 – 206177Cytochrome b6-f complex iron-sulfur subunit, chloroplasticPRO_0000030685Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi139 ↔ 154

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiP49728.

    Interactioni

    Subunit structurei

    The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer.

    Protein-protein interaction databases

    DIPiDIP-58594N.

    Structurei

    Secondary structure

    1
    206
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi39 – 6830
    Helixi92 – 987
    Helixi110 – 1123
    Beta strandi114 – 1196
    Beta strandi123 – 1253
    Beta strandi127 – 1315
    Turni135 – 1373
    Turni145 – 1484
    Beta strandi149 – 1513
    Turni153 – 1553
    Beta strandi158 – 1603
    Beta strandi165 – 1695
    Beta strandi176 – 1805
    Beta strandi188 – 1914
    Turni197 – 1993

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Q90X-ray3.10C80-206[»]
    R31-79[»]
    ProteinModelPortaliP49728.
    SMRiP49728. Positions 33-71, 80-206.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP49728.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei39 – 6830HelicalAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini92 – 18897RieskePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi29 – 324Poly-Ala
    Compositional biasi74 – 796Poly-Gly

    Sequence similaritiesi

    Contains 1 Rieske domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0723.
    KOiK02636.
    OMAiPWTETDF.

    Family and domain databases

    Gene3Di2.102.10.10. 1 hit.
    InterProiIPR014909. Cyt_b6-f_cplx_Fe-S_su.
    IPR017941. Rieske_2Fe-2S.
    IPR014349. Rieske_Fe-S_prot.
    IPR005805. Rieske_Fe-S_prot_C.
    [Graphical view]
    PANTHERiPTHR10134. PTHR10134. 1 hit.
    PfamiPF08802. CytB6-F_Fe-S. 1 hit.
    PF00355. Rieske. 1 hit.
    [Graphical view]
    PRINTSiPR00162. RIESKE.
    SUPFAMiSSF50022. SSF50022. 1 hit.
    PROSITEiPS51296. RIESKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49728-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMLSSRRVA APAKASAIRR SRVMPVVRAA AASSEVPDMN KRNIMNLILA    50
    GGAGLPITTL ALGYGAFFVP PSSGGGGGGQ AAKDALGNDI KAGEWLKTHL 100
    AGDRSLSQGL KGDPTYLIVT ADSTIEKYGL NAVCTHLGCV VPWVAAENKF 150
    KCPCHGSQYN AEGKVVRGPA PLSLALAHCD VAESGLVTFS TWTETDFRTG 200
    LEPWWA 206
    Length:206
    Mass (Da):21,526
    Last modified:October 1, 1996 - v1
    Checksum:i7B2C2118E9F75974
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76299 Genomic DNA. Translation: CAA53947.1.
    D32003 mRNA. Translation: BAA22147.1.
    PIRiA53412.
    RefSeqiXP_001698786.1. XM_001698734.1.
    UniGeneiCre.13203.

    Genome annotation databases

    GeneIDi5724296.
    KEGGicre:CHLREDRAFT_193296.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76299 Genomic DNA. Translation: CAA53947.1 .
    D32003 mRNA. Translation: BAA22147.1 .
    PIRi A53412.
    RefSeqi XP_001698786.1. XM_001698734.1.
    UniGenei Cre.13203.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Q90 X-ray 3.10 C 80-206 [» ]
    R 31-79 [» ]
    ProteinModelPortali P49728.
    SMRi P49728. Positions 33-71, 80-206.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-58594N.

    Proteomic databases

    PRIDEi P49728.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5724296.
    KEGGi cre:CHLREDRAFT_193296.

    Phylogenomic databases

    eggNOGi COG0723.
    KOi K02636.
    OMAi PWTETDF.

    Enzyme and pathway databases

    BioCyci MetaCyc:CHLREDRAFT_193296-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P49728.

    Family and domain databases

    Gene3Di 2.102.10.10. 1 hit.
    InterProi IPR014909. Cyt_b6-f_cplx_Fe-S_su.
    IPR017941. Rieske_2Fe-2S.
    IPR014349. Rieske_Fe-S_prot.
    IPR005805. Rieske_Fe-S_prot_C.
    [Graphical view ]
    PANTHERi PTHR10134. PTHR10134. 1 hit.
    Pfami PF08802. CytB6-F_Fe-S. 1 hit.
    PF00355. Rieske. 1 hit.
    [Graphical view ]
    PRINTSi PR00162. RIESKE.
    SUPFAMi SSF50022. SSF50022. 1 hit.
    PROSITEi PS51296. RIESKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the gene of the chloroplast Rieske iron-sulfur protein in Chlamydomonas reinhardtii. Indications for an uncleaved lumen targeting sequence."
      de Vitry C.
      J. Biol. Chem. 269:7603-7609(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cw15.
    2. "A cDNA encoding chloroplast Rieske protein from Chlamydomonas reinhardtii."
      Takahashi Y., Kataoka S., Matsubara H., Malkin R.
      Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: 2137.
    3. "Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii."
      Pierre Y., Breyton C., Kramer D., Popot J.-L.
      J. Biol. Chem. 270:29342-29349(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 30-56 AND 168-186, CHARACTERIZATION.
      Strain: WT12.
    4. "An atypical haem in the cytochrome b(6)f complex."
      Stroebel D., Choquet Y., Popot J.-L., Picot D.
      Nature 426:413-418(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 31-206.

    Entry informationi

    Entry nameiUCRIA_CHLRE
    AccessioniPrimary (citable) accession number: P49728
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus.
    The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3