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P49677 (IAA1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Auxin-responsive protein IAA1
Alternative name(s):
Indoleacetic acid-induced protein 1
Gene names
Name:IAA1
Ordered Locus Names:At4g14560
ORF Names:dl3320w, FCAALL.409
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length168 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression. Ref.9

Subunit structure

Homodimers and heterodimers. Interacts with the auxin-responsive protein IAA2. Interacts with TPL. Ref.7 Ref.11

Subcellular location

Nucleus.

Tissue specificity

Preferentially expressed in stems, leaves and flowers. Ref.6

Induction

By auxin. Ref.6

Domain

The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV. Ref.10

Post-translational modification

Phosphorylated by phytochrome A in vitro. Ref.8

Sequence similarities

Belongs to the Aux/IAA family.

Contains 1 Aux/IAA-ARF domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 168168Auxin-responsive protein IAA1
PRO_0000112832

Regions

Domain75 – 16288Aux/IAA-ARF
Motif14 – 185EAR-like (transcriptional repression)

Experimental info

Sequence conflict521A → T in AAA16569. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P49677 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 17CA9B3BEB7962EE

FASTA16819,032
        10         20         30         40         50         60 
MEVTNGLNLK DTELRLGLPG AQEEQQLELS CVRSNNKRKN NDSTEESAPP PAKTQIVGWP 

        70         80         90        100        110        120 
PVRSNRKNNN NKNVSYVKVS MDGAPYLRKI DLKMYKNYPE LLKALENMFK FTVGEYSERE 

       130        140        150        160 
GYKGSGFVPT YEDKDGDWML VGDVPWDMFS SSCQKLRIMK GSEAPTAL 

« Hide

References

« Hide 'large scale' references
[1]"Early auxin-induced genes encode short-lived nuclear proteins."
Abel S., Oeller P.W., Theologis A.
Proc. Natl. Acad. Sci. U.S.A. 91:326-330(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana."
Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. expand/collapse author list , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.
Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana."
Abel S., Nguyen M.D., Theologis A.
J. Mol. Biol. 251:533-549(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[7]"Protein-protein interactions among the Aux/IAA proteins."
Kim J., Harter K., Theologis A.
Proc. Natl. Acad. Sci. U.S.A. 94:11786-11791(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: DIMERIZATION.
[8]"Aux/IAA proteins are phosphorylated by phytochrome in vitro."
Colon-Carmona A., Chen D.L., Yeh K.-C., Abel S.
Plant Physiol. 124:1728-1738(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY PHYTOCHROME A.
[9]"Genetics of Aux/IAA and ARF action in plant growth and development."
Liscum E., Reed J.W.
Plant Mol. Biol. 49:387-400(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
[10]"Aux/IAA proteins contain a potent transcriptional repression domain."
Tiwari S.B., Hagen G., Guilfoyle T.J.
Plant Cell 16:533-543(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TRANSCRIPTIONAL REPRESSION DOMAIN.
[11]"TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis."
Szemenyei H., Hannon M., Long J.A.
Science 319:1384-1386(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TPL.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L15448 mRNA. Translation: AAA16569.1.
Z97336 Genomic DNA. Translation: CAB10235.1.
AL161539 Genomic DNA. Translation: CAB78498.1.
CP002687 Genomic DNA. Translation: AEE83460.1.
AY133714 mRNA. Translation: AAM91648.1.
PIRA71408.
RefSeqNP_193192.1. NM_117536.3.
UniGeneAt.43941.

3D structure databases

ProteinModelPortalP49677.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid12400. 12 interactions.
IntActP49677. 32 interactions.

Proteomic databases

PRIDEP49677.

Protocols and materials databases

DNASU827103.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G14560.1; AT4G14560.1; AT4G14560.
GeneID827103.
KEGGath:AT4G14560.

Organism-specific databases

GeneFarm3140. 342.
TAIRAT4G14560.

Phylogenomic databases

eggNOGNOG329801.
HOGENOMHOG000238261.
InParanoidP49677.
KOK14484.
OMACQKLRIM.
PhylomeDBP49677.
ProtClustDBCLSN2681778.

Gene expression databases

ArrayExpressP49677.
GenevestigatorP49677.

Family and domain databases

InterProIPR003311. AUX_IAA.
IPR011525. AuxIAA_ARF_dimer.
[Graphical view]
PfamPF02309. AUX_IAA. 1 hit.
[Graphical view]
PROSITEPS50962. IAA_ARF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIAA1_ARATH
AccessionPrimary (citable) accession number: P49677
Secondary accession number(s): O23312
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 1, 2000
Last modified: April 16, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names