Reviewed, UniProtKB/Swiss-Prot P49715 (CEBPA_HUMAN)

Names and origin

Protein names

Recommended name:
    CCAAT/enhancer-binding protein alpha
      Short name=C/EBP alpha

Gene names

Name:

CEBPA

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
Subunit structure	Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with UBN1. Interacts with HBV protein X. Ref.5 Ref.6
Subcellular location	Nucleus.
Sequence similarities	Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP domain.

Ontologies

Keywords
Biological process	Host-virus interaction Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
Gene Ontology (GO)
Biological process	cytokine-mediated signaling pathway Non-traceable author statement. Source: UniProtKB generation of precursor metabolites and energy Traceable author statement. Source: ProtInc interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Traceable author statement. Source: ProtInc
Molecular function	RNA polymerase II transcription factor activity, enhancer binding Ref.1 Non-traceable author statement. Source: UniProtKB transcription factor binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PARP1	P09874	1	EBI-1172054,EBI-355676

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	CCAAT/enhancer-binding protein alpha		PRO_0000076613
Regions
	Domain	317 – 345	29	Leucine-zipper
	DNA binding	286 – 306	21	Basic motif
	Compositional bias	99 – 104	6	Poly-Gly
	Compositional bias	183 – 189	7	Poly-Pro
Experimental info
	Sequence conflict	40 – 41	2	AQ → PK in AAC50235. Ref.1
	Sequence conflict	95 – 98	4	VGPT → WAH in CAA72289. Ref.2
	Sequence conflict	241	1	L → V in CAA72289. Ref.2
	Sequence conflict	248 – 250	3	GPG → ALA in CAA72289. Ref.2
	Sequence conflict	269	1	S → T in AAC50235. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49715-1 [UniParc]. Last modified February 5, 2008. Version 3. Checksum: 574C0A049E25BCAC	FASTA	358	37,561
	10 20 30 40 50 60 MESADFYEAE PRPPMSSHLQ SPPHAPSSAA FGFPRGAGPA QPPAPPAAPE PLGGICEHET 70 80 90 100 110 120 SIDISAYIDP AAFNDEFLAD LFQHSRQQEK AKAAVGPTGG GGGGDFDYPG APAGPGGAVM 130 140 150 160 170 180 PGGAHGPPPG YGCAAAGYLD GRLEPLYERV GAPALRPLVI KQEPREEDEA KQLALAGLFP 190 200 210 220 230 240 YQPPPPPPPS HPHPHPPPAH LAAPHLQFQI AHCGQTTMHL QPGHPTPPPT PVPSPHPAPA 250 260 270 280 290 300 LGAAGLPGPG SALKGLGAAH PDLRASGGSG AGKAKKSVDK NSNEYRVRRE RNNIAVRKSR 310 320 330 340 350 DKAKQRNVET QQKVLELTSD NDRLRKRVEQ LSRELDTLRG IFRQLPESSL VKAMGNCA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha)." Antonson P., Xanthopoulos K.G. Biochem. Biophys. Res. Commun. 215:106-113(1995) [PubMed: 7575576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Umbilical cord.
[2]	"Transcription factor C/EBP-alpha: novel sites of expression and cloning of the human gene." Swart G.W.M., van Groningen J.J.M., van Ruissen F., Bergers M., Schalwijk J. Biol. Chem. Hoppe-Seyler 378:373-379(1997) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	SeattleSNPs variation discovery resource Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-133. Tissue: Pancreas.
[5]	"Interaction of hepatitis B viral X protein and CCAAT/enhancer-binding protein alpha synergistically activates the hepatitis B viral enhancer II/pregenomic promoter." Choi B.H., Park G.T., Rho H.M. J. Biol. Chem. 274:2858-2865(1999) [PubMed: 9915821] [Abstract] Cited for: INTERACTION WITH HBV PROTEIN X.
[6]	"Ubinuclein, a novel nuclear protein interacting with cellular and viral transcription factors." Aho S., Buisson M., Pajunen T., Ryoo Y.W., Giot J.-F., Gruffat H., Sergeant A., Uitto J. J. Cell Biol. 148:1165-1176(2000) [PubMed: 10725330] [Abstract] Cited for: INTERACTION WITH UBN1.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

SeattleSNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	U34070 Genomic DNA. Translation: AAC50235.1. Y11525 mRNA. Translation: CAA72289.1. EU048234 Genomic DNA. Translation: ABS82765.1. BC027902 mRNA. Translation: AAH27902.1.
IPI	IPI00292025.
PIR	JC4311.
RefSeq	NP_004355.2.
UniGene	Hs.699463
3D structure databases
HSSP	HSSP built from PDB template 1NWQ based on UniProtKB P05554.
SMR	P49715. Positions 281-340.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49715. 4 interactions.
PTM databases
PhosphoSite	P49715.
Genome annotation databases
Ensembl	ENSG00000184771. Homo sapiens. [Contig view]
GeneID	1050.
KEGG	hsa:1050.
Organism-specific databases
GeneCards	GC19M038482.
HGNC	HGNC:1833. CEBPA.
MIM	116897. gene.
PharmGKB	PA26376.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P49715.
OMA	P49715. MHLQPGH.
Enzyme and pathway databases
Pathway_Interaction_DB	hnf3bpathway. FOXA2 and FOXA3 transcription factor networks. ar_tf_pathway. Regulation of Androgen receptor activity.
Gene expression databases
CleanEx	HS_CEBPA.
Family and domain databases
InterPro	IPR011700. bZIP_2. IPR016468. CCAAT/enhancer-binding. IPR004827. TF_bZIP. [Graphical view]
Pfam	PF07716. bZIP_2. 1 hit. [Graphical view]
PIRSF	PIRSF005879. CCAAT/enhancer-binding. 1 hit.
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	4397.
SOURCE	Search...

Entry information

Entry name

CEBPA_HUMAN

Accession

Primary (citable) accession number: P49715
Secondary accession number(s): A7LNP2, P78319, Q05CA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	February 5, 2008
Last modified:	June 16, 2009
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)