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P49717

- MCM4_MOUSE

UniProt

P49717 - MCM4_MOUSE

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Protein
DNA replication licensing factor MCM4
Gene
Mcm4, Cdc21, Mcmd4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity By similarity.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi509 – 5168ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent DNA helicase activity Source: Ensembl
  3. protein binding Source: MGI
  4. single-stranded DNA binding Source: MGI

GO - Biological processi

  1. DNA replication initiation Source: InterPro
  2. DNA unwinding involved in DNA replication Source: MGI
  3. cell cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_217374. Unwinding of DNA.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM4 (EC:3.6.4.12)
Alternative name(s):
CDC21 homolog
P1-CDC21
Gene namesi
Name:Mcm4
Synonyms:Cdc21, Mcmd4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:103199. Mcm4.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. MCM complex Source: UniProtKB
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 862861DNA replication licensing factor MCM4
PRO_0000194102Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei26 – 261Phosphoserine By similarity
Modified residuei31 – 311Phosphoserine By similarity
Modified residuei32 – 321Phosphoserine By similarity
Modified residuei119 – 1191Phosphoserine By similarity
Modified residuei130 – 1301Phosphoserine By similarity
Modified residuei141 – 1411Phosphoserine By similarity
Modified residuei144 – 1441Phosphoserine By similarity
Modified residuei219 – 2191N6-acetyllysine By similarity
Modified residuei449 – 4491N6-acetyllysine By similarity
Modified residuei857 – 8571N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP49717.
PaxDbiP49717.
PRIDEiP49717.

PTM databases

PhosphoSiteiP49717.

Expressioni

Gene expression databases

ArrayExpressiP49717.
BgeeiP49717.
GenevestigatoriP49717.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with MCMBP (By simililarity).

Protein-protein interaction databases

BioGridi201347. 3 interactions.
IntActiP49717. 2 interactions.
MINTiMINT-1867081.

Structurei

3D structure databases

ProteinModelPortaliP49717.
SMRiP49717. Positions 158-765.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini457 – 666210MCM
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi641 – 6444Arginine finger

Sequence similaritiesi

Belongs to the MCM family.
Contains 1 MCM domain.

Phylogenomic databases

eggNOGiCOG1241.
HOGENOMiHOG000224127.
HOVERGENiHBG102781.
InParanoidiP49717.
KOiK02212.
OMAiMHSSAIP.
OrthoDBiEOG78D7JF.
PhylomeDBiP49717.
TreeFamiTF300463.

Family and domain databases

Gene3Di2.20.28.10. 1 hit.
2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR008047. MCM_4.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR004039. Rubredoxin-type_fold.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01660. MCMPROTEIN4.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49717-1 [UniParc]FASTAAdd to Basket

