Reviewed, UniProtKB/Swiss-Prot P49676 (BGAL_BRAOL)

Names and origin

Protein names	Recommended name:     Beta-galactosidase       Short name=Lactase     EC=3.2.1.23
Organism	Brassica oleracea (Wild cabbage)
Taxonomic identifier	3712 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family. Contains 1 SUEL-type lectin domain.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 828	808	Beta-galactosidase		PRO_0000012194
Regions
	Domain	742 – 828	87	SUEL-type lectin
Sites
	Active site	183	1	Proton donor Potential
	Active site	252	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	253	1	N-linked (GlcNAc...) Potential
	Glycosylation	350	1	N-linked (GlcNAc...) Potential
	Glycosylation	379	1	N-linked (GlcNAc...) Potential
	Glycosylation	492	1	N-linked (GlcNAc...) Potential
	Glycosylation	667	1	N-linked (GlcNAc...) Potential
	Glycosylation	799	1	N-linked (GlcNAc...) Potential
	Glycosylation	803	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P49676-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 45840C0645C72AEF	FASTA	828	93,019
	10 20 30 40 50 60 MKMKQFNLLS LFLILITSFG SANSTIVSHD ERAITIDGQR RILLSGSIHY PRSTSDMWPD 70 80 90 100 110 120 LISKAKDGGL DTIETYVFWN AHEPSRRQYD FSGNLDLVRF IKTIQSAGLY SVLRIGPYVC 130 140 150 160 170 180 AEWNYGGFPV WLHNMPDMKF RTINPGFMNE MQNFTTKIVN MMKEESLFAS QGGPIILAQI 190 200 210 220 230 240 ENEYGNVISS YGAEGKAYID WCANMANSLD IGVPWIMCQQ PHAPQPMIET CNGFYCDQYK 250 260 270 280 290 300 PSNPSSPKMW TENWTGWFKN WGGKHPYRTA EDLAFSVARF FQTGGTFQNY YMYHGGTNFG 310 320 330 340 350 360 RVAGGPYITT SYDYDAPLDE YGNLNQPKWG HLKQLHTLLK SMEKPLTYGN ISTIDLGNSV 370 380 390 400 410 420 TATVYSTNEK SSCFIGNVNA TADALVNFKG KDYNVPAWSV SVLPDCDKEA YNTARVNTQT 430 440 450 460 470 480 SIITEDSCDE PEKLKWTWRP EFTTQKTILK GSGDLIAKGL VDQKDVTNDA SDYLWYMTRV 490 500 510 520 530 540 HLDKKDPIWS RNMSLRVHSN AHVLHAYVNG KYVGNQIVRD NKFDYRFEKK VNLVHGTNHL 550 560 570 580 590 600 ALLSVSVGLQ NYGPFFESGP TGINGPVKLV GYKGDETIEK DLSKHQWDYK IGLNGFNHKL 610 620 630 640 650 660 FSMKSAGHHH RKWSTEKLPA DRMLSWYKAN FKAPLGKDPV IVDLNGLGKG EVWINGQSIG 670 680 690 700 710 720 RYWPSFNSSD EGCTEECDYR GEYGSDKCAF MCGKPTQRWY HVPRSFLNDK GHNTITLFEE 730 740 750 760 770 780 MGGDPSMVKF KTVVTGRVCA KAHEHNKVEL SCNNRPISAV KFASFGNPSG QCGSFAAGSC 790 800 810 820 EGAKDAVKVV AKECVGKLNC TMNVSSHKFG SNLDCGDSPK RLFVEVEC

References

[1]	"A beta-galactosidase cDNA homolog from broccoli (Brassica oleracea L.)." Downs C.G., Almira E.C. Plant Gene Register PGR95-017 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Shogun. Tissue: Floret.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X84684 mRNA. Translation: CAA59162.1.
PIR	S52393.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Enzyme and pathway databases
BRENDA	3.2.1.23. 1738.
Family and domain databases
InterPro	IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_sg_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR000922. Lectin_gal_bd. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF02140. Gal_Lectin. 1 hit. PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
ProDom	PD005612. Gal_lectin. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. PS50228. SUEL_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_BRAOL

Accession

Primary (citable) accession number: P49676

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	September 22, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)