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P49676 (BGAL_BRAOL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Lactase
EC=3.2.1.23
OrganismBrassica oleracea (Wild cabbage)
Taxonomic identifier3712 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length828 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Contains 1 SUEL-type lectin domain.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 828808Beta-galactosidase
PRO_0000012194

Regions

Domain742 – 82887SUEL-type lectin

Sites

Active site1831Proton donor Potential
Active site2521Nucleophile Potential

Amino acid modifications

Glycosylation231N-linked (GlcNAc...) Potential
Glycosylation1531N-linked (GlcNAc...) Potential
Glycosylation2531N-linked (GlcNAc...) Potential
Glycosylation3501N-linked (GlcNAc...) Potential
Glycosylation3791N-linked (GlcNAc...) Potential
Glycosylation4921N-linked (GlcNAc...) Potential
Glycosylation6671N-linked (GlcNAc...) Potential
Glycosylation7991N-linked (GlcNAc...) Potential
Glycosylation8031N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P49676 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 45840C0645C72AEF

FASTA82893,019
        10         20         30         40         50         60 
MKMKQFNLLS LFLILITSFG SANSTIVSHD ERAITIDGQR RILLSGSIHY PRSTSDMWPD 

        70         80         90        100        110        120 
LISKAKDGGL DTIETYVFWN AHEPSRRQYD FSGNLDLVRF IKTIQSAGLY SVLRIGPYVC 

       130        140        150        160        170        180 
AEWNYGGFPV WLHNMPDMKF RTINPGFMNE MQNFTTKIVN MMKEESLFAS QGGPIILAQI 

       190        200        210        220        230        240 
ENEYGNVISS YGAEGKAYID WCANMANSLD IGVPWIMCQQ PHAPQPMIET CNGFYCDQYK 

       250        260        270        280        290        300 
PSNPSSPKMW TENWTGWFKN WGGKHPYRTA EDLAFSVARF FQTGGTFQNY YMYHGGTNFG 

       310        320        330        340        350        360 
RVAGGPYITT SYDYDAPLDE YGNLNQPKWG HLKQLHTLLK SMEKPLTYGN ISTIDLGNSV 

       370        380        390        400        410        420 
TATVYSTNEK SSCFIGNVNA TADALVNFKG KDYNVPAWSV SVLPDCDKEA YNTARVNTQT 

       430        440        450        460        470        480 
SIITEDSCDE PEKLKWTWRP EFTTQKTILK GSGDLIAKGL VDQKDVTNDA SDYLWYMTRV 

       490        500        510        520        530        540 
HLDKKDPIWS RNMSLRVHSN AHVLHAYVNG KYVGNQIVRD NKFDYRFEKK VNLVHGTNHL 

       550        560        570        580        590        600 
ALLSVSVGLQ NYGPFFESGP TGINGPVKLV GYKGDETIEK DLSKHQWDYK IGLNGFNHKL 

       610        620        630        640        650        660 
FSMKSAGHHH RKWSTEKLPA DRMLSWYKAN FKAPLGKDPV IVDLNGLGKG EVWINGQSIG 

       670        680        690        700        710        720 
RYWPSFNSSD EGCTEECDYR GEYGSDKCAF MCGKPTQRWY HVPRSFLNDK GHNTITLFEE 

       730        740        750        760        770        780 
MGGDPSMVKF KTVVTGRVCA KAHEHNKVEL SCNNRPISAV KFASFGNPSG QCGSFAAGSC 

       790        800        810        820 
EGAKDAVKVV AKECVGKLNC TMNVSSHKFG SNLDCGDSPK RLFVEVEC 

« Hide

References

[1]"A beta-galactosidase cDNA homolog from broccoli (Brassica oleracea L.)."
Downs C.G., Almira E.C.
Plant Gene Register PGR95-017
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Shogun.
Tissue: Floret.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X84684 mRNA. Translation: CAA59162.1.
PIRS52393.

3D structure databases

ProteinModelPortalP49676.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_BRAOL
AccessionPrimary (citable) accession number: P49676
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries