Reviewed, UniProtKB/Swiss-Prot P49727 (UCRI_MAIZE)

Names and origin

Protein names	Recommended name:     Cytochrome b-c1 complex subunit Rieske, mitochondrial     EC=1.10.2.2 Alternative name(s):     Ubiquinol-cytochrome c reductase iron-sulfur subunit     Rieske iron-sulfur protein       Short name=RISP     Complex III subunit 5
Organism	Zea mays (Maize)
Taxonomic identifier	4577 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The bc1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 61	61	Mitochondrion Potential
	Chain	62 – 273	212	Cytochrome b-c1 complex subunit Rieske, mitochondrial		PRO_0000030674
Regions
	Domain	176 – 271	96	Rieske
Sites
	Metal binding	216	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	218	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	235	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	238	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	221 ↔ 237		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49727-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 43B08C116E9B1A2C	FASTA	273	29,835
	10 20 30 40 50 60 MLRVAGRRLS SSLSWRPAAA VARGPLAGAG VPDRDDDSAR GRSQPRFSID SPFFVASRGF 70 80 90 100 110 120 SSTETVVPRN QDAGLADLPA TVAAVKNPNP KVVYDEYNHE RYPPGDPSKR AFAYFVLSGG 130 140 150 160 170 180 RFIYASLLRL LVLKFVLSMS ASKDVLALAS LEVDLSSIEP GTTVTVKWRG KPVFIRRRTE 190 200 210 220 230 240 DDIKLANSVD VASLRHPEQD AERVKNPEWL VVIGVCTHLG CIPLPNAGDF GGWFCPCHGS 250 260 270 HYDISGRIRK GPAPFNLEVP TYSFLEENKL LVG

References

[1]	"Functional analysis in yeast of cDNA coding for the mitochondrial Rieske iron-sulfur protein of higher plants." Huang J.T., Struck F., Matzinger D.F., Levings C.S. III Proc. Natl. Acad. Sci. U.S.A. 88:10716-10720(1991) [PubMed: 1961737] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M77224 mRNA. Translation: AAA33507.1.
PIR	A41607.
RefSeq	NP_001105561.1.
UniGene	Zm.94056
3D structure databases
HSSP	HSSP built from PDB template 1RIE based on UniProtKB P13272.
ModBase	Search...
Genome annotation databases
GeneID	542550.
Organism-specific databases
Gramene	P49727.
MaizeGDB	30151.
Enzyme and pathway databases
BRENDA	1.10.2.2. 289.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. IPR004192. Ubiquinol_cyt_Rdtase_TM. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF02921. UCR_TM. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_MAIZE

Accession

Primary (citable) accession number: P49727

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	July 28, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)