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P49728 (UCRIA_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome b6-f complex iron-sulfur subunit, chloroplastic

EC=1.10.9.1
Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Rieske iron-sulfur protein
Short name=ISP
Short name=RISP
Gene names
Name:petC
OrganismChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifier3055 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Protein attributes

Sequence length206 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

Catalytic activity

Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 4 H+(Side 2).

Cofactor

Binds 1 2Fe-2S cluster per subunit.

Subunit structure

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer.

Subcellular location

Plastidchloroplast thylakoid membrane; Single-pass membrane protein. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer.

Miscellaneous

This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus.

The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.

Sequence similarities

Contains 1 Rieske domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2929Chloroplast Ref.3
Chain30 – 206177Cytochrome b6-f complex iron-sulfur subunit, chloroplastic
PRO_0000030685

Regions

Transmembrane39 – 6830Helical
Domain92 – 18897Rieske
Compositional bias29 – 324Poly-Ala
Compositional bias74 – 796Poly-Gly

Sites

Metal binding1341Iron-sulfur (2Fe-2S)
Metal binding1361Iron-sulfur (2Fe-2S); via pros nitrogen
Metal binding1521Iron-sulfur (2Fe-2S)
Metal binding1551Iron-sulfur (2Fe-2S); via pros nitrogen

Amino acid modifications

Disulfide bond139 ↔ 154

Secondary structure

.............................. 206
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P49728 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 7B2C2118E9F75974

FASTA20621,526
        10         20         30         40         50         60 
MAMLSSRRVA APAKASAIRR SRVMPVVRAA AASSEVPDMN KRNIMNLILA GGAGLPITTL 

        70         80         90        100        110        120 
ALGYGAFFVP PSSGGGGGGQ AAKDALGNDI KAGEWLKTHL AGDRSLSQGL KGDPTYLIVT 

       130        140        150        160        170        180 
ADSTIEKYGL NAVCTHLGCV VPWVAAENKF KCPCHGSQYN AEGKVVRGPA PLSLALAHCD 

       190        200 
VAESGLVTFS TWTETDFRTG LEPWWA 

« Hide

References

[1]"Characterization of the gene of the chloroplast Rieske iron-sulfur protein in Chlamydomonas reinhardtii. Indications for an uncleaved lumen targeting sequence."
de Vitry C.
J. Biol. Chem. 269:7603-7609(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cw15.
[2]"A cDNA encoding chloroplast Rieske protein from Chlamydomonas reinhardtii."
Takahashi Y., Kataoka S., Matsubara H., Malkin R.
Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 2137.
[3]"Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii."
Pierre Y., Breyton C., Kramer D., Popot J.-L.
J. Biol. Chem. 270:29342-29349(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 30-56 AND 168-186, CHARACTERIZATION.
Strain: WT12.
[4]"An atypical haem in the cytochrome b(6)f complex."
Stroebel D., Choquet Y., Popot J.-L., Picot D.
Nature 426:413-418(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 31-206.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X76299 Genomic DNA. Translation: CAA53947.1.
D32003 mRNA. Translation: BAA22147.1.
PIRA53412.
RefSeqXP_001698786.1. XM_001698734.1.
UniGeneCre.13203.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q90X-ray3.10C80-206[»]
R31-79[»]
ProteinModelPortalP49728.
SMRP49728. Positions 33-71, 80-206.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-58594N.

Proteomic databases

PRIDEP49728.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5724296.
KEGGcre:CHLREDRAFT_193296.

Phylogenomic databases

eggNOGCOG0723.
KOK02636.
OMAPWTETDF.

Enzyme and pathway databases

BioCycMetaCyc:CHLREDRAFT_193296-MONOMER.

Family and domain databases

Gene3D2.102.10.10. 1 hit.
InterProIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERPTHR10134. PTHR10134. 1 hit.
PfamPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSPR00162. RIESKE.
SUPFAMSSF50022. SSF50022. 1 hit.
PROSITEPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP49728.

Entry information

Entry nameUCRIA_CHLRE
AccessionPrimary (citable) accession number: P49728
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references