Reviewed,
UniProtKB/Swiss-Prot P49662 (CASP4_HUMAN)
Last modified
November 25, 2008.
Version 84.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Caspase-4 Short name=CASP-4 EC=3.4.22.57 Alternative name(s): ICH-2 protease TX protease ICE(rel)-II Cleaved into the following 2 chains: 1- Recommended name: Caspase-4 subunit 1 2- Recommended name: Caspase-4 subunit 2 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 377 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. |
| Catalytic activity | Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-. |
| Subunit structure | Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit By similarity. |
| Tissue specificity | Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. |
| Post-translational modification | The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8. |
| Sequence similarities | Belongs to the peptidase C14 family. Contains 1 CARD domain. |
Ontologies
Keywords | |
|---|---|
| Biological process | Apoptosis |
| Molecular function | Hydrolase Protease Thiol protease |
| PTM | Phosphoprotein Zymogen |
Gene Ontology (GO) | |
| Biological process | induction of apoptosis Ref.1 Traceable author statement. Source: ProtInc proteolysis Ref.3Traceable author statement. Source: ProtInc |
| Cellular component | intracellular Inferred from electronic annotation. Source: InterPro |
| Molecular function | cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| CDK2 | P24941 | 1 | EBI-1057327,EBI-375096 | |
| CTNNBIP1 | Q9NSA3 | 1 | EBI-1057327,EBI-747082 | |
| EIF2S3 | P41091 | 1 | EBI-1057327,EBI-1054228 | |
| GSN | P06396 | 1 | EBI-1057327,EBI-351506 | |
| HEATR2 | Q86Y56 | 1 | EBI-1057327,EBI-719966 | |
| HIGD1A | Q9Y241 | 1 | EBI-1057327,EBI-1051940 | |
| KIAA0776 | O94874 | 1 | EBI-1057327,EBI-1048088 | |
| MSH6 | P52701 | 1 | EBI-1057327,EBI-395529 | |
| MTHFD1L | Q6UB35 | 1 | EBI-1057327,EBI-1046835 | |
| NUP93 | Q8N1F7 | 1 | EBI-1057327,EBI-1042703 | |
| PSMB1 | P20618 | 1 | EBI-1057327,EBI-372273 | |
| SLC25A22 | Q9H936 | 1 | EBI-1057327,EBI-1050588 | |
| SMC1A | Q14683 | 1 | EBI-1057327,EBI-80690 | |
| SMC3 | Q9UQE7 | 1 | EBI-1057327,EBI-80718 | |
| SRPRB | Q9Y5M8 | 1 | EBI-1057327,EBI-355393 | |
| VDAC3 | Q9Y277 | 1 | EBI-1057327,EBI-354196 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Propeptide | 1 – ?80 | 80 | Potential | PRO_0000004596 | |||||
| Chain | ?81 – 270 | 190 | Caspase-4 subunit 1 | PRO_0000004597 | |||||
| Propeptide | 271 – 289 | 19 | Potential | PRO_0000004598 | |||||
| Chain | 290 – 377 | 88 | Caspase-4 subunit 2 | PRO_0000004599 | |||||
Regions | |||||||||
| Domain | 1 – 91 | 91 | CARD | ||||||
Sites | |||||||||
| Active site | 210 | 1 | By similarity | ||||||
| Active site | 258 | 1 | |||||||
Amino acid modifications | |||||||||
| Modified residue | 83 | 1 | Phosphoserine | ||||||
Experimental info | |||||||||
| Mutagenesis | 258 | 1 | C → S: Loss of activity | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "A novel human protease similar to the interleukin-1 beta converting enzyme induces apoptosis in transfected cells." Faucheu C., Diu A., Chan A.W.E., Blanchet A.-M., Miossec C., Herve F., Collard-Dutilleul V., Gu Y., Aldape R.A., Lippke J.A., Rocher C., Su M.S.-S., Livingston D.J., Hercend T., Lalanne J.-L. EMBO J. 14:1914-1922(1995) [PubMed: 7743998] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF CYS-258, 3D-STRUCTURE MODELING. Tissue: Placenta. |
| [2] | "Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases." Munday N.A., Vaillancourt J.P., Ali A., Casano F.J., Miller D.K., Molineaux S.M., Yamin T.-T., Yu V.L., Nicholson D.W. J. Biol. Chem. 270:15870-15876(1995) [PubMed: 7797592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Identification and characterization of ICH-2, a novel member of the interleukin-1 beta-converting enzyme family of cysteine proteases." Kamens J., Paskind M., Hugunin M., Talanian R.V., Allen H., Banach D., Bump N.J., Hackett M.C., Johnston C.G., Li P., Mankovich J.A., Terranova M., Ghayur T. J. Biol. Chem. 270:15250-15256(1995) [PubMed: 7797510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Tissue: Thymus. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis. |
| [5] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Z48810 mRNA. Translation: CAA88750.1. U28014 mRNA. Translation: AAA75171.1. U25804 mRNA. Translation: AAA86890.1. BC017839 mRNA. Translation: AAH17839.1. | |
| PIR | A57511. |
| RefSeq | NP_001216.1. |
| UniGene | Hs.138378 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1ICE based on UniProtKB P29466. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P49662. |
Protein family/group databases | |
| MEROPS | C14.007. |
PTM databases | |
| PhosphoSite | P49662. |
Genome annotation databases | |
| Ensembl | ENSG00000196954. Homo sapiens. [Contig view] |
| GeneID | 837. |
| KEGG | hsa:837. |
Organism-specific databases | |
| H-InvDB | HIX0010073. |
| HGNC | HGNC:1505. CASP4. |
| MIM | 602664. gene. |
| PharmGKB | PA26088. |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOGENOM | P49662. |
| HOVERGEN | P49662. |
Gene expression databases | |
| ArrayExpress | P49662. |
| CleanEx | HS_CASP4. |
| GermOnline | ENSG00000196954. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001315. CARD. IPR017350. Caspase_IL-1_beta. IPR011029. DEATH_like. IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR016129. Pept_C14_ICE_p20_AS. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view] |
| Gene3D | G3DSA:1.10.533.10. DEATH_like. 1 hit. |
| PANTHER | PTHR10454. Pept_C14_p45. 1 hit. |
| Pfam | PF00619. CARD. 1 hit. PF00656. Peptidase_C14. 1 hit. [Graphical view] |
| PIRSF | PIRSF038001. Caspase_ICE. 1 hit. |
| PRINTS | PR00376. IL1BCENZYME. |
| SMART | SM00114. CARD. 1 hit. SM00115. CASc. 1 hit. [Graphical view] |
| PROSITE | PS50209. CARD. 1 hit. PS01122. CASPASE_CYS. 1 hit. PS01121. CASPASE_HIS. 1 hit. PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| LinkHub | P49662. |
| NextBio | 3484. |
| SOURCE | Search... |
Entry information
| Entry name | CASP4_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P49662 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 11 Human chromosome 11: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


