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P49677

- IAA1_ARATH

UniProt

P49677 - IAA1_ARATH

Protein

Auxin-responsive protein IAA1

Gene

IAA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression.1 Publication

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct
    3. sequence-specific DNA binding transcription factor activity Source: TAIR

    GO - Biological processi

    1. auxin-activated signaling pathway Source: UniProtKB-KW
    2. response to auxin Source: TAIR
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Auxin signaling pathway, Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Auxin-responsive protein IAA1
    Alternative name(s):
    Indoleacetic acid-induced protein 1
    Gene namesi
    Name:IAA1
    Ordered Locus Names:At4g14560
    ORF Names:dl3320w, FCAALL.409
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G14560.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 168168Auxin-responsive protein IAA1PRO_0000112832Add
    BLAST

    Post-translational modificationi

    Phosphorylated by phytochrome A in vitro.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP49677.

    Expressioni

    Tissue specificityi

    Preferentially expressed in stems, leaves and flowers.1 Publication

    Inductioni

    By auxin.1 Publication

    Gene expression databases

    ArrayExpressiP49677.
    GenevestigatoriP49677.

    Interactioni

    Subunit structurei

    Homodimers and heterodimers. Interacts with the auxin-responsive protein IAA2. Interacts with TPL.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself5EBI-630505,EBI-630505
    ARF5P930244EBI-630505,EBI-629519
    ARF7P930223EBI-630505,EBI-632284
    IAA16O244073EBI-630505,EBI-632231
    IAA18O244083EBI-630505,EBI-2295525
    IAA2P496783EBI-630505,EBI-632343
    IAA3Q388223EBI-630505,EBI-307174
    IAA4/5P496793EBI-630505,EBI-632357From a different organism.

    Protein-protein interaction databases

    BioGridi12400. 12 interactions.
    DIPiDIP-34629N.
    IntActiP49677. 32 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP49677.
    SMRiP49677. Positions 76-164.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini75 – 16288Aux/IAA-ARFPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi14 – 185EAR-like (transcriptional repression)

    Domaini

    The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV.

    Sequence similaritiesi

    Belongs to the Aux/IAA family.Curated
    Contains 1 Aux/IAA-ARF domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG329801.
    HOGENOMiHOG000238261.
    InParanoidiP49677.
    KOiK14484.
    OMAiCQKLRIM.
    PhylomeDBiP49677.

    Family and domain databases

    InterProiIPR003311. AUX_IAA.
    IPR011525. AuxIAA_ARF_dimer.
    [Graphical view]
    PfamiPF02309. AUX_IAA. 1 hit.
    [Graphical view]
    PROSITEiPS50962. IAA_ARF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P49677-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVTNGLNLK DTELRLGLPG AQEEQQLELS CVRSNNKRKN NDSTEESAPP    50
    PAKTQIVGWP PVRSNRKNNN NKNVSYVKVS MDGAPYLRKI DLKMYKNYPE 100
    LLKALENMFK FTVGEYSERE GYKGSGFVPT YEDKDGDWML VGDVPWDMFS 150
    SSCQKLRIMK GSEAPTAL 168
    Length:168
    Mass (Da):19,032
    Last modified:December 1, 2000 - v2
    Checksum:i17CA9B3BEB7962EE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti52 – 521A → T in AAA16569. (PubMed:8278386)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L15448 mRNA. Translation: AAA16569.1.
    Z97336 Genomic DNA. Translation: CAB10235.1.
    AL161539 Genomic DNA. Translation: CAB78498.1.
    CP002687 Genomic DNA. Translation: AEE83460.1.
    AY133714 mRNA. Translation: AAM91648.1.
    PIRiA71408.
    RefSeqiNP_193192.1. NM_117536.3.
    UniGeneiAt.43941.

    Genome annotation databases

    EnsemblPlantsiAT4G14560.1; AT4G14560.1; AT4G14560.
    GeneIDi827103.
    KEGGiath:AT4G14560.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L15448 mRNA. Translation: AAA16569.1 .
    Z97336 Genomic DNA. Translation: CAB10235.1 .
    AL161539 Genomic DNA. Translation: CAB78498.1 .
    CP002687 Genomic DNA. Translation: AEE83460.1 .
    AY133714 mRNA. Translation: AAM91648.1 .
    PIRi A71408.
    RefSeqi NP_193192.1. NM_117536.3.
    UniGenei At.43941.

    3D structure databases

    ProteinModelPortali P49677.
    SMRi P49677. Positions 76-164.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 12400. 12 interactions.
    DIPi DIP-34629N.
    IntActi P49677. 32 interactions.

    Proteomic databases

    PRIDEi P49677.

    Protocols and materials databases

    DNASUi 827103.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G14560.1 ; AT4G14560.1 ; AT4G14560 .
    GeneIDi 827103.
    KEGGi ath:AT4G14560.

    Organism-specific databases

    GeneFarmi 3140. 342.
    TAIRi AT4G14560.

    Phylogenomic databases

    eggNOGi NOG329801.
    HOGENOMi HOG000238261.
    InParanoidi P49677.
    KOi K14484.
    OMAi CQKLRIM.
    PhylomeDBi P49677.

    Gene expression databases

    ArrayExpressi P49677.
    Genevestigatori P49677.

    Family and domain databases

    InterProi IPR003311. AUX_IAA.
    IPR011525. AuxIAA_ARF_dimer.
    [Graphical view ]
    Pfami PF02309. AUX_IAA. 1 hit.
    [Graphical view ]
    PROSITEi PS50962. IAA_ARF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Early auxin-induced genes encode short-lived nuclear proteins."
      Abel S., Oeller P.W., Theologis A.
      Proc. Natl. Acad. Sci. U.S.A. 91:326-330(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana."
      Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.
      , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.
      Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana."
      Abel S., Nguyen M.D., Theologis A.
      J. Mol. Biol. 251:533-549(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
    7. "Protein-protein interactions among the Aux/IAA proteins."
      Kim J., Harter K., Theologis A.
      Proc. Natl. Acad. Sci. U.S.A. 94:11786-11791(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: DIMERIZATION.
    8. "Aux/IAA proteins are phosphorylated by phytochrome in vitro."
      Colon-Carmona A., Chen D.L., Yeh K.-C., Abel S.
      Plant Physiol. 124:1728-1738(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY PHYTOCHROME A.
    9. "Genetics of Aux/IAA and ARF action in plant growth and development."
      Liscum E., Reed J.W.
      Plant Mol. Biol. 49:387-400(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
    10. "Aux/IAA proteins contain a potent transcriptional repression domain."
      Tiwari S.B., Hagen G., Guilfoyle T.J.
      Plant Cell 16:533-543(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TRANSCRIPTIONAL REPRESSION DOMAIN.
    11. "TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis."
      Szemenyei H., Hannon M., Long J.A.
      Science 319:1384-1386(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TPL.

    Entry informationi

    Entry nameiIAA1_ARATH
    AccessioniPrimary (citable) accession number: P49677
    Secondary accession number(s): O23312
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3