Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P49717

- MCM4_MOUSE

UniProt

P49717 - MCM4_MOUSE

Protein

DNA replication licensing factor MCM4

Gene

Mcm4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi509 – 5168ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent DNA helicase activity Source: Ensembl
    3. protein binding Source: MGI
    4. single-stranded DNA binding Source: MGI

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. DNA replication initiation Source: InterPro
    3. DNA unwinding involved in DNA replication Source: MGI

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    Cell cycle, DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_217374. Unwinding of DNA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA replication licensing factor MCM4 (EC:3.6.4.12)
    Alternative name(s):
    CDC21 homolog
    P1-CDC21
    Gene namesi
    Name:Mcm4
    Synonyms:Cdc21, Mcmd4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:103199. Mcm4.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. MCM complex Source: UniProtKB
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 862861DNA replication licensing factor MCM4PRO_0000194102Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei26 – 261PhosphoserineBy similarity
    Modified residuei31 – 311PhosphoserineBy similarity
    Modified residuei32 – 321PhosphoserineBy similarity
    Modified residuei119 – 1191PhosphoserineBy similarity
    Modified residuei130 – 1301PhosphoserineBy similarity
    Modified residuei141 – 1411PhosphoserineBy similarity
    Modified residuei144 – 1441PhosphoserineBy similarity
    Modified residuei219 – 2191N6-acetyllysineBy similarity
    Modified residuei449 – 4491N6-acetyllysineBy similarity
    Modified residuei857 – 8571N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP49717.
    PaxDbiP49717.
    PRIDEiP49717.

    PTM databases

    PhosphoSiteiP49717.

    Expressioni

    Gene expression databases

    ArrayExpressiP49717.
    BgeeiP49717.
    GenevestigatoriP49717.

    Interactioni

    Subunit structurei

    Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with MCMBP (By simililarity).

    Protein-protein interaction databases

    BioGridi201347. 3 interactions.
    IntActiP49717. 2 interactions.
    MINTiMINT-1867081.

    Structurei

    3D structure databases

    ProteinModelPortaliP49717.
    SMRiP49717. Positions 158-765.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini457 – 666210MCMAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi641 – 6444Arginine finger

    Sequence similaritiesi

    Belongs to the MCM family.Curated
    Contains 1 MCM domain.Curated

    Phylogenomic databases

    eggNOGiCOG1241.
    HOGENOMiHOG000224127.
    HOVERGENiHBG102781.
    InParanoidiP49717.
    KOiK02212.
    OMAiMHSSAIP.
    OrthoDBiEOG78D7JF.
    PhylomeDBiP49717.
    TreeFamiTF300463.

    Family and domain databases

    Gene3Di2.20.28.10. 1 hit.
    2.40.50.140. 2 hits.
    3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR008047. MCM_4.
    IPR018525. MCM_CS.
    IPR001208. MCM_DNA-dep_ATPase.
    IPR027925. MCM_N.
    IPR012340. NA-bd_OB-fold.
    IPR027417. P-loop_NTPase.
    IPR004039. Rubredoxin-type_fold.
    [Graphical view]
    PfamiPF00493. MCM. 1 hit.
    PF14551. MCM_N. 1 hit.
    [Graphical view]
    PRINTSiPR01657. MCMFAMILY.
    PR01660. MCMPROTEIN4.
    SMARTiSM00382. AAA. 1 hit.
    SM00350. MCM. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS00847. MCM_1. 1 hit.
    PS50051. MCM_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49717-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSPASTPSR RSSRRGRVTP TQSLRSEESR SSPNRRRRGE DSSTGELLPM    50
    PTSPGADLQS PPAQNALFSS PPQMHSLAIP LDFDVSSPLT YGTPSSRVEG 100
    TPRSGVRGTP VRQRPDLGSA RKGLQVDLQS DGAAAEDIVP SEQSLGQKLV 150
    IWGTDVNVAT CKENFQRFLQ CFTDPLAKEE ENVGIDITQP LYMQQLGEIN 200
    ITGEPFLNVN CEHIKSFSKN LYRQLISYPQ EVIPTFDMAV NEIFFDRYPD 250
    SILEHQIQVR PFNALKTKSM RNLNPEDIDQ LITISGMVIR TSQLIPEMQE 300
    AFFQCQVCAH TTRVEIDRGR IAEPCSCVHC HTTHSMALIH NRSFFSDKQM 350
    IKLQESPEDM PAGQTPHTIV LFAHNDLVDK VQPGDRVNVT GIYRAVPIRV 400
    NPRVSNVKSV YKTHIDVIHY RKTDAKRLHG LDEEAEQKLF SEKRVKLLKE 450
    LSRKPDIYER LASALAPSIY EHEDIKKGIL LQLFGGTRKD FSHTGRGKFR 500
    AEINILLCGD PGTSKSQLLQ YVYNLVPRGQ YTSGKGSSAV GLTAYVMKDP 550
    ETRQLVLQTG ALVLSDNGIC CIDEFDKMNE STRSVLHEVM EQQTLSIAKA 600
    GIICQLNART SVLAAANPIE SQWNPKKTTI ENIQLPHTLL SRFDLIFLML 650
    DPQDEAYDRR LAHHLVSLYY QSEEQVEEEF LDMAVLKDYI AYAHSTIMPR 700
    LSEEASQALI EAYVNMRKIG SSRGMVSAYP RQLESLIRLA EAHAKVRFSN 750
    KVEAIDVEEA KRLHREALKQ SATDPRTGIV DISILTTGMS ATSRKRKEEL 800
    AEALRKLILS KGKTPALKYQ QLFEDIRGQS DTAITKDMFE EALRALADDD 850
    FLTVTGKTVR LL 862
    Length:862
    Mass (Da):96,736
    Last modified:October 1, 1996 - v1
    Checksum:i516ACC1A3C6FB16E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti530 – 5301Q → R in AAC36509. (PubMed:9798653)Curated
    Sequence conflicti572 – 5721I → T in AAC36509. (PubMed:9798653)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26089 mRNA. Translation: BAA05082.1.
    U89402 mRNA. Translation: AAC36509.1.
    CCDSiCCDS27977.1.
    PIRiS56766.
    RefSeqiNP_032591.3. NM_008565.3.
    UniGeneiMm.1500.

