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P49676

- BGAL_BRAOL

UniProt

P49676 - BGAL_BRAOL

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Protein

Beta-galactosidase

Gene
N/A
Organism
Brassica oleracea (Wild cabbage)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei183 – 1831Proton donor Reviewed prediction
Active sitei252 – 2521Nucleophile Reviewed prediction

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Lactase
OrganismiBrassica oleracea (Wild cabbage)
Taxonomic identifieri3712 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

Secretedextracellular spaceapoplast Reviewed prediction

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed predictionAdd
BLAST
Chaini21 – 828808Beta-galactosidasePRO_0000012194Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi23 – 231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi153 – 1531N-linked (GlcNAc...) Reviewed prediction
Glycosylationi253 – 2531N-linked (GlcNAc...) Reviewed prediction
Glycosylationi350 – 3501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi379 – 3791N-linked (GlcNAc...) Reviewed prediction
Glycosylationi492 – 4921N-linked (GlcNAc...) Reviewed prediction
Glycosylationi667 – 6671N-linked (GlcNAc...) Reviewed prediction
Glycosylationi799 – 7991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi803 – 8031N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliP49676.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini742 – 82887SUEL-type lectinAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49676-1 [UniParc]FASTAAdd to Basket

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MKMKQFNLLS LFLILITSFG SANSTIVSHD ERAITIDGQR RILLSGSIHY    50
PRSTSDMWPD LISKAKDGGL DTIETYVFWN AHEPSRRQYD FSGNLDLVRF 100
IKTIQSAGLY SVLRIGPYVC AEWNYGGFPV WLHNMPDMKF RTINPGFMNE 150
MQNFTTKIVN MMKEESLFAS QGGPIILAQI ENEYGNVISS YGAEGKAYID 200
WCANMANSLD IGVPWIMCQQ PHAPQPMIET CNGFYCDQYK PSNPSSPKMW 250
TENWTGWFKN WGGKHPYRTA EDLAFSVARF FQTGGTFQNY YMYHGGTNFG 300
RVAGGPYITT SYDYDAPLDE YGNLNQPKWG HLKQLHTLLK SMEKPLTYGN 350
ISTIDLGNSV TATVYSTNEK SSCFIGNVNA TADALVNFKG KDYNVPAWSV 400
SVLPDCDKEA YNTARVNTQT SIITEDSCDE PEKLKWTWRP EFTTQKTILK 450
GSGDLIAKGL VDQKDVTNDA SDYLWYMTRV HLDKKDPIWS RNMSLRVHSN 500
AHVLHAYVNG KYVGNQIVRD NKFDYRFEKK VNLVHGTNHL ALLSVSVGLQ 550
NYGPFFESGP TGINGPVKLV GYKGDETIEK DLSKHQWDYK IGLNGFNHKL 600
FSMKSAGHHH RKWSTEKLPA DRMLSWYKAN FKAPLGKDPV IVDLNGLGKG 650
EVWINGQSIG RYWPSFNSSD EGCTEECDYR GEYGSDKCAF MCGKPTQRWY 700
HVPRSFLNDK GHNTITLFEE MGGDPSMVKF KTVVTGRVCA KAHEHNKVEL 750
SCNNRPISAV KFASFGNPSG QCGSFAAGSC EGAKDAVKVV AKECVGKLNC 800
TMNVSSHKFG SNLDCGDSPK RLFVEVEC 828
Length:828
Mass (Da):93,019
Last modified:February 1, 1996 - v1
Checksum:i45840C0645C72AEF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84684 mRNA. Translation: CAA59162.1.
PIRiS52393.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84684 mRNA. Translation: CAA59162.1 .
PIRi S52393.

3D structure databases

ProteinModelPortali P49676.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SUPFAMi SSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A beta-galactosidase cDNA homolog from broccoli (Brassica oleracea L.)."
    Downs C.G., Almira E.C.
    Plant Gene Register PGR95-017
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Shogun.
    Tissue: Floret.

Entry informationi

Entry nameiBGAL_BRAOL
AccessioniPrimary (citable) accession number: P49676
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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