P49683 (PRLHR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prolactin-releasing peptide receptor
Short name=PrRP receptor
Short name=PrRPR

Alternative name(s):
G-protein coupled receptor 10
hGR3

Gene names

Name:	PRLHR
Synonyms:	GPR10, GR3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for prolactin-releasing peptide (PrRP). Implicated in lactation, regulation of food intake and pain-signal processing.
Subunit structure	Interacts through its C-terminal region with the PDZ domain-containing proteins GRIP1, GRIP2 and PICK1. Interacts with PDZ domains 4 and 5 of GRIP1 and with the PDZ domain of PICK1. Ref.7
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Only detected in the pituitary gland and in all cell types of pituitary adenomas. Ref.3 Ref.6
Induction	Repressed by bromocriptine, a dopamine agonist. Ref.3
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Sequence caution	The sequence AAC50504.1 differs from that shown. Reason: Frameshift at positions 168 and 175.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	feeding behavior Inferred from electronic annotation. Source: Compara female pregnancy Traceable author statement Ref.2. Source: ProtInc hormone metabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	neuropeptide Y receptor activity Inferred from electronic annotation. Source: InterPro neuropeptide receptor activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Prolactin-releasing peptide receptor		PRO_0000069524
Regions
	Topological domain	1 – 62	62	Extracellular Potential
	Transmembrane	63 – 83	21	Helical; Name=1; Potential
	Topological domain	84 – 101	18	Cytoplasmic Potential
	Transmembrane	102 – 122	21	Helical; Name=2; Potential
	Topological domain	123 – 126	4	Extracellular Potential
	Transmembrane	127 – 147	21	Helical; Name=3; Potential
	Topological domain	148 – 175	28	Cytoplasmic Potential
	Transmembrane	176 – 196	21	Helical; Name=4; Potential
	Topological domain	197 – 225	29	Extracellular Potential
	Transmembrane	226 – 246	21	Helical; Name=5; Potential
	Topological domain	247 – 276	30	Cytoplasmic Potential
	Transmembrane	277 – 297	21	Helical; Name=6; Potential
	Topological domain	298 – 317	20	Extracellular Potential
	Transmembrane	318 – 338	21	Helical; Name=7; Potential
	Topological domain	339 – 369	31	Cytoplasmic Potential
	Region	365 – 370	6	Required for interaction with GRIP1, GRIP2 and PICK1
Amino acid modifications
	Glycosylation	27	1	N-linked (GlcNAc...) Potential
	Glycosylation	36	1	N-linked (GlcNAc...) Potential
	Disulfide bond	134 ↔ 211		By similarity
Natural variations
	Natural variant	283	1	I → V. Ref.1 Ref.2 Ref.3 Ref.5 Corresponds to variant rs1613448 [ dbSNP | Ensembl ].		VAR_023948
	Natural variant	302	1	D → G. Corresponds to variant rs8192523 [ dbSNP | Ensembl ].		VAR_029746
Experimental info
	Mutagenesis	365 – 370	6	Missing: Abolishes binding to GRIP1 and PICK1. Ref.7
	Mutagenesis	365	1	T → A: No effect on binding to GRIP1. Ref.7
	Mutagenesis	366	1	V → A: No effect on binding to GRIP1. Ref.7
	Mutagenesis	367	1	S → A: Abolishes binding to GRIP1. Ref.7
	Mutagenesis	368	1	V → A: Abolishes binding to GRIP1. Ref.7
	Mutagenesis	369	1	V → A: No effect on binding to GRIP1. Ref.7
	Mutagenesis	370	1	I → A: Abolishes binding to GRIP1. Ref.7
	Sequence conflict	89	1	R → P in AAC50504. Ref.1
	Sequence conflict	194	1	A → P in AAC50504. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49683 [UniParc]. Last modified November 2, 2010. Version 3. Checksum: 47F71490A080C81F	FASTA	370	41,121
	10 20 30 40 50 60 MASSTTRGPR VSDLFSGLPP AVTTPANQSA EASAGNGSVA GADAPAVTPF QSLQLVHQLK 70 80 90 100 110 120 GLIVLLYSVV VVVGLVGNCL LVLVIARVRR LHNVTNFLIG NLALSDVLMC TACVPLTLAY 130 140 150 160 170 180 AFEPRGWVFG GGLCHLVFFL QPVTVYVSVF TLTTIAVDRY VVLVHPLRRR ISLRLSAYAV 190 200 210 220 230 240 LAIWALSAVL ALPAAVHTYH VELKPHDVRL CEEFWGSQER QRQLYAWGLL LVTYLLPLLV 250 260 270 280 290 300 ILLSYVRVSV KLRNRVVPGC VTQSQADWDR ARRRRTFCLL VVIVVVFAVC WLPLHVFNLL 310 320 330 340 350 360 RDLDPHAIDP YAFGLVQLLC HWLAMSSACY NPFIYAWLHD SFREELRKLL VAWPRKIAPH 370 GQNMTVSVVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and chromosomal mapping of three novel genes, GPR9, GPR10, and GPR14, encoding receptors related to interleukin 8, neuropeptide Y, and somatostatin receptors." Marchese A., Heiber M., Nguyen T., Heng H.H.Q., Saldivia V.R., Cheng R., Murphy P.M., Tsui L.-C., Shi X., Gregor P., George S.R., O'Dowd B.F., Docherty J.M. Genomics 29:335-344(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-283.
