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Protein

DNA replication licensing factor MCM5

Gene

Mcm5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi380 – 3878ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM5 (EC:3.6.4.12)
Alternative name(s):
CDC46 homolog
P1-CDC46
Gene namesi
Name:Mcm5
Synonyms:Cdc46, Mcmd5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:103197. Mcm5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 733732DNA replication licensing factor MCM5PRO_0000194108Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei391 – 3911N6-acetyllysineBy similarity
Modified residuei395 – 3951N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP49718.
MaxQBiP49718.
PaxDbiP49718.
PRIDEiP49718.

PTM databases

iPTMnetiP49718.
PhosphoSiteiP49718.
SwissPalmiP49718.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with MCMBP (By simililarity). Interacts with ANKRD17.By similarity

Protein-protein interaction databases

DIPiDIP-45875N.
IntActiP49718. 1 interaction.
STRINGi10090.ENSMUSP00000126135.

Structurei

3D structure databases

ProteinModelPortaliP49718.
SMRiP49718. Positions 4-684.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini330 – 536207MCMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi511 – 5144Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiKOG0481. Eukaryota.
COG1241. LUCA.
HOVERGENiHBG104907.
InParanoidiP49718.
PhylomeDBiP49718.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008048. MCM5.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01661. MCMPROTEIN5.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49718-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGFDDPGIF YSDSFGGDPG AEEGQARKSQ LQRRFKEFLR QYRVGTDRTG
60 70 80 90 100
FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEELADHLHK QPAEHLQLLE
110 120 130 140 150
EAAKEVADEV TRPRPAGDEL LQDIQVMLKS DASPSSIRIL KSDMMSHLVK
160 170 180 190 200
IPGIIISASA VRAKATRISI QCRSCHNTLT NIAMPRPRGL CLPRKCNMDQ
210 220 230 240 250
AGRPKCPLDP YFIMPDKCKC VDFQTLKLQE LPDAVPHGEM PRHMQLYCDR
260 270 280 290 300
YLCDKVVPGN RVTIMGIYSI KKFGLNPSKG RDRVGVGIRS SYIRVLGIQV
310 320 330 340 350
DTDGSGRSFA GSVSPQEEEE FRRLAALPNI YELISKSISP SIFGGMDMKK
360 370 380 390 400
AIACLLFGGS RKRLPDGLTR RGDINLLMLG DPGTAKSQLL KFVEKCSPIG
410 420 430 440 450
VYTSGKGSSA AGLTASVIRD PSSRNFIMEG GAMVLADGGV VCIDEFDKMR
460 470 480 490 500
EDDRVAIHEA MEQQTISIAK AGITTTLNSR CSVLAAANSV FGRWDETKGE
510 520 530 540 550
DNIDFMPTIL SRFDMIFIVK DEHNEERDMM LAKHVMTLHV SALTQTQAVE
560 570 580 590 600
GEIDLAKMKK FIAYCRARCG PRLSAEAAEK LKNRYIIMRS GARQHERDSD
610 620 630 640 650
RRSSIPITVR QLEAIVRIAE ALSKMKLQPF ATEADVEEAL RLFQVSTLDA
660 670 680 690 700
ALSGNLSGVE GFTTQEDQEM LSRIEKQLKR RFAIGSQVSE HSIVQDFTKQ
710 720 730
KYPEHAIRKV LQLMLRRGEI QHRMQRKVLY RLK
Length:733
Mass (Da):82,343
Last modified:October 1, 1996 - v1
Checksum:iF1047AE93A4439BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26090 mRNA. Translation: BAA05083.1.
PIRiS56767.
UniGeneiMm.5048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26090 mRNA. Translation: BAA05083.1.
PIRiS56767.
UniGeneiMm.5048.

3D structure databases

ProteinModelPortaliP49718.
SMRiP49718. Positions 4-684.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-45875N.
IntActiP49718. 1 interaction.
STRINGi10090.ENSMUSP00000126135.

PTM databases

iPTMnetiP49718.
PhosphoSiteiP49718.
SwissPalmiP49718.

Proteomic databases

EPDiP49718.
MaxQBiP49718.
PaxDbiP49718.
PRIDEiP49718.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:103197. Mcm5.

Phylogenomic databases

eggNOGiKOG0481. Eukaryota.
COG1241. LUCA.
HOVERGENiHBG104907.
InParanoidiP49718.
PhylomeDBiP49718.

Miscellaneous databases

ChiTaRSiMcm5. mouse.
PROiP49718.
SOURCEiSearch...

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008048. MCM5.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01661. MCMPROTEIN5.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of cDNA encoding mouse Cdc21 and CDC46 homologs and characterization of the products: physical interaction between P1(MCM3) and CDC46 proteins."
    Kimura H., Takizawa N., Nozaki N., Sugimoto K.
    Nucleic Acids Res. 23:2097-2104(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiMCM5_MOUSE
AccessioniPrimary (citable) accession number: P49718
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 16, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.