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Protein

DNA replication licensing factor MCM5

Gene

Mcm5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi380 – 387ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM5 (EC:3.6.4.12)
Alternative name(s):
CDC46 homolog
P1-CDC46
Gene namesi
Name:Mcm5
Synonyms:Cdc46, Mcmd5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:103197 Mcm5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001941082 – 733DNA replication licensing factor MCM5Add BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei314PhosphoserineBy similarity1
Modified residuei391N6-acetyllysineBy similarity1
Modified residuei395N6-acetyllysineBy similarity1
Modified residuei604PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP49718
MaxQBiP49718
PaxDbiP49718
PeptideAtlasiP49718
PRIDEiP49718

PTM databases

iPTMnetiP49718
PhosphoSitePlusiP49718
SwissPalmiP49718

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with MCMBP (By simililarity). Interacts with ANKRD17.By similarity

Protein-protein interaction databases

CORUMiP49718
DIPiDIP-45875N
IntActiP49718, 5 interactors
STRINGi10090.ENSMUSP00000126135

Structurei

3D structure databases

ProteinModelPortaliP49718
SMRiP49718
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini330 – 536MCMAdd BLAST207

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi511 – 514Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGiKOG0481 Eukaryota
COG1241 LUCA
HOVERGENiHBG104907
InParanoidiP49718
PhylomeDBiP49718

Family and domain databases

InterProiView protein in InterPro
IPR031327 MCM
IPR008048 MCM5
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01661 MCMPROTEIN5
SMARTiView protein in SMART
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49718-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGFDDPGIF YSDSFGGDPG AEEGQARKSQ LQRRFKEFLR QYRVGTDRTG
60 70 80 90 100
FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEELADHLHK QPAEHLQLLE
110 120 130 140 150
EAAKEVADEV TRPRPAGDEL LQDIQVMLKS DASPSSIRIL KSDMMSHLVK
160 170 180 190 200
IPGIIISASA VRAKATRISI QCRSCHNTLT NIAMPRPRGL CLPRKCNMDQ
210 220 230 240 250
AGRPKCPLDP YFIMPDKCKC VDFQTLKLQE LPDAVPHGEM PRHMQLYCDR
260 270 280 290 300
YLCDKVVPGN RVTIMGIYSI KKFGLNPSKG RDRVGVGIRS SYIRVLGIQV
310 320 330 340 350
DTDGSGRSFA GSVSPQEEEE FRRLAALPNI YELISKSISP SIFGGMDMKK
360 370 380 390 400
AIACLLFGGS RKRLPDGLTR RGDINLLMLG DPGTAKSQLL KFVEKCSPIG
410 420 430 440 450
VYTSGKGSSA AGLTASVIRD PSSRNFIMEG GAMVLADGGV VCIDEFDKMR
460 470 480 490 500
EDDRVAIHEA MEQQTISIAK AGITTTLNSR CSVLAAANSV FGRWDETKGE
510 520 530 540 550
DNIDFMPTIL SRFDMIFIVK DEHNEERDMM LAKHVMTLHV SALTQTQAVE
560 570 580 590 600
GEIDLAKMKK FIAYCRARCG PRLSAEAAEK LKNRYIIMRS GARQHERDSD
610 620 630 640 650
RRSSIPITVR QLEAIVRIAE ALSKMKLQPF ATEADVEEAL RLFQVSTLDA
660 670 680 690 700
ALSGNLSGVE GFTTQEDQEM LSRIEKQLKR RFAIGSQVSE HSIVQDFTKQ
710 720 730
KYPEHAIRKV LQLMLRRGEI QHRMQRKVLY RLK
Length:733
Mass (Da):82,343
Last modified:October 1, 1996 - v1
Checksum:iF1047AE93A4439BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26090 mRNA Translation: BAA05083.1
PIRiS56767
UniGeneiMm.5048

Similar proteinsi

Entry informationi

Entry nameiMCM5_MOUSE
AccessioniPrimary (citable) accession number: P49718
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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