[2]	"A prolactin-releasing peptide in the brain." Hinuma S., Habata Y., Fujii R., Kawamata Y., Hosoya M., Fukusumi S., Kitada C., Masuo Y., Asano T., Matsumoto H., Sekiguchi M., Kurokawa T., Nishimura O., Onda H., Fujino M. Nature 393:272-276(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-283.
[3]	"Transcriptional regulation of the human PRL-releasing peptide (PrRP) receptor gene by a dopamine 2 receptor agonist: cloning and characterization of the human PrRP receptor gene and its promoter region." Ozawa A., Yamada M., Satoh T., Monden T., Hashimoto K., Kohga H., Hashiba Y., Sasaki T., Mori M. Mol. Endocrinol. 16:785-798(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION, VARIANT VAL-283.
[4]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-283.
[6]	"Tissue distribution of prolactin-releasing peptide (PrRP) and its receptor." Fujii R., Fukusumi S., Hosoya M., Kawamata Y., Habata Y., Hinuma S., Sekiguchi M., Kitada C., Kurokawa T., Nishimura O., Onda H., Sumino Y., Fujino M. Regul. Pept. 83:1-10(1999) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"The carboxyl terminus of the prolactin-releasing peptide receptor interacts with PDZ domain proteins involved in alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor clustering." Lin S.H.S., Arai A.C., Wang Z., Nothacker H.-P., Civelli O. Mol. Pharmacol. 60:916-923(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GRIP1; GRIP2 AND PICK1, MUTAGENESIS OF THR-365; VAL-366; SER-367; VAL-368; VAL-369 AND ILE-370.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U32672 Genomic DNA. Translation: AAC50504.1. Frameshift. AB015745 mRNA. Translation: BAA31159.1. AB048946 Genomic DNA. Translation: BAB83030.1. AL356865 Genomic DNA. Translation: CAH73066.1. BC095539 mRNA. Translation: AAH95539.1. BC101490 mRNA. Translation: AAI01491.1. BC101492 mRNA. Translation: AAI01493.1.
IPI	IPI00027755.
RefSeq	NP_004239.1. NM_004248.2.
UniGene	Hs.248119.
3D structure databases
ProteinModelPortal	P49683.
SMR	P49683. Positions 67-350.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1785715.
STRING	9606.ENSP00000239032.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P49683.
Polymorphism databases
DMDM	311033415.
Proteomic databases
PaxDb	P49683.
PRIDE	P49683.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000239032; ENSP00000239032; ENSG00000119973. ENST00000369169; ENSP00000358167; ENSG00000119973.
GeneID	2834.
KEGG	hsa:2834.
UCSC	uc021pzm.1. human.
Organism-specific databases
CTD	2834.
GeneCards	GC10M120344.
H-InvDB	HIX0170463.
HGNC	HGNC:4464. PRLHR.
HPA	HPA039361.
MIM	600895. gene.
neXtProt	NX_P49683.
PharmGKB	PA28847.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG267229.
HOGENOM	HOG000050229.
HOVERGEN	HBG101109.
InParanoid	P49683.
KO	K04314.
OMA	FIYAWLH.
OrthoDB	EOG4WDDC5.
PhylomeDB	P49683.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	P49683.
CleanEx	HS_PRLHR.
Genevestigator	P49683.
GermOnline	ENSG00000119973. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001402. Prolrel_pep_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01018. PRPRECEPTOR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P49683.
ChEMBL	CHEMBL1681611.
GenomeRNAi	2834.
NextBio	11185.
SOURCE	Search...

Entry information

Entry name

PRLHR_HUMAN

Accession

Primary (citable) accession number: P49683
Secondary accession number(s): O75194, Q502U8, Q5VXR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 2, 2010
Last modified:	May 1, 2013
This is version 118 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.