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P49676

- BGAL_BRAOL

UniProt

P49676 - BGAL_BRAOL

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Protein

Beta-galactosidase

Gene
N/A
Organism
Brassica oleracea (Wild cabbage)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei183 – 1831Proton donorSequence Analysis
Active sitei252 – 2521NucleophileSequence Analysis

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Lactase
OrganismiBrassica oleracea (Wild cabbage)
Taxonomic identifieri3712 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 828808Beta-galactosidasePRO_0000012194Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi350 – 3501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi379 – 3791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi667 – 6671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi799 – 7991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi803 – 8031N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliP49676.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini742 – 82887SUEL-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated
Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49676 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKMKQFNLLS LFLILITSFG SANSTIVSHD ERAITIDGQR RILLSGSIHY
60 70 80 90 100
PRSTSDMWPD LISKAKDGGL DTIETYVFWN AHEPSRRQYD FSGNLDLVRF
110 120 130 140 150
IKTIQSAGLY SVLRIGPYVC AEWNYGGFPV WLHNMPDMKF RTINPGFMNE
160 170 180 190 200
MQNFTTKIVN MMKEESLFAS QGGPIILAQI ENEYGNVISS YGAEGKAYID
210 220 230 240 250
WCANMANSLD IGVPWIMCQQ PHAPQPMIET CNGFYCDQYK PSNPSSPKMW
260 270 280 290 300
TENWTGWFKN WGGKHPYRTA EDLAFSVARF FQTGGTFQNY YMYHGGTNFG
310 320 330 340 350
RVAGGPYITT SYDYDAPLDE YGNLNQPKWG HLKQLHTLLK SMEKPLTYGN
360 370 380 390 400
ISTIDLGNSV TATVYSTNEK SSCFIGNVNA TADALVNFKG KDYNVPAWSV
410 420 430 440 450
SVLPDCDKEA YNTARVNTQT SIITEDSCDE PEKLKWTWRP EFTTQKTILK
460 470 480 490 500
GSGDLIAKGL VDQKDVTNDA SDYLWYMTRV HLDKKDPIWS RNMSLRVHSN
510 520 530 540 550
AHVLHAYVNG KYVGNQIVRD NKFDYRFEKK VNLVHGTNHL ALLSVSVGLQ
560 570 580 590 600
NYGPFFESGP TGINGPVKLV GYKGDETIEK DLSKHQWDYK IGLNGFNHKL
610 620 630 640 650
FSMKSAGHHH RKWSTEKLPA DRMLSWYKAN FKAPLGKDPV IVDLNGLGKG
660 670 680 690 700
EVWINGQSIG RYWPSFNSSD EGCTEECDYR GEYGSDKCAF MCGKPTQRWY
710 720 730 740 750
HVPRSFLNDK GHNTITLFEE MGGDPSMVKF KTVVTGRVCA KAHEHNKVEL
760 770 780 790 800
SCNNRPISAV KFASFGNPSG QCGSFAAGSC EGAKDAVKVV AKECVGKLNC
810 820
TMNVSSHKFG SNLDCGDSPK RLFVEVEC
Length:828
Mass (Da):93,019
Last modified:February 1, 1996 - v1
Checksum:i45840C0645C72AEF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84684 mRNA. Translation: CAA59162.1.
PIRiS52393.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84684 mRNA. Translation: CAA59162.1 .
PIRi S52393.

3D structure databases

ProteinModelPortali P49676.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SUPFAMi SSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A beta-galactosidase cDNA homolog from broccoli (Brassica oleracea L.)."
    Downs C.G., Almira E.C.
    Plant Gene Register PGR95-017
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Shogun.
    Tissue: Floret.

Entry informationi

Entry nameiBGAL_BRAOL
AccessioniPrimary (citable) accession number: P49676
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3