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Protein

Prolactin-releasing peptide receptor

Gene

PRLHR

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for prolactin-releasing peptide (PrRP). Implicated in lactation, regulation of food intake and pain-signal processing.

GO - Molecular functioni

  • G-protein coupled receptor activity Source: ProtInc
  • neuropeptide receptor activity Source: ProtInc
  • neuropeptide Y receptor activity Source: InterPro

GO - Biological processi

  • cell surface receptor signaling pathway Source: GO_Central
  • feeding behavior Source: GO_Central
  • female pregnancy Source: ProtInc
  • G-protein coupled receptor signaling pathway Source: ProtInc
  • hormone metabolic process Source: Ensembl
  • neuropeptide signaling pathway Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-HSA-375276. Peptide ligand-binding receptors.
SignaLinkiP49683.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolactin-releasing peptide receptor
Short name:
PrRP receptor
Short name:
PrRPR
Alternative name(s):
G-protein coupled receptor 10
hGR3
Gene namesi
Name:PRLHR
Synonyms:GPR10, GR3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:4464. PRLHR.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6262ExtracellularSequence analysisAdd
BLAST
Transmembranei63 – 8321Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini84 – 10118CytoplasmicSequence analysisAdd
BLAST
Transmembranei102 – 12221Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini123 – 1264ExtracellularSequence analysis
Transmembranei127 – 14721Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini148 – 17528CytoplasmicSequence analysisAdd
BLAST
Transmembranei176 – 19621Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini197 – 22529ExtracellularSequence analysisAdd
BLAST
Transmembranei226 – 24621Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini247 – 27630CytoplasmicSequence analysisAdd
BLAST
Transmembranei277 – 29721Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini298 – 31720ExtracellularSequence analysisAdd
BLAST
Transmembranei318 – 33821Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini339 – 36931CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: ProtInc
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi365 – 3706Missing : Abolishes binding to GRIP1 and PICK1.
Mutagenesisi365 – 3651T → A: No effect on binding to GRIP1. 1 Publication
Mutagenesisi366 – 3661V → A: No effect on binding to GRIP1. 1 Publication
Mutagenesisi367 – 3671S → A: Abolishes binding to GRIP1. 1 Publication
Mutagenesisi368 – 3681V → A: Abolishes binding to GRIP1. 1 Publication
Mutagenesisi369 – 3691V → A: No effect on binding to GRIP1. 1 Publication
Mutagenesisi370 – 3701I → A: Abolishes binding to GRIP1. 1 Publication

Organism-specific databases

PharmGKBiPA28847.

Chemistry

ChEMBLiCHEMBL1681611.
GuidetoPHARMACOLOGYi337.

Polymorphism and mutation databases

BioMutaiPRLHR.
DMDMi311033415.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Prolactin-releasing peptide receptorPRO_0000069524Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi27 – 271N-linked (GlcNAc...)Sequence analysis
Glycosylationi36 – 361N-linked (GlcNAc...)Sequence analysis
Disulfide bondi134 ↔ 211PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP49683.
PRIDEiP49683.

PTM databases

PhosphoSiteiP49683.

Expressioni

Tissue specificityi

Only detected in the pituitary gland and in all cell types of pituitary adenomas.2 Publications

Inductioni

Repressed by bromocriptine, a dopamine agonist.1 Publication

Gene expression databases

BgeeiP49683.
CleanExiHS_PRLHR.
GenevisibleiP49683. HS.

Organism-specific databases

HPAiHPA039361.

Interactioni

Subunit structurei

Interacts through its C-terminal region with the PDZ domain-containing proteins GRIP1, GRIP2 and PICK1. Interacts with PDZ domains 4 and 5 of GRIP1 and with the PDZ domain of PICK1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GRIP1Q9Y3R02EBI-8009236,EBI-5349621

Protein-protein interaction databases

BioGridi109095. 3 interactions.
IntActiP49683. 3 interactions.
MINTiMINT-1785715.
STRINGi9606.ENSP00000239032.

Structurei

3D structure databases

ProteinModelPortaliP49683.
SMRiP49683. Positions 67-347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni365 – 3706Required for interaction with GRIP1, GRIP2 and PICK1

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118781.
HOGENOMiHOG000050229.
HOVERGENiHBG101109.
InParanoidiP49683.
KOiK04314.
OMAiFIYAWLH.
OrthoDBiEOG75B85N.
PhylomeDBiP49683.
TreeFamiTF315303.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001402. Prolrel_pep_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01018. PRPRECEPTOR.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49683-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSTTRGPR VSDLFSGLPP AVTTPANQSA EASAGNGSVA GADAPAVTPF
60 70 80 90 100
QSLQLVHQLK GLIVLLYSVV VVVGLVGNCL LVLVIARVRR LHNVTNFLIG
110 120 130 140 150
NLALSDVLMC TACVPLTLAY AFEPRGWVFG GGLCHLVFFL QPVTVYVSVF
160 170 180 190 200
TLTTIAVDRY VVLVHPLRRR ISLRLSAYAV LAIWALSAVL ALPAAVHTYH
210 220 230 240 250
VELKPHDVRL CEEFWGSQER QRQLYAWGLL LVTYLLPLLV ILLSYVRVSV
260 270 280 290 300
KLRNRVVPGC VTQSQADWDR ARRRRTFCLL VVIVVVFAVC WLPLHVFNLL
310 320 330 340 350
RDLDPHAIDP YAFGLVQLLC HWLAMSSACY NPFIYAWLHD SFREELRKLL
360 370
VAWPRKIAPH GQNMTVSVVI
Length:370
Mass (Da):41,121
Last modified:November 2, 2010 - v3
Checksum:i47F71490A080C81F
GO

