UniProtKB - P49723 (RIR4_YEAST)
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Protein
Ribonucleoside-diphosphate reductase small chain 2
Gene
RNR4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. RNR4 is required for proper folding of RNR2 and assembly with the large subunits.1 Publication
Miscellaneous
Present with 88884 molecules/cell in log phase SD medium.1 Publication
Lacks 3 iron-binding residues conserved in all other R2 subunits.
Catalytic activityi
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation
Kineticsi
- Vmax=2250 nmol/min/mg enzyme for cytidine 5'-diphosphate1 Publication
Temperature dependencei
Optimum temperature is 30 degrees Celsius.1 Publication
: DNA replication Pathwayi
This protein is involved in the pathway DNA replication, which is part of Genetic information processing.View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 131 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- protein heterodimerization activity Source: CAFA
- ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: SGD
GO - Biological processi
- cofactor biosynthetic process Source: SGD
- deoxyribonucleotide biosynthetic process Source: SGD
- DNA replication Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | DNA replication |
Enzyme and pathway databases
BioCyci | MetaCyc:YGR180C-MONOMER. YEAST:YGR180C-MONOMER. |
UniPathwayi | UPA00326. |
Names & Taxonomyi
Protein namesi | Recommended name: Ribonucleoside-diphosphate reductase small chain 2 (EC:1.17.4.1)Alternative name(s): Ribonucleotide reductase R2 subunit 2 Ribonucleotide reductase small subunit 2 |
Gene namesi | Name:RNR4 Synonyms:CRT3 Ordered Locus Names:YGR180C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YGR180C. |
SGDi | S000003412. RNR4. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000190465 | 1 – 345 | Ribonucleoside-diphosphate reductase small chain 2Add BLAST | 345 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources1 Publication | 1 | |
Modified residuei | 169 | PhosphoserineCombined sources | 1 | |
Modified residuei | 332 | PhosphoserineCombined sources | 1 | |
Modified residuei | 334 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 336 | PhosphoserineCombined sources | 1 | |
Cross-linki | 337 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P49723. |
PaxDbi | P49723. |
PRIDEi | P49723. |
TopDownProteomicsi | P49723. |
PTM databases
iPTMneti | P49723. |
Expressioni
Inductioni
Induced by DNA-damage.1 Publication
Interactioni
Subunit structurei
Heterotetramer of two large (R1) and two small (R2) subunits. S.cerevisiae has two different R1 subunits (RNR1 and RNR3) and two different R2 subunits (RNR2 and RNR4). The functional form of the small subunits is a RNR2-RNR4 heterodimer, where RNR2 provides the iron-radical center and RNR4 is required for proper folding of RNR2 and assembly with the large subunits. Under normal growth conditions, the active form of the large subunits is a homodimer of the constitutively expressed RNR1. In damaged cells or cells arrested for DNA synthesis, the reductase consists of multiple species because of the association of the small subunits (RNR2-RNR4) with either the RNR1 homodimer or a heterodimer of RNR1 and the damage-inducible RNR3. Interacts with DIF1.3 Publications
Binary interactionsi
GO - Molecular functioni
- protein heterodimerization activity Source: CAFA
Protein-protein interaction databases
BioGridi | 33432. 193 interactors. |
DIPi | DIP-5381N. |
IntActi | P49723. 38 interactors. |
MINTi | P49723. |
