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Protein

Pre-mRNA-processing factor 31

Gene

PRP31

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Promotes the association of the U4/U6.U5 tri-snRNP particle with pre-spliceosomes to form the mature spliceosomal complex.1 Publication

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
  • spliceosomal snRNP assembly Source: SGD
  • spliceosomal tri-snRNP complex assembly Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30801-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing factor 31
Gene namesi
Name:PRP31
Ordered Locus Names:YGR091W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR091W.
SGDiS000003323. PRP31.

Subcellular locationi

GO - Cellular componenti

  • spliceosomal complex Source: UniProtKB-KW
  • U4/U6 x U5 tri-snRNP complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Pre-mRNA-processing factor 31PRO_0000058589Add
BLAST

Proteomic databases

MaxQBiP49704.

Interactioni

Subunit structurei

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.5 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei257 – 2571Interaction with U4 snRNABy similarity

Protein-protein interaction databases

BioGridi33334. 84 interactions.
DIPiDIP-5355N.
IntActiP49704. 33 interactions.
MINTiMINT-493013.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JCMelectron microscopy3.80I1-494[»]
5GANelectron microscopy3.60F1-494[»]
5GAPelectron microscopy3.60F1-494[»]
ProteinModelPortaliP49704.
SMRiP49704. Positions 99-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini225 – 346122NopPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili88 – 12235By similarityAdd
BLAST
Coiled coili191 – 22535By similarityAdd
BLAST

Domaini

Interacts with the snRNP via the Nop domain.By similarity
The coiled coil domain is formed by two non-contiguous helices.By similarity

Sequence similaritiesi

Belongs to the PRP31 family.Curated
Contains 1 Nop domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000075069.
HOGENOMiHOG000000957.
InParanoidiP49704.
KOiK12844.
OMAiHRISEAN.
OrthoDBiEOG7JDR8D.

Family and domain databases

InterProiIPR002687. Nop_dom.
IPR012976. NOSIC.
IPR027105. Prp31.
IPR019175. Prp31_C.
[Graphical view]
PANTHERiPTHR13904. PTHR13904. 1 hit.
PfamiPF01798. Nop. 1 hit.
PF09785. Prp31_C. 1 hit.
[Graphical view]
SMARTiSM00931. NOSIC. 1 hit.
[Graphical view]
SUPFAMiSSF89124. SSF89124. 1 hit.
PROSITEiPS51358. NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49704-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSEEDYFDE LEYDLADEVN EEKEDIQTKK LTTVNCQTEK FNPFEILPES
60 70 80 90 100
IELFRTLALI SPDRLSLSET AQILPKIVDL KRILQQQEID FIKLLPFFNE
110 120 130 140 150
IIPLIKSNIK LMHNFLISLY SRRFPELSSL IPSPLQYSKV ISILENENYS
160 170 180 190 200
KNESDELFFH LENKAKLTRE QILVLTMSMK TSFKNKEPLD IKTRTQILEA
210 220 230 240 250
NSILENLWKL QEDIGQYIAS KISIIAPNVC FLVGPEIAAQ LIAHAGGVLE
260 270 280 290 300
FSRIPSCNIA SIGKNKHLSH ELHTLESGVR QEGYLFASDM IQKFPVSVHK
310 320 330 340 350
QMLRMLCAKV SLAARVDAGQ KNGDRNTVLA HKWKAELSKK ARKLSEAPSI
360 370 380 390 400
SETKALPIPE DQPKKKRAGR KFRKYKEKFR LSHVRQLQNR MEFGKQEQTV
410 420 430 440 450
LDSYGEEVGL GMSNTSLQQA VGATSGSRRS AGNQAKLTKV MKHRISEANQ
460 470 480 490
QADEFLISLG HNTEQPNLSP EMVQMHKKQH TNPEEETNWF SGHG
Length:494
Mass (Da):56,303
Last modified:February 21, 2006 - v2
Checksum:iE39AC6247859FB3B
GO

