Reviewed, UniProtKB/Swiss-Prot P49714 (ENAN_BPK1E)

Names and origin

Protein names

Recommended name:
    Endo-N-acetylneuraminidase
      Short name=Endo-N
    EC=3.2.1.129
Alternative name(s):
    Endosialidase

Gene names

Name:	GP90
Synonyms:	END

Organism

Enterobacteria phage K1E (Bacteriophage K1E)

Taxonomic identifier

344022 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Autographivirinae

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	811 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for initial absorption of the phage to the host bacterium. Degradation of the alpha-2,8-linked polysialic acid K1 capsule.
Catalytic activity	Endohydrolysis of (2->8)-alpha-sialosyl linkages in oligo- or poly(sialic) acids.
Subunit structure	Homotrimer.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the glycosyl hydrolase 58 family. Contains 3 BNR repeats.

Ontologies

Keywords
Domain	Repeat
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	endo-alpha-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 811	811	Endo-N-acetylneuraminidase		PRO_0000057708
Regions
	Repeat	150 – 161	12	BNR 1
	Repeat	286 – 293	8	BNR 2
	Repeat	398 – 409	12	BNR 3
	Compositional bias	642 – 645	4	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	P49714-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 8F96F465ED533B6E	FASTA	811	90,524
	10 20 30 40 50 60 MIQRLGSSLV KFKSKIAGAI WRNLDDKLTE VVSLKDFGAK GDGKTNDQDA VNAAMASGKR 70 80 90 100 110 120 IDGAGATYKV SSLPDMERFY NTRFVWERLA GQPLYYVSKG FINGELYKIT DNPYYNAWPQ 130 140 150 160 170 180 DKAFVYENVI YAPYMGSDRH GVSRLHVSWV KSGDDGQTWS TPEWLTDMHP DYPTVNYHCM 190 200 210 220 230 240 SMGVCRNRLF AMIETRTLAK NELTNCALWD RPMSRSLHLT GGITKAANQR YATIHVPDHG 250 260 270 280 290 300 LFVGDFVNFS NSAVTGVSGD MKVATVIDKD NFTVLTPNQQ TSDLNNAGKN WHMGTSFHKS 310 320 330 340 350 360 PWRKTDLGLI PRVTEVHSFA TIDNNGFVMG YHQGDVAPRE VGLFYFPDAF NSPSNYVRRQ 370 380 390 400 410 420 IPSEYEPDAA EPCIKYYDGV LYLITRGTRG DRLGSSLHRS RDIGQTWESL RFPHNVHHTT 430 440 450 460 470 480 LPFAKVGDDL IMFGSERAEN EWEAGAPDDR YKASYPRTFY ARLNVNNWNA DDIEWVNITD 490 500 510 520 530 540 QIYQGDIVNS SVGVGSVVVK DSFIYYIFGG ENHFNPMTYG DNKDKDPFKG HGHPTDIYCY 550 560 570 580 590 600 KMQIANDNRV SRKFTYGATP GQAIPTFMGT DGIRNIPAPL YFSDNIVTED TKVGHLTLKA 610 620 630 640 650 660 STSANIRSEM QMEGEYGFIG KSVPKDKPTG QRLIICGGEG TSSSSGAQIT LHGSNSSNAK 670 680 690 700 710 720 RITYNGNEHL FQGAPIMPAV DNQFAAGGPS NRFTTIYLGS DPVTTSDADH KYGISSINTK 730 740 750 760 770 780 VLKAWSRVGF KQYGLNSEAE RNLDSIHFGV LAQDIVAAFE AEGLDAIKYG IVSFEEGRYG 790 800 810 VRYSEVLILE AAYTRHRLDK LEEMYATNKI S

References

[1]	"Complete nucleotide sequence of the gene encoding bacteriophage E endosialidase: implications for K1E endosialidase structure and function." Long G.S., Bryant J.M., Taylor P.W., Luzio J.P. Biochem. J. 309:543-550(1995) [PubMed: 7626018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE OF 1-6; 330-349 AND 613-632. Strain: ATCC 40221.
[2]	Gerardy-Schahn R., Hansen A., Brennecke T., Muehlenhoff M., Eckhardt M., Ziesing S., Lottspeich F., Frosch M. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Muehlenhoff M. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 53-56; 523 AND 539.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X78310 Genomic DNA. Translation: CAA55120.1. Z36986 Genomic DNA. Translation: CAA85449.2.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH58. Glycoside Hydrolase Family 58.
Family and domain databases
InterPro	IPR001724. Glyco_hydro_58. [Graphical view]
PRINTS	PR00849. GLHYDRLASE58.
ProtoNet	Search...

Entry information

Entry name

ENAN_BPK1E

Accession

Primary (citable) accession number: P49714

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 14, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)