STRINGi | 4932.YGR180C. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 4 – 9 | Combined sources | 6 | |
Helixi | 13 – 21 | Combined sources | 9 | |
Turni | 22 – 24 | Combined sources | 3 | |
Turni | 26 – 28 | Combined sources | 3 | |
Helixi | 43 – 54 | Combined sources | 12 | |
Helixi | 59 – 61 | Combined sources | 3 | |
Turni | 65 – 67 | Combined sources | 3 | |
Turni | 69 – 73 | Combined sources | 5 | |
Helixi | 78 – 89 | Combined sources | 12 | |
Helixi | 101 – 107 | Combined sources | 7 | |
Helixi | 111 – 138 | Combined sources | 28 | |
Helixi | 142 – 144 | Combined sources | 3 | |
Helixi | 148 – 151 | Combined sources | 4 | |
Helixi | 155 – 166 | Combined sources | 12 | |
Beta strandi | 169 – 172 | Combined sources | 4 | |
Helixi | 175 – 187 | Combined sources | 13 | |
Turni | 188 – 190 | Combined sources | 3 | |
Helixi | 191 – 200 | Combined sources | 10 | |
Beta strandi | 202 – 205 | Combined sources | 4 | |
Helixi | 207 – 232 | Combined sources | 26 | |
Helixi | 240 – 258 | Combined sources | 19 | |
Turni | 265 – 268 | Combined sources | 4 | |
Helixi | 277 – 288 | Combined sources | 12 | |
Helixi | 316 – 322 | Combined sources | 7 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JK0 | X-ray | 2.80 | B | 1-345 | [»] | |
1SMS | X-ray | 3.10 | A/B | 1-345 | [»] | |
1ZZD | X-ray | 2.60 | B | 337-345 | [»] | |
DisProti | DP00488. | |||||
ProteinModelPortali | P49723. | |||||
SMRi | P49723. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P49723. |
Family & Domainsi
Sequence similaritiesi
Belongs to the ribonucleoside diphosphate reductase small chain family.Curated
Phylogenomic databases
HOGENOMi | HOG000255975. |
InParanoidi | P49723. |
KOi | K10808. |
OrthoDBi | EOG092C3G06. |
Family and domain databases
CDDi | cd01049. RNRR2. 1 hit. |
Gene3Di | 1.10.620.20. 1 hit. |
InterProi | View protein in InterPro IPR009078. Ferritin-like_SF. IPR012348. RNR-like. IPR033909. RNR_small. IPR030475. RNR_small_AS. IPR000358. RNR_small_fam. |
PANTHERi | PTHR23409. PTHR23409. 1 hit. |
Pfami | View protein in Pfam PF00268. Ribonuc_red_sm. 1 hit. |
SUPFAMi | SSF47240. SSF47240. 1 hit. |
PROSITEi | View protein in PROSITE PS00368. RIBORED_SMALL. 1 hit. |
i Sequence
Sequence statusi: Complete.
P49723-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEAHNQFLKT FQKERHDMKE AEKDEILLME NSRRFVMFPI KYHEIWAAYK
60 70 80 90 100
KVEASFWTAE EIELAKDTED FQKLTDDQKT YIGNLLALSI SSDNLVNKYL
110 120 130 140 150
IENFSAQLQN PEGKSFYGFQ IMMENIYSEV YSMMVDAFFK DPKNIPLFKE
160 170 180 190 200
IANLPEVKHK AAFIERWISN DDSLYAERLV AFAAKEGIFQ AGNYASMFWL
210 220 230 240 250
TDKKIMPGLA MANRNICRDR GAYTDFSCLL FAHLRTKPNP KIIEKIITEA
260 270 280 290 300
VEIEKEYYSN SLPVEKFGMD LKSIHTYIEF VADGLLQGFG NEKYYNAVNP
310 320 330 340
FEFMEDVATA GKTTFFEKKV SDYQKASDMS KSATPSKEIN FDDDF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 127 | Y → H in AAU09736 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U30385 Genomic DNA. Translation: AAB72236.1. Z72965 Genomic DNA. Translation: CAA97206.1. AY723819 Genomic DNA. Translation: AAU09736.1. BK006941 Genomic DNA. Translation: DAA08275.1. |
PIRi | S59744. |
RefSeqi | NP_011696.3. NM_001181309.3. |
Genome annotation databases
EnsemblFungii | YGR180C; YGR180C; YGR180C. |
GeneIDi | 853091. |
KEGGi | sce:YGR180C. |
Similar proteinsi
Entry informationi
Entry namei | RIR4_YEAST | |
Accessioni | P49723Primary (citable) accession number: P49723 Secondary accession number(s): D6VUW4, Q66R92 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | March 28, 2018 | |
This is version 184 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |