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P49676

- BGAL_BRAOL

UniProt

P49676 - BGAL_BRAOL

Protein

Beta-galactosidase

Gene
N/A
Organism
Brassica oleracea (Wild cabbage)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei183 – 1831Proton donorSequence Analysis
    Active sitei252 – 2521NucleophileSequence Analysis

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH35. Glycoside Hydrolase Family 35.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase (EC:3.2.1.23)
    Short name:
    Lactase
    OrganismiBrassica oleracea (Wild cabbage)
    Taxonomic identifieri3712 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 828808Beta-galactosidasePRO_0000012194Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi350 – 3501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi379 – 3791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi667 – 6671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi799 – 7991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi803 – 8031N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliP49676.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini742 – 82887SUEL-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated
    Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    IPR000922. Lectin_gal-bd_dom.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF02140. Gal_Lectin. 1 hit.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PRINTSiPR00742. GLHYDRLASE35.
    SUPFAMiSSF49785. SSF49785. 3 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    PS50228. SUEL_LECTIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49676-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKMKQFNLLS LFLILITSFG SANSTIVSHD ERAITIDGQR RILLSGSIHY    50
    PRSTSDMWPD LISKAKDGGL DTIETYVFWN AHEPSRRQYD FSGNLDLVRF 100
    IKTIQSAGLY SVLRIGPYVC AEWNYGGFPV WLHNMPDMKF RTINPGFMNE 150
    MQNFTTKIVN MMKEESLFAS QGGPIILAQI ENEYGNVISS YGAEGKAYID 200
    WCANMANSLD IGVPWIMCQQ PHAPQPMIET CNGFYCDQYK PSNPSSPKMW 250
    TENWTGWFKN WGGKHPYRTA EDLAFSVARF FQTGGTFQNY YMYHGGTNFG 300
    RVAGGPYITT SYDYDAPLDE YGNLNQPKWG HLKQLHTLLK SMEKPLTYGN 350
    ISTIDLGNSV TATVYSTNEK SSCFIGNVNA TADALVNFKG KDYNVPAWSV 400
    SVLPDCDKEA YNTARVNTQT SIITEDSCDE PEKLKWTWRP EFTTQKTILK 450
    GSGDLIAKGL VDQKDVTNDA SDYLWYMTRV HLDKKDPIWS RNMSLRVHSN 500
    AHVLHAYVNG KYVGNQIVRD NKFDYRFEKK VNLVHGTNHL ALLSVSVGLQ 550
    NYGPFFESGP TGINGPVKLV GYKGDETIEK DLSKHQWDYK IGLNGFNHKL 600
    FSMKSAGHHH RKWSTEKLPA DRMLSWYKAN FKAPLGKDPV IVDLNGLGKG 650
    EVWINGQSIG RYWPSFNSSD EGCTEECDYR GEYGSDKCAF MCGKPTQRWY 700
    HVPRSFLNDK GHNTITLFEE MGGDPSMVKF KTVVTGRVCA KAHEHNKVEL 750
    SCNNRPISAV KFASFGNPSG QCGSFAAGSC EGAKDAVKVV AKECVGKLNC 800
    TMNVSSHKFG SNLDCGDSPK RLFVEVEC 828
    Length:828
    Mass (Da):93,019
    Last modified:February 1, 1996 - v1
    Checksum:i45840C0645C72AEF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84684 mRNA. Translation: CAA59162.1.
    PIRiS52393.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84684 mRNA. Translation: CAA59162.1 .
    PIRi S52393.

    3D structure databases

    ProteinModelPortali P49676.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH35. Glycoside Hydrolase Family 35.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    IPR000922. Lectin_gal-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF02140. Gal_Lectin. 1 hit.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PRINTSi PR00742. GLHYDRLASE35.
    SUPFAMi SSF49785. SSF49785. 3 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    PS50228. SUEL_LECTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A beta-galactosidase cDNA homolog from broccoli (Brassica oleracea L.)."
      Downs C.G., Almira E.C.
      Plant Gene Register PGR95-017
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Shogun.
      Tissue: Floret.

    Entry informationi

    Entry nameiBGAL_BRAOL
    AccessioniPrimary (citable) accession number: P49676
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3