Sequence cautioni

The sequence AAA74984.1 differs from that shown. Reason: Frameshift at positions 361 and 394. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311L → F in AAU09731 (PubMed:9169869).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31970 Genomic DNA. Translation: AAA74984.1. Frameshift.
Z72876 Genomic DNA. Translation: CAA97094.1.
AY723814 Genomic DNA. Translation: AAU09731.1.
BK006941 Genomic DNA. Translation: DAA08184.1.
PIRiS64386.
RefSeqiNP_011605.1. NM_001181220.1.

Genome annotation databases

EnsemblFungiiYGR091W; YGR091W; YGR091W.
GeneIDi852983.
KEGGisce:YGR091W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31970 Genomic DNA. Translation: AAA74984.1. Frameshift.
Z72876 Genomic DNA. Translation: CAA97094.1.
AY723814 Genomic DNA. Translation: AAU09731.1.
BK006941 Genomic DNA. Translation: DAA08184.1.
PIRiS64386.
RefSeqiNP_011605.1. NM_001181220.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JCMelectron microscopy3.80I1-494[»]
5GANelectron microscopy3.60F1-494[»]
5GAPelectron microscopy3.60F1-494[»]
ProteinModelPortaliP49704.
SMRiP49704. Positions 99-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33334. 84 interactions.
DIPiDIP-5355N.
IntActiP49704. 33 interactions.
MINTiMINT-493013.

Proteomic databases

MaxQBiP49704.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR091W; YGR091W; YGR091W.
GeneIDi852983.
KEGGisce:YGR091W.

Organism-specific databases

EuPathDBiFungiDB:YGR091W.
SGDiS000003323. PRP31.

Phylogenomic databases

GeneTreeiENSGT00550000075069.
HOGENOMiHOG000000957.
InParanoidiP49704.
KOiK12844.
OMAiHRISEAN.
OrthoDBiEOG7JDR8D.

Enzyme and pathway databases

BioCyciYEAST:G3O-30801-MONOMER.

Miscellaneous databases

NextBioi972794.
PROiP49704.

Family and domain databases

InterProiIPR002687. Nop_dom.
IPR012976. NOSIC.
IPR027105. Prp31.
IPR019175. Prp31_C.
[Graphical view]
PANTHERiPTHR13904. PTHR13904. 1 hit.
PfamiPF01798. Nop. 1 hit.
PF09785. Prp31_C. 1 hit.
[Graphical view]
SMARTiSM00931. NOSIC. 1 hit.
[Graphical view]
SUPFAMiSSF89124. SSF89124. 1 hit.
PROSITEiPS51358. NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae."
    Weidenhammer E.M., Singh M., Ruiz-Noriega M., Woolford J.L. Jr.
    Nucleic Acids Res. 24:1164-1170(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Prp31p promotes the association of the U4/U6.U5 tri-snRNP with prespliceosomes to form spliceosomes in Saccharomyces cerevisiae."
    Weidenhammer E.M., Ruiz-Noriega M., Woolford J.L. Jr.
    Mol. Cell. Biol. 17:3580-3588(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN U4/U6.U5 TRI-SNRNP COMPLEX.
  6. "Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
    Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
    EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins."
    Stevens S.W., Abelson J.
    Proc. Natl. Acad. Sci. U.S.A. 96:7226-7231(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN U4/U6.U5 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  8. "Composition and functional characterization of the yeast spliceosomal penta-snRNP."
    Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
    Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy."
    Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B., Stark H., Fabrizio P., Luhrmann R.
    Nat. Struct. Mol. Biol. 15:1206-1212(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, ELECTRON MICROSCOPY.

Entry informationi

Entry nameiPRP31_YEAST
AccessioniPrimary (citable) accession number: P49704
Secondary accession number(s): D6VUM3, Q66R99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: February 21, 2006
Last modified: May 11, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1260 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.