Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P49717 (MCM4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA replication licensing factor MCM4

EC=3.6.4.12
Alternative name(s):
CDC21 homolog
P1-CDC21
Gene names
Name:Mcm4
Synonyms:Cdc21, Mcmd4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length862 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with MCMBP (By simililarity).

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the MCM family.

Contains 1 MCM domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 862861DNA replication licensing factor MCM4
PRO_0000194102

Regions

Domain457 – 666210MCM
Nucleotide binding509 – 5168ATP Potential
Motif641 – 6444Arginine finger

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue261Phosphoserine By similarity
Modified residue311Phosphoserine By similarity
Modified residue321Phosphoserine By similarity
Modified residue1191Phosphoserine By similarity
Modified residue1301Phosphoserine By similarity
Modified residue1411Phosphoserine By similarity
Modified residue1441Phosphoserine By similarity
Modified residue2191N6-acetyllysine By similarity
Modified residue4491N6-acetyllysine By similarity
Modified residue8571N6-acetyllysine Ref.3

Experimental info

Sequence conflict5301Q → R in AAC36509. Ref.2
Sequence conflict5721I → T in AAC36509. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P49717 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 516ACC1A3C6FB16E

FASTA86296,736
        10         20         30         40         50         60 
MSSPASTPSR RSSRRGRVTP TQSLRSEESR SSPNRRRRGE DSSTGELLPM PTSPGADLQS 

        70         80         90        100        110        120 
PPAQNALFSS PPQMHSLAIP LDFDVSSPLT YGTPSSRVEG TPRSGVRGTP VRQRPDLGSA 

       130        140        150        160        170        180 
RKGLQVDLQS DGAAAEDIVP SEQSLGQKLV IWGTDVNVAT CKENFQRFLQ CFTDPLAKEE 

       190        200        210        220        230        240 
ENVGIDITQP LYMQQLGEIN ITGEPFLNVN CEHIKSFSKN LYRQLISYPQ EVIPTFDMAV 

       250        260        270        280        290        300 
NEIFFDRYPD SILEHQIQVR PFNALKTKSM RNLNPEDIDQ LITISGMVIR TSQLIPEMQE 

       310        320        330        340        350        360 
AFFQCQVCAH TTRVEIDRGR IAEPCSCVHC HTTHSMALIH NRSFFSDKQM IKLQESPEDM 

       370        380        390        400        410        420 
PAGQTPHTIV LFAHNDLVDK VQPGDRVNVT GIYRAVPIRV NPRVSNVKSV YKTHIDVIHY 

       430        440        450        460        470        480 
RKTDAKRLHG LDEEAEQKLF SEKRVKLLKE LSRKPDIYER LASALAPSIY EHEDIKKGIL 

       490        500        510        520        530        540 
LQLFGGTRKD FSHTGRGKFR AEINILLCGD PGTSKSQLLQ YVYNLVPRGQ YTSGKGSSAV 

       550        560        570        580        590        600 
GLTAYVMKDP ETRQLVLQTG ALVLSDNGIC CIDEFDKMNE STRSVLHEVM EQQTLSIAKA 

       610        620        630        640        650        660 
GIICQLNART SVLAAANPIE SQWNPKKTTI ENIQLPHTLL SRFDLIFLML DPQDEAYDRR 

       670        680        690        700        710        720 
LAHHLVSLYY QSEEQVEEEF LDMAVLKDYI AYAHSTIMPR LSEEASQALI EAYVNMRKIG 

       730        740        750        760        770        780 
SSRGMVSAYP RQLESLIRLA EAHAKVRFSN KVEAIDVEEA KRLHREALKQ SATDPRTGIV 

       790        800        810        820        830        840 
DISILTTGMS ATSRKRKEEL AEALRKLILS KGKTPALKYQ QLFEDIRGQS DTAITKDMFE 

       850        860 
EALRALADDD FLTVTGKTVR LL 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of cDNA encoding mouse Cdc21 and CDC46 homologs and characterization of the products: physical interaction between P1(MCM3) and CDC46 proteins."
Kimura H., Takizawa N., Nozaki N., Sugimoto K.
Nucleic Acids Res. 23:2097-2104(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Expressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis."
Chu C.C., Paul W.E.
Mol. Immunol. 35:487-502(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 503-602.
Strain: BALB/c.
Tissue: Spleen.
[3]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-857, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D26089 mRNA. Translation: BAA05082.1.
U89402 mRNA. Translation: AAC36509.1.
PIRS56766.
RefSeqNP_032591.3. NM_008565.3.
UniGeneMm.1500.

3D structure databases

ProteinModelPortalP49717.
SMRP49717. Positions 189-765.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201347. 2 interactions.
IntActP49717. 2 interactions.
MINTMINT-1867081.

PTM databases

PhosphoSiteP49717.

Proteomic databases

PaxDbP49717.
PRIDEP49717.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023353; ENSMUSP00000023353; ENSMUSG00000022673.
GeneID17217.
KEGGmmu:17217.
UCSCuc007yhr.2. mouse.

Organism-specific databases

CTD4173.
MGIMGI:103199. Mcm4.

Phylogenomic databases

eggNOGCOG1241.
HOGENOMHOG000224127.
HOVERGENHBG102781.
InParanoidP49717.
KOK02212.
OMAMHSSAIP.
OrthoDBEOG78D7JF.
PhylomeDBP49717.
TreeFamTF300463.

Gene expression databases

ArrayExpressP49717.
BgeeP49717.
GenevestigatorP49717.

Family and domain databases

Gene3D2.20.28.10. 1 hit.
2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR008047. MCM_4.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR004039. Rubredoxin-type_fold.
[Graphical view]
PfamPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSPR01657. MCMFAMILY.
PR01660. MCMPROTEIN4.
SMARTSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio291610.
PROP49717.
SOURCESearch...

Entry information

Entry nameMCM4_MOUSE
AccessionPrimary (citable) accession number: P49717
Secondary accession number(s): O89056
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot