P49679 (IAA4_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Auxin-induced protein IAA4

Gene names

Name:

IAA4/5

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression By similarity.
Subunit structure	Homo- and heterodimers By similarity.
Subcellular location	Nucleus.
Induction	By auxin.
Domain	The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV By similarity.
Post-translational modification	Phosphorylated by phytochrome A in vitro. Ref.2
Sequence similarities	Belongs to the Aux/IAA family. Contains 1 Aux/IAA-ARF domain.
Sequence caution	The sequence CAA48298.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Auxin signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Repressor
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	auxin mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IAA1	P49677	3	EBI-632357,EBI-630505	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	Auxin-induced protein IAA4		PRO_0000112861
Regions
	Domain	93 – 180	88	Aux/IAA-ARF
	Motif	8 – 12	5	EAR-like (transcriptional repression)

Sequences

	Sequence		Length	Mass (Da)
	P49679 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: BC699EF97443E580	FASTA	189	21,036
	10 20 30 40 50 60 MEFKATELRL GLPGITEEEE KKIIHGSSVV KNNNKRQLPQ TSEESVSISK VTNDEHIVES 70 80 90 100 110 120 SSAAPPAKAK IVGWPPIRSY RKNSLHEADV GGIFVKVSMD GAPYLRKIDL RVYGGYSELL 130 140 150 160 170 180 KALETMFKLT IGEYSEREGY KGSEYAPTYE DKDGDWMLVG DVPWDMFVTS CKRLRIMKGT EAKGLGCGV

References

[1]	"Structural characterization of the early indoleacetic acid-inducible genes, PS-IAA4/5 and PS-IAA6, of pea (Pisum sativum L.)." Oeller P.W., Keller J.A., Parks J.E., Silbert J.E., Theologis A. J. Mol. Biol. 233:789-798(1993) [PubMed: 8411182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Alaska.
[2]	"Aux/IAA proteins are phosphorylated by phytochrome in vitro." Colon-Carmona A., Chen D.L., Yeh K.-C., Abel S. Plant Physiol. 124:1728-1738(2000) [PubMed: 11115889] [Abstract] Cited for: PHOSPHORYLATION BY PHYTOCHROME A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68215 mRNA. Translation: CAA48297.1. X68216 Genomic DNA. Translation: CAA48298.1. Sequence problems.
PIR	S39075.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P49679. 3 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR003311. AUX_IAA. IPR011525. AuxIAA_ARF_dimer. [Graphical view]
Pfam	PF02309. AUX_IAA. 1 hit. [Graphical view]
PROSITE	PS50962. IAA_ARF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IAA4_PEA

Accession

Primary (citable) accession number: P49679

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program