P49657 (MNB_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase minibrain
EC=2.7.12.1

Gene names

Name:	mnb
ORF Names:	CG7826

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	908 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Role in the specific control of proper proliferation of optic lobe neuronal progeny. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Nucleus Potential.
Tissue specificity	In ventral nerve cord and supraesophageal ganglion of embryos. Is most prominent in the mushroom body neuropil and the outer proliferation center of the optic lobes in third instar larvae. Ref.1
Developmental stage	Isoform A and isoform C are present mainly in embryos and pupae. By contrast, isoform D appears to be expressed most markedly in third instar larvae and pupae. Ref.1
Disruption phenotype	Flies exhibit a specific and marked size reduction of the optic lobes and central brain hemispheres but no major alteration in neuronal architecture can be found. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence CAA50065.1 differs from that shown. Reason: Frameshift at position 30. The sequence CAA50068.1 differs from that shown. Reason: Frameshift at position 30. The sequence CAA50069.1 differs from that shown. Reason: Frameshift at position 30.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW circadian rhythm Inferred from genetic interaction. Source: FlyBase nervous system development Inferred from mutant phenotype Ref.1. Source: FlyBase olfactory learning Inferred from mutant phenotype. Source: FlyBase visual behavior Inferred from mutant phenotype Ref.1. Source: FlyBase
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine/tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P49657-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform C (identifier: P49657-3)

The sequence of this isoform differs from the canonical sequence as follows:
     601-607: GLLMHSV → VRRIVRI
     608-908: Missing.

Isoform D (identifier: P49657-2)

