P49715 (CEBPA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CCAAT/enhancer-binding protein alpha
Short name=C/EBP alpha

Gene names

Name:

CEBPA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
Subunit structure	Interacts with PRDM16 By similarity. Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with UBN1. Interacts with HBV protein X. Interacts with ZNF638; this interaction increases transcriptional activation By similarity. Ref.5 Ref.6
Subcellular location	Nucleus.
Sequence similarities	Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
Biological process	Host-virus interaction Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	acute-phase response Inferred from electronic annotation. Source: Compara brown fat cell differentiation Inferred from electronic annotation. Source: Compara cell maturation Inferred from electronic annotation. Source: Compara cellular response to lithium ion Inferred from electronic annotation. Source: Compara cellular response to organic cyclic compound Inferred from electronic annotation. Source: Compara cholesterol metabolic process Inferred from electronic annotation. Source: Compara cytokine-mediated signaling pathway Non-traceable author statement PubMed 15664994. Source: UniProtKB embryonic placenta development Inferred from electronic annotation. Source: Compara generation of precursor metabolites and energy Traceable author statement PubMed 8643605. Source: ProtInc inner ear development Inferred from electronic annotation. Source: Compara liver development Inferred from electronic annotation. Source: Compara lung development Inferred from electronic annotation. Source: Compara macrophage differentiation Inferred from electronic annotation. Source: Compara mitochondrion organization Inferred from electronic annotation. Source: Compara myeloid cell differentiation Non-traceable author statement PubMed 15664994. Source: UniProtKB negative regulation of cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara organ regeneration Inferred from electronic annotation. Source: Compara positive regulation of fat cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of osteoblast differentiation Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase III promoter Inferred from direct assay PubMed 12695546. Source: UniProtKB response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara response to vitamin B2 Inferred from electronic annotation. Source: Compara urea cycle Inferred from electronic annotation. Source: Compara virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW white fat cell differentiation Inferred from electronic annotation. Source: Compara
Cellular_component	Rb-E2F complex Inferred from electronic annotation. Source: Compara nuclear matrix Inferred from electronic annotation. Source: Compara nucleus Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Non-traceable author statement Ref.1. Source: UniProtKB sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription regulatory region DNA binding Inferred from direct assay PubMed 12695546. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PARP1	P09874	2	EBI-1172054,EBI-355676

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	CCAAT/enhancer-binding protein alpha		PRO_0000076613
Regions
	Domain	282 – 345	64	bZIP
	Region	286 – 313	28	Basic motif By similarity
	Region	317 – 345	29	Leucine-zipper By similarity
	Compositional bias	99 – 104	6	Poly-Gly
	Compositional bias	183 – 189	7	Poly-Pro
Amino acid modifications
	Modified residue	161	1	N6-acetyllysine Ref.7
Experimental info
	Sequence conflict	40 – 41	2	AQ → PK in AAC50235. Ref.1
	Sequence conflict	95 – 98	4	VGPT → WAH in CAA72289. Ref.2
	Sequence conflict	241	1	L → V in CAA72289. Ref.2
	Sequence conflict	248 – 250	3	GPG → ALA in CAA72289. Ref.2
	Sequence conflict	269	1	S → T in AAC50235. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49715 [UniParc]. Last modified February 5, 2008. Version 3. Checksum: 574C0A049E25BCAC	FASTA	358	37,561
	10 20 30 40 50 60 MESADFYEAE PRPPMSSHLQ SPPHAPSSAA FGFPRGAGPA QPPAPPAAPE PLGGICEHET 70 80 90 100 110 120 SIDISAYIDP AAFNDEFLAD LFQHSRQQEK AKAAVGPTGG GGGGDFDYPG APAGPGGAVM 130 140 150 160 170 180 PGGAHGPPPG YGCAAAGYLD GRLEPLYERV GAPALRPLVI KQEPREEDEA KQLALAGLFP 190 200 210 220 230 240 YQPPPPPPPS HPHPHPPPAH LAAPHLQFQI AHCGQTTMHL QPGHPTPPPT PVPSPHPAPA 250 260 270 280 290 300 LGAAGLPGPG SALKGLGAAH PDLRASGGSG AGKAKKSVDK NSNEYRVRRE RNNIAVRKSR 310 320 330 340 350 DKAKQRNVET QQKVLELTSD NDRLRKRVEQ LSRELDTLRG IFRQLPESSL VKAMGNCA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha)." Antonson P., Xanthopoulos K.G. Biochem. Biophys. Res. Commun. 215:106-113(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Umbilical cord.
[2]	"Transcription factor C/EBP-alpha: novel sites of expression and cloning of the human gene." Swart G.W.M., van Groningen J.J.M., van Ruissen F., Bergers M., Schalwijk J. Biol. Chem. Hoppe-Seyler 378:373-379(1997) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	SeattleSNPs variation discovery resource Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-133. Tissue: Pancreas.
[5]	"Interaction of hepatitis B viral X protein and CCAAT/enhancer-binding protein alpha synergistically activates the hepatitis B viral enhancer II/pregenomic promoter." Choi B.H., Park G.T., Rho H.M. J. Biol. Chem. 274:2858-2865(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HBV PROTEIN X.
[6]	"Ubinuclein, a novel nuclear protein interacting with cellular and viral transcription factors." Aho S., Buisson M., Pajunen T., Ryoo Y.W., Giot J.-F., Gruffat H., Sergeant A., Uitto J. J. Cell Biol. 148:1165-1176(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UBN1.
[7]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-161, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

SeattleSNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34070 Genomic DNA. Translation: AAC50235.1. Y11525 mRNA. Translation: CAA72289.1. EU048234 Genomic DNA. Translation: ABS82765.1. BC027902 mRNA. Translation: AAH27902.1.
IPI	IPI00292025.
PIR	JC4311.
RefSeq	NP_004355.2. NM_004364.3.
UniGene	Hs.740432.
3D structure databases
ProteinModelPortal	P49715.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49715. 6 interactions.
MINT	MINT-264048.
PTM databases
PhosphoSite	P49715.
Polymorphism databases
DMDM	166898082.
Proteomic databases
PRIDE	P49715.
Protocols and materials databases
DNASU	1050.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000498907; ENSP00000427514; ENSG00000245848.
GeneID	1050.
KEGG	hsa:1050.
UCSC	uc002nun.3. human.
Organism-specific databases
CTD	1050.
GeneCards	GC19M033790.
H-InvDB	HIX0040095.
HGNC	HGNC:1833. CEBPA.
MIM	116897. gene.
neXtProt	NX_P49715.
Orphanet	102724. Acute myeloid leukemia with t(8;21)(q22;q22) translocation.
PharmGKB	PA26376.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HOG000013112.
HOVERGEN	HBG050879.
InParanoid	P49715.
KO	K09055.
OMA	DICENEN.
OrthoDB	EOG4M65JR.
Enzyme and pathway databases
Pathway_Interaction_DB	hnf3bpathway. FOXA2 and FOXA3 transcription factor networks. ar_tf_pathway. Regulation of Androgen receptor activity.
Reactome	REACT_111045. Developmental Biology.
Gene expression databases
ArrayExpress	P49715.
Bgee	P49715.
CleanEx	HS_CEBPA.
Genevestigator	P49715.
Family and domain databases
InterPro	IPR004827. bZIP. IPR016468. CCAAT/enhancer-binding. [Graphical view]
Pfam	PF07716. bZIP_2. 1 hit. [Graphical view]
PIRSF	PIRSF005879. CCAAT/enhancer-binding. 1 hit.
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	1050.
NextBio	4397.
SOURCE	Search...

Entry information

Entry name

CEBPA_HUMAN

Accession

Primary (citable) accession number: P49715
Secondary accession number(s): A7LNP2, P78319, Q05CA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	February 5, 2008
Last modified:	May 1, 2013
This is version 125 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.