DNA replication licensing factor MCM4

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P49717 (MCM4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA replication licensing factor MCM4
EC=3.6.4.12

Alternative name(s):
CDC21 homolog
P1-CDC21

Gene names

Name:	Mcm4
Synonyms:	Cdc21, Mcmd4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	862 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity By similarity.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Subunit structure	Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with MCMBP (By simililarity).
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the MCM family. Contains 1 MCM domain.

Ontologies

Keywords
Biological process	Cell cycle DNA replication
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication initiation Inferred from electronic annotation. Source: InterPro DNA unwinding involved in replication Inferred from physical interaction PubMed 15917436. Source: MGI cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	MCM complex Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent DNA helicase activity Inferred from electronic annotation. Source: Compara single-stranded DNA binding Inferred from physical interaction PubMed 15917436. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 862

861

DNA replication licensing factor MCM4

PRO_0000194102

Regions

Domain

457 – 666

210

MCM

Nucleotide binding

509 – 516

ATP Potential

Motif

641 – 644

Arginine finger

Amino acid modifications

Modified residue

N-acetylserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

119

Phosphoserine By similarity

Modified residue

130

Phosphoserine By similarity

Modified residue

141

Phosphoserine By similarity

Modified residue

144

Phosphoserine By similarity

Modified residue

219

N6-acetyllysine By similarity

Modified residue

449

N6-acetyllysine By similarity

Experimental info

Sequence conflict

530

Q → R in AAC36509. Ref.2

Sequence conflict

572

I → T in AAC36509. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P49717 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 516ACC1A3C6FB16E
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FASTA

862

96,736

        10         20         30         40         50         60 
MSSPASTPSR RSSRRGRVTP TQSLRSEESR SSPNRRRRGE DSSTGELLPM PTSPGADLQS 

        70         80         90        100        110        120 
PPAQNALFSS PPQMHSLAIP LDFDVSSPLT YGTPSSRVEG TPRSGVRGTP VRQRPDLGSA 

       130        140        150        160        170        180 
RKGLQVDLQS DGAAAEDIVP SEQSLGQKLV IWGTDVNVAT CKENFQRFLQ CFTDPLAKEE 

       190        200        210        220        230        240 
ENVGIDITQP LYMQQLGEIN ITGEPFLNVN CEHIKSFSKN LYRQLISYPQ EVIPTFDMAV 

       250        260        270        280        290        300 
NEIFFDRYPD SILEHQIQVR PFNALKTKSM RNLNPEDIDQ LITISGMVIR TSQLIPEMQE 

       310        320        330        340        350        360 
AFFQCQVCAH TTRVEIDRGR IAEPCSCVHC HTTHSMALIH NRSFFSDKQM IKLQESPEDM 

       370        380        390        400        410        420 
PAGQTPHTIV LFAHNDLVDK VQPGDRVNVT GIYRAVPIRV NPRVSNVKSV YKTHIDVIHY 

       430        440        450        460        470        480 
RKTDAKRLHG LDEEAEQKLF SEKRVKLLKE LSRKPDIYER LASALAPSIY EHEDIKKGIL 

       490        500        510        520        530        540 
LQLFGGTRKD FSHTGRGKFR AEINILLCGD PGTSKSQLLQ YVYNLVPRGQ YTSGKGSSAV 

       550        560        570        580        590        600 
GLTAYVMKDP ETRQLVLQTG ALVLSDNGIC CIDEFDKMNE STRSVLHEVM EQQTLSIAKA 

       610        620        630        640        650        660 
GIICQLNART SVLAAANPIE SQWNPKKTTI ENIQLPHTLL SRFDLIFLML DPQDEAYDRR 

       670        680        690        700        710        720 
LAHHLVSLYY QSEEQVEEEF LDMAVLKDYI AYAHSTIMPR LSEEASQALI EAYVNMRKIG 

       730        740        750        760        770        780 
SSRGMVSAYP RQLESLIRLA EAHAKVRFSN KVEAIDVEEA KRLHREALKQ SATDPRTGIV 

       790        800        810        820        830        840 
DISILTTGMS ATSRKRKEEL AEALRKLILS KGKTPALKYQ QLFEDIRGQS DTAITKDMFE 

       850        860 
EALRALADDD FLTVTGKTVR LL

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References

[1]	"Molecular cloning of cDNA encoding mouse Cdc21 and CDC46 homologs and characterization of the products: physical interaction between P1(MCM3) and CDC46 proteins." Kimura H., Takizawa N., Nozaki N., Sugimoto K. Nucleic Acids Res. 23:2097-2104(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis." Chu C.C., Paul W.E. Mol. Immunol. 35:487-502(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 503-602. Strain: BALB/c. Tissue: Spleen.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26089 mRNA. Translation: BAA05082.1. U89402 mRNA. Translation: AAC36509.1.
IPI	IPI00117016.
PIR	S56766.
RefSeq	NP_032591.3. NM_008565.3.
UniGene	Mm.1500.
3D structure databases
ProteinModelPortal	P49717.
SMR	P49717. Positions 189-765.
ModBase	Search...
PTM databases
PhosphoSite	P49717.
Proteomic databases
PaxDb	P49717.
PRIDE	P49717.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023353; ENSMUSP00000023353; ENSMUSG00000022673.
GeneID	17217.
KEGG	mmu:17217.
Organism-specific databases
CTD	4173.
MGI	MGI:103199. Mcm4.
Phylogenomic databases
eggNOG	COG1241.
HOGENOM	HOG000224127.
HOVERGEN	HBG102781.
InParanoid	P49717.
KO	K02212.
OMA	VEMDRGR.
OrthoDB	EOG43XV2T.
Gene expression databases
ArrayExpress	P49717.
Bgee	P49717.
Genevestigator	P49717.
GermOnline	ENSMUSG00000022673. Mus musculus.
Family and domain databases
Gene3D	2.20.28.10. 1 hit. 2.40.50.140. 2 hits.
InterPro	IPR003593. AAA+_ATPase. IPR008047. MCM_4. IPR018525. MCM_CS. IPR001208. MCM_DNA-dep_ATPase. IPR012340. NA-bd_OB-fold. IPR004039. Rubredoxin-type_fold. [Graphical view]
Pfam	PF00493. MCM. 1 hit. [Graphical view]
PRINTS	PR01657. MCMFAMILY. PR01660. MCMPROTEIN4.
SMART	SM00382. AAA. 1 hit. SM00350. MCM. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
PROSITE	PS00847. MCM_1. 1 hit. PS50051. MCM_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	291610.
SOURCE	Search...

Entry information

Entry name

MCM4_MOUSE

Accession

Primary (citable) accession number: P49717
Secondary accession number(s): O89056

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents