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Protein

Hematopoietic lineage cell-specific protein

Gene

Hcls1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression (By similarity).By similarity

GO - Molecular functioni

  • protein complex binding Source: MGI
  • protein kinase binding Source: MGI
  • RNA polymerase II transcription factor binding Source: MGI
  • SH3 domain binding Source: MGI

GO - Biological processi

  • actin filament polymerization Source: InterPro
  • cellular response to cytokine stimulus Source: MGI
  • erythrocyte differentiation Source: UniProtKB
  • negative regulation of leukocyte apoptotic process Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of granulocyte differentiation Source: BHF-UCL
  • positive regulation of macrophage differentiation Source: BHF-UCL
  • positive regulation of peptidyl-serine phosphorylation Source: MGI
  • positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
  • positive regulation of protein kinase B signaling Source: MGI
  • positive regulation of transcription factor import into nucleus Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of tyrosine phosphorylation of STAT protein Source: UniProtKB
  • regulation of actin filament polymerization Source: MGI
  • response to hormone Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Hematopoietic lineage cell-specific protein
Alternative name(s):
Hematopoietic cell-specific LYN substrate 1
LckBP1
Gene namesi
Name:Hcls1
Synonyms:Hs1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:104568. Hcls1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • mitochondrion Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi388Y → F: Strongly reduces phosphorylation by FES. Abolishes phosphorylation by FES; when associated with F-405. 1 Publication1
Mutagenesisi405Y → F: Minor effect on phosphorylation by FES. Abolishes phosphorylation by FES; when associated with F-388. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000839221 – 486Hematopoietic lineage cell-specific proteinAdd BLAST486

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41N6-acetyllysineBy similarity1
Modified residuei123N6-acetyllysineBy similarity1
Modified residuei140PhosphotyrosineCombined sources1
Modified residuei192N6-acetyllysineBy similarity1
Modified residuei198PhosphotyrosineBy similarity1
Modified residuei222Phosphotyrosine; by FGRBy similarity1
Modified residuei241N6-acetyllysineBy similarity1
Modified residuei275PhosphoserineBy similarity1
Modified residuei330PhosphothreonineCombined sources1
Modified residuei333PhosphoserineCombined sources1
Modified residuei388Phosphotyrosine; by SYK and FES1 Publication1
Modified residuei405Phosphotyrosine; by SYK and FES1 Publication1

Post-translational modificationi

Phosphorylated by LYN, FYN and FGR after cross-linking of surface IgM on B-cells. Phosphorylation by LYN, FYN and FGR requires prior phosphorylation by SYK (By similarity). Binds to LCK in vivo, and is tyrosine phosphorylated upon TCR stimulation. Phosphorylated by FES.By similarity1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP49710.
MaxQBiP49710.
PaxDbiP49710.
PeptideAtlasiP49710.
PRIDEiP49710.

PTM databases

iPTMnetiP49710.
PhosphoSitePlusiP49710.
SwissPalmiP49710.

Miscellaneous databases

PMAP-CutDBP49710.

Expressioni

Tissue specificityi

Expressed only in tissues and cells of hematopoietic origin.

Gene expression databases

BgeeiENSMUSG00000022831.
CleanExiMM_HCLS1.
ExpressionAtlasiP49710. baseline and differential.
GenevisibleiP49710. MM.

Interactioni

Subunit structurei

Interacts (via SH2 domain) with FGR (By similarity). Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS. Forms a multiprotein complex with LYN and ANKRD54.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LynP2591110EBI-924601,EBI-643537

GO - Molecular functioni

  • protein complex binding Source: MGI
  • protein kinase binding Source: MGI
  • RNA polymerase II transcription factor binding Source: MGI
  • SH3 domain binding Source: MGI

Protein-protein interaction databases

BioGridi200250. 2 interactors.
IntActiP49710. 4 interactors.
MINTiMINT-84934.
STRINGi10090.ENSMUSP00000023531.

Structurei

3D structure databases

ProteinModelPortaliP49710.
SMRiP49710.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati79 – 115Cortactin 1Add BLAST37
Repeati116 – 152Cortactin 2Add BLAST37
Repeati153 – 189Cortactin 3Add BLAST37
Repeati190 – 212Cortactin 4; truncatedAdd BLAST23
Domaini429 – 486SH3PROSITE-ProRule annotationAdd BLAST58

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 66Involved in HAX-1 bindingBy similarityAdd BLAST40

Sequence similaritiesi

Contains 4 cortactin repeats.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiENOG410IFV2. Eukaryota.
ENOG410XTAK. LUCA.
GeneTreeiENSGT00530000062953.
HOGENOMiHOG000006523.
HOVERGENiHBG005994.
InParanoidiP49710.
KOiK06106.
OMAiEMDRHEQ.
OrthoDBiEOG091G0CPX.
TreeFamiTF318935.