Sequence cautioni

The sequence AAC50504.1 differs from that shown. Reason: Frameshift at positions 168 and 175. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891R → P in AAC50504 (PubMed:8666380).Curated
Sequence conflicti194 – 1941A → P in AAC50504 (PubMed:8666380).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti283 – 2831I → V.4 Publications
Corresponds to variant rs1613448 [ dbSNP | Ensembl ].
VAR_023948
Natural varianti302 – 3021D → G.
Corresponds to variant rs8192523 [ dbSNP | Ensembl ].
VAR_029746

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32672 Genomic DNA. Translation: AAC50504.1. Frameshift.
AB015745 mRNA. Translation: BAA31159.1.
AB048946 Genomic DNA. Translation: BAB83030.1.
AL356865 Genomic DNA. Translation: CAH73066.1.
BC095539 mRNA. Translation: AAH95539.1.
BC101490 mRNA. Translation: AAI01491.1.
BC101492 mRNA. Translation: AAI01493.1.
CCDSiCCDS7606.1.
RefSeqiNP_004239.1. NM_004248.2.
UniGeneiHs.248119.

Genome annotation databases

EnsembliENST00000239032; ENSP00000239032; ENSG00000119973.
GeneIDi2834.
KEGGihsa:2834.
UCSCiuc001ldp.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32672 Genomic DNA. Translation: AAC50504.1. Frameshift.
AB015745 mRNA. Translation: BAA31159.1.
AB048946 Genomic DNA. Translation: BAB83030.1.
AL356865 Genomic DNA. Translation: CAH73066.1.
BC095539 mRNA. Translation: AAH95539.1.
BC101490 mRNA. Translation: AAI01491.1.
BC101492 mRNA. Translation: AAI01493.1.
CCDSiCCDS7606.1.
RefSeqiNP_004239.1. NM_004248.2.
UniGeneiHs.248119.

3D structure databases

ProteinModelPortaliP49683.
SMRiP49683. Positions 67-347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109095. 3 interactions.
IntActiP49683. 3 interactions.
MINTiMINT-1785715.
STRINGi9606.ENSP00000239032.

Chemistry

ChEMBLiCHEMBL1681611.
GuidetoPHARMACOLOGYi337.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP49683.

Polymorphism and mutation databases

BioMutaiPRLHR.
DMDMi311033415.

Proteomic databases

PaxDbiP49683.
PRIDEiP49683.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000239032; ENSP00000239032; ENSG00000119973.
GeneIDi2834.
KEGGihsa:2834.
UCSCiuc001ldp.2. human.

Organism-specific databases

CTDi2834.
GeneCardsiPRLHR.
H-InvDBHIX0170463.
HGNCiHGNC:4464. PRLHR.
HPAiHPA039361.
MIMi600895. gene.
neXtProtiNX_P49683.
PharmGKBiPA28847.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118781.
HOGENOMiHOG000050229.
HOVERGENiHBG101109.
InParanoidiP49683.
KOiK04314.
OMAiFIYAWLH.
OrthoDBiEOG75B85N.
PhylomeDBiP49683.
TreeFamiTF315303.

Enzyme and pathway databases

ReactomeiR-HSA-375276. Peptide ligand-binding receptors.
SignaLinkiP49683.

Miscellaneous databases

GeneWikiiProlactin-releasing_peptide_receptor.
GenomeRNAii2834.
NextBioi11185.
PROiP49683.
SOURCEiSearch...

Gene expression databases

BgeeiP49683.
CleanExiHS_PRLHR.
GenevisibleiP49683. HS.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001402. Prolrel_pep_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01018. PRPRECEPTOR.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and chromosomal mapping of three novel genes, GPR9, GPR10, and GPR14, encoding receptors related to interleukin 8, neuropeptide Y, and somatostatin receptors."
    Marchese A., Heiber M., Nguyen T., Heng H.H.Q., Saldivia V.R., Cheng R., Murphy P.M., Tsui L.-C., Shi X., Gregor P., George S.R., O'Dowd B.F., Docherty J.M.
    Genomics 29:335-344(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-283.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-283.
  3. "Transcriptional regulation of the human PRL-releasing peptide (PrRP) receptor gene by a dopamine 2 receptor agonist: cloning and characterization of the human PrRP receptor gene and its promoter region."
    Ozawa A., Yamada M., Satoh T., Monden T., Hashimoto K., Kohga H., Hashiba Y., Sasaki T., Mori M.
    Mol. Endocrinol. 16:785-798(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION, VARIANT VAL-283.
  4. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-283.
  6. Cited for: TISSUE SPECIFICITY.
  7. "The carboxyl terminus of the prolactin-releasing peptide receptor interacts with PDZ domain proteins involved in alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor clustering."
    Lin S.H.S., Arai A.C., Wang Z., Nothacker H.-P., Civelli O.
    Mol. Pharmacol. 60:916-923(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRIP1; GRIP2 AND PICK1, MUTAGENESIS OF THR-365; VAL-366; SER-367; VAL-368; VAL-369 AND ILE-370.

Entry informationi

Entry nameiPRLHR_HUMAN
AccessioniPrimary (citable) accession number: P49683
Secondary accession number(s): O75194, Q502U8, Q5VXR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 2, 2010
Last modified: May 11, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.