    Genome annotation databases

    EnsembliENSMUST00000023353; ENSMUSP00000023353; ENSMUSG00000022673.
    GeneIDi17217.
    KEGGimmu:17217.
    UCSCiuc007yhr.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26089 mRNA. Translation: BAA05082.1 .
    U89402 mRNA. Translation: AAC36509.1 .
    CCDSi CCDS27977.1.
    PIRi S56766.
    RefSeqi NP_032591.3. NM_008565.3.
    UniGenei Mm.1500.

    3D structure databases

    ProteinModelPortali P49717.
    SMRi P49717. Positions 158-765.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201347. 3 interactions.
    IntActi P49717. 2 interactions.
    MINTi MINT-1867081.

    PTM databases

    PhosphoSitei P49717.

    Proteomic databases

    MaxQBi P49717.
    PaxDbi P49717.
    PRIDEi P49717.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023353 ; ENSMUSP00000023353 ; ENSMUSG00000022673 .
    GeneIDi 17217.
    KEGGi mmu:17217.
    UCSCi uc007yhr.2. mouse.

    Organism-specific databases

    CTDi 4173.
    MGIi MGI:103199. Mcm4.

    Phylogenomic databases

    eggNOGi COG1241.
    HOGENOMi HOG000224127.
    HOVERGENi HBG102781.
    InParanoidi P49717.
    KOi K02212.
    OMAi MHSSAIP.
    OrthoDBi EOG78D7JF.
    PhylomeDBi P49717.
    TreeFami TF300463.

    Enzyme and pathway databases

    Reactomei REACT_217374. Unwinding of DNA.

    Miscellaneous databases

    NextBioi 291610.
    PROi P49717.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P49717.
    Bgeei P49717.
    Genevestigatori P49717.

    Family and domain databases

    Gene3Di 2.20.28.10. 1 hit.
    2.40.50.140. 2 hits.
    3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR008047. MCM_4.
    IPR018525. MCM_CS.
    IPR001208. MCM_DNA-dep_ATPase.
    IPR027925. MCM_N.
    IPR012340. NA-bd_OB-fold.
    IPR027417. P-loop_NTPase.
    IPR004039. Rubredoxin-type_fold.
    [Graphical view ]
    Pfami PF00493. MCM. 1 hit.
    PF14551. MCM_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01657. MCMFAMILY.
    PR01660. MCMPROTEIN4.
    SMARTi SM00382. AAA. 1 hit.
    SM00350. MCM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS00847. MCM_1. 1 hit.
    PS50051. MCM_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of cDNA encoding mouse Cdc21 and CDC46 homologs and characterization of the products: physical interaction between P1(MCM3) and CDC46 proteins."
      Kimura H., Takizawa N., Nozaki N., Sugimoto K.
      Nucleic Acids Res. 23:2097-2104(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Expressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis."
      Chu C.C., Paul W.E.
      Mol. Immunol. 35:487-502(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 503-602.
      Strain: BALB/c.
      Tissue: Spleen.
    3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-857, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiMCM4_MOUSE
    AccessioniPrimary (citable) accession number: P49717
    Secondary accession number(s): O89056
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3