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Protein

Auxin-responsive protein IAA1

Gene

IAA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression.1 Publication

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR
  • transcription regulatory region DNA binding Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • response to auxin Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionRepressor
Biological processAuxin signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Auxin-responsive protein IAA1
Alternative name(s):
Indoleacetic acid-induced protein 1
Gene namesi
Name:IAA1
Ordered Locus Names:At4g14560
ORF Names:dl3320w, FCAALL.409
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G14560.
TAIRilocus:2129910. AT4G14560.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001128321 – 168Auxin-responsive protein IAA1Add BLAST168

Post-translational modificationi

Phosphorylated by phytochrome A in vitro.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49677.

Expressioni

Tissue specificityi

Preferentially expressed in stems, leaves and flowers.1 Publication

Inductioni

By auxin.1 Publication

Gene expression databases

ExpressionAtlasiP49677. baseline and differential.
GenevisibleiP49677. AT.

Interactioni

Subunit structurei

Homodimers and heterodimers. Interacts with the auxin-responsive protein IAA2. Interacts with TPL.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi12400. 36 interactors.
DIPiDIP-34629N.
ELMiP49677.
IntActiP49677. 41 interactors.
STRINGi3702.AT4G14560.1.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5C7FX-ray2.70E/F/G/H10-20[»]
ProteinModelPortaliP49677.
SMRiP49677.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 161PB1PROSITE-ProRule annotationAdd BLAST88

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi14 – 18EAR-like (transcriptional repression)5

Domaini

The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV.

Sequence similaritiesi

Belongs to the Aux/IAA family.Curated

Phylogenomic databases

HOGENOMiHOG000238261.
InParanoidiP49677.
KOiK14484.
OMAiENMFKCS.
OrthoDBiEOG09360PSB.
PhylomeDBiP49677.

Family and domain databases

InterProiView protein in InterPro
IPR033389. AUX/IAA_dom.
IPR003311. AUX_IAA.
IPR000270. PB1_dom.
PANTHERiPTHR31734. PTHR31734. 1 hit.
PfamiView protein in Pfam
PF02309. AUX_IAA. 1 hit.
PROSITEiView protein in PROSITE
PS51745. PB1. 1 hit.

Sequencei

Sequence statusi: Complete.

P49677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVTNGLNLK DTELRLGLPG AQEEQQLELS CVRSNNKRKN NDSTEESAPP
60 70 80 90 100
PAKTQIVGWP PVRSNRKNNN NKNVSYVKVS MDGAPYLRKI DLKMYKNYPE
110 120 130 140 150
LLKALENMFK FTVGEYSERE GYKGSGFVPT YEDKDGDWML VGDVPWDMFS
160
SSCQKLRIMK GSEAPTAL
Length:168
Mass (Da):19,032
Last modified:December 1, 2000 - v2
Checksum:i17CA9B3BEB7962EE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52A → T in AAA16569 (PubMed:8278386).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15448 mRNA. Translation: AAA16569.1.
Z97336 Genomic DNA. Translation: CAB10235.1.
AL161539 Genomic DNA. Translation: CAB78498.1.
CP002687 Genomic DNA. Translation: AEE83460.1.
AY133714 mRNA. Translation: AAM91648.1.
PIRiA71408.
RefSeqiNP_193192.1. NM_117536.4.
UniGeneiAt.43941.

Genome annotation databases

EnsemblPlantsiAT4G14560.1; AT4G14560.1; AT4G14560.
GeneIDi827103.
GrameneiAT4G14560.1; AT4G14560.1; AT4G14560.
KEGGiath:AT4G14560.

Similar proteinsi

Entry informationi

Entry nameiIAA1_ARATH
AccessioniPrimary (citable) accession number: P49677
Secondary accession number(s): O23312
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 1, 2000
Last modified: September 27, 2017
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families