The sequence of this isoform differs from the canonical sequence as follows:
     601-604: GLLM → DDRR
     605-908: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 908	908	Serine/threonine-protein kinase minibrain		PRO_0000086340
Regions
	Domain	164 – 484	321	Protein kinase
	Nucleotide binding	170 – 178	9	ATP By similarity
	Motif	121 – 139	19	Bipartite nuclear localization signal
	Compositional bias	496 – 565	70	Gly-rich
	Compositional bias	655 – 691	37	Asn-rich
	Compositional bias	747 – 757	11	Ala-rich
	Compositional bias	832 – 893	62	Ser-rich
Sites
	Active site	292	1	Proton acceptor By similarity
	Binding site	193	1	ATP By similarity
Natural variations
	Alternative sequence	601 – 607	7	GLLMHSV → VRRIVRI in isoform C.		VSP_004915
	Alternative sequence	601 – 604	4	GLLM → DDRR in isoform D.		VSP_004913
	Alternative sequence	605 – 908	304	Missing in isoform D.		VSP_004914
	Alternative sequence	608 – 908	301	Missing in isoform C.		VSP_004916
	Natural variant	191	1	A → T in mnb1; reduced brain volume.
Experimental info
	Sequence conflict	45 – 46	2	HA → S in CAA50065. Ref.1
	Sequence conflict	45 – 46	2	HA → S in CAA50068. Ref.1
	Sequence conflict	45 – 46	2	HA → S in CAA50069. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified August 16, 2005. Version 2. Checksum: FFE6841F42F0BAD8	FASTA	908	95,903
	10 20 30 40 50 60 MYRLEDTNSG GVMDKNKQKL SAYGSSGGSV DAAQGSGSGG GRQRHAPLYG RFVDAEDLPA 70 80 90 100 110 120 THRDVMHHHS SPSSSSEVRA MQARIPNHFR EPASGPLRKL SVDLIKTYKH INEVYYAKKK 130 140 150 160 170 180 RRAQQTQGDD DSSNKKERKL YNDGYDDDNH DYIIKNGEKF LDRYEIDSLI GKGSFGQVVK 190 200 210 220 230 240 AYDHEEQCHV AIKIIKNKKP FLNQAQIEVK LLEMMNRADA ENKYYIVKLK RHFMWRNHLC 250 260 270 280 290 300 LVFELLSYNL YDLLRNTNFR GVSLNLTRKF AQQLCTALLF LSTPELNIIH CDLKPENILL 310 320 330 340 350 360 CNPKRSAIKI VDFGSSCQLG QRIYHYIQSR FYRSPEVLLG IQYDLAIDMW SLGCILVEMH 370 380 390 400 410 420 TGEPLFSGCN EVDQMNKIVE VLGMPPKYLL DQAHKTRKFF DKIVADGSYV LKKNQNGRKY 430 440 450 460 470 480 KPPGSRKLHD ILGVETGGPG GRRLDEPGHS VSDYLKFKDL ILRMLDFDPK TRVTPYYALQ 490 500 510 520 530 540 HNFFKRTADE ATNTSGAGAT ANAGAGGSGS SGAGGSSGGG VGGGLGASNS SSGAVSSSSA 550 560 570 580 590 600 AAPTAATAAA TAAGSSGSGS SVGGGSSAAQ QQQAMPLPLP LPLPLPPLAG PGGASDGQCH 610 620 630 640 650 660 GLLMHSVAAN AMNNFSALSL QSNAHPPPSL ANSHHSTNSL GSLNHISPGS TGCHNNNSNS 670 680 690 700 710 720 SNNNTRHSRL YGSNMVNMVG HHNSGSSNNH NSISYPHAME CDPPQMPPPP PNGHGRMRVP 730 740 750 760 770 780 AIMQLQPNSY APNSVPYYGN MSSSSVAAAA AAAAAAASHL MMTDSSVISA SAAGGGQGGG 790 800 810 820 830 840 NPGQNPVTPS AAAFLFPSQP AGTLYGTALG SLSDLPLPMP LPMSVPLQLP PSSSSSVSSG 850 860 870 880 890 900 SASVGSGGVG VGVVGQRRHI TGPAAQVGIS QSVGSGSSGS ATGASSSDAS SSSPMVGVCV QQNPVVIH
	Isoform C [UniParc]. Checksum: 875512FA576180F3	FASTA	607	66,274
	10 20 30 40 50 60 MYRLEDTNSG GVMDKNKQKL SAYGSSGGSV DAAQGSGSGG GRQRHAPLYG RFVDAEDLPA 70 80 90 100 110 120 THRDVMHHHS SPSSSSEVRA MQARIPNHFR EPASGPLRKL SVDLIKTYKH INEVYYAKKK 130 140 150 160 170 180 RRAQQTQGDD DSSNKKERKL YNDGYDDDNH DYIIKNGEKF LDRYEIDSLI GKGSFGQVVK 190 200 210 220 230 240 AYDHEEQCHV AIKIIKNKKP FLNQAQIEVK LLEMMNRADA ENKYYIVKLK RHFMWRNHLC 250 260 270 280 290 300 LVFELLSYNL YDLLRNTNFR GVSLNLTRKF AQQLCTALLF LSTPELNIIH CDLKPENILL 310 320 330 340 350 360 CNPKRSAIKI VDFGSSCQLG QRIYHYIQSR FYRSPEVLLG IQYDLAIDMW SLGCILVEMH 370 380 390 400 410 420 TGEPLFSGCN EVDQMNKIVE VLGMPPKYLL DQAHKTRKFF DKIVADGSYV LKKNQNGRKY 430 440 450 460 470 480 KPPGSRKLHD ILGVETGGPG GRRLDEPGHS VSDYLKFKDL ILRMLDFDPK TRVTPYYALQ 490 500 510 520 530 540 HNFFKRTADE ATNTSGAGAT ANAGAGGSGS SGAGGSSGGG VGGGLGASNS SSGAVSSSSA 550 560 570 580 590 600 AAPTAATAAA TAAGSSGSGS SVGGGSSAAQ QQQAMPLPLP LPLPLPPLAG PGGASDGQCH VRRIVRI
	Isoform D [UniParc]. Checksum: 7E077F3893AFE249	FASTA	604	65,923
	10 20 30 40 50 60 MYRLEDTNSG GVMDKNKQKL SAYGSSGGSV DAAQGSGSGG GRQRHAPLYG RFVDAEDLPA 70 80 90 100 110 120 THRDVMHHHS SPSSSSEVRA MQARIPNHFR EPASGPLRKL SVDLIKTYKH INEVYYAKKK 130 140 150 160 170 180 RRAQQTQGDD DSSNKKERKL YNDGYDDDNH DYIIKNGEKF LDRYEIDSLI GKGSFGQVVK 190 200 210 220 230 240 AYDHEEQCHV AIKIIKNKKP FLNQAQIEVK LLEMMNRADA ENKYYIVKLK RHFMWRNHLC 250 260 270 280 290 300 LVFELLSYNL YDLLRNTNFR GVSLNLTRKF AQQLCTALLF LSTPELNIIH CDLKPENILL 310 320 330 340 350 360 CNPKRSAIKI VDFGSSCQLG QRIYHYIQSR FYRSPEVLLG IQYDLAIDMW SLGCILVEMH 370 380 390 400 410 420 TGEPLFSGCN EVDQMNKIVE VLGMPPKYLL DQAHKTRKFF DKIVADGSYV LKKNQNGRKY 430 440 450 460 470 480 KPPGSRKLHD ILGVETGGPG GRRLDEPGHS VSDYLKFKDL ILRMLDFDPK TRVTPYYALQ 490 500 510 520 530 540 HNFFKRTADE ATNTSGAGAT ANAGAGGSGS SGAGGSSGGG VGGGLGASNS SSGAVSSSSA 550 560 570 580 590 600 AAPTAATAAA TAAGSSGSGS SVGGGSSAAQ QQQAMPLPLP LPLPLPPLAG PGGASDGQCH DDRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Minibrain: a new protein kinase family involved in postembryonic neurogenesis in Drosophila." Tejedor F., Zhu X.R., Kaltenbach E., Ackermann A., Baumann A., Canal I., Heisenberg M., Fischbach K.F., Pongs O. Neuron 14:287-301(1995) [PubMed: 7857639] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; C AND D), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE. Strain: Berlin.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 21-908 (ISOFORM A). Strain: Berkeley. Tissue: Larva and Pupae.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X70794 mRNA. Translation: CAA50065.1. Frameshift. X70798 mRNA. Translation: CAA50068.1. Frameshift. X70799 mRNA. Translation: CAA50069.1. Frameshift. AE014298 Genomic DNA. Translation: AAF48777.3. AE014298 Genomic DNA. Translation: AAN09442.1. AE014298 Genomic DNA. Translation: AAX52504.1. BT015255 mRNA. Translation: AAT94484.1. Sequence problems.
RefSeq	NP_001014750.1. NM_001014750.1. NP_728104.1. NM_167581.2. NP_728106.1. NM_170658.2.
UniGene	Dm.3606.
3D structure databases
ProteinModelPortal	P49657.
SMR	P49657. Positions 153-485.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49657. 49 interactions.
MINT	MINT-746543.
STRING	P49657.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0300508; FBpp0289735; FBgn0259168.
GeneID	32771.
KEGG	dme:Dmel_CG42273.
NMPDR	fig|7227.3.peg.18397.
UCSC	CG42273-RB. d. melanogaster.
Organism-specific databases
CTD	32771.
FlyBase	FBgn0259168. mnb.
Phylogenomic databases
eggNOG	inNOG06585.
GeneTree	EMGT00050000001142.
InParanoid	P49657.
OMA	LANSHHS.
OrthoDB	EOG4FBG85.
PhylomeDB	P49657.
Enzyme and pathway databases
BRENDA	2.7.12.1. 1994.
Gene expression databases
ArrayExpress	P49657.
Bgee	P49657.
GermOnline	CG7826. Drosophila melanogaster.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	780288.

Entry information

Entry name

MNB_DROME

Accession

Primary (citable) accession number: P49657
Secondary accession number(s): Q59E39 , Q6AWJ3, Q9I7R8, Q9VX07

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	August 16, 2005
Last modified:	January 25, 2012
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project