Family and domain databases

InterProiIPR028534. HS1.
IPR003134. Hs1_Cortactin.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10829:SF5. PTHR10829:SF5. 1 hit.
PfamiPF02218. HS1_rep. 4 hits.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51090. CORTACTIN. 4 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49710-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWKSVVGHDV SVSVETQGDD WDTDPDFVND ISEKEQRWGA KTIEGSGRTE
60 70 80 90 100
HINIHQLRNK VSEEHDILKK KELESGPKAS HGYGGRFGVE RDRMDKSAVG
110 120 130 140 150
HEYVADVEKH SSQTDAARGF GGKYGVERDR ADKSAVGFDY KGEVEKHASQ
160 170 180 190 200
KDYSHGFGGR YGVEKDKRDK AALGYDYKGE TEKHESQRDY AKGFGGQYGI
210 220 230 240 250
QKDRVDKSAV GFNEMEAPTT AYKKTTPIEA ASSGARGLKA KFESLAEEKR
260 270 280 290 300
KREEEEKAQQ MARQQQERKA VVKMSREVQQ PSMPVEEPAA PAQLPKKISS
310 320 330 340 350
EVWPPAESHL PPESQPVRSR REYPVPSLPT RQSPLQNHLE DNEEPPALPP
360 370 380 390 400
RTPEGLQVVE EPVYEAAPEL EPEPEPDYEP EPETEPDYED VGELDRQDED
410 420 430 440 450
AEGDYEDVLE PEDTPSLSYQ AGPSAGAGGA GISAIALYDY QGEGSDELSF
460 470 480
DPDDIITDIE MVDEGWWRGQ CRGHFGLFPA NYVKLL
Length:486
Mass (Da):54,240
Last modified:July 27, 2011 - v2
Checksum:i0EE14FEFA0A31412
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86R → Q in BAA07701 (PubMed:7535527).Curated1
Sequence conflicti86R → Q in CAA59265 (PubMed:7628441).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42120 mRNA. Translation: BAA07701.1.
X84797 mRNA. Translation: CAA59265.1.
BC007469 mRNA. Translation: AAH07469.1.
CCDSiCCDS37336.1.
PIRiI49760.
RefSeqiNP_032251.2. NM_008225.2.
UniGeneiMm.4091.

Genome annotation databases

EnsembliENSMUST00000023531; ENSMUSP00000023531; ENSMUSG00000022831.
GeneIDi15163.
KEGGimmu:15163.
UCSCiuc007zdi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42120 mRNA. Translation: BAA07701.1.
X84797 mRNA. Translation: CAA59265.1.
BC007469 mRNA. Translation: AAH07469.1.
CCDSiCCDS37336.1.
PIRiI49760.
RefSeqiNP_032251.2. NM_008225.2.
UniGeneiMm.4091.

3D structure databases

ProteinModelPortaliP49710.
SMRiP49710.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200250. 2 interactors.
IntActiP49710. 4 interactors.
MINTiMINT-84934.
STRINGi10090.ENSMUSP00000023531.

PTM databases

iPTMnetiP49710.
PhosphoSitePlusiP49710.
SwissPalmiP49710.

Proteomic databases

EPDiP49710.
MaxQBiP49710.
PaxDbiP49710.
PeptideAtlasiP49710.
PRIDEiP49710.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023531; ENSMUSP00000023531; ENSMUSG00000022831.
GeneIDi15163.
KEGGimmu:15163.
UCSCiuc007zdi.2. mouse.

Organism-specific databases

CTDi3059.
MGIiMGI:104568. Hcls1.

Phylogenomic databases

eggNOGiENOG410IFV2. Eukaryota.
ENOG410XTAK. LUCA.
GeneTreeiENSGT00530000062953.
HOGENOMiHOG000006523.
HOVERGENiHBG005994.
InParanoidiP49710.
KOiK06106.
OMAiEMDRHEQ.
OrthoDBiEOG091G0CPX.
TreeFamiTF318935.

Miscellaneous databases

PMAP-CutDBP49710.
PROiP49710.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022831.
CleanExiMM_HCLS1.
ExpressionAtlasiP49710. baseline and differential.
GenevisibleiP49710. MM.

Family and domain databases

InterProiIPR028534. HS1.
IPR003134. Hs1_Cortactin.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10829:SF5. PTHR10829:SF5. 1 hit.
PfamiPF02218. HS1_rep. 4 hits.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51090. CORTACTIN. 4 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHCLS1_MOUSE
AccessioniPrimary (citable) accession number: P49710
Secondary accession number(s): Q922I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.