« Hide

MSSPASTPSR RSSRRGRVTP TQSLRSEESR SSPNRRRRGE DSSTGELLPM    50
PTSPGADLQS PPAQNALFSS PPQMHSLAIP LDFDVSSPLT YGTPSSRVEG 100
TPRSGVRGTP VRQRPDLGSA RKGLQVDLQS DGAAAEDIVP SEQSLGQKLV 150
IWGTDVNVAT CKENFQRFLQ CFTDPLAKEE ENVGIDITQP LYMQQLGEIN 200
ITGEPFLNVN CEHIKSFSKN LYRQLISYPQ EVIPTFDMAV NEIFFDRYPD 250
SILEHQIQVR PFNALKTKSM RNLNPEDIDQ LITISGMVIR TSQLIPEMQE 300
AFFQCQVCAH TTRVEIDRGR IAEPCSCVHC HTTHSMALIH NRSFFSDKQM 350
IKLQESPEDM PAGQTPHTIV LFAHNDLVDK VQPGDRVNVT GIYRAVPIRV 400
NPRVSNVKSV YKTHIDVIHY RKTDAKRLHG LDEEAEQKLF SEKRVKLLKE 450
LSRKPDIYER LASALAPSIY EHEDIKKGIL LQLFGGTRKD FSHTGRGKFR 500
AEINILLCGD PGTSKSQLLQ YVYNLVPRGQ YTSGKGSSAV GLTAYVMKDP 550
ETRQLVLQTG ALVLSDNGIC CIDEFDKMNE STRSVLHEVM EQQTLSIAKA 600
GIICQLNART SVLAAANPIE SQWNPKKTTI ENIQLPHTLL SRFDLIFLML 650
DPQDEAYDRR LAHHLVSLYY QSEEQVEEEF LDMAVLKDYI AYAHSTIMPR 700
LSEEASQALI EAYVNMRKIG SSRGMVSAYP RQLESLIRLA EAHAKVRFSN 750
KVEAIDVEEA KRLHREALKQ SATDPRTGIV DISILTTGMS ATSRKRKEEL 800
AEALRKLILS KGKTPALKYQ QLFEDIRGQS DTAITKDMFE EALRALADDD 850
FLTVTGKTVR LL 862
Length:862
Mass (Da):96,736
Last modified:October 1, 1996 - v1
Checksum:i516ACC1A3C6FB16E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti530 – 5301Q → R in AAC36509. 1 Publication
Sequence conflicti572 – 5721I → T in AAC36509. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26089 mRNA. Translation: BAA05082.1.
U89402 mRNA. Translation: AAC36509.1.
CCDSiCCDS27977.1.
PIRiS56766.
RefSeqiNP_032591.3. NM_008565.3.
UniGeneiMm.1500.

Genome annotation databases

EnsembliENSMUST00000023353; ENSMUSP00000023353; ENSMUSG00000022673.
GeneIDi17217.
KEGGimmu:17217.
UCSCiuc007yhr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26089 mRNA. Translation: BAA05082.1 .
U89402 mRNA. Translation: AAC36509.1 .
CCDSi CCDS27977.1.
PIRi S56766.
RefSeqi NP_032591.3. NM_008565.3.
UniGenei Mm.1500.

3D structure databases

ProteinModelPortali P49717.
SMRi P49717. Positions 158-765.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201347. 3 interactions.
IntActi P49717. 2 interactions.
MINTi MINT-1867081.

PTM databases

PhosphoSitei P49717.

Proteomic databases

MaxQBi P49717.
PaxDbi P49717.
PRIDEi P49717.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023353 ; ENSMUSP00000023353 ; ENSMUSG00000022673 .
GeneIDi 17217.
KEGGi mmu:17217.
UCSCi uc007yhr.2. mouse.

Organism-specific databases

CTDi 4173.
MGIi MGI:103199. Mcm4.

Phylogenomic databases

eggNOGi COG1241.
HOGENOMi HOG000224127.
HOVERGENi HBG102781.
InParanoidi P49717.
KOi K02212.
OMAi MHSSAIP.
OrthoDBi EOG78D7JF.
PhylomeDBi P49717.
TreeFami TF300463.

Enzyme and pathway databases

Reactomei REACT_217374. Unwinding of DNA.

Miscellaneous databases

NextBioi 291610.
PROi P49717.
SOURCEi Search...

Gene expression databases

ArrayExpressi P49717.
Bgeei P49717.
Genevestigatori P49717.

Family and domain databases

Gene3Di 2.20.28.10. 1 hit.
2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR008047. MCM_4.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR004039. Rubredoxin-type_fold.
[Graphical view ]
Pfami PF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view ]
PRINTSi PR01657. MCMFAMILY.
PR01660. MCMPROTEIN4.
SMARTi SM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of cDNA encoding mouse Cdc21 and CDC46 homologs and characterization of the products: physical interaction between P1(MCM3) and CDC46 proteins."
    Kimura H., Takizawa N., Nozaki N., Sugimoto K.
    Nucleic Acids Res. 23:2097-2104(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Expressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis."
    Chu C.C., Paul W.E.
    Mol. Immunol. 35:487-502(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 503-602.
    Strain: BALB/c.
    Tissue: Spleen.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-857, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiMCM4_MOUSE
AccessioniPrimary (citable) accession number: P49717
Secondary accession number(